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The map label for this gene is pcaB [H]

Identifier: 119386207

GI number: 119386207

Start: 641701

End: 643002

Strand: Direct

Name: pcaB [H]

Synonym: Pden_3496

Alternate gene names: 119386207

Gene position: 641701-643002 (Clockwise)

Preceding gene: 119386206

Following gene: 119386208

Centisome position: 37.09

GC content: 74.5

Gene sequence:

>1302_bases
ATGGCGGTGGGCACCTTGTTCGGACAGCTTTACGGCGACGACGAGATGGCAGCGATCCTGTCGGATCGCTCGGCCATCCT
GGCCATGCTGCGGGTCGAGGTCGCATTGGCCCGCGCGCAGGCCGATCTGGGGGCGATCCCGGCGGCGGCGGCCGAGGTGA
TCGCCAAGGCGGCGGAGGCACTGGACCCCGACCCGGCGGAACTGGCCGCGGCGGTGGCCCGGGCCGGCATTACCGCGCAG
CCGGTCATCGCGGCGCTGAAATCCGCGGCGGGCGAGGCCGGGGCATTCGCCCATTACGGCGCCACCTCGCAGGACATCGT
GGACACGGCGCTGGCGCTGCAACTGGTGGCGGCGCTGGACCTGTTGGGCGGGCGGCTGGACCGACTGACGGCGCTGCTGG
TGCAGAAGGCCGCCGATCATGCCGACCTGCCGATCCCGGCGCGCACCCGCCACCAGATCGCCGCACCGACCACGCTGGGC
GCCAAGATCGCGGTCTGGCACGGGATGGCGGCGCGCAACCGGCAACGGCTGGACGAGCTGCGGCCGCGGCTGCTGCTGGT
CTCGCTGCACGGTGCGGCGGGGACCGAGGCGGCGCTGGCCGGCCACGGCCCGGCGCTGCGCGCCTCGATGGCGGCGGCGC
TGGGGCTGGGGGTGCCGGCGGGGCCCTGGCACGCGGCCCGCGACGGCATCGCGGAACTGGCGGGATGGCTGTCGCTGGTC
ACGGGCGCGCTTGGCAAGATGGGCCTTGACCTGGTGCAGCTTGGCCAAAGCGAAATCGCCGAGGTTGCGGCCGGGGCAGG
GGGCGGCTCGTCCACCATGCCGCAAAAGCAGAACCCCGTTGCGGCCGAGGCGCTGGCGACGCTGGCGCGGCTGAATGCCG
GCGATCTGGCGGGGATGCATCAGGCGCTGGTCCATGCGCAGGAACGCGACGGCATCGCGCTGGGGCTGGAATGGAACCTG
CTGCCGGGAATGCTGGAACGCTCGGCCGCGGCGCTGCGGATCGCTTGCGATCTGGCCGGAACGCTGGTTCCCCGGCCCGA
CCGTATCGCCGCCACATTCGCCGCCGACCGCGGACGGATGGCGGCCGAGGCCGCAGGCTTTCTGCTGGCCCAGACCCTGC
CCCGCGCCGAGGCGCTGCGGATCGTGGCGCAGGCGCTGGCGCAGGTCGCCGGGGGCGAGGCGCCCACGCTGGCCGATGCG
CTGGCGCGGCTTGCGCCCGGCCGGGACTGGGCCGAGGCGCTGCGGCCCGAAAACCTGACCGGCGCCGCGCCAGACCAGGC
GCGCGCCGCACGACCGATCTGA

Upstream 100 bases:

>100_bases
CCGCGCTGGTCGGCCGTGCGGTCGAACTGTGCGAGAAATACGAACGCCCCGTCGCGACATGGCGGCAGGCGCGGCAGATC
CTTGGGCTGCGGATGGTCTG

Downstream 100 bases:

>100_bases
ACAACGCAACGGGAGGAAACCATGAAAACCCTGATCCTGTCGGCCATCGCCTCGCTTTCGCTGGGCGGCGCGGCCTTTGC
CGCCGATGTGACGCTGCGCG

Product: fumarate lyase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MAVGTLFGQLYGDDEMAAILSDRSAILAMLRVEVALARAQADLGAIPAAAAEVIAKAAEALDPDPAELAAAVARAGITAQ
PVIAALKSAAGEAGAFAHYGATSQDIVDTALALQLVAALDLLGGRLDRLTALLVQKAADHADLPIPARTRHQIAAPTTLG
AKIAVWHGMAARNRQRLDELRPRLLLVSLHGAAGTEAALAGHGPALRASMAAALGLGVPAGPWHAARDGIAELAGWLSLV
TGALGKMGLDLVQLGQSEIAEVAAGAGGGSSTMPQKQNPVAAEALATLARLNAGDLAGMHQALVHAQERDGIALGLEWNL
LPGMLERSAAALRIACDLAGTLVPRPDRIAATFAADRGRMAAEAAGFLLAQTLPRAEALRIVAQALAQVAGGEAPTLADA
LARLAPGRDWAEALRPENLTGAAPDQARAARPI

