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The map label for this gene is brnQ [H]

Identifier: 118479699

GI number: 118479699

Start: 4350488

End: 4351930

Strand: Reverse

Name: brnQ [H]

Synonym: BALH_4133

Alternate gene names: 118479699

Gene position: 4351930-4350488 (Counterclockwise)

Preceding gene: 118479700

Following gene: 118479698

Centisome position: 82.78

GC content: 35.34

Gene sequence:

>1443_bases
TTGTTAGGGACGGGTGTTTTTTGCGTTCTTTTACGGGGAAGTATGCTTGAAAGAGGGCATATGACGGGTTTTAATTCAGA
ATATTTTGTTAATAAATCATTTAGGAGGTTAGTAGAAATGAAATCATCTTTAAAGTTTTCTGAGATGTTTGCAATAAGTT
TAATGTTATTTGCACTATTTTTCGGTGCAGGTAATATGATTTTCCCACCAGCGTTAGGACAAGGCGCTGGAACAGATGTA
TGGATCGCATTGTTTGGTTTTATTGTAACAGGCGTTGGGTTGCCTTTATTAGGAGTTATCGTTATAGCTCTTAAAGGAGA
TATTAATGAGTTAGCAGGGCGTGTACACCCTATATTCGCACTAGTTTTTATTACTGCAATTTATTTATGTTTAGGTGTAT
TCGTAAGCGTGCCGCGTACAGGAACAGTTGCTTATGAAATGAGTGTTGCACCGTTCTTACCAAGTGAGATAGCTGGTCAA
TCGTACCCGCTTGTTATCTTTACATTTATTTTCTTTGCTGTAACTTTTTATTTAGCTTTAAACCCATCAAAATTAGTGGG
CCGCATCGGTAAAGTATTAACTCCGATTTTATTAGCTGTTATTGCAATTATTGTAGTGAAAGCACTTATTACACCGATTG
GAGAATTTGGTGCACCGACAGAAGCATATAGTGCTCCGCTCTTTAAAGGTTTTATTGACGGATATTTAACTTTAGATGGA
CTTGCAGCACTTGTTTTCGGAAATGTTGTTATTCATGCTTTAAAAGGAAAAGGTATTACAAATAAAAATAGCATTGCAAA
AGTAACAATTTTTGCAGGATTTATTGCAGCACTTGGTTTACTACTCGTTTACTTAGCGCTTGCTTATCTGGGAGCTTCTA
GTGTCTCTCTTGGAATGGGAGCAAATGGAGGAATTATTTTAACAAACGTTGTAAATCATTTATTCGGTAGTTACGGAACT
TTATTATTAGGAATCGCAATTTCAGCAGCATGTTTAACAACTTCAGTAGGGATTGTCGCAGCTTGCGGAGAATATTTCTC
CTCTTTATTACCGAAGCTTTCTTATCAAAAAGTTGTTTTCATTTTCTGTGTATTAGCATTTATGGTAGCGAATCTTGGTT
TAACTCAGTTAAATGCATTAGCATTACCAATCTTAATCGCAATTTACCCAATTGGTATCGTGCTGATTATATTATCACTT
GTTGAAAACTATATTCGTCTTCCTTTAGTAATGTATGTAGGTGGTATTGTAGGAGCATTCATGATTAGCTTCTTTGATGG
ATTACACAATGCAAATCTTGAGATTGCAGCATTAACACCAATTTTAAATAAAATTCCATTATATAGTGTTGGGATTGGTT
GGTTCGTTCCTGCTCTAGTAGGTATTGTAATTGGATATGTCATTTCATTGTTTCAAAAGCAAGAAGTTGCTGTAGAAAAA
TAG

Upstream 100 bases:

>100_bases
AAACCGTTATCAATTTACACGAGCTATAAAATGAGCTTATTTCGTTATAAATTCATTTTATTAGAATAAGGGTGGAACCA
CGATTTCAACACTCGTCCCT

Downstream 100 bases:

>100_bases
AGAAAAGAGGCTTTCTATGAAAGAAAGCCTCTTTTTTTGTAGAAAAAGCCGTATATAAGGAAATTTAGATATTTAAAGTT
TTATACGTAATATAGTTGAC

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MLGTGVFCVLLRGSMLERGHMTGFNSEYFVNKSFRRLVEMKSSLKFSEMFAISLMLFALFFGAGNMIFPPALGQGAGTDV
WIALFGFIVTGVGLPLLGVIVIALKGDINELAGRVHPIFALVFITAIYLCLGVFVSVPRTGTVAYEMSVAPFLPSEIAGQ
SYPLVIFTFIFFAVTFYLALNPSKLVGRIGKVLTPILLAVIAIIVVKALITPIGEFGAPTEAYSAPLFKGFIDGYLTLDG
LAALVFGNVVIHALKGKGITNKNSIAKVTIFAGFIAALGLLLVYLALAYLGASSVSLGMGANGGIILTNVVNHLFGSYGT
LLLGIAISAACLTTSVGIVAACGEYFSSLLPKLSYQKVVFIFCVLAFMVANLGLTQLNALALPILIAIYPIGIVLIILSL
VENYIRLPLVMYVGGIVGAFMISFFDGLHNANLEIAALTPILNKIPLYSVGIGWFVPALVGIVIGYVISLFQKQEVAVEK

Sequences:

>Translated_480_residues
MLGTGVFCVLLRGSMLERGHMTGFNSEYFVNKSFRRLVEMKSSLKFSEMFAISLMLFALFFGAGNMIFPPALGQGAGTDV
WIALFGFIVTGVGLPLLGVIVIALKGDINELAGRVHPIFALVFITAIYLCLGVFVSVPRTGTVAYEMSVAPFLPSEIAGQ
SYPLVIFTFIFFAVTFYLALNPSKLVGRIGKVLTPILLAVIAIIVVKALITPIGEFGAPTEAYSAPLFKGFIDGYLTLDG
LAALVFGNVVIHALKGKGITNKNSIAKVTIFAGFIAALGLLLVYLALAYLGASSVSLGMGANGGIILTNVVNHLFGSYGT
LLLGIAISAACLTTSVGIVAACGEYFSSLLPKLSYQKVVFIFCVLAFMVANLGLTQLNALALPILIAIYPIGIVLIILSL
VENYIRLPLVMYVGGIVGAFMISFFDGLHNANLEIAALTPILNKIPLYSVGIGWFVPALVGIVIGYVISLFQKQEVAVEK
>Mature_480_residues
MLGTGVFCVLLRGSMLERGHMTGFNSEYFVNKSFRRLVEMKSSLKFSEMFAISLMLFALFFGAGNMIFPPALGQGAGTDV
WIALFGFIVTGVGLPLLGVIVIALKGDINELAGRVHPIFALVFITAIYLCLGVFVSVPRTGTVAYEMSVAPFLPSEIAGQ
SYPLVIFTFIFFAVTFYLALNPSKLVGRIGKVLTPILLAVIAIIVVKALITPIGEFGAPTEAYSAPLFKGFIDGYLTLDG
LAALVFGNVVIHALKGKGITNKNSIAKVTIFAGFIAALGLLLVYLALAYLGASSVSLGMGANGGIILTNVVNHLFGSYGT
LLLGIAISAACLTTSVGIVAACGEYFSSLLPKLSYQKVVFIFCVLAFMVANLGLTQLNALALPILIAIYPIGIVLIILSL
VENYIRLPLVMYVGGIVGAFMISFFDGLHNANLEIAALTPILNKIPLYSVGIGWFVPALVGIVIGYVISLFQKQEVAVEK

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=444, Percent_Identity=36.2612612612613, Blast_Score=275, Evalue=4e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 50968; Mature: 50968

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGTGVFCVLLRGSMLERGHMTGFNSEYFVNKSFRRLVEMKSSLKFSEMFAISLMLFALF
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
FGAGNMIFPPALGQGAGTDVWIALFGFIVTGVGLPLLGVIVIALKGDINELAGRVHPIFA
HCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LVFITAIYLCLGVFVSVPRTGTVAYEMSVAPFLPSEIAGQSYPLVIFTFIFFAVTFYLAL
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHC
NPSKLVGRIGKVLTPILLAVIAIIVVKALITPIGEFGAPTEAYSAPLFKGFIDGYLTLDG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHH
LAALVFGNVVIHALKGKGITNKNSIAKVTIFAGFIAALGLLLVYLALAYLGASSVSLGMG
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
ANGGIILTNVVNHLFGSYGTLLLGIAISAACLTTSVGIVAACGEYFSSLLPKLSYQKVVF
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IFCVLAFMVANLGLTQLNALALPILIAIYPIGIVLIILSLVENYIRLPLVMYVGGIVGAF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MISFFDGLHNANLEIAALTPILNKIPLYSVGIGWFVPALVGIVIGYVISLFQKQEVAVEK
HHHHHHHHCCCCEEEEEHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLGTGVFCVLLRGSMLERGHMTGFNSEYFVNKSFRRLVEMKSSLKFSEMFAISLMLFALF
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
FGAGNMIFPPALGQGAGTDVWIALFGFIVTGVGLPLLGVIVIALKGDINELAGRVHPIFA
HCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LVFITAIYLCLGVFVSVPRTGTVAYEMSVAPFLPSEIAGQSYPLVIFTFIFFAVTFYLAL
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHC
NPSKLVGRIGKVLTPILLAVIAIIVVKALITPIGEFGAPTEAYSAPLFKGFIDGYLTLDG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHH
LAALVFGNVVIHALKGKGITNKNSIAKVTIFAGFIAALGLLLVYLALAYLGASSVSLGMG
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
ANGGIILTNVVNHLFGSYGTLLLGIAISAACLTTSVGIVAACGEYFSSLLPKLSYQKVVF
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IFCVLAFMVANLGLTQLNALALPILIAIYPIGIVLIILSLVENYIRLPLVMYVGGIVGAF
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MISFFDGLHNANLEIAALTPILNKIPLYSVGIGWFVPALVGIVIGYVISLFQKQEVAVEK
HHHHHHHHCCCCEEEEEHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]