Click here to switch to the map view.

The map label for this gene is 118478597

Identifier: 118478597

GI number: 118478597

Start: 3160580

End: 3162118

Strand: Reverse

Name: 118478597

Synonym: BALH_2974

Alternate gene names: NA

Gene position: 3162118-3160580 (Counterclockwise)

Preceding gene: 118478599

Following gene: 118478596

Centisome position: 60.15

GC content: 33.79

Gene sequence:

>1539_bases
GTGATTTTTCTGAATATTAAGAAAAACAATAAAAGAAGAAAGTTCCTTGCATGTTTATTAGTTAGTCTATGTACCATTCA
TTATTCGTCTATTTCTTTTGCAGAAACACAAGCCGGTAATGCAACTGATGCAATAAAAAATGCTAATGATATTAATACTG
GTATAGCAAATCTAAAGTATGATAGTAGAGATATTTTAGCAGTAAATGGTGATAAAGTAGAGAGTTTTGTTCCGAAAGAG
AGTATCAATTCAAACGGTAAATTTGTAGTAGTGGAACGCGAGAAAAAATCACTTACAACGTCACCAGTCGATATTTCGAT
TATTGATTCTGTAGCAAATCGCACATATCCAGGAGCTGTACAACTTGCCAATAAAGCTTTTGCAGACAATCAACCAAGTT
TATTAGTGGCTAAGAGAAAGCCTTTGAATATTAGTATAGACTTACCTGGCATGAGAAAAGAAAATACAATCACTGTCCAA
AATCCGACATATGGTAATGTGGCTGGAGCAGTAGACGATTTAGTATCTACTTGGAATGAAAAGTATTCTACAACACATAC
GTTACCGGCAAGAATGCAGTATACAGAATCTATGGTTTATAGTAAATCACAAATTGCAAGTGCTCTTAATGTTAACGCTA
AATACCTTGATAACAGTCTAAATATTGACTTTAATGCGGTTGCAAATGGAGAGAAAAAAGTGATGGTAGCCGCATATAAA
CAAATATTTTATACTGTAAGTGCTGAACTACCTAACAATCCATCCGATCTTTTTGATAATAGTGTTACTTTTGATGAGTT
AACTCGTAAAGGAGTAAGTAATTCGGCTCCACCTGTTATGGTTTCAAATGTAGCTTATGGTAGAACGGTTTATGTAAAAT
TAGAAACAACATCTAAGAGTAAAGATGTACAAGCTGCTTTTAAAGCTCTACTTAAGAATAACAGCGTCGAAACGAGTGGA
CAGTACAAAGATATTTTTGAAGAAAGTACCTTTACTGCTGTAGTATTAGGCGGAGATGCGAAAGAGCATAACAAGGTTGT
TACAAAAGATTTCAATGAAATCCGAAATATTATTAAAGATAATGCTGAATTAAGTCTTAAAAATCCAGCATACCCAATTT
CTTATACAAGCACTTTCTTAAAAGATAATGCAACTGCTGCTGTTCATAACAATACAGATTATATCGAGACGACAACTACA
GAATATTCAAGTGCTAAAATGACACTTGATCATTACGGTGCTTACGTTGCTCAATTTGATGTATCTTGGGATGAATTCAC
ATTTGATCAAAATGGGAAAGAAGTACTAACACATAAAACATGGGAAGGTAGTGGCAAAGACAAAACAGCTCATTACTCTA
CAGTAATCCCTCTTCCACCAAATTCAAAAAATATAAAAATTGTAGCAAGAGAATGTACAGGTCTTGCATGGGAGTGGTGG
AGAACCATTATTGATGAACAAAATGTTCCATTAACAAATGAAATAAAGGTTTCAATTGGAGGAACAACATTATACCCAAC
TGCTACTATTAGTCATTAG

Upstream 100 bases:

>100_bases
GTAATTCCTTTAGTTCAAGAACTTTTAAAAGCGTTTATGTAAGGTTATTTGTTAGCTATCATGTATCAATCATTATTATA
TCTTACTAAAAAAGAACGGG

Downstream 100 bases:

>100_bases
GTTTGAGTGAAGCGCCTTGTTTATAGGGCGCTTTTTTACTTAGGTGATAGGTAGATCGGCGTTTCTCCCCATCATTATAT
TTTACTCTAATAGTCTATGA

Product: perfringolysin O, thiol-activated cytolysin

Products: NA

Alternate protein names: Fragment [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MIFLNIKKNNKRRKFLACLLVSLCTIHYSSISFAETQAGNATDAIKNANDINTGIANLKYDSRDILAVNGDKVESFVPKE
SINSNGKFVVVEREKKSLTTSPVDISIIDSVANRTYPGAVQLANKAFADNQPSLLVAKRKPLNISIDLPGMRKENTITVQ
NPTYGNVAGAVDDLVSTWNEKYSTTHTLPARMQYTESMVYSKSQIASALNVNAKYLDNSLNIDFNAVANGEKKVMVAAYK
QIFYTVSAELPNNPSDLFDNSVTFDELTRKGVSNSAPPVMVSNVAYGRTVYVKLETTSKSKDVQAAFKALLKNNSVETSG
QYKDIFEESTFTAVVLGGDAKEHNKVVTKDFNEIRNIIKDNAELSLKNPAYPISYTSTFLKDNATAAVHNNTDYIETTTT
EYSSAKMTLDHYGAYVAQFDVSWDEFTFDQNGKEVLTHKTWEGSGKDKTAHYSTVIPLPPNSKNIKIVARECTGLAWEWW
RTIIDEQNVPLTNEIKVSIGGTTLYPTATISH

Sequences:

>Translated_512_residues
MIFLNIKKNNKRRKFLACLLVSLCTIHYSSISFAETQAGNATDAIKNANDINTGIANLKYDSRDILAVNGDKVESFVPKE
SINSNGKFVVVEREKKSLTTSPVDISIIDSVANRTYPGAVQLANKAFADNQPSLLVAKRKPLNISIDLPGMRKENTITVQ
NPTYGNVAGAVDDLVSTWNEKYSTTHTLPARMQYTESMVYSKSQIASALNVNAKYLDNSLNIDFNAVANGEKKVMVAAYK
QIFYTVSAELPNNPSDLFDNSVTFDELTRKGVSNSAPPVMVSNVAYGRTVYVKLETTSKSKDVQAAFKALLKNNSVETSG
QYKDIFEESTFTAVVLGGDAKEHNKVVTKDFNEIRNIIKDNAELSLKNPAYPISYTSTFLKDNATAAVHNNTDYIETTTT
EYSSAKMTLDHYGAYVAQFDVSWDEFTFDQNGKEVLTHKTWEGSGKDKTAHYSTVIPLPPNSKNIKIVARECTGLAWEWW
RTIIDEQNVPLTNEIKVSIGGTTLYPTATISH
>Mature_512_residues
MIFLNIKKNNKRRKFLACLLVSLCTIHYSSISFAETQAGNATDAIKNANDINTGIANLKYDSRDILAVNGDKVESFVPKE
SINSNGKFVVVEREKKSLTTSPVDISIIDSVANRTYPGAVQLANKAFADNQPSLLVAKRKPLNISIDLPGMRKENTITVQ
NPTYGNVAGAVDDLVSTWNEKYSTTHTLPARMQYTESMVYSKSQIASALNVNAKYLDNSLNIDFNAVANGEKKVMVAAYK
QIFYTVSAELPNNPSDLFDNSVTFDELTRKGVSNSAPPVMVSNVAYGRTVYVKLETTSKSKDVQAAFKALLKNNSVETSG
QYKDIFEESTFTAVVLGGDAKEHNKVVTKDFNEIRNIIKDNAELSLKNPAYPISYTSTFLKDNATAAVHNNTDYIETTTT
EYSSAKMTLDHYGAYVAQFDVSWDEFTFDQNGKEVLTHKTWEGSGKDKTAHYSTVIPLPPNSKNIKIVARECTGLAWEWW
RTIIDEQNVPLTNEIKVSIGGTTLYPTATISH

Specific function: Sulfhydryl-activated toxin with hemolytic activity against host red blood cells. Causes cytolysis by forming pores in cholesterol containing host membranes. binding to target membranes, the protein undergoes a major conformation change, leading to its ins

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Host cell membrane; Multi-pass membrane protein (By similarity). Note=Secreted as soluble protein that then inserts into the host cell membrane and forms pores formed by transmembrane beta-strands (By similarity) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiol-activated cytolysin family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001869 [H]

Pfam domain/function: PF01289 Thiol_cytolysin [H]

EC number: NA

Molecular weight: Translated: 56644; Mature: 56644

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00481 THIOL_CYTOLYSINS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFLNIKKNNKRRKFLACLLVSLCTIHYSSISFAETQAGNATDAIKNANDINTGIANLKY
CEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCHHHHCCCCCCCCCCEEEEE
DSRDILAVNGDKVESFVPKESINSNGKFVVVEREKKSLTTSPVDISIIDSVANRTYPGAV
CCCCEEEECCCHHHHHCCHHHCCCCCCEEEEECCCCCCCCCCCEEEEEHHHHCCCCCCHH
QLANKAFADNQPSLLVAKRKPLNISIDLPGMRKENTITVQNPTYGNVAGAVDDLVSTWNE
HHHHHHCCCCCCCEEEEECCCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
KYSTTHTLPARMQYTESMVYSKSQIASALNVNAKYLDNSLNIDFNAVANGEKKVMVAAYK
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEECCCCCEEEHHEEE
QIFYTVSAELPNNPSDLFDNSVTFDELTRKGVSNSAPPVMVSNVAYGRTVYVKLETTSKS
EEEEEEEECCCCCHHHHCCCCCCHHHHHHCCCCCCCCCEEEECCCCCEEEEEEEECCCCC
KDVQAAFKALLKNNSVETSGQYKDIFEESTFTAVVLGGDAKEHNKVVTKDFNEIRNIIKD
CHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEEECCCCHHCCCCHHHHHHHHHHHHHC
NAELSLKNPAYPISYTSTFLKDNATAAVHNNTDYIETTTTEYSSAKMTLDHYGAYVAQFD
CCEEEECCCCCCCCHHHHHHCCCCEEEEECCCCEEEEECCCCCCEEEEHHHCCCEEEEEE
VSWDEFTFDQNGKEVLTHKTWEGSGKDKTAHYSTVIPLPPNSKNIKIVARECTGLAWEWW
CCHHHEEECCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHH
RTIIDEQNVPLTNEIKVSIGGTTLYPTATISH
HHHHCCCCCCCCCEEEEEECCEEEECEEECCC
>Mature Secondary Structure
MIFLNIKKNNKRRKFLACLLVSLCTIHYSSISFAETQAGNATDAIKNANDINTGIANLKY
CEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCHHHHCCCCCCCCCCEEEEE
DSRDILAVNGDKVESFVPKESINSNGKFVVVEREKKSLTTSPVDISIIDSVANRTYPGAV
CCCCEEEECCCHHHHHCCHHHCCCCCCEEEEECCCCCCCCCCCEEEEEHHHHCCCCCCHH
QLANKAFADNQPSLLVAKRKPLNISIDLPGMRKENTITVQNPTYGNVAGAVDDLVSTWNE
HHHHHHCCCCCCCEEEEECCCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
KYSTTHTLPARMQYTESMVYSKSQIASALNVNAKYLDNSLNIDFNAVANGEKKVMVAAYK
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEECCCCCEEEHHEEE
QIFYTVSAELPNNPSDLFDNSVTFDELTRKGVSNSAPPVMVSNVAYGRTVYVKLETTSKS
EEEEEEEECCCCCHHHHCCCCCCHHHHHHCCCCCCCCCEEEECCCCCEEEEEEEECCCCC
KDVQAAFKALLKNNSVETSGQYKDIFEESTFTAVVLGGDAKEHNKVVTKDFNEIRNIIKD
CHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEEECCCCHHCCCCHHHHHHHHHHHHHC
NAELSLKNPAYPISYTSTFLKDNATAAVHNNTDYIETTTTEYSSAKMTLDHYGAYVAQFD
CCEEEECCCCCCCCHHHHHHCCCCEEEEECCCCEEEEECCCCCCEEEEHHHCCCEEEEEE
VSWDEFTFDQNGKEVLTHKTWEGSGKDKTAHYSTVIPLPPNSKNIKIVARECTGLAWEWW
CCHHHEEECCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHH
RTIIDEQNVPLTNEIKVSIGGTTLYPTATISH
HHHHCCCCCCCCCEEEEEECCEEEECEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA