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The map label for this gene is ooxB [H]

Identifier: 118478187

GI number: 118478187

Start: 2705799

End: 2706974

Strand: Reverse

Name: ooxB [H]

Synonym: BALH_2544

Alternate gene names: 118478187

Gene position: 2706974-2705799 (Counterclockwise)

Preceding gene: 118478192

Following gene: 118478186

Centisome position: 51.49

GC content: 39.88

Gene sequence:

>1176_bases
GTGAGGCACTGCGACGTTTTAATAATTGGCGGTGGTATTATTGGCTGCTCGATCGCTTATTACACTTCAAAATACGGAAG
AGACGTAACAATCATTGAAAAAGGAGAATTTGTCAGCGGGACGTCTTCACGATGTGATGGAAATATTTTGGCTATTGATA
AAGACCCGGGGTTTGATAGTCAAATGTCGTTAGTAAGTCAAAAATTAGTAACGGATTTAAGTGAAGAGTTAGAGCGCTCA
TTTGAATATAGGGCACCAGGAAGTATTCTCGTATGTGAGTCAGACGAAGAAATGGAAGCAGCGCAGCAATGGGTGAATCG
TCAAAAAGAAGCGGGTTTACCGTTTCGAATGCTTGATAGGCAAGATATAAGAGCGGAATCGCCATTCTTTGCAGATGATT
TATTAGGCGGGTTAGAATGCGCAACAGATTCGACTGTAAATCCATATCTTCTTGCTTTTTCACTTCTTGCAGAATCGAAG
AAATATGGTACGAAGGCTTTTAATCATACGGAAGTAAAAGAAATGAAAAGAGATAAAGACGGTTCCTTTATTGTAGAAAC
GACAAATGGGACGTTTACTGCGAAGCAAGTGGTGAACGCAGCGGGTGTGTGGGCTCCGAAAATCGGACAAATGTTGGATG
TAAATATCCCCATTGAACCGAGAAAAGGGCATATTATTGTAGCTTCAAGGCAACAACACGTTGGTTGTCGTAAAGTTATG
GAATTTGGTTATTTAATTTCTAAATTTGGCGGAAAACGAAAAGTGGATGCTTTAACTGAAAAATATGGAGTAGCTCTCGT
ATTTGAGCCGACAGAAAGCCAAAATTTTTTAATTGGTAGTAGTAGAGAGTTTGTGGGGTTTCATACGAAGATAAACAACG
AGGTTATTAAATGTATTGCGAATAGAGCAATTCGTTTTTATCCAAAAATGGCGGATATGATGGTGATTCGTTCATATGCT
GGATTACGCCCGTGGACAGAAGATCATTTGCCGATTATTTCGCGTGTGGAACATATCCCGAATTACTTTATTGCAGCAGG
GCATGAAGGGGATGGCATTAGTCTTGCCGCGGTTACAGGGAAAGTGATTGAAGAGTTATTAAATGAAAAAGAAACAATCA
TTCCTATTGAACCACTTCGTTTGAGTCGTTTTACAGAAAGGGTGTTAAACGGATGA

Upstream 100 bases:

>100_bases
TACAAATAAAAATATTGTTTGTAAAAATTGTATATATGTACAGTTGGCACGTCAATTGCATAAAAGAAAGTGAGAGGTTT
AGGGGAAGGAGGAGTATTTT

Downstream 100 bases:

>100_bases
GGACACAAAAAGTCTTTACGACGATTGATACACATACGGGTGGGAATCCAACGAGAACATTGATTAGCGGACTACCTAAG
TTACTTGGAGAGACGATGGC

Product: sarcosine oxidase, subunit beta

Products: glycine; formaldehyde; H2O2

Alternate protein names: Octopine oxidase subunit B [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MRHCDVLIIGGGIIGCSIAYYTSKYGRDVTIIEKGEFVSGTSSRCDGNILAIDKDPGFDSQMSLVSQKLVTDLSEELERS
FEYRAPGSILVCESDEEMEAAQQWVNRQKEAGLPFRMLDRQDIRAESPFFADDLLGGLECATDSTVNPYLLAFSLLAESK
KYGTKAFNHTEVKEMKRDKDGSFIVETTNGTFTAKQVVNAAGVWAPKIGQMLDVNIPIEPRKGHIIVASRQQHVGCRKVM
EFGYLISKFGGKRKVDALTEKYGVALVFEPTESQNFLIGSSREFVGFHTKINNEVIKCIANRAIRFYPKMADMMVIRSYA
GLRPWTEDHLPIISRVEHIPNYFIAAGHEGDGISLAAVTGKVIEELLNEKETIIPIEPLRLSRFTERVLNG

Sequences:

