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The map label for this gene is paaF [C]

Identifier: 119386350

GI number: 119386350

Start: 792801

End: 793583

Strand: Direct

Name: paaF [C]

Synonym: Pden_3642

Alternate gene names: 119386350

Gene position: 792801-793583 (Clockwise)

Preceding gene: 119386349

Following gene: 119386351

Centisome position: 45.82

GC content: 71.65

Gene sequence:

>783_bases
ATGGAAACGATCCGCATCGACACCGACGCCCGCGGCGTGGCGCATCTGTGGCTCGCGCGCCCCGAGAAGCACAATGCGCT
TTCGGCGCAGATGATCCGCGAATTGACCCGGGCGGCGCAGGCGCTCGGGGCCGATCCGGCGGTGCGCGTCGTTGTGCTGG
CGGCCGAGGGGCGCAGTTTCTGCGCCGGCGGCGATCTGGGCTGGATGCAGGAGCAGATGCGCGCCGATGCCGAAACCCGC
CGCCAGGGCGCGCGCGAGCTTGCCATGATGCTGAACGCGCTCAACACCCTGCCCAAGCCGTTGATCGGCCGGGTGCAGGG
CAATGCCTTCGGCGGCGGGATCGGCATGATCTCGGTCTGCGACGTGGCCGTGGCGGTGCCCGAGGCGCGCTTCGGCCTGA
CCGAGACGCGGCTGGGCCTCATCCCCGCCACCATCGGCCCCTATGTCCTGGCCCGCATGGGCGAGGACAAGGCGCGGCGC
GTCTTCATGTCCGCGCGGCTGTTCGGCGCCGAGGAGGCGTTGCGGCTGAACCTGCTGGCAAGCCTTGCCGATGCCGAGAC
GCTGGACGCCGCCGTCGAGGCCGAGGTCGTGCCCTATCTCTCCTGCGCCCCGGGCGCGGTGGCGGCCGCCAAGCGCCTGG
CCCGCCGCCTCGGCTCCCCCATCGGCGAGGCCGAAATCGAGGCCAGCATCCAGGCCCTCGTCGAAGTCTGGGAGGGCGAC
GAGGCCCCCGAGGGCATCGCCGCCTTCTTCGACAAGCGCAAACCGCGCTGGCATGTCGGCTGA

Upstream 100 bases:

>100_bases
CCGCGCACGGCCACGACACCGGGCTGGACATGGCGGTGATCGAACAGGCCGCCGCCATGGCCCGCACCATGAGGGCCCGC
ACCATGAGGGGAGGAGCATG

Downstream 100 bases:

>100_bases
GGCGCCGACCTTCGTCTTCTTCGTTCTGGAAATACTCATCGGCAGGCGTCCCGCAGCGCCCAGGTTGCGGATTTGGTCCC
CATATCGACGTCGCGCCCGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
METIRIDTDARGVAHLWLARPEKHNALSAQMIRELTRAAQALGADPAVRVVVLAAEGRSFCAGGDLGWMQEQMRADAETR
RQGARELAMMLNALNTLPKPLIGRVQGNAFGGGIGMISVCDVAVAVPEARFGLTETRLGLIPATIGPYVLARMGEDKARR
VFMSARLFGAEEALRLNLLASLADAETLDAAVEAEVVPYLSCAPGAVAAAKRLARRLGSPIGEAEIEASIQALVEVWEGD
EAPEGIAAFFDKRKPRWHVG

Sequences:

