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The map label for this gene is sleB [H]

Identifier: 118478116

GI number: 118478116

Start: 2637814

End: 2638575

Strand: Reverse

Name: sleB [H]

Synonym: BALH_2473

Alternate gene names: 118478116

Gene position: 2638575-2637814 (Counterclockwise)

Preceding gene: 118478118

Following gene: 118478115

Centisome position: 50.19

GC content: 38.58

Gene sequence:

>762_bases
ATGCGCCAAAAAGCTATTTTTAAAATAGCAGTTTTACTTGCGTTCATAGGACTATCTTTAATGGTCAGTAGTATACAACT
AAAAAATGTAGAAGCCTTTTCTAATCAAGTCATTCAAAGGGGAGCATCTGGCGAAGATGTCATTGAACTGCAATCTCGTT
TGAAATATAACGGATTTTATACGGGAAAAGTGGATGGTGTTTTCGGCTGGGGTACATACTGGGCACTTCGAAATTTTCAA
GAGAAATTTGGTTTACCTGTTGATGGTTTAGCTGGAGCTAAAACGAAGCAAATGCTCGTTAAGGCAACGAAGTATGATAA
GTCAACTGCTAATAAAGGAAGTAGTGGTGGTACAGCACAAGAAAATAAACCATCTCAAAATAAAGGGACAAATGTTCCGA
ATGGTTATTCTCAAAATGACATTCAGCTCATGGCAAACGCAGTATATGGAGAGTCTCGTGGTGAGCCGTACTTAGGACAA
GTTGCAGTCGCTGCGGTTATTTTAAATCGCGTTACAAGTGCATCATTTCCAAATACCGTTTCGGGTGTAATCTTTGAGCC
AAGAGCATTTACAGCGGTAGCAGACGGACAAATATATTTAACGCCAAATGAAACAGCGAAAAAGGCTGTATTAGATGCAA
TTAATGGATGGGATCCAACAGGAAACGCATTGTACTACTTCAATCCAGATACTGCAACAAGTAAATGGATTTGGACTCGT
CCACAAATTAAