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The map label for this gene is yfkF [H]

Identifier: 118478586

GI number: 118478586

Start: 3149236

End: 3150522

Strand: Reverse

Name: yfkF [H]

Synonym: BALH_2963

Alternate gene names: 118478586

Gene position: 3150522-3149236 (Counterclockwise)

Preceding gene: 118478587

Following gene: 118478585

Centisome position: 59.93

GC content: 38.31

Gene sequence:

>1287_bases
ATGAAAATTATTTCATCCACTAGTTCTTTCTTATTACATAGTAAACAATATATAATGAGTGTGGTGGATTACAAAAGGGA
GAGATTAGGGATGTCGATACGTTTTACTTTTTGGATTATGGTTGGGATTGTAGCAATCTCGGGTTTGTCACAAGGGATGC
TTTTACCGGCCATTGCAATGATTTTTGAACAAGAAGGGGTAAGTTCAAGTATTAACGGTATTCATGCGACGGCGCTATAC
ATTGGGATATTAGTGATCTCACCGTTTCTTGAAAAACCGATGCAAAAGTTTGGAATGAAGCCAATTATTGTGATTGGTGG
GTTTCTCGTTATCATTTCATTATTCTTTTTTACACAAACTTTTTCATTCTGGGTATGGTTTATCCTTAGATTTCTAGTTG
GAGTCGGAGATCATATGCTGCATGTCGGAACACAAACATGGATTACGACAACAGCAGACCCGAGTAAAATAGGGAGACAA
GTGTCGATATACGGTGTATTCTTCGGAATTGGTTTTGCCGTCGGTCCGTATTTAGCAAGCACTGTTCAGTACGGTCTTGC
AACGCCATTTATTATATCTACTATACTTTGTTTAATAGGTTGGCTGTTACTATTACCAACAAAAAATGCATTCCCAGCGC
AAGATGAAAGAGAAGTGAAGCGTGAATCATCATTTTCTCGTTATAAACAAGTTGTTGGATTAGGATGGATTGCATTACTG
GGACCACTTGCATATGGCGTACTGGAAGCGATGTTAAATAGTAACTTACCAGTATACGCGCTTCGAAAAGGATGGTCTGT
CTCAGAAGTATCCTTCTTATTGCCAGCGTTTGCAGTTGGGGGCATTATTACACAAATTCCGCTCGGTATATTAAGTGACA
AATACGGAAGGGACCGTATTTTAACGTGGACGTTCTGCATAAGTACGGGCATTTTCTTACTGGCAGCCGTATTTGATCAA
TATTACTGGATTGTCTTTGCCTGCATGTTGTTAGCGGGTATGGTCATTGGATCCTGCTTCTCGTTAGGACTTGGATTTAT
GACAGATTTATTACCGAGACACTTATTGCCAGCAGGAAATATATTATCTGGAATCGCCTTTAGTTTAGGAAGTATACTCG
GACCTGTATTAGGAGGCGTATTTATAGAAAAAATACAGTATACAAGCTTTTTTGTTGCGGTTATGATTATAATAGGAACT
CTCGCAATATTATATATGATCTACATGAAGAATCAATTTGCATCAAGAAAAATAGAAAGTAGGTTAGGGCATGACAAAAC
AACCTAA

Upstream 100 bases:

>100_bases
GCTTAGATGTATTTATTAAGTATGTAGAGGATTGGTTAGAAGGTAAGTAGGTTGGGGAAGAAGTTAGATGACATGTGTCT
AGCTTCTTTTTTGTGGAAAA

Downstream 100 bases:

>100_bases
TAAAAAGAAATTTGAAGTACTCGAAAACGAAACAATTACAGACTGCTTAGCACGTATGGAACAAGAAGGCTATGCACCGT
CTCGCCGTATGGAAGAGCCA

Product: multidrug-efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKIISSTSSFLLHSKQYIMSVVDYKRERLGMSIRFTFWIMVGIVAISGLSQGMLLPAIAMIFEQEGVSSSINGIHATALY
IGILVISPFLEKPMQKFGMKPIIVIGGFLVIISLFFFTQTFSFWVWFILRFLVGVGDHMLHVGTQTWITTTADPSKIGRQ
VSIYGVFFGIGFAVGPYLASTVQYGLATPFIISTILCLIGWLLLLPTKNAFPAQDEREVKRESSFSRYKQVVGLGWIALL
GPLAYGVLEAMLNSNLPVYALRKGWSVSEVSFLLPAFAVGGIITQIPLGILSDKYGRDRILTWTFCISTGIFLLAAVFDQ
YYWIVFACMLLAGMVIGSCFSLGLGFMTDLLPRHLLPAGNILSGIAFSLGSILGPVLGGVFIEKIQYTSFFVAVMIIIGT
LAILYMIYMKNQFASRKIESRLGHDKTT

