Click here to switch to the map view.

The map label for this gene is amtB [C]

Identifier: 118578925

GI number: 118578925

Start: 507168

End: 508619

Strand: Direct

Name: amtB [C]

Synonym: Ppro_0486

Alternate gene names: 118578925

Gene position: 507168-508619 (Clockwise)

Preceding gene: 118578924

Following gene: 118578926

Centisome position: 12.65

GC content: 59.57

Gene sequence:

>1452_bases
ATGAAACTCAAATTATTCCTAAGTATAAGCATGCTCATGCTTATACTGGTTGCCGGCAGGATGCCGGTTGTCGCACACGC
CGAAGAAAACAAGGATACTGCTTTCGCCGTGACTGCCCCGGCAGCGGCAAGCGTGGCCGTCGCCGAGACCGCTCCGGCTG
CTGCCACTGAAGCCGCGCCTGCCCCGAAGCCGGTGACCGCCGAAGATGTCCAGACCAATCTGAACTTTGTCTGGACACTG
ATTGCGGCCTTTCTGGTCTTTATCATGCAGGCCGGTTTCGCGCTGGTGGAAATCGGCTTTACCCGGGCCAAGAACGCCTG
CAACATCCTGATGAAGAACATGATGGACTTCTCCGCCGGAGCGATCGCCTACTGGGCGGTAGGATTCGGGCTCATGTTCG
GTGTCAGCAACGGCTTCTTCGGAACCACGCACTTCTTCTTCAACGGCGTTGCCGAGGCTGATCAGCCGTGGAACTTCGCC
TTCTGGATGTTCCAGGTTGTCTTTGCCGCCACCACCGCCACGATTATCTCCGGAGCTGTGGCTGAACGCACCAAGTTCAG
CGCCTACATCGTCTACTCCTTCGTCGTATCGGCCGTGATCTACCCCATCTTCGGCTCCTGGGCCTGGGGCAATCTCTACA
AGGGGACCGGCTGGCTGGCAAGCATGGGCTTCATCGATTTCGCCGGTTCGACGGTCGTTCACTCCATGGGGGGCTGGCTC
TCCCTTGCCGGTGCCATCATCGTCGGTCCGCGCATCGGCAAGTACGACAAAAATGGCAACCTGAAGCCGATTCCCGGACA
CAATATACCCTTGGCTGGCCTGGGTGTCTTCCTGCTCTGGTTCGGCTGGTACGGCTTCAACCCCGGTTCCACTACCACCG
GCACTACCGGCATCGCCATCATCGCGGTGACGACGACCCTGGCTGCTGCAGCTGGCGCCAACTCGGCCATGTTCTTTACC
TGGCTCAAGTTCGGTAAACCGGATGTGGGCATGACCCTGAACGGGGCCCTGGCTGGCTTGGTGGGTGTCACCGCCGGCTG
CGCCAACGTCTCTCCCCTCTCCGCAGTGATCATCGGAATGGTGGCTGGCGTGCTGGTGGTTCTGTCGGTCATGTTCTTCG
ACAGAATCAAGATCGATGACCCGGTCGGCGCGGTGTCGGTGCATGGCGTTTGTGGCGCCTGGGGGACCTTGGCGGCCGGA
CTCTTCAACAGCGCCGGTTTCAGCCTGAAACAGGTCGGTGTCCAGCTGGTCGGAATGGGCGCCTGCTTCGTCTGGGCTTT
TGGATGCGGCCTGATCCTCTTCAAGCTGATCGACATGGTCATCGGCATGCGGGTCTCCAGGGAAGAGGAAATGGCCGGAC
TGGATCTGTGCGAACACGGCGCATCGGCTTACCCCGACTTCCAGACGGTTCACTTCGGCGCAATGTTCAACCCGGACGAA
GGAAAGAAATAA

Upstream 100 bases:

>100_bases
GCTGGAGGATGCGGTCAGAATCAGAACCGGCGAGAGTGGTGGAGACGCCATTTAACGCTGCACACACGGGTGCACTGGAA
AACCCGAGGGAGGCTTCGCT

Downstream 100 bases:

>100_bases
TCTGTTCCTGCACGAGGGGCCGGCCCCATCGCCATGATGGTGGCCGGACTCTCGTTTTCCGCACCCACACACCACTTATC
GAAGGAGATACTGCATGAAA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKLKLFLSISMLMLILVAGRMPVVAHAEENKDTAFAVTAPAAASVAVAETAPAAATEAAPAPKPVTAEDVQTNLNFVWTL
IAAFLVFIMQAGFALVEIGFTRAKNACNILMKNMMDFSAGAIAYWAVGFGLMFGVSNGFFGTTHFFFNGVAEADQPWNFA
FWMFQVVFAATTATIISGAVAERTKFSAYIVYSFVVSAVIYPIFGSWAWGNLYKGTGWLASMGFIDFAGSTVVHSMGGWL
SLAGAIIVGPRIGKYDKNGNLKPIPGHNIPLAGLGVFLLWFGWYGFNPGSTTTGTTGIAIIAVTTTLAAAAGANSAMFFT
WLKFGKPDVGMTLNGALAGLVGVTAGCANVSPLSAVIIGMVAGVLVVLSVMFFDRIKIDDPVGAVSVHGVCGAWGTLAAG
LFNSAGFSLKQVGVQLVGMGACFVWAFGCGLILFKLIDMVIGMRVSREEEMAGLDLCEHGASAYPDFQTVHFGAMFNPDE
GKK

Sequences:

>Translated_483_residues
MKLKLFLSISMLMLILVAGRMPVVAHAEENKDTAFAVTAPAAASVAVAETAPAAATEAAPAPKPVTAEDVQTNLNFVWTL
IAAFLVFIMQAGFALVEIGFTRAKNACNILMKNMMDFSAGAIAYWAVGFGLMFGVSNGFFGTTHFFFNGVAEADQPWNFA
FWMFQVVFAATTATIISGAVAERTKFSAYIVYSFVVSAVIYPIFGSWAWGNLYKGTGWLASMGFIDFAGSTVVHSMGGWL
SLAGAIIVGPRIGKYDKNGNLKPIPGHNIPLAGLGVFLLWFGWYGFNPGSTTTGTTGIAIIAVTTTLAAAAGANSAMFFT
WLKFGKPDVGMTLNGALAGLVGVTAGCANVSPLSAVIIGMVAGVLVVLSVMFFDRIKIDDPVGAVSVHGVCGAWGTLAAG
LFNSAGFSLKQVGVQLVGMGACFVWAFGCGLILFKLIDMVIGMRVSREEEMAGLDLCEHGASAYPDFQTVHFGAMFNPDE
GKK
>Mature_483_residues
MKLKLFLSISMLMLILVAGRMPVVAHAEENKDTAFAVTAPAAASVAVAETAPAAATEAAPAPKPVTAEDVQTNLNFVWTL
IAAFLVFIMQAGFALVEIGFTRAKNACNILMKNMMDFSAGAIAYWAVGFGLMFGVSNGFFGTTHFFFNGVAEADQPWNFA
FWMFQVVFAATTATIISGAVAERTKFSAYIVYSFVVSAVIYPIFGSWAWGNLYKGTGWLASMGFIDFAGSTVVHSMGGWL
SLAGAIIVGPRIGKYDKNGNLKPIPGHNIPLAGLGVFLLWFGWYGFNPGSTTTGTTGIAIIAVTTTLAAAAGANSAMFFT
WLKFGKPDVGMTLNGALAGLVGVTAGCANVSPLSAVIIGMVAGVLVVLSVMFFDRIKIDDPVGAVSVHGVCGAWGTLAAG
LFNSAGFSLKQVGVQLVGMGACFVWAFGCGLILFKLIDMVIGMRVSREEEMAGLDLCEHGASAYPDFQTVHFGAMFNPDE
GKK

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=416, Percent_Identity=34.375, Blast_Score=193, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17550180, Length=464, Percent_Identity=34.4827586206897, Blast_Score=234, Evalue=9e-62,
Organism=Caenorhabditis elegans, GI17550182, Length=438, Percent_Identity=35.6164383561644, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI32564042, Length=416, Percent_Identity=33.6538461538462, Blast_Score=218, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI133931100, Length=426, Percent_Identity=30.9859154929577, Blast_Score=179, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6324187, Length=433, Percent_Identity=30.9468822170901, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321559, Length=432, Percent_Identity=25.2314814814815, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6325396, Length=424, Percent_Identity=25, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161078314, Length=425, Percent_Identity=36.4705882352941, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 50789; Mature: 50789

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKLFLSISMLMLILVAGRMPVVAHAEENKDTAFAVTAPAAASVAVAETAPAAATEAAP
CEEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCC
APKPVTAEDVQTNLNFVWTLIAAFLVFIMQAGFALVEIGFTRAKNACNILMKNMMDFSAG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECHHHHHHHHHHHHHHHHHHCCC
AIAYWAVGFGLMFGVSNGFFGTTHFFFNGVAEADQPWNFAFWMFQVVFAATTATIISGAV
HHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AERTKFSAYIVYSFVVSAVIYPIFGSWAWGNLYKGTGWLASMGFIDFAGSTVVHSMGGWL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHCCHHHHHHHCCHH
SLAGAIIVGPRIGKYDKNGNLKPIPGHNIPLAGLGVFLLWFGWYGFNPGSTTTGTTGIAI
HHCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
IAVTTTLAAAAGANSAMFFTWLKFGKPDVGMTLNGALAGLVGVTAGCANVSPLSAVIIGM
EEHHHHHHHHCCCCCCEEEEHHHCCCCCCCEEECHHHHHHHHHHCCCCCCCHHHHHHHHH
VAGVLVVLSVMFFDRIKIDDPVGAVSVHGVCGAWGTLAAGLFNSAGFSLKQVGVQLVGMG
HHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHH
ACFVWAFGCGLILFKLIDMVIGMRVSREEEMAGLDLCEHGASAYPDFQTVHFGAMFNPDE
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCC
GKK
CCC
>Mature Secondary Structure
MKLKLFLSISMLMLILVAGRMPVVAHAEENKDTAFAVTAPAAASVAVAETAPAAATEAAP
CEEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCC
APKPVTAEDVQTNLNFVWTLIAAFLVFIMQAGFALVEIGFTRAKNACNILMKNMMDFSAG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECHHHHHHHHHHHHHHHHHHCCC
AIAYWAVGFGLMFGVSNGFFGTTHFFFNGVAEADQPWNFAFWMFQVVFAATTATIISGAV
HHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AERTKFSAYIVYSFVVSAVIYPIFGSWAWGNLYKGTGWLASMGFIDFAGSTVVHSMGGWL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHCCHHHHHHHCCHH
SLAGAIIVGPRIGKYDKNGNLKPIPGHNIPLAGLGVFLLWFGWYGFNPGSTTTGTTGIAI
HHCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
IAVTTTLAAAAGANSAMFFTWLKFGKPDVGMTLNGALAGLVGVTAGCANVSPLSAVIIGM
EEHHHHHHHHCCCCCCEEEEHHHCCCCCCCEEECHHHHHHHHHHCCCCCCCHHHHHHHHH
VAGVLVVLSVMFFDRIKIDDPVGAVSVHGVCGAWGTLAAGLFNSAGFSLKQVGVQLVGMG
HHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHH
ACFVWAFGCGLILFKLIDMVIGMRVSREEEMAGLDLCEHGASAYPDFQTVHFGAMFNPDE
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCC
GKK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]