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The map label for this gene is hisJ [H]

Identifier: 119356995

GI number: 119356995

Start: 1330034

End: 1330831

Strand: Direct

Name: hisJ [H]

Synonym: Cpha266_1178

Alternate gene names: 119356995

Gene position: 1330034-1330831 (Clockwise)

Preceding gene: 119356990

Following gene: 119356996

Centisome position: 42.44

GC content: 42.11

Gene sequence:

>798_bases
ATGAAAATCATCATTATGAAAATCAATCGCTTAAGCATAGTAATTATGGCGTTTTTTTGCTCCAGCCTCCTCTTGTCAGG
TTGTAGCAAAAGAGAAAAAATCAGTGCGCTTCAGCAATTGAACGGTAAAGAGTTTGCGATCCCTACAGGTACCGTTGCTG
ATCAGCTTGTGCTTTCAAAGCTGCCAAATGCGAAATTCAAGTATTTTAACAGTGTTATGGATGCTGCGCTTGCCGTTAAA
TCAGGAAAGGCAGATGCTGCCGCTTATGATGAGCCTATTCTCAAAAACATTGCTGCTAAAAACAGTGGCCTTGTTGTGCT
TTCAGACATGATTACGGTTGATAACTATGGGTTTGCTGTACGTAAAGATGATACTGAGTTAAAAAAAAACATAGATACTG
TCGTGTTGGACCTTAAGAATAACGGCACTACTGGTCAAATGATGAAGCGCTGGTTCCCGGTATCGGGGAATCCTGCTCCT
ATGCCGGTGATTTCATCAACCGGAAGCAAAGGTATCTTGAGATTGGGCACTGCAAGCGTAACTGAGCCATTTTCTTTTGT
GGATGGTTCGGGTGAAATTGTAGGATATGATATTGAACTTGCCAGATATATTGCCAAAAAACTTGACAAGAAGCTTGTTA
TTGTGAATATGGATTTTGGAGGGATGATTCCTGCCCTGATGTCAAGCAAGGTTGATATGATTGCGGCTTGTATTACCATA
ACAGACGAACGCTCCAGGCAGGTTCTTTTTTCTGAGCCGTATTATATCGGAGGGATTGCTGCGCTTGTAAGGCAGTGA

Upstream 100 bases:

>100_bases
TTCTTACTAATTCCACAGGGAGATTTCCATTCAATACCGTATTATTCACCTTGTTTCTAATTGTTTTTTTCTCGGTTCAT
GAGATTTTTGGCGTAAAAAA

Downstream 100 bases:

>100_bases
CTGGTTTCGATAAGAAAAATTTCTGTACATCAGTAATCTCTTATGAGTTTGGAATGGAACGGTCATAAACAGTGATGACA
TCTTTGCTGCCAGCGGAATA

Product: amino acid ABC transporter periplasmic protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: HBP; p29 [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKIIIMKINRLSIVIMAFFCSSLLLSGCSKREKISALQQLNGKEFAIPTGTVADQLVLSKLPNAKFKYFNSVMDAALAVK
SGKADAAAYDEPILKNIAAKNSGLVVLSDMITVDNYGFAVRKDDTELKKNIDTVVLDLKNNGTTGQMMKRWFPVSGNPAP
MPVISSTGSKGILRLGTASVTEPFSFVDGSGEIVGYDIELARYIAKKLDKKLVIVNMDFGGMIPALMSSKVDMIAACITI
TDERSRQVLFSEPYYIGGIAALVRQ

Sequences:

>Translated_265_residues
MKIIIMKINRLSIVIMAFFCSSLLLSGCSKREKISALQQLNGKEFAIPTGTVADQLVLSKLPNAKFKYFNSVMDAALAVK
SGKADAAAYDEPILKNIAAKNSGLVVLSDMITVDNYGFAVRKDDTELKKNIDTVVLDLKNNGTTGQMMKRWFPVSGNPAP
MPVISSTGSKGILRLGTASVTEPFSFVDGSGEIVGYDIELARYIAKKLDKKLVIVNMDFGGMIPALMSSKVDMIAACITI
TDERSRQVLFSEPYYIGGIAALVRQ
>Mature_265_residues
MKIIIMKINRLSIVIMAFFCSSLLLSGCSKREKISALQQLNGKEFAIPTGTVADQLVLSKLPNAKFKYFNSVMDAALAVK
SGKADAAAYDEPILKNIAAKNSGLVVLSDMITVDNYGFAVRKDDTELKKNIDTVVLDLKNNGTTGQMMKRWFPVSGNPAP
MPVISSTGSKGILRLGTASVTEPFSFVDGSGEIVGYDIELARYIAKKLDKKLVIVNMDFGGMIPALMSSKVDMIAACITI
TDERSRQVLFSEPYYIGGIAALVRQ

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=92, Percent_Identity=36.9565217391304, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787031, Length=101, Percent_Identity=39.6039603960396, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788228, Length=107, Percent_Identity=32.7102803738318, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788649, Length=83, Percent_Identity=37.3493975903614, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787085, Length=83, Percent_Identity=36.144578313253, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788648, Length=83, Percent_Identity=31.3253012048193, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28748; Mature: 28748

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIIMKINRLSIVIMAFFCSSLLLSGCSKREKISALQQLNGKEFAIPTGTVADQLVLSK
CEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHH
LPNAKFKYFNSVMDAALAVKSGKADAAAYDEPILKNIAAKNSGLVVLSDMITVDNYGFAV
CCCHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHCCCCCEEEEEEEEEECCCCEEE
RKDDTELKKNIDTVVLDLKNNGTTGQMMKRWFPVSGNPAPMPVISSTGSKGILRLGTASV
ECCCHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCEEECCCCCCEEEECCCCC
TEPFSFVDGSGEIVGYDIELARYIAKKLDKKLVIVNMDFGGMIPALMSSKVDMIAACITI
CCCCCEECCCCCEEEEHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCHHHHEEEEEE
TDERSRQVLFSEPYYIGGIAALVRQ
ECCCCCEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure
MKIIIMKINRLSIVIMAFFCSSLLLSGCSKREKISALQQLNGKEFAIPTGTVADQLVLSK
CEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHH
LPNAKFKYFNSVMDAALAVKSGKADAAAYDEPILKNIAAKNSGLVVLSDMITVDNYGFAV
CCCHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHCCCCCEEEEEEEEEECCCCEEE
RKDDTELKKNIDTVVLDLKNNGTTGQMMKRWFPVSGNPAPMPVISSTGSKGILRLGTASV
ECCCHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCEEECCCCCCEEEECCCCC
TEPFSFVDGSGEIVGYDIELARYIAKKLDKKLVIVNMDFGGMIPALMSSKVDMIAACITI
CCCCCEECCCCCEEEEHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCHHHHEEEEEE
TDERSRQVLFSEPYYIGGIAALVRQ
ECCCCCEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9192026; 9395059; 10688204 [H]