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The map label for this gene is garL [H]

Identifier: 119386196

GI number: 119386196

Start: 631560

End: 632309

Strand: Direct

Name: garL [H]

Synonym: Pden_3485

Alternate gene names: 119386196

Gene position: 631560-632309 (Clockwise)

Preceding gene: 119386195

Following gene: 119386197

Centisome position: 36.5

GC content: 70.93

Gene sequence:

>750_bases
ATGAGCGGGCTGAAGGCACGGATCAACGCGGGCGAGACCGTCATCGGCACTTTCGTCAAGACGCCCGCATCCCATGTCAT
CGAGATCCTGGGCCTTGCCGGGCTGGATTTCGCGGTGCCCGACCAGGAACATGCGCCGCTGGACCTGGGCGCGATGGACC
AGCTTGCGCTGGCCTCGCGCGCGGCCGGGCTGCCCCTGGTCGCGCGCTGCTGGGGGCAATCGCCGGCGCGGATCTCGCCG
CTGCTGGACCTGGGCTGCGCGGGGGTGATGGTGCCCCATGTCGCGGATCGCGACACGGCGGAAGCCGTCGTGGCCGCGGT
CAAGTTCGGTCGGGGGTCGCGCGGCCTGTCGCCGTCGCCGCGCGCCGGGAATTACGGCACCCTCGCCGCCCCCGCCTATA
CTGCGAAAAGCGACGCCGAAACCGTGGTGATGGTGCAGATCGAGGATAGGGCGGCGCTGGACCGGCTGGACGACATCGCC
AGCGTGCCGGACGTCGATGTGCTGTTCGTCGGTCCCGCCGATCTGTCGCAGTCGCTGGGCTGCGGCTTCCCCTCGGCCGA
GCTTGACGCGGCGATCATTCGCGTGATCGCGGCTGCCCGGCGCGCGGATGTCGCAGCGGGCCTGTTCGTCGGCGACGAAA
GCCAGATCGCCCCCTGGCGCGAACGCGGCGTCAGCGTCTTCGTCTGCGGCTCGGATCAGGGGCTGCTGATGAAGGGCGCG
CGCCGGATCATCGAGGCGGGCCGCGCCTGA

Upstream 100 bases:

>100_bases
TGCGCCAGATGATCGCCATGCTGATCGGGCTGGGCTATTCGGATGCCGATATCCGCAAGATGACCTCGACCATCGGGGCC
GAACTGGTCGGGCTTGCGGC

Downstream 100 bases:

>100_bases
CGCCCGCGCTGACAGGAAAGGATTTTCGCGATGTACAAGGACAAGGATCCCCGTGCGGCCCTGTTCGCCGCGGCCTCGGC
GCCGCCCCGCACCGGCCTGA

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSGLKARINAGETVIGTFVKTPASHVIEILGLAGLDFAVPDQEHAPLDLGAMDQLALASRAAGLPLVARCWGQSPARISP
LLDLGCAGVMVPHVADRDTAEAVVAAVKFGRGSRGLSPSPRAGNYGTLAAPAYTAKSDAETVVMVQIEDRAALDRLDDIA
SVPDVDVLFVGPADLSQSLGCGFPSAELDAAIIRVIAAARRADVAAGLFVGDESQIAPWRERGVSVFVCGSDQGLLMKGA
RRIIEAGRA

Sequences:

>Translated_249_residues
MSGLKARINAGETVIGTFVKTPASHVIEILGLAGLDFAVPDQEHAPLDLGAMDQLALASRAAGLPLVARCWGQSPARISP
LLDLGCAGVMVPHVADRDTAEAVVAAVKFGRGSRGLSPSPRAGNYGTLAAPAYTAKSDAETVVMVQIEDRAALDRLDDIA
SVPDVDVLFVGPADLSQSLGCGFPSAELDAAIIRVIAAARRADVAAGLFVGDESQIAPWRERGVSVFVCGSDQGLLMKGA
RRIIEAGRA
>Mature_248_residues
SGLKARINAGETVIGTFVKTPASHVIEILGLAGLDFAVPDQEHAPLDLGAMDQLALASRAAGLPLVARCWGQSPARISPL
LDLGCAGVMVPHVADRDTAEAVVAAVKFGRGSRGLSPSPRAGNYGTLAAPAYTAKSDAETVVMVQIEDRAALDRLDDIAS
VPDVDVLFVGPADLSQSLGCGFPSAELDAAIIRVIAAARRADVAAGLFVGDESQIAPWRERGVSVFVCGSDQGLLMKGAR
RIIEAGRA

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=245, Percent_Identity=31.0204081632653, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1788578, Length=207, Percent_Identity=32.8502415458937, Blast_Score=97, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 25721; Mature: 25590

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLKARINAGETVIGTFVKTPASHVIEILGLAGLDFAVPDQEHAPLDLGAMDQLALASR
CCCCCCCCCCCCEEEHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AAGLPLVARCWGQSPARISPLLDLGCAGVMVPHVADRDTAEAVVAAVKFGRGSRGLSPSP
HCCCCHHHHHCCCCCCHHCHHHHHCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
RAGNYGTLAAPAYTAKSDAETVVMVQIEDRAALDRLDDIASVPDVDVLFVGPADLSQSLG
CCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHC
CGFPSAELDAAIIRVIAAARRADVAAGLFVGDESQIAPWRERGVSVFVCGSDQGLLMKGA
CCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHCCCEEEEECCCCCHHHHHH
RRIIEAGRA
HHHHHCCCC
>Mature Secondary Structure 
SGLKARINAGETVIGTFVKTPASHVIEILGLAGLDFAVPDQEHAPLDLGAMDQLALASR
CCCCCCCCCCCEEEHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AAGLPLVARCWGQSPARISPLLDLGCAGVMVPHVADRDTAEAVVAAVKFGRGSRGLSPSP
HCCCCHHHHHCCCCCCHHCHHHHHCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
RAGNYGTLAAPAYTAKSDAETVVMVQIEDRAALDRLDDIASVPDVDVLFVGPADLSQSLG
CCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHC
CGFPSAELDAAIIRVIAAARRADVAAGLFVGDESQIAPWRERGVSVFVCGSDQGLLMKGA
CCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHCCCEEEEECCCCCHHHHHH
RRIIEAGRA
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA