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The map label for this gene is pykA [H]

Identifier: 118478626

GI number: 118478626

Start: 3198350

End: 3199450

Strand: Reverse

Name: pykA [H]

Synonym: BALH_3005

Alternate gene names: 118478626

Gene position: 3199450-3198350 (Counterclockwise)

Preceding gene: 118478627

Following gene: 118478619

Centisome position: 60.86

GC content: 35.79

Gene sequence:

>1101_bases
TTGAAGTTTATGGGAATCCTAATGAAAAAGGATGGTGGAAATATGACAATCGATCGAATTTGTACAATTGGGCCAGCAAG
TAATAATAAAGAAACGTTAGCGCAGTTAATAAACAATGGTATGAAAATTGTTCGTCTAAATTTATCACATGGTACGCATG
AAAGTCATAAAGATATTATTCGTTTAGTGAAATCGTTAGATGATTCTATTAAAATTTTAGGCGATGTACAAGGTCCTAAA
ATAAGGTTAGGTGAAATAAAAGGAGAACAAATTACACTTCAGGCGGGAGATTCTTTTATGTTACGTACGCAACCAGTTAC
AGGGAGTAGTACGGAAGCAAGTATTGATTATGAAGGAATCCCGAATGATGTGAAAGTCGGAAGTAGAATTTTAATGAATG
ATGGTGAAGTTGAGTTAATTGTTGAGAAGGTAAGTACGGATAAAATAGAAACAAAGGTCAAAACAGGTGGTAATATCTCA
TCACATAAAGGAGTGAACTTACCAGGGGCAATCGTTAGTTTACCAGCTATTACAGAGAAAGATAAAAAAGATATTCAGTT
TCTTTTAGAAGAGGATGTTGATTTTATTGCGTGTTCTTTCGTAAGAAAACCTAGTCATATAAAAGAAATACGAGATTTTA
TACAACAGTATAAAGAAACATCACCTAATTTAATTGCAAAAATAGAAACGATGGAAGCAATCGAGAATTTTCAAGATATA
TGTAAAGAAGCAGACGGAATTATGATTGCAAGGGGCGATTTAGGAGTAGAGTTGCCGTTCCAATTTATTCCGCTCTTGCA
GAAAATGATGATTCAGGAATGTAATCGAACGAATACATATGTTATTACAGCGACACAAATGCTTCAATCTATGGTAGATC
ATTCGATTCCAACAAGGGCTGAGGTAACTGATGTGTTTCAAGCTGTACTGGATGGAACGAATGCTGTTATGCTTTCTGCT
GAAAGTGCTTCAGGAGAGCATCCGGTTGAAAGTGTGAGTACACTACGTCTCGTTTCTGAATTTGCTGAGCATGTGAAACA
AGACGGTCCTTTTGTGATGAAAGATGTACTGGAATTGCTGCATAAATCTTTGGGTGAGTGA

Upstream 100 bases:

>100_bases
CGACGAAAACGAAAAAAGATTTAAATCAATTATTAACAAAACTTTCGTAGTTTCAAAAGCCTACTTGTGTGAGTAGGCTT
TTTTTACGTTGAGAGTGTAA

Downstream 100 bases:

>100_bases
AAAATAAAAAGAGGGCGGATATGTATCCGTCCTCGTGTGTTTCATTCTATTCTTGCTCTTGCTCATCCAAAATCTTTTCA
AACGCAGCAGATACTTTTTC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKFMGILMKKDGGNMTIDRICTIGPASNNKETLAQLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKILGDVQGPK
IRLGEIKGEQITLQAGDSFMLRTQPVTGSSTEASIDYEGIPNDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNIS
SHKGVNLPGAIVSLPAITEKDKKDIQFLLEEDVDFIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDI
CKEADGIMIARGDLGVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSA
ESASGEHPVESVSTLRLVSEFAEHVKQDGPFVMKDVLELLHKSLGE

Sequences:

>Translated_366_residues
MKFMGILMKKDGGNMTIDRICTIGPASNNKETLAQLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKILGDVQGPK
IRLGEIKGEQITLQAGDSFMLRTQPVTGSSTEASIDYEGIPNDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNIS
SHKGVNLPGAIVSLPAITEKDKKDIQFLLEEDVDFIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDI
CKEADGIMIARGDLGVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSA
ESASGEHPVESVSTLRLVSEFAEHVKQDGPFVMKDVLELLHKSLGE
>Mature_366_residues
MKFMGILMKKDGGNMTIDRICTIGPASNNKETLAQLINNGMKIVRLNLSHGTHESHKDIIRLVKSLDDSIKILGDVQGPK
IRLGEIKGEQITLQAGDSFMLRTQPVTGSSTEASIDYEGIPNDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNIS
SHKGVNLPGAIVSLPAITEKDKKDIQFLLEEDVDFIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDI
CKEADGIMIARGDLGVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRAEVTDVFQAVLDGTNAVMLSA
ESASGEHPVESVSTLRLVSEFAEHVKQDGPFVMKDVLELLHKSLGE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=340, Percent_Identity=43.2352941176471, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI33286420, Length=340, Percent_Identity=43.2352941176471, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI33286418, Length=340, Percent_Identity=43.2352941176471, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI10835121, Length=340, Percent_Identity=40.8823529411765, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI32967597, Length=340, Percent_Identity=40.8823529411765, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI310128732, Length=283, Percent_Identity=41.696113074205, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI310128730, Length=283, Percent_Identity=41.696113074205, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI310128736, Length=231, Percent_Identity=39.3939393939394, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI310128734, Length=231, Percent_Identity=39.3939393939394, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=37.2093023255814, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI1787965, Length=332, Percent_Identity=42.7710843373494, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1788160, Length=335, Percent_Identity=40.5970149253731, Blast_Score=232, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI71984413, Length=336, Percent_Identity=45.8333333333333, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17506829, Length=336, Percent_Identity=45.8333333333333, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71984406, Length=336, Percent_Identity=45.8333333333333, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17506831, Length=336, Percent_Identity=45.8333333333333, Blast_Score=275, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI17544584, Length=350, Percent_Identity=41.7142857142857, Blast_Score=266, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6319279, Length=336, Percent_Identity=38.3928571428571, Blast_Score=220, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6324923, Length=334, Percent_Identity=36.5269461077844, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI28571814, Length=340, Percent_Identity=42.9411764705882, Blast_Score=268, Evalue=6e-72,
Organism=Drosophila melanogaster, GI24648964, Length=340, Percent_Identity=42.9411764705882, Blast_Score=267, Evalue=7e-72,
Organism=Drosophila melanogaster, GI24648966, Length=322, Percent_Identity=39.7515527950311, Blast_Score=248, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24581235, Length=339, Percent_Identity=34.5132743362832, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI24646914, Length=182, Percent_Identity=39.5604395604396, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 40392; Mature: 40392

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFMGILMKKDGGNMTIDRICTIGPASNNKETLAQLINNGMKIVRLNLSHGTHESHKDII
CCCEEEEEECCCCCEEHHHEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHH
RLVKSLDDSIKILGDVQGPKIRLGEIKGEQITLQAGDSFMLRTQPVTGSSTEASIDYEGI
HHHHHCCHHHEEEECCCCCEEEEEECCCCEEEEEECCCEEEEECCCCCCCCCCEEECCCC
PNDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVSLPAITEK
CCCHHHCCEEEECCCCEEEEEEHHCCHHHHHHCCCCCCCCCCCCCCCCHHHEECCCCCCC
DKKDIQFLLEEDVDFIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDI
CHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHH
CKEADGIMIARGDLGVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRA
HHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCHH
EVTDVFQAVLDGTNAVMLSAESASGEHPVESVSTLRLVSEFAEHVKQDGPFVMKDVLELL
HHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
HKSLGE
HHHHCC
>Mature Secondary Structure
MKFMGILMKKDGGNMTIDRICTIGPASNNKETLAQLINNGMKIVRLNLSHGTHESHKDII
CCCEEEEEECCCCCEEHHHEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHH
RLVKSLDDSIKILGDVQGPKIRLGEIKGEQITLQAGDSFMLRTQPVTGSSTEASIDYEGI
HHHHHCCHHHEEEECCCCCEEEEEECCCCEEEEEECCCEEEEECCCCCCCCCCEEECCCC
PNDVKVGSRILMNDGEVELIVEKVSTDKIETKVKTGGNISSHKGVNLPGAIVSLPAITEK
CCCHHHCCEEEECCCCEEEEEEHHCCHHHHHHCCCCCCCCCCCCCCCCHHHEECCCCCCC
DKKDIQFLLEEDVDFIACSFVRKPSHIKEIRDFIQQYKETSPNLIAKIETMEAIENFQDI
CHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHH
CKEADGIMIARGDLGVELPFQFIPLLQKMMIQECNRTNTYVITATQMLQSMVDHSIPTRA
HHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCHH
EVTDVFQAVLDGTNAVMLSAESASGEHPVESVSTLRLVSEFAEHVKQDGPFVMKDVLELL
HHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
HKSLGE
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]