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The map label for this gene is mtnN [C]

Identifier: 118578744

GI number: 118578744

Start: 301339

End: 302064

Strand: Direct

Name: mtnN [C]

Synonym: Ppro_0302

Alternate gene names: 118578744

Gene position: 301339-302064 (Clockwise)

Preceding gene: 118578730

Following gene: 118578748

Centisome position: 7.52

GC content: 65.15

Gene sequence:

>726_bases
ATGAAGATAGTCATTGTAACCGCCATGCCGGAAGAGAGCGCGGCGGTGATGAAGCGGGCCGGGGGGCTGAAAAAGACGCT
GCTGGAAAGCAGAAAGAGGTACGGCTTCTCCATGTGCGGACACGAGCTGATCCTGGTTGAGTCGGGGATGGGGATGCTCA
ATGCCGGCTGGGCTGCCGCGGCCCTTGCCGCCGAAGGGGCCGACCTGATGATATCCGCCGGTTTCGGGGGCGCCGTTCTG
GAAGGGCTCGGGGTGGGGGAGCTGGTCATGGCCCGGCAGGTGCTGCACTGGAGCGGGTCCGAGTTGGAGGAGGTGGCGGT
GGGATTCTACGGCCTGAATGCCGTTGCCGACAGTCTCTCGCTGCGGCTGGCAACATTCGTTTCCTGCGATCAGATCCTCA
ACAAGCGTGACCTGGCGCTGCGTCTCCCTCCCCAGGCGACTAATCCGGTGGTGGAGATGGAGAGCGCAGCCGTGGCCCGC
GTCGCGGCCAGCCATGGCATCCCGTTCCTGGCCATGCGCGCCATCAGCGATCCCTGGGATGAGGAGATGGACTTCACCAT
CGACGAGTTCTGCGATGACAGCATGCGCATCCGGCCCACCAAGGTGCTGGCTACCATTCTGCGTCGGCCGCGTATCATCC
CGCAGTTGGTCCGCCTGGCCCGAACCAGTCGCGTGGCGGCTGGTGGACTCGCGCGGGGCACGGAGCGCCTGCTGGCAGCG
ATCTGA

Upstream 100 bases:

>100_bases
GAAACCGTTTGTCCAGCTTTTGTTGATTTGATCTTGGATCAATGATGCAAATCGGTGTACTCTGCCCGGCAGCGCGCAAC
ACAAGGAGGGTGAGAGCAGC

Downstream 100 bases:

>100_bases
AGCGGAAAACGGTGTCACCTTCTGGCGGCACCGTTTCACGGTTGGGGGCAAAAAGAACGGCCAACTTCAGGCGGATGCCG
ACTCGATCACTCGCACCTCA

Product: purine phosphorylases family protein 1

Products: Adenine; S-Ribosyl-L-homocysteine [C]

Alternate protein names: Nucleoside Phosphorylase; MTA/SAH Nucleosidase; Purine Phosphorylases Family

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MKIVIVTAMPEESAAVMKRAGGLKKTLLESRKRYGFSMCGHELILVESGMGMLNAGWAAAALAAEGADLMISAGFGGAVL
EGLGVGELVMARQVLHWSGSELEEVAVGFYGLNAVADSLSLRLATFVSCDQILNKRDLALRLPPQATNPVVEMESAAVAR
VAASHGIPFLAMRAISDPWDEEMDFTIDEFCDDSMRIRPTKVLATILRRPRIIPQLVRLARTSRVAAGGLARGTERLLAA
I

Sequences:

>Translated_241_residues
MKIVIVTAMPEESAAVMKRAGGLKKTLLESRKRYGFSMCGHELILVESGMGMLNAGWAAAALAAEGADLMISAGFGGAVL
EGLGVGELVMARQVLHWSGSELEEVAVGFYGLNAVADSLSLRLATFVSCDQILNKRDLALRLPPQATNPVVEMESAAVAR
VAASHGIPFLAMRAISDPWDEEMDFTIDEFCDDSMRIRPTKVLATILRRPRIIPQLVRLARTSRVAAGGLARGTERLLAA
I
>Mature_241_residues
MKIVIVTAMPEESAAVMKRAGGLKKTLLESRKRYGFSMCGHELILVESGMGMLNAGWAAAALAAEGADLMISAGFGGAVL
EGLGVGELVMARQVLHWSGSELEEVAVGFYGLNAVADSLSLRLATFVSCDQILNKRDLALRLPPQATNPVVEMESAAVAR
VAASHGIPFLAMRAISDPWDEEMDFTIDEFCDDSMRIRPTKVLATILRRPRIIPQLVRLARTSRVAAGGLARGTERLLAA
I

Specific function: Responsible For Cleavage Of The Glycosidic Bond In Both 5'-Methylthioadenosine (Mta) And S-Adenosylhomocysteine (Sah). [C]

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.2.2.16 [C]

Molecular weight: Translated: 25821; Mature: 25821

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVIVTAMPEESAAVMKRAGGLKKTLLESRKRYGFSMCGHELILVESGMGMLNAGWAAA
CEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHCCHHHCCCEEEEEECCCCCCCCCHHHH
ALAAEGADLMISAGFGGAVLEGLGVGELVMARQVLHWSGSELEEVAVGFYGLNAVADSLS
HHHHCCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LRLATFVSCDQILNKRDLALRLPPQATNPVVEMESAAVARVAASHGIPFLAMRAISDPWD
HHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
EEMDFTIDEFCDDSMRIRPTKVLATILRRPRIIPQLVRLARTSRVAAGGLARGTERLLAA
CCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHC
I
C
>Mature Secondary Structure
MKIVIVTAMPEESAAVMKRAGGLKKTLLESRKRYGFSMCGHELILVESGMGMLNAGWAAA
CEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHCCHHHCCCEEEEEECCCCCCCCCHHHH
ALAAEGADLMISAGFGGAVLEGLGVGELVMARQVLHWSGSELEEVAVGFYGLNAVADSLS
HHHHCCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LRLATFVSCDQILNKRDLALRLPPQATNPVVEMESAAVARVAASHGIPFLAMRAISDPWD
HHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
EEMDFTIDEFCDDSMRIRPTKVLATILRRPRIIPQLVRLARTSRVAAGGLARGTERLLAA
CCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1.8 {5'-methylthioadenosine}} 0.0004 {5'-methylthioadenosine}} 0.00031 {5'-methylthioadenosine}} 3 {S-adenosylhomocysteine}} 0.0043 {S-adenosylhomocysteine}} [C]

Substrates: S-Adenosyl-L-homocysteine; H2O [C]

Specific reaction: S-Adenosyl-L-homocysteine + H2O --> Adenine + S-Ribosyl-L-homocysteine [C]

General reaction: N-Glycosyl bond hydrolysis [C]

Inhibitor: 5'-Chlor of ormycin; 5'-Ethyl thioadenosine; 5'-Methyl thi of ormycin; 5'-Methyl thioinosine; 5'-Methyl thiotubercidin; 5'-n-Propyl thioadenosine; Adenine; AMP; L-Methionine; N-2-Hydroxy ethyl piperazine-N'-2-ethanesulfonic acid; S-8-Aza-Adenosyl homocyst

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA