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The map label for this gene is ramA [H]

Identifier: 119386511

GI number: 119386511

Start: 941360

End: 942220

Strand: Direct

Name: ramA [H]

Synonym: Pden_3804

Alternate gene names: 119386511

Gene position: 941360-942220 (Clockwise)

Preceding gene: 119386510

Following gene: 119386512

Centisome position: 54.41

GC content: 68.29

Gene sequence:

>861_bases
ATGATCGCCGCGAGCCTGGTCCAGATGGACGTGGCGGCGCTGGACCCCGCCCGGAACCTGCGCCGCATCCATGACCATAT
CGCCGCCGAGGCGGGACGCGGCGCGCAGTTGATCCTGTTTCCCGAACTCGCCAATACCGGCTATGTCGAGCCGCTGGTCC
CCGGCGGGGCGATGACCCTGCCGCCGGCGGATTATGCCGGCGCGCTGCATCGCGCCTGCGCCGCGCCGGACGGGCCCGAG
ATCGCCTCGCTGGCCGAGGCGGCGCAGCGGCACGGCACCCATGTCGTCATCGGCATGGGCCTGCGCGATCCGCTGCGCGA
GGGCGTGATGCGCAACTGCTCGCTGCTGATCGGTCCGCAGGGGGTTCTGGGCTGCTATGCCAAGATCCACCAATGGCAGA
ACGAAAAGCTGTATTTCACCCGCGGCGACCGGATCGATAGCTTTGCCTTCGGCGAGACGCGGCTGGGGATGCAGATCTGC
TACGATATCCGCTTTCCCGAGATCACCCGCATCATGGCCATGCAGGGCGCGGGGATCGTGACCTCGGTCTGGGCCTCGTT
CGGCGCCGAGGATGCGCCGGTCCCCGACGAGGCGCTGTTCCTGCACCGCGCCTATACGCGCGCGACCGAGAACGGCGTAT
TCTTCCTTAGCTGCAACCGCAGCGGCAGCCATGGCGGGCAGCGCTTTTTCGGGCGCTCCTGCGCGCTGGCGCCCGACGGC
GCCGTGCTGGGTGCGCTGGACCATGATGGCGAGGACGTGCTGCGGGTCGAGATCGACCTGGCGCAGATCGCCCGCTATCG
CGGCTTCACCGGCATCTGGGCCGATCGCGCGCCCGAGATCTATGCGAAATACTTTCCCTGA

Upstream 100 bases:

>100_bases
TGCTGGCCCATCCGCGCCACGACTATACCCGCGCGCTGATTGCCGCCGCACCCGGCCGCGACTGGGATTTTTCCACGGGC
CGCCGGATCGAGGTGCCGGC

Downstream 100 bases:

>100_bases
CCGGAGCTTTTCATGACTTCTTCCTGCCCGCCGCTTGCGGATGCCGACTGGCCCGCAGAGATCCTCGACCTGCGCCAGGG
TTTTGCCGGGGCGCTGAACG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MIAASLVQMDVAALDPARNLRRIHDHIAAEAGRGAQLILFPELANTGYVEPLVPGGAMTLPPADYAGALHRACAAPDGPE
IASLAEAAQRHGTHVVIGMGLRDPLREGVMRNCSLLIGPQGVLGCYAKIHQWQNEKLYFTRGDRIDSFAFGETRLGMQIC
YDIRFPEITRIMAMQGAGIVTSVWASFGAEDAPVPDEALFLHRAYTRATENGVFFLSCNRSGSHGGQRFFGRSCALAPDG
AVLGALDHDGEDVLRVEIDLAQIARYRGFTGIWADRAPEIYAKYFP

Sequences:

>Translated_286_residues
MIAASLVQMDVAALDPARNLRRIHDHIAAEAGRGAQLILFPELANTGYVEPLVPGGAMTLPPADYAGALHRACAAPDGPE
IASLAEAAQRHGTHVVIGMGLRDPLREGVMRNCSLLIGPQGVLGCYAKIHQWQNEKLYFTRGDRIDSFAFGETRLGMQIC
YDIRFPEITRIMAMQGAGIVTSVWASFGAEDAPVPDEALFLHRAYTRATENGVFFLSCNRSGSHGGQRFFGRSCALAPDG
AVLGALDHDGEDVLRVEIDLAQIARYRGFTGIWADRAPEIYAKYFP
>Mature_286_residues
MIAASLVQMDVAALDPARNLRRIHDHIAAEAGRGAQLILFPELANTGYVEPLVPGGAMTLPPADYAGALHRACAAPDGPE
IASLAEAAQRHGTHVVIGMGLRDPLREGVMRNCSLLIGPQGVLGCYAKIHQWQNEKLYFTRGDRIDSFAFGETRLGMQIC
YDIRFPEITRIMAMQGAGIVTSVWASFGAEDAPVPDEALFLHRAYTRATENGVFFLSCNRSGSHGGQRFFGRSCALAPDG
AVLGALDHDGEDVLRVEIDLAQIARYRGFTGIWADRAPEIYAKYFP

Specific function: Hydrolyzes (R)-piperazine-2-carboxamide and (R)- piperazine-2-tert-butylcarboxamide with strict R- stereoselectivity. Also active towards beta-alaninamide, piperidine-3-carboxmide, D-glutaminamide and slightly active towards L-glutaminamide and piperidine

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1786412, Length=277, Percent_Identity=22.0216606498195, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.100 [H]

Molecular weight: Translated: 31041; Mature: 31041

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAASLVQMDVAALDPARNLRRIHDHIAAEAGRGAQLILFPELANTGYVEPLVPGGAMTL
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEC
PPADYAGALHRACAAPDGPEIASLAEAAQRHGTHVVIGMGLRDPLREGVMRNCSLLIGPQ
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCEEEECCC
GVLGCYAKIHQWQNEKLYFTRGDRIDSFAFGETRLGMQICYDIRFPEITRIMAMQGAGIV
HHHHHHHHHHHCCCCEEEEEECCCCCCEECCCHHCCEEEEEECCCCHHHHHHHHCCCCHH
TSVWASFGAEDAPVPDEALFLHRAYTRATENGVFFLSCNRSGSHGGQRFFGRSCALAPDG
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHCCCCCCCCCC
AVLGALDHDGEDVLRVEIDLAQIARYRGFTGIWADRAPEIYAKYFP
CEEEECCCCCCCEEEEEECHHHHHHHCCCCCCCCCCCCCHHHCCCC
>Mature Secondary Structure
MIAASLVQMDVAALDPARNLRRIHDHIAAEAGRGAQLILFPELANTGYVEPLVPGGAMTL
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEC
PPADYAGALHRACAAPDGPEIASLAEAAQRHGTHVVIGMGLRDPLREGVMRNCSLLIGPQ
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCEEEECCC
GVLGCYAKIHQWQNEKLYFTRGDRIDSFAFGETRLGMQICYDIRFPEITRIMAMQGAGIV
HHHHHHHHHHHCCCCEEEEEECCCCCCEECCCHHCCEEEEEECCCCHHHHHHHHCCCCHH
TSVWASFGAEDAPVPDEALFLHRAYTRATENGVFFLSCNRSGSHGGQRFFGRSCALAPDG
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHCCCCCCCCCC
AVLGALDHDGEDVLRVEIDLAQIARYRGFTGIWADRAPEIYAKYFP
CEEEECCCCCCCEEEEEECHHHHHHHCCCCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA