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The map label for this gene is cobS [H]

Identifier: 119356827

GI number: 119356827

Start: 1138626

End: 1139372

Strand: Direct

Name: cobS [H]

Synonym: Cpha266_1000

Alternate gene names: 119356827

Gene position: 1138626-1139372 (Clockwise)

Preceding gene: 119356826

Following gene: 119356828

Centisome position: 36.33

GC content: 53.95

Gene sequence:

>747_bases
ATGATGCCTCGCGGTTTGGTTACAGCATTGAGAACGCTCTCGATTCTGCCTGTGCCCGGGCGGGATGCGGAACGTTTCAG
TGATTCGCTTTACTGGTTTCCGTTTGCGGGTTTTCTGCTTGGAGTCCTGCAGGCAGCTCTTGGGTATACAGGGAACACCT
TCGGATGGCCTGAACTTTCGGCTCTGCTCGTCGTGCTTGGCGGTATTGCGTTGACTCGCGGGCTTCATGCCGATGGGCTT
GCCGACTTTGCCGATGGTTTCTGGGGCGGGAAAACCAGAGAAGCCGCCCTCAGAATCATGAAAGATCCTCAAGTCGGTTC
ATTCGGGGTGCTTGCCCTTGTTGCCGTTATGGGGATGAAGTGGGTACTTCTTCTGCAACTGTTGCATCATGACGCCTTCA
ACTGGATTGTTGCCGGCATCATGCTCTCCCGGTGGATTCAGGTGCCTCTTGCCGTTCTGCTTCCGTACGCCCGAAAGGAA
GGGGGTACTGCGGCATCGTTTGTTGAAGGTTCAAAGCGAGAGCATCTTGTTTTTGCAACCATTATCATGTTTCTGCTGCT
TTTTTCGATCATGGGTTTTCAGCCTTTTCTCGCACTTCTGGCGGGAGTAGCCACTCTTCTGGCTGGTACGATTACAGGTT
TTGCTGCAAACAGGAAACTCGGCGGGGTTACCGGTGATGTTCTTGGCGCAGCAAGCGAACTGACCGAAGTGGCCGTCTGG
TTTGCCGGCGCTCTCTTTTTTCGCTGA

Upstream 100 bases:

>100_bases
GCTCTTGCCATGCGGATTATCGAGGCTTCTGTGAACGTGTATAATGAGATGCAGACCTTCAGCTCTGCCGGTGTAAGCGG
AAAAACAGAAGAGTAACATC

Downstream 100 bases:

>100_bases
CCTATTGCGCTGCCATCGTTGTTTTTTTCCTTACCTGCGCTCTGAAAGGGAGCTACTCCTGTTATTTTTTTGTTTTTTTA
GCTGGAAGCTTGTTTAATTA

Product: cobalamin-5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MMPRGLVTALRTLSILPVPGRDAERFSDSLYWFPFAGFLLGVLQAALGYTGNTFGWPELSALLVVLGGIALTRGLHADGL
ADFADGFWGGKTREAALRIMKDPQVGSFGVLALVAVMGMKWVLLLQLLHHDAFNWIVAGIMLSRWIQVPLAVLLPYARKE
GGTAASFVEGSKREHLVFATIIMFLLLFSIMGFQPFLALLAGVATLLAGTITGFAANRKLGGVTGDVLGAASELTEVAVW
FAGALFFR

Sequences:

>Translated_248_residues
MMPRGLVTALRTLSILPVPGRDAERFSDSLYWFPFAGFLLGVLQAALGYTGNTFGWPELSALLVVLGGIALTRGLHADGL
ADFADGFWGGKTREAALRIMKDPQVGSFGVLALVAVMGMKWVLLLQLLHHDAFNWIVAGIMLSRWIQVPLAVLLPYARKE
GGTAASFVEGSKREHLVFATIIMFLLLFSIMGFQPFLALLAGVATLLAGTITGFAANRKLGGVTGDVLGAASELTEVAVW
FAGALFFR
>Mature_248_residues
MMPRGLVTALRTLSILPVPGRDAERFSDSLYWFPFAGFLLGVLQAALGYTGNTFGWPELSALLVVLGGIALTRGLHADGL
ADFADGFWGGKTREAALRIMKDPQVGSFGVLALVAVMGMKWVLLLQLLHHDAFNWIVAGIMLSRWIQVPLAVLLPYARKE
GGTAASFVEGSKREHLVFATIIMFLLLFSIMGFQPFLALLAGVATLLAGTITGFAANRKLGGVTGDVLGAASELTEVAVW
FAGALFFR

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=237, Percent_Identity=34.1772151898734, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26585; Mature: 26585

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00151 ACYLPHOSPHATASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPRGLVTALRTLSILPVPGRDAERFSDSLYWFPFAGFLLGVLQAALGYTGNTFGWPELS
CCCHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
ALLVVLGGIALTRGLHADGLADFADGFWGGKTREAALRIMKDPQVGSFGVLALVAVMGMK
HHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
WVLLLQLLHHDAFNWIVAGIMLSRWIQVPLAVLLPYARKEGGTAASFVEGSKREHLVFAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHH
IIMFLLLFSIMGFQPFLALLAGVATLLAGTITGFAANRKLGGVTGDVLGAASELTEVAVW
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
FAGALFFR
HHHHHHCC
>Mature Secondary Structure
MMPRGLVTALRTLSILPVPGRDAERFSDSLYWFPFAGFLLGVLQAALGYTGNTFGWPELS
CCCHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
ALLVVLGGIALTRGLHADGLADFADGFWGGKTREAALRIMKDPQVGSFGVLALVAVMGMK
HHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
WVLLLQLLHHDAFNWIVAGIMLSRWIQVPLAVLLPYARKEGGTAASFVEGSKREHLVFAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHH
IIMFLLLFSIMGFQPFLALLAGVATLLAGTITGFAANRKLGGVTGDVLGAASELTEVAVW
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
FAGALFFR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA