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The map label for this gene is alkB [H]

Identifier: 119386500

GI number: 119386500

Start: 927552

End: 928208

Strand: Direct

Name: alkB [H]

Synonym: Pden_3793

Alternate gene names: 119386500

Gene position: 927552-928208 (Clockwise)

Preceding gene: 119386498

Following gene: 119386503

Centisome position: 53.61

GC content: 70.62

Gene sequence:

>657_bases
ATGACAATTCCGTGGGAGAACGGCATGGAGCCAGACCTGTTCGGAACCCGTCGCGACGAGCCCATCGGCCCGGGCGCCAT
GATCCTGCGCGGCCTTGCCTCGGCCCCCGAGATCCTGGCCGAGGTCGAGCGCATCGCCGCCCTTTCGCCCTTCCGCCACA
TGCTGACCCCGGGCGGGCGCCGGATCGGTGTCGAGATGACGAATTGCGGTGCGCTCGGCTGGGTCAGCGACCGGCGCGGC
TATCGCTATGAGCCCTGCGATCCGCTGACCGGGCGACCCTGGCCGGCGATGCCCGCACGCTTCCTGCAACTGGCGGATGA
GGCGGCCGCGCGGGCGGGGTTCCCCGGCTTCCGGCCCGATGCCTGCCTGGTCAACCGCTATGTGCCGGGGGTCAAGATGG
GATTGCACCAGGATCGCGACGAGGCGGGATTCGATGCGCCCATCGTCTCGGTCTCGCTGGGCTTGCCGGCGACGTTCCAG
TTCGGCGGTCCCGAGCGCAGCGATCCGGTGGCGAAGCATGTGCTGGACCATGGCGATGTCGTGGTCTGGGGCGGGCCGGC
GCGGCTGGCCTGGCACGGCATCCTGACGCTGAGACGGGCCGAGCATCCGCTGACCGGCGCCGCGCGCATCAACCTGACCT
TTCGCCGCGCCGGCTAG

Upstream 100 bases:

>100_bases
CGCAAGGGCGATTGCGGCAAGGGGGCGGAGCATGGCAAGAGTCCTTTGCTGTGGCCAGACCTGAAAGCCATTGCCGGGCG
CGCGGGTCAAGATCCGCGCC

Downstream 100 bases:

>100_bases
GCCGTCGCAGGCTTTTTCCGTTTTTCAAATACCCCGGGGGAGCGCGGCGTCCGCCCCATCAAGGGGCGGGCGCCGCGCGG
GGGCGGAGCCCCTTTACTTC

Product: 2OG-Fe(II) oxygenase

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTIPWENGMEPDLFGTRRDEPIGPGAMILRGLASAPEILAEVERIAALSPFRHMLTPGGRRIGVEMTNCGALGWVSDRRG
YRYEPCDPLTGRPWPAMPARFLQLADEAAARAGFPGFRPDACLVNRYVPGVKMGLHQDRDEAGFDAPIVSVSLGLPATFQ
FGGPERSDPVAKHVLDHGDVVVWGGPARLAWHGILTLRRAEHPLTGAARINLTFRRAG

Sequences:

>Translated_218_residues
MTIPWENGMEPDLFGTRRDEPIGPGAMILRGLASAPEILAEVERIAALSPFRHMLTPGGRRIGVEMTNCGALGWVSDRRG
YRYEPCDPLTGRPWPAMPARFLQLADEAAARAGFPGFRPDACLVNRYVPGVKMGLHQDRDEAGFDAPIVSVSLGLPATFQ
FGGPERSDPVAKHVLDHGDVVVWGGPARLAWHGILTLRRAEHPLTGAARINLTFRRAG
>Mature_217_residues
TIPWENGMEPDLFGTRRDEPIGPGAMILRGLASAPEILAEVERIAALSPFRHMLTPGGRRIGVEMTNCGALGWVSDRRGY
RYEPCDPLTGRPWPAMPARFLQLADEAAARAGFPGFRPDACLVNRYVPGVKMGLHQDRDEAGFDAPIVSVSLGLPATFQF
GGPERSDPVAKHVLDHGDVVVWGGPARLAWHGILTLRRAEHPLTGAARINLTFRRAG

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Escherichia coli, GI1788541, Length=211, Percent_Identity=55.9241706161137, Blast_Score=243, Evalue=5e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23737; Mature: 23605

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPWENGMEPDLFGTRRDEPIGPGAMILRGLASAPEILAEVERIAALSPFRHMLTPGGR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
RIGVEMTNCGALGWVSDRRGYRYEPCDPLTGRPWPAMPARFLQLADEAAARAGFPGFRPD
EEEEEEECCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
ACLVNRYVPGVKMGLHQDRDEAGFDAPIVSVSLGLPATFQFGGPERSDPVAKHVLDHGDV
HHHHHHCCCCCHHCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHCCCCE
VVWGGPARLAWHGILTLRRAEHPLTGAARINLTFRRAG
EEECCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure 
TIPWENGMEPDLFGTRRDEPIGPGAMILRGLASAPEILAEVERIAALSPFRHMLTPGGR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
RIGVEMTNCGALGWVSDRRGYRYEPCDPLTGRPWPAMPARFLQLADEAAARAGFPGFRPD
EEEEEEECCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
ACLVNRYVPGVKMGLHQDRDEAGFDAPIVSVSLGLPATFQFGGPERSDPVAKHVLDHGDV
HHHHHHCCCCCHHCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCHHHHHHHCCCCE
VVWGGPARLAWHGILTLRRAEHPLTGAARINLTFRRAG
EEECCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]