Sequences:

>Translated_428_residues
MKIISSTSSFLLHSKQYIMSVVDYKRERLGMSIRFTFWIMVGIVAISGLSQGMLLPAIAMIFEQEGVSSSINGIHATALY
IGILVISPFLEKPMQKFGMKPIIVIGGFLVIISLFFFTQTFSFWVWFILRFLVGVGDHMLHVGTQTWITTTADPSKIGRQ
VSIYGVFFGIGFAVGPYLASTVQYGLATPFIISTILCLIGWLLLLPTKNAFPAQDEREVKRESSFSRYKQVVGLGWIALL
GPLAYGVLEAMLNSNLPVYALRKGWSVSEVSFLLPAFAVGGIITQIPLGILSDKYGRDRILTWTFCISTGIFLLAAVFDQ
YYWIVFACMLLAGMVIGSCFSLGLGFMTDLLPRHLLPAGNILSGIAFSLGSILGPVLGGVFIEKIQYTSFFVAVMIIIGT
LAILYMIYMKNQFASRKIESRLGHDKTT
>Mature_428_residues
MKIISSTSSFLLHSKQYIMSVVDYKRERLGMSIRFTFWIMVGIVAISGLSQGMLLPAIAMIFEQEGVSSSINGIHATALY
IGILVISPFLEKPMQKFGMKPIIVIGGFLVIISLFFFTQTFSFWVWFILRFLVGVGDHMLHVGTQTWITTTADPSKIGRQ
VSIYGVFFGIGFAVGPYLASTVQYGLATPFIISTILCLIGWLLLLPTKNAFPAQDEREVKRESSFSRYKQVVGLGWIALL
GPLAYGVLEAMLNSNLPVYALRKGWSVSEVSFLLPAFAVGGIITQIPLGILSDKYGRDRILTWTFCISTGIFLLAAVFDQ
YYWIVFACMLLAGMVIGSCFSLGLGFMTDLLPRHLLPAGNILSGIAFSLGSILGPVLGGVFIEKIQYTSFFVAVMIIIGT
LAILYMIYMKNQFASRKIESRLGHDKTT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787126, Length=353, Percent_Identity=22.3796033994334, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47266; Mature: 47266

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIISSTSSFLLHSKQYIMSVVDYKRERLGMSIRFTFWIMVGIVAISGLSQGMLLPAIAM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
IFEQEGVSSSINGIHATALYIGILVISPFLEKPMQKFGMKPIIVIGGFLVIISLFFFTQT
HHHHCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FSFWVWFILRFLVGVGDHMLHVGTQTWITTTADPSKIGRQVSIYGVFFGIGFAVGPYLAS
HHHHHHHHHHHHHCCCHHHHHCCCHHEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHH
TVQYGLATPFIISTILCLIGWLLLLPTKNAFPAQDEREVKRESSFSRYKQVVGLGWIALL
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GPLAYGVLEAMLNSNLPVYALRKGWSVSEVSFLLPAFAVGGIITQIPLGILSDKYGRDRI
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LTWTFCISTGIFLLAAVFDQYYWIVFACMLLAGMVIGSCFSLGLGFMTDLLPRHLLPAGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ILSGIAFSLGSILGPVLGGVFIEKIQYTSFFVAVMIIIGTLAILYMIYMKNQFASRKIES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLGHDKTT
HCCCCCCC
>Mature Secondary Structure
MKIISSTSSFLLHSKQYIMSVVDYKRERLGMSIRFTFWIMVGIVAISGLSQGMLLPAIAM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
IFEQEGVSSSINGIHATALYIGILVISPFLEKPMQKFGMKPIIVIGGFLVIISLFFFTQT
HHHHCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FSFWVWFILRFLVGVGDHMLHVGTQTWITTTADPSKIGRQVSIYGVFFGIGFAVGPYLAS
HHHHHHHHHHHHHCCCHHHHHCCCHHEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHH
TVQYGLATPFIISTILCLIGWLLLLPTKNAFPAQDEREVKRESSFSRYKQVVGLGWIALL
HHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GPLAYGVLEAMLNSNLPVYALRKGWSVSEVSFLLPAFAVGGIITQIPLGILSDKYGRDRI
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LTWTFCISTGIFLLAAVFDQYYWIVFACMLLAGMVIGSCFSLGLGFMTDLLPRHLLPAGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ILSGIAFSLGSILGPVLGGVFIEKIQYTSFFVAVMIIIGTLAILYMIYMKNQFASRKIES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLGHDKTT
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]