Sequences:

>Translated_433_residues
MAVGTLFGQLYGDDEMAAILSDRSAILAMLRVEVALARAQADLGAIPAAAAEVIAKAAEALDPDPAELAAAVARAGITAQ
PVIAALKSAAGEAGAFAHYGATSQDIVDTALALQLVAALDLLGGRLDRLTALLVQKAADHADLPIPARTRHQIAAPTTLG
AKIAVWHGMAARNRQRLDELRPRLLLVSLHGAAGTEAALAGHGPALRASMAAALGLGVPAGPWHAARDGIAELAGWLSLV
TGALGKMGLDLVQLGQSEIAEVAAGAGGGSSTMPQKQNPVAAEALATLARLNAGDLAGMHQALVHAQERDGIALGLEWNL
LPGMLERSAAALRIACDLAGTLVPRPDRIAATFAADRGRMAAEAAGFLLAQTLPRAEALRIVAQALAQVAGGEAPTLADA
LARLAPGRDWAEALRPENLTGAAPDQARAARPI
>Mature_432_residues
AVGTLFGQLYGDDEMAAILSDRSAILAMLRVEVALARAQADLGAIPAAAAEVIAKAAEALDPDPAELAAAVARAGITAQP
VIAALKSAAGEAGAFAHYGATSQDIVDTALALQLVAALDLLGGRLDRLTALLVQKAADHADLPIPARTRHQIAAPTTLGA
KIAVWHGMAARNRQRLDELRPRLLLVSLHGAAGTEAALAGHGPALRASMAAALGLGVPAGPWHAARDGIAELAGWLSLVT
GALGKMGLDLVQLGQSEIAEVAAGAGGGSSTMPQKQNPVAAEALATLARLNAGDLAGMHQALVHAQERDGIALGLEWNLL
PGMLERSAAALRIACDLAGTLVPRPDRIAATFAADRGRMAAEAAGFLLAQTLPRAEALRIVAQALAQVAGGEAPTLADAL
ARLAPGRDWAEALRPENLTGAAPDQARAARPI

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323391, Length=454, Percent_Identity=26.6519823788546, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI221473854, Length=353, Percent_Identity=25.4957507082153, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI78706858, Length=353, Percent_Identity=25.4957507082153, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24647570, Length=298, Percent_Identity=28.8590604026846, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 43968; Mature: 43837

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVGTLFGQLYGDDEMAAILSDRSAILAMLRVEVALARAQADLGAIPAAAAEVIAKAAEA
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LDPDPAELAAAVARAGITAQPVIAALKSAAGEAGAFAHYGATSQDIVDTALALQLVAALD
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHH
LLGGRLDRLTALLVQKAADHADLPIPARTRHQIAAPTTLGAKIAVWHGMAARNRQRLDEL
HHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHH
RPRLLLVSLHGAAGTEAALAGHGPALRASMAAALGLGVPAGPWHAARDGIAELAGWLSLV
CCEEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
TGALGKMGLDLVQLGQSEIAEVAAGAGGGSSTMPQKQNPVAAEALATLARLNAGDLAGMH
HHHHHHHCHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHH
QALVHAQERDGIALGLEWNLLPGMLERSAAALRIACDLAGTLVPRPDRIAATFAADRGRM
HHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCH
AAEAAGFLLAQTLPRAEALRIVAQALAQVAGGEAPTLADALARLAPGRDWAEALRPENLT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHCCCCCCC
GAAPDQARAARPI
CCCCCHHHCCCCC
>Mature Secondary Structure 
AVGTLFGQLYGDDEMAAILSDRSAILAMLRVEVALARAQADLGAIPAAAAEVIAKAAEA
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LDPDPAELAAAVARAGITAQPVIAALKSAAGEAGAFAHYGATSQDIVDTALALQLVAALD
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHH
LLGGRLDRLTALLVQKAADHADLPIPARTRHQIAAPTTLGAKIAVWHGMAARNRQRLDEL
HHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHH
RPRLLLVSLHGAAGTEAALAGHGPALRASMAAALGLGVPAGPWHAARDGIAELAGWLSLV
CCEEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
TGALGKMGLDLVQLGQSEIAEVAAGAGGGSSTMPQKQNPVAAEALATLARLNAGDLAGMH
HHHHHHHCHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHH
QALVHAQERDGIALGLEWNLLPGMLERSAAALRIACDLAGTLVPRPDRIAATFAADRGRM
HHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCH
AAEAAGFLLAQTLPRAEALRIVAQALAQVAGGEAPTLADALARLAPGRDWAEALRPENLT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHCCCCCCC
GAAPDQARAARPI
CCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8063101 [H]