>Translated_391_residues
MRHCDVLIIGGGIIGCSIAYYTSKYGRDVTIIEKGEFVSGTSSRCDGNILAIDKDPGFDSQMSLVSQKLVTDLSEELERS
FEYRAPGSILVCESDEEMEAAQQWVNRQKEAGLPFRMLDRQDIRAESPFFADDLLGGLECATDSTVNPYLLAFSLLAESK
KYGTKAFNHTEVKEMKRDKDGSFIVETTNGTFTAKQVVNAAGVWAPKIGQMLDVNIPIEPRKGHIIVASRQQHVGCRKVM
EFGYLISKFGGKRKVDALTEKYGVALVFEPTESQNFLIGSSREFVGFHTKINNEVIKCIANRAIRFYPKMADMMVIRSYA
GLRPWTEDHLPIISRVEHIPNYFIAAGHEGDGISLAAVTGKVIEELLNEKETIIPIEPLRLSRFTERVLNG
>Mature_391_residues
MRHCDVLIIGGGIIGCSIAYYTSKYGRDVTIIEKGEFVSGTSSRCDGNILAIDKDPGFDSQMSLVSQKLVTDLSEELERS
FEYRAPGSILVCESDEEMEAAQQWVNRQKEAGLPFRMLDRQDIRAESPFFADDLLGGLECATDSTVNPYLLAFSLLAESK
KYGTKAFNHTEVKEMKRDKDGSFIVETTNGTFTAKQVVNAAGVWAPKIGQMLDVNIPIEPRKGHIIVASRQQHVGCRKVM
EFGYLISKFGGKRKVDALTEKYGVALVFEPTESQNFLIGSSREFVGFHTKINNEVIKCIANRAIRFYPKMADMMVIRSYA
GLRPWTEDHLPIISRVEHIPNYFIAAGHEGDGISLAAVTGKVIEELLNEKETIIPIEPLRLSRFTERVLNG

Specific function: Oxidative cleavage of octopine into L-arginine and pyruvate [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI24797151, Length=398, Percent_Identity=25.8793969849246, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI197927446, Length=378, Percent_Identity=21.957671957672, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI21361378, Length=378, Percent_Identity=21.957671957672, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI194306651, Length=209, Percent_Identity=26.3157894736842, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1787438, Length=436, Percent_Identity=21.5596330275229, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787559, Length=380, Percent_Identity=20.7894736842105, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788574, Length=230, Percent_Identity=23.9130434782609, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71994045, Length=228, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71994052, Length=235, Percent_Identity=25.9574468085106, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20130091, Length=390, Percent_Identity=20, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.5.3.1

Molecular weight: Translated: 43617; Mature: 43617

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHCDVLIIGGGIIGCSIAYYTSKYGRDVTIIEKGEFVSGTSSRCDGNILAIDKDPGFDS
CCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCH
QMSLVSQKLVTDLSEELERSFEYRAPGSILVCESDEEMEAAQQWVNRQKEAGLPFRMLDR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEECCC
QDIRAESPFFADDLLGGLECATDSTVNPYLLAFSLLAESKKYGTKAFNHTEVKEMKRDKD
HHCCCCCCCCHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
GSFIVETTNGTFTAKQVVNAAGVWAPKIGQMLDVNIPIEPRKGHIIVASRQQHVGCRKVM
CCEEEECCCCCEEHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHH
EFGYLISKFGGKRKVDALTEKYGVALVFEPTESQNFLIGSSREFVGFHTKINNEVIKCIA
HHHHHHHHCCCCCHHHHHHHHHCEEEEEECCCCCCEEEECCCCEEEEEEHHHHHHHHHHH
NRAIRFYPKMADMMVIRSYAGLRPWTEDHLPIISRVEHIPNYFIAAGHEGDGISLAAVTG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCEEEEHHHH
KVIEELLNEKETIIPIEPLRLSRFTERVLNG
HHHHHHHCCCCEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRHCDVLIIGGGIIGCSIAYYTSKYGRDVTIIEKGEFVSGTSSRCDGNILAIDKDPGFDS
CCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCH
QMSLVSQKLVTDLSEELERSFEYRAPGSILVCESDEEMEAAQQWVNRQKEAGLPFRMLDR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEECCC
QDIRAESPFFADDLLGGLECATDSTVNPYLLAFSLLAESKKYGTKAFNHTEVKEMKRDKD
HHCCCCCCCCHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
GSFIVETTNGTFTAKQVVNAAGVWAPKIGQMLDVNIPIEPRKGHIIVASRQQHVGCRKVM
CCEEEECCCCCEEHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHH
EFGYLISKFGGKRKVDALTEKYGVALVFEPTESQNFLIGSSREFVGFHTKINNEVIKCIA
HHHHHHHHCCCCCHHHHHHHHHCEEEEEECCCCCCEEEECCCCEEEEEEHHHHHHHHHHH
NRAIRFYPKMADMMVIRSYAGLRPWTEDHLPIISRVEHIPNYFIAAGHEGDGISLAAVTG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCEEEEHHHH
KVIEELLNEKETIIPIEPLRLSRFTERVLNG
HHHHHHHCCCCEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sarcosine; H2O; O2

Specific reaction: sarcosine + H2O + O2 = glycine + formaldehyde + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]