>Translated_260_residues
METIRIDTDARGVAHLWLARPEKHNALSAQMIRELTRAAQALGADPAVRVVVLAAEGRSFCAGGDLGWMQEQMRADAETR
RQGARELAMMLNALNTLPKPLIGRVQGNAFGGGIGMISVCDVAVAVPEARFGLTETRLGLIPATIGPYVLARMGEDKARR
VFMSARLFGAEEALRLNLLASLADAETLDAAVEAEVVPYLSCAPGAVAAAKRLARRLGSPIGEAEIEASIQALVEVWEGD
EAPEGIAAFFDKRKPRWHVG
>Mature_260_residues
METIRIDTDARGVAHLWLARPEKHNALSAQMIRELTRAAQALGADPAVRVVVLAAEGRSFCAGGDLGWMQEQMRADAETR
RQGARELAMMLNALNTLPKPLIGRVQGNAFGGGIGMISVCDVAVAVPEARFGLTETRLGLIPATIGPYVLARMGEDKARR
VFMSARLFGAEEALRLNLLASLADAETLDAAVEAEVVPYLSCAPGAVAAAKRLARRLGSPIGEAEIEASIQALVEVWEGD
EAPEGIAAFFDKRKPRWHVG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=27.0588235294118, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI4502327, Length=270, Percent_Identity=30, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI31542718, Length=254, Percent_Identity=27.9527559055118, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI37594471, Length=193, Percent_Identity=27.4611398963731, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI37594469, Length=195, Percent_Identity=27.1794871794872, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI70995211, Length=228, Percent_Identity=28.0701754385965, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI45643119, Length=255, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260275230, Length=255, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260274832, Length=255, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221136756, Length=247, Percent_Identity=24.6963562753036, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI221136753, Length=247, Percent_Identity=24.6963562753036, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI221307494, Length=247, Percent_Identity=24.6963562753036, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI68989263, Length=170, Percent_Identity=30.5882352941176, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI25453481, Length=237, Percent_Identity=23.2067510548523, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI51538573, Length=237, Percent_Identity=23.2067510548523, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI116235487, Length=248, Percent_Identity=22.5806451612903, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757966, Length=230, Percent_Identity=23.9130434782609, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI51538581, Length=230, Percent_Identity=23.9130434782609, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757968, Length=237, Percent_Identity=23.2067510548523, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI49355787, Length=237, Percent_Identity=23.2067510548523, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=250, Percent_Identity=32, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=26.8939393939394, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI221142681, Length=252, Percent_Identity=32.1428571428571, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI87082183, Length=241, Percent_Identity=29.045643153527, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790281, Length=188, Percent_Identity=28.7234042553192, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=28.3524904214559, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=186, Percent_Identity=33.8709677419355, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=219, Percent_Identity=30.1369863013699, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=261, Percent_Identity=29.1187739463602, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=204, Percent_Identity=28.921568627451, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=250, Percent_Identity=25.6, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=251, Percent_Identity=27.4900398406374, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=197, Percent_Identity=28.4263959390863, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=239, Percent_Identity=25.9414225941423, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=187, Percent_Identity=28.8770053475936, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=187, Percent_Identity=28.8770053475936, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI25141363, Length=224, Percent_Identity=26.3392857142857, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=170, Percent_Identity=27.6470588235294, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24653139, Length=265, Percent_Identity=32.8301886792453, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=26.6932270916335, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI20129971, Length=187, Percent_Identity=29.9465240641711, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653477, Length=187, Percent_Identity=29.9465240641711, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24654903, Length=199, Percent_Identity=26.6331658291457, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI19920382, Length=200, Percent_Identity=28, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27820; Mature: 27820

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIRIDTDARGVAHLWLARPEKHNALSAQMIRELTRAAQALGADPAVRVVVLAAEGRSF
CCEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCE
CAGGDLGWMQEQMRADAETRRQGARELAMMLNALNTLPKPLIGRVQGNAFGGGIGMISVC
ECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHH
DVAVAVPEARFGLTETRLGLIPATIGPYVLARMGEDKARRVFMSARLFGAEEALRLNLLA
HHHHHCCHHHCCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHH
SLADAETLDAAVEAEVVPYLSCAPGAVAAAKRLARRLGSPIGEAEIEASIQALVEVWEGD
HHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
EAPEGIAAFFDKRKPRWHVG
CCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
METIRIDTDARGVAHLWLARPEKHNALSAQMIRELTRAAQALGADPAVRVVVLAAEGRSF
CCEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCE
CAGGDLGWMQEQMRADAETRRQGARELAMMLNALNTLPKPLIGRVQGNAFGGGIGMISVC
ECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHH
DVAVAVPEARFGLTETRLGLIPATIGPYVLARMGEDKARRVFMSARLFGAEEALRLNLLA
HHHHHCCHHHCCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHH
SLADAETLDAAVEAEVVPYLSCAPGAVAAAKRLARRLGSPIGEAEIEASIQALVEVWEGD
HHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
EAPEGIAAFFDKRKPRWHVG
CCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA