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The map label for this gene is hsdM [H]

Identifier: 118578795

GI number: 118578795

Start: 357732

End: 359198

Strand: Direct

Name: hsdM [H]

Synonym: Ppro_0353

Alternate gene names: 118578795

Gene position: 357732-359198 (Clockwise)

Preceding gene: 118578794

Following gene: 118578796

Centisome position: 8.93

GC content: 61.28

Gene sequence:

>1467_bases
ATGTCCCTAACCACCCTGATCAAAGCCATCCAGGACATTATGCGCAAGGATGTGGGCGTTGATGGCGACGCCCAACGCAT
CAGCCAGATGGTCTGGCTGATGTTCCTGAAGATCTTTGACGACAAGGAACAGGAGTGGCAGCTCACCGTTCCCGGCTACA
AATCGCCGCTGCCCAGCCGTTTCCGCTGGTCCAGTTGGGCCAAGAATCCCGAGGGGATGACCGGCGAGGAGCTGATCGAC
TTCGTCAACAACGACCTCTTCCCGGCGCTCAAGAAGCTGGCCACCGCCGCCGGTGTGTCGCCCCATGGCAAGGTGGTCGG
CTCGGTGTTCGAGGACGCCTACAACTACATGAAGTCCGGTACCCTGCTGCGCCAGGTGATCAACACCATCGAAGAGGATG
TGGACTTCAATAAATCCGGCGACCGTCACCTGTTCAACGACATCTACGAAAAGATCCTCTCCGACCTGCAATCGGCCGGG
AACGCGGGCGAATACTACACCCCCCGCGCCGTCACCCGGTTCATGGTCGACATGCTCGATCCCCAGCTCGGGCAGACCAT
CCTCGACCCGGCCTGCGGCACCGGCGGCTTTCTCACCTGCGCCATCGAGCACCTGAACCATCAGGTCAAGACCGCCGCCG
ACCGCACGCGACTGCAGGAGTGTATCTTTGGCGTAGAGAAGAAACCGCTGCCGCACATGCTGGCCATGACCAACATGATG
CTGCACGGTATCGACGTGCCCACCAACGTCCGCCACGACAACACCCTCTCCCGGCCGCTCAAGGATTACGGCCCGAAAGA
CCGGGTGGACCTGATCATCACCAACCCCCCCTTCGGCGGCATGGAAGAGGACGGCATCGAGAACAACTTCCCGCGCAAGT
ACCAGACCCGCGAGACCGCCGACCTCTTCATGGCGCTGATCATGCACCTCTTGAAACACGACACCGGCAAGGCGGCCGTG
GTGCTGCCCGACGGCTTTCTCTTCGGCGAAGGCACCAAGACCAACCTCAAGCGCGAGCTGCTGGAGGAGTTCAACCTGCA
CACCATCGTGCGCCTGCCCAAAGGCGTGTTCAGCCCCTACACCAGCATCGCCACCAACATCCTCTTCTTCGAGAAAGGCG
GCCCGACCAGGGAGGTCTGGTTTTTCGAGCACCCCTATCCAGAGGGGTACAAGTCCTACTCCCGCTCCAAGCCGTTGACC
ATCGCCGAGTTCGATCTGGAAAAAGCCTGGTGGGGCGGAGCCGAGCGTCGTGGGCGCAAGACCACCGAGCAGGCCTGGAA
AGTCTCGGCCAAGGTGCTGGCCGAGCGCACCTACAACCTGGACTGCAAGAACCCCCACGAGGTGGAGGTCAACCACCGCG
ATCCGGAAGAGTTGATGGCTGAATATCAGGCTATCGTCCACCAGCTCCAAGCGGCGCAGGCAGCCTTGAAAGCCGAGTTG
ATGGCCTGCCTGGGGGGCAAGGCATGA

Upstream 100 bases:

>100_bases
CTTCGCGCCACCCCTCCAGAGGAGGGGAATCTTGGGCCTCAACTTGACTGATTCAAACAGGACTTCGTGCCTTCGTGTGA
ACCGATAAAGGAATCAAATC

Downstream 100 bases:

>100_bases
GCAGGAAGAAAAAGCCTGGCAAGGAAGTCTCCATCGTTCGCTCCTCGGCCGCCGAATACCTCACCTTTGTCGCCGCCAGC
GGTGCGGGCGGCGTCGAGGC

Product: N-6 DNA methylase

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSLTTLIKAIQDIMRKDVGVDGDAQRISQMVWLMFLKIFDDKEQEWQLTVPGYKSPLPSRFRWSSWAKNPEGMTGEELID
FVNNDLFPALKKLATAAGVSPHGKVVGSVFEDAYNYMKSGTLLRQVINTIEEDVDFNKSGDRHLFNDIYEKILSDLQSAG
NAGEYYTPRAVTRFMVDMLDPQLGQTILDPACGTGGFLTCAIEHLNHQVKTAADRTRLQECIFGVEKKPLPHMLAMTNMM
LHGIDVPTNVRHDNTLSRPLKDYGPKDRVDLIITNPPFGGMEEDGIENNFPRKYQTRETADLFMALIMHLLKHDTGKAAV
VLPDGFLFGEGTKTNLKRELLEEFNLHTIVRLPKGVFSPYTSIATNILFFEKGGPTREVWFFEHPYPEGYKSYSRSKPLT
IAEFDLEKAWWGGAERRGRKTTEQAWKVSAKVLAERTYNLDCKNPHEVEVNHRDPEELMAEYQAIVHQLQAAQAALKAEL
MACLGGKA

Sequences:

>Translated_488_residues
MSLTTLIKAIQDIMRKDVGVDGDAQRISQMVWLMFLKIFDDKEQEWQLTVPGYKSPLPSRFRWSSWAKNPEGMTGEELID
FVNNDLFPALKKLATAAGVSPHGKVVGSVFEDAYNYMKSGTLLRQVINTIEEDVDFNKSGDRHLFNDIYEKILSDLQSAG
NAGEYYTPRAVTRFMVDMLDPQLGQTILDPACGTGGFLTCAIEHLNHQVKTAADRTRLQECIFGVEKKPLPHMLAMTNMM
LHGIDVPTNVRHDNTLSRPLKDYGPKDRVDLIITNPPFGGMEEDGIENNFPRKYQTRETADLFMALIMHLLKHDTGKAAV
VLPDGFLFGEGTKTNLKRELLEEFNLHTIVRLPKGVFSPYTSIATNILFFEKGGPTREVWFFEHPYPEGYKSYSRSKPLT
IAEFDLEKAWWGGAERRGRKTTEQAWKVSAKVLAERTYNLDCKNPHEVEVNHRDPEELMAEYQAIVHQLQAAQAALKAEL
MACLGGKA
>Mature_487_residues
SLTTLIKAIQDIMRKDVGVDGDAQRISQMVWLMFLKIFDDKEQEWQLTVPGYKSPLPSRFRWSSWAKNPEGMTGEELIDF
VNNDLFPALKKLATAAGVSPHGKVVGSVFEDAYNYMKSGTLLRQVINTIEEDVDFNKSGDRHLFNDIYEKILSDLQSAGN
AGEYYTPRAVTRFMVDMLDPQLGQTILDPACGTGGFLTCAIEHLNHQVKTAADRTRLQECIFGVEKKPLPHMLAMTNMML
HGIDVPTNVRHDNTLSRPLKDYGPKDRVDLIITNPPFGGMEEDGIENNFPRKYQTRETADLFMALIMHLLKHDTGKAAVV
LPDGFLFGEGTKTNLKRELLEEFNLHTIVRLPKGVFSPYTSIATNILFFEKGGPTREVWFFEHPYPEGYKSYSRSKPLTI
AEFDLEKAWWGGAERRGRKTTEQAWKVSAKVLAERTYNLDCKNPHEVEVNHRDPEELMAEYQAIVHQLQAAQAALKAELM
ACLGGKA

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=428, Percent_Identity=26.8691588785047, Blast_Score=135, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 55177; Mature: 55046

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTTLIKAIQDIMRKDVGVDGDAQRISQMVWLMFLKIFDDKEQEWQLTVPGYKSPLPSR
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCC
FRWSSWAKNPEGMTGEELIDFVNNDLFPALKKLATAAGVSPHGKVVGSVFEDAYNYMKSG
CCCHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
TLLRQVINTIEEDVDFNKSGDRHLFNDIYEKILSDLQSAGNAGEYYTPRAVTRFMVDMLD
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
PQLGQTILDPACGTGGFLTCAIEHLNHQVKTAADRTRLQECIFGVEKKPLPHMLAMTNMM
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LHGIDVPTNVRHDNTLSRPLKDYGPKDRVDLIITNPPFGGMEEDGIENNFPRKYQTRETA
HHCCCCCCCCCCCCHHHCCHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
DLFMALIMHLLKHDTGKAAVVLPDGFLFGEGTKTNLKRELLEEFNLHTIVRLPKGVFSPY
HHHHHHHHHHHHCCCCCEEEEECCCEEECCCCHHHHHHHHHHHCCCHHHHHCCHHHHCHH
TSIATNILFFEKGGPTREVWFFEHPYPEGYKSYSRSKPLTIAEFDLEKAWWGGAERRGRK
HHHHHHEEEEECCCCCEEEEEEECCCCHHHHHHCCCCCCEEEECCHHHHHCCCHHHCCCC
TTEQAWKVSAKVLAERTYNLDCKNPHEVEVNHRDPEELMAEYQAIVHQLQAAQAALKAEL
HHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MACLGGKA
HHHHCCCC
>Mature Secondary Structure 
SLTTLIKAIQDIMRKDVGVDGDAQRISQMVWLMFLKIFDDKEQEWQLTVPGYKSPLPSR
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCC
FRWSSWAKNPEGMTGEELIDFVNNDLFPALKKLATAAGVSPHGKVVGSVFEDAYNYMKSG
CCCHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
TLLRQVINTIEEDVDFNKSGDRHLFNDIYEKILSDLQSAGNAGEYYTPRAVTRFMVDMLD
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
PQLGQTILDPACGTGGFLTCAIEHLNHQVKTAADRTRLQECIFGVEKKPLPHMLAMTNMM
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LHGIDVPTNVRHDNTLSRPLKDYGPKDRVDLIITNPPFGGMEEDGIENNFPRKYQTRETA
HHCCCCCCCCCCCCHHHCCHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
DLFMALIMHLLKHDTGKAAVVLPDGFLFGEGTKTNLKRELLEEFNLHTIVRLPKGVFSPY
HHHHHHHHHHHHCCCCCEEEEECCCEEECCCCHHHHHHHHHHHCCCHHHHHCCHHHHCHH
TSIATNILFFEKGGPTREVWFFEHPYPEGYKSYSRSKPLTIAEFDLEKAWWGGAERRGRK
HHHHHHEEEEECCCCCEEEEEEECCCCHHHHHHCCCCCCEEEECCHHHHHCCCHHHCCCC
TTEQAWKVSAKVLAERTYNLDCKNPHEVEVNHRDPEELMAEYQAIVHQLQAAQAALKAEL
HHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MACLGGKA
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]