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The map label for this gene is hupB [H]

Identifier: 119356801

GI number: 119356801

Start: 1115404

End: 1117122

Strand: Direct

Name: hupB [H]

Synonym: Cpha266_0973

Alternate gene names: 119356801

Gene position: 1115404-1117122 (Clockwise)

Preceding gene: 119356800

Following gene: 119356802

Centisome position: 35.59

GC content: 48.52

Gene sequence:

>1719_bases
ATGTCAAGAAAAATTGTTGTTGATCCTATTCCCCGTATAGAGGGACACCTGAGAATTGAGGCAAAGCTGAATGAGAGCAA
TCAGATTGAAGAGGCGTTCAGCAGCGGTACCATGTGGCGGGGTATCGAGGTTATACTCAAAGGTCGAGATCCCCGAGATG
CGTGGGCTTTTGCTGAACGTATCTGTGGTGTCTGTACCAGTGTTCATGCGCTTGCATCGGTCCGTTGTGTCGAGGATGCT
CTGGGTATTGATGTGCCTCTTAATGCCCGCATTATCAGAAACCTGATGAATGCCACGCAGCAGACACAGGATCATCTGGT
TCACTTTTATCATCTTCACGCACTTGACTGGGTCGATGTTGTCAGTGCGTTGAAGGCGGATCCGAAAATGACATCCGATA
TTGCTCAGAGCATCTCTCCGTGGCCGAAATCATCAGTGGGGTATTTCAAGGATCTTCAGCAGCGTCTTACAGGTTTTGTC
GAGAGCGGTCAACTCGGCATATTTTCAAATGGTTACTGGGGACATCCTGCCTACAGGCTGCCTCCTGAAGTCAACCTTCT
TGCGGTTGCGCACTACCTTGAAGCTCTTGATTTTCAGAAGGAGATCATCAAGATCCATACCATTTTCGGAGGCAAGAATC
CGCATCCGAACTATCTGGTTGGAGGAATGGCGTGCGCTATCGATCCAAACAGTGATACGGCAATCAATATCGAGCGTTTT
GCCTTGATCAAGAAGATCATCGACGATACCAATACCTTTATTGATCAGGTCTATATTCCGGACCTGATCGCTATTGCCGG
TTATTACAAGGATTGGCTCTATGGCGGCGGTCTGGGCAACTATCTCAGTTATGGCGATTTTCCGGAAAATTCACTTGATG
ACTACAAGACCCTGCTTTGGCCGAGAGGAGCCATTCTCAACAGGGATCTTTCAAACGTTATCGATGTTGATCCACGCGAT
TCCGCCCAGGTTACCGAAGAGGTCAGCCATAGCTGGTATACCTATTCAAAAGGGGACAGTAAAGGCCTCCATCCCTGGGA
GGGTGAGACAAAACCGGCTTATACCGGCCCTAAACCACCGTTTGAATTTCTCGATACTGACAAGAAGTACAGTTGGTTAA
AAACTCCCCGGTGGAAAAACCACGCAATGGAGGTCGGACCGCTTGCCCGGGTTCTTGTGGCATATGCAAAAGGGGATCCC
ATGATCAAGGATACCGTCGGTATGGTTCTCGGCAAGCTTCAGGTTGGTCCGGAAGCACTGTTTTCGACTCTTGGCAGAAC
TGCTGCACGGGGTATCGAGTGCAAGCAGACTGCCGGATTCATGCGTCATTTTTATGATCAGCTTGTTGATAATGTCAAAA
AAGGTGATTACAGGACGTTCAACAGCGATAAATGGGATCCGTCAACATGGCCTTCTGAGTCGAAGGGCTTCGGATATACT
GAAGCTCCGCGTGGTTCGCTTGGTCACTGGATACATATCAAAGACCAGAAGATCAAGGAGTACCAGATTGTTGTCCCTTC
AACATGGAACGCTTCGCCCAGAGATGCCGCAGGTAATTCAGGCGCTTATGAAGCTGCATTGAAAGGAACTCCCATGGCTA
ATCCTGAACAGCCGCTTGAGATTATCAGAACTGTACACTCATTCGATCCCTGTCTTGCATGCGCATCCCATGTGGTTGAT
ATGCATGGCAAAGAGATCACCAAGGTCAAAATCGTTTAA

Upstream 100 bases:

>100_bases
CTGCGGCGGCCGGAGCTGCGGCACATGCGACGATTACGGCAATTAAAAAGGGAAAATCAGGTAAAGGTAATGACAAAGCT
TAAAGAAGGAGTGATTCGTT

Downstream 100 bases:

>100_bases
ACGGAGCAGATCATGGGTAGAATTATTGAAGAAATCTATGTCTGGAGATTGCCTGTAAGGTTCTATCATTGGGTGAATGC
CCTCTGCACGGTTATTCTTC

Product: nickel-dependent hydrogenase, large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MSRKIVVDPIPRIEGHLRIEAKLNESNQIEEAFSSGTMWRGIEVILKGRDPRDAWAFAERICGVCTSVHALASVRCVEDA
LGIDVPLNARIIRNLMNATQQTQDHLVHFYHLHALDWVDVVSALKADPKMTSDIAQSISPWPKSSVGYFKDLQQRLTGFV
ESGQLGIFSNGYWGHPAYRLPPEVNLLAVAHYLEALDFQKEIIKIHTIFGGKNPHPNYLVGGMACAIDPNSDTAINIERF
ALIKKIIDDTNTFIDQVYIPDLIAIAGYYKDWLYGGGLGNYLSYGDFPENSLDDYKTLLWPRGAILNRDLSNVIDVDPRD
SAQVTEEVSHSWYTYSKGDSKGLHPWEGETKPAYTGPKPPFEFLDTDKKYSWLKTPRWKNHAMEVGPLARVLVAYAKGDP
MIKDTVGMVLGKLQVGPEALFSTLGRTAARGIECKQTAGFMRHFYDQLVDNVKKGDYRTFNSDKWDPSTWPSESKGFGYT
EAPRGSLGHWIHIKDQKIKEYQIVVPSTWNASPRDAAGNSGAYEAALKGTPMANPEQPLEIIRTVHSFDPCLACASHVVD
MHGKEITKVKIV

Sequences:

>Translated_572_residues
MSRKIVVDPIPRIEGHLRIEAKLNESNQIEEAFSSGTMWRGIEVILKGRDPRDAWAFAERICGVCTSVHALASVRCVEDA
LGIDVPLNARIIRNLMNATQQTQDHLVHFYHLHALDWVDVVSALKADPKMTSDIAQSISPWPKSSVGYFKDLQQRLTGFV
ESGQLGIFSNGYWGHPAYRLPPEVNLLAVAHYLEALDFQKEIIKIHTIFGGKNPHPNYLVGGMACAIDPNSDTAINIERF
ALIKKIIDDTNTFIDQVYIPDLIAIAGYYKDWLYGGGLGNYLSYGDFPENSLDDYKTLLWPRGAILNRDLSNVIDVDPRD
SAQVTEEVSHSWYTYSKGDSKGLHPWEGETKPAYTGPKPPFEFLDTDKKYSWLKTPRWKNHAMEVGPLARVLVAYAKGDP
MIKDTVGMVLGKLQVGPEALFSTLGRTAARGIECKQTAGFMRHFYDQLVDNVKKGDYRTFNSDKWDPSTWPSESKGFGYT
EAPRGSLGHWIHIKDQKIKEYQIVVPSTWNASPRDAAGNSGAYEAALKGTPMANPEQPLEIIRTVHSFDPCLACASHVVD
MHGKEITKVKIV
>Mature_571_residues
SRKIVVDPIPRIEGHLRIEAKLNESNQIEEAFSSGTMWRGIEVILKGRDPRDAWAFAERICGVCTSVHALASVRCVEDAL
GIDVPLNARIIRNLMNATQQTQDHLVHFYHLHALDWVDVVSALKADPKMTSDIAQSISPWPKSSVGYFKDLQQRLTGFVE
SGQLGIFSNGYWGHPAYRLPPEVNLLAVAHYLEALDFQKEIIKIHTIFGGKNPHPNYLVGGMACAIDPNSDTAINIERFA
LIKKIIDDTNTFIDQVYIPDLIAIAGYYKDWLYGGGLGNYLSYGDFPENSLDDYKTLLWPRGAILNRDLSNVIDVDPRDS
AQVTEEVSHSWYTYSKGDSKGLHPWEGETKPAYTGPKPPFEFLDTDKKYSWLKTPRWKNHAMEVGPLARVLVAYAKGDPM
IKDTVGMVLGKLQVGPEALFSTLGRTAARGIECKQTAGFMRHFYDQLVDNVKKGDYRTFNSDKWDPSTWPSESKGFGYTE
APRGSLGHWIHIKDQKIKEYQIVVPSTWNASPRDAAGNSGAYEAALKGTPMANPEQPLEIIRTVHSFDPCLACASHVVDM
HGKEITKVKIV

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=581, Percent_Identity=56.1101549053356, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI1789368, Length=584, Percent_Identity=46.4041095890411, Blast_Score=520, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 63975; Mature: 63844

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKIVVDPIPRIEGHLRIEAKLNESNQIEEAFSSGTMWRGIEVILKGRDPRDAWAFAER
CCCCEEECCCCCCCCCEEEEEECCCCHHHHHHHHCCCHHCCEEEEEECCCCHHHHHHHHH
ICGVCTSVHALASVRCVEDALGIDVPLNARIIRNLMNATQQTQDHLVHFYHLHALDWVDV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSALKADPKMTSDIAQSISPWPKSSVGYFKDLQQRLTGFVESGQLGIFSNGYWGHPAYRL
HHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
PPEVNLLAVAHYLEALDFQKEIIKIHTIFGGKNPHPNYLVGGMACAIDPNSDTAINIERF
CCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEECCEEEEECCCCCCEEEHHHH
ALIKKIIDDTNTFIDQVYIPDLIAIAGYYKDWLYGGGLGNYLSYGDFPENSLDDYKTLLW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHC
PRGAILNRDLSNVIDVDPRDSAQVTEEVSHSWYTYSKGDSKGLHPWEGETKPAYTGPKPP
CCCHHHCCCHHHHCCCCCCCHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCC
FEFLDTDKKYSWLKTPRWKNHAMEVGPLARVLVAYAKGDPMIKDTVGMVLGKLQVGPEAL
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHH
FSTLGRTAARGIECKQTAGFMRHFYDQLVDNVKKGDYRTFNSDKWDPSTWPSESKGFGYT
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EAPRGSLGHWIHIKDQKIKEYQIVVPSTWNASPRDAAGNSGAYEAALKGTPMANPEQPLE
CCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCEEHHCCCCCCCCCCHHHH
IIRTVHSFDPCLACASHVVDMHGKEITKVKIV
HHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure 
SRKIVVDPIPRIEGHLRIEAKLNESNQIEEAFSSGTMWRGIEVILKGRDPRDAWAFAER
CCCEEECCCCCCCCCEEEEEECCCCHHHHHHHHCCCHHCCEEEEEECCCCHHHHHHHHH
ICGVCTSVHALASVRCVEDALGIDVPLNARIIRNLMNATQQTQDHLVHFYHLHALDWVDV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSALKADPKMTSDIAQSISPWPKSSVGYFKDLQQRLTGFVESGQLGIFSNGYWGHPAYRL
HHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
PPEVNLLAVAHYLEALDFQKEIIKIHTIFGGKNPHPNYLVGGMACAIDPNSDTAINIERF
CCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEEECCEEEEECCCCCCEEEHHHH
ALIKKIIDDTNTFIDQVYIPDLIAIAGYYKDWLYGGGLGNYLSYGDFPENSLDDYKTLLW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHC
PRGAILNRDLSNVIDVDPRDSAQVTEEVSHSWYTYSKGDSKGLHPWEGETKPAYTGPKPP
CCCHHHCCCHHHHCCCCCCCHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCC
FEFLDTDKKYSWLKTPRWKNHAMEVGPLARVLVAYAKGDPMIKDTVGMVLGKLQVGPEAL
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHH
FSTLGRTAARGIECKQTAGFMRHFYDQLVDNVKKGDYRTFNSDKWDPSTWPSESKGFGYT
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EAPRGSLGHWIHIKDQKIKEYQIVVPSTWNASPRDAAGNSGAYEAALKGTPMANPEQPLE
CCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCEEHHCCCCCCCCCCHHHH
IIRTVHSFDPCLACASHVVDMHGKEITKVKIV
HHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3054886; 12597275; 8163174 [H]