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The map label for this gene is speE [H]

Identifier: 118480247

GI number: 118480247

Start: 4950472

End: 4951599

Strand: Direct

Name: speE [H]

Synonym: BALH_4705

Alternate gene names: 118480247

Gene position: 4950472-4951599 (Clockwise)

Preceding gene: 118480241

Following gene: 118480248

Centisome position: 94.17

GC content: 35.55

Gene sequence:

>1128_bases
ATGCCAAAACATAGAAAACAAAGCAAAATAAAGATTTATAGAATTGCAAGCTATAAAAAAGACAAGCGTTCTGAATTAGA
CTCTGACAAATTTGAACTTGAACAGCAGGAGGTAGAAAATAAGCAGGGCAAGCACAGTAAACAAGACAAGCAGGACAAAA
AAGATAATCTGGACAAGCAAGACAATCTGGACAAGCAAGTACAATCGGAAAATGTAGTCATAGTACCCACAGATTCACAC
AACTTAGATATATGGGATGAAATTTCTCTAAAGCAAATACAAGCTGGTGAACATACAAATTTATTTGCGGAACAAAGTAA
TTATCAAAATATTAATTTGTTGCAAGTTAGCGATATCCGTTTATATTTGGACAAGCAACTACAATTCAGTTCCGTCGATG
AGCAAATTTATCATGAAGCACTTGTACATCCAATTATGTCAAAAGTAATTGATCCAAAACGTGTTCTCATATTAGGCGGT
GGCGATGGCCTTGCCCTGCGAGAAGTTTTAAAATATGAAACTGTACTACATGTAGACCTTGTTGACTTAGATGGATCGAT
GATTGATATGGCTCGTAATGTTCCTGAATTAGTTTCTTTAAACAAAAATGCATTTTTTGATAATCGTGTAAATACACACG
TATGTGATGCAAAAGAATTTTTAAGCTCTCCTTCTTCTTTATACGATGTAATCATTATTGATTTCCCAGACCCAGCGACA
GAGTTATTAAGTACTTTATATACAAGCGAACTTTTTGCTCGTATAGCTACATTCTTAACAGAAGACGGCGCGTTCGTCTG
TCAATCTAATTCACCTGCTGATGCACCCCTTGTATATTGGAGTATCGGTAACACAATTGAACATGCGGGATTAACTATGA
AGAGTTATCATACAATCGTTCCTTCTTTCGGAACTGACTGGGGATTTCATATCGCTGCTAATTCTGCCGATGTACTCAAT
CAAATTGAACAATTATACGTAGTACCAACTCCTCGAACATTACCTTCCCTTCTTTTTCCTTTATTTCAATTTAAAGAAGA
ACATTTAGAACAACGTAATTTCGCTCTTCTAAACTCAGAATCGAACCTTATCCTACACCAATGTTACAAAAAGGAAATGG
AGTTTTGA

Upstream 100 bases:

>100_bases
ATAAACCTCTCTTGAACACACTCTTCTATCTATTGTCCAAGCAAACTCTACTAAATTACATAGAATAGAATAAAATAACA
CAATTCGAAAGGAGATTCCT

Downstream 100 bases:

>100_bases
CGATATCACAGGAAGGAGTGCTACCTTATGGAATATTCTACTTTCGGTAAGCATATAATAGTAGATTTGTGGGGAGTGGA
TTTTTCCCTATTAGATGATA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 2; PAPT 2; SPDSY 2 [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MPKHRKQSKIKIYRIASYKKDKRSELDSDKFELEQQEVENKQGKHSKQDKQDKKDNLDKQDNLDKQVQSENVVIVPTDSH
NLDIWDEISLKQIQAGEHTNLFAEQSNYQNINLLQVSDIRLYLDKQLQFSSVDEQIYHEALVHPIMSKVIDPKRVLILGG
GDGLALREVLKYETVLHVDLVDLDGSMIDMARNVPELVSLNKNAFFDNRVNTHVCDAKEFLSSPSSLYDVIIIDFPDPAT
ELLSTLYTSELFARIATFLTEDGAFVCQSNSPADAPLVYWSIGNTIEHAGLTMKSYHTIVPSFGTDWGFHIAANSADVLN
QIEQLYVVPTPRTLPSLLFPLFQFKEEHLEQRNFALLNSESNLILHQCYKKEMEF

Sequences:

>Translated_375_residues
MPKHRKQSKIKIYRIASYKKDKRSELDSDKFELEQQEVENKQGKHSKQDKQDKKDNLDKQDNLDKQVQSENVVIVPTDSH
NLDIWDEISLKQIQAGEHTNLFAEQSNYQNINLLQVSDIRLYLDKQLQFSSVDEQIYHEALVHPIMSKVIDPKRVLILGG
GDGLALREVLKYETVLHVDLVDLDGSMIDMARNVPELVSLNKNAFFDNRVNTHVCDAKEFLSSPSSLYDVIIIDFPDPAT
ELLSTLYTSELFARIATFLTEDGAFVCQSNSPADAPLVYWSIGNTIEHAGLTMKSYHTIVPSFGTDWGFHIAANSADVLN
QIEQLYVVPTPRTLPSLLFPLFQFKEEHLEQRNFALLNSESNLILHQCYKKEMEF
>Mature_374_residues
PKHRKQSKIKIYRIASYKKDKRSELDSDKFELEQQEVENKQGKHSKQDKQDKKDNLDKQDNLDKQVQSENVVIVPTDSHN
LDIWDEISLKQIQAGEHTNLFAEQSNYQNINLLQVSDIRLYLDKQLQFSSVDEQIYHEALVHPIMSKVIDPKRVLILGGG
DGLALREVLKYETVLHVDLVDLDGSMIDMARNVPELVSLNKNAFFDNRVNTHVCDAKEFLSSPSSLYDVIIIDFPDPATE
LLSTLYTSELFARIATFLTEDGAFVCQSNSPADAPLVYWSIGNTIEHAGLTMKSYHTIVPSFGTDWGFHIAANSADVLNQ
IEQLYVVPTPRTLPSLLFPLFQFKEEHLEQRNFALLNSESNLILHQCYKKEMEF

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=173, Percent_Identity=29.4797687861272, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786312, Length=172, Percent_Identity=30.8139534883721, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI212645616, Length=174, Percent_Identity=35.632183908046, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25154106, Length=173, Percent_Identity=35.8381502890173, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323175, Length=233, Percent_Identity=30.9012875536481, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6325326, Length=174, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24645443, Length=171, Percent_Identity=27.4853801169591, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 42929; Mature: 42798

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKHRKQSKIKIYRIASYKKDKRSELDSDKFELEQQEVENKQGKHSKQDKQDKKDNLDKQ
CCCCCCCCCEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
DNLDKQVQSENVVIVPTDSHNLDIWDEISLKQIQAGEHTNLFAEQSNYQNINLLQVSDIR
CCHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHCCCCCCCEEECCCCCCCEEEEEEECEE
LYLDKQLQFSSVDEQIYHEALVHPIMSKVIDPKRVLILGGGDGLALREVLKYETVLHVDL
EEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHEEEEEE
VDLDGSMIDMARNVPELVSLNKNAFFDNRVNTHVCDAKEFLSSPSSLYDVIIIDFPDPAT
EECCCHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEECCCCHH
ELLSTLYTSELFARIATFLTEDGAFVCQSNSPADAPLVYWSIGNTIEHAGLTMKSYHTIV
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCCHHHCCCCHHHHHEEC
PSFGTDWGFHIAANSADVLNQIEQLYVVPTPRTLPSLLFPLFQFKEEHLEQRNFALLNSE
CCCCCCCCEEEECCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEECCC
SNLILHQCYKKEMEF
CCHHHHHHHHHHCCC
>Mature Secondary Structure 
PKHRKQSKIKIYRIASYKKDKRSELDSDKFELEQQEVENKQGKHSKQDKQDKKDNLDKQ
CCCCCCCCEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
DNLDKQVQSENVVIVPTDSHNLDIWDEISLKQIQAGEHTNLFAEQSNYQNINLLQVSDIR
CCHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHCCCCCCCEEECCCCCCCEEEEEEECEE
LYLDKQLQFSSVDEQIYHEALVHPIMSKVIDPKRVLILGGGDGLALREVLKYETVLHVDL
EEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHEEEEEE
VDLDGSMIDMARNVPELVSLNKNAFFDNRVNTHVCDAKEFLSSPSSLYDVIIIDFPDPAT
EECCCHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEECCCCHH
ELLSTLYTSELFARIATFLTEDGAFVCQSNSPADAPLVYWSIGNTIEHAGLTMKSYHTIV
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCCHHHCCCCHHHHHEEC
PSFGTDWGFHIAANSADVLNQIEQLYVVPTPRTLPSLLFPLFQFKEEHLEQRNFALLNSE
CCCCCCCCEEEECCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEECCC
SNLILHQCYKKEMEF
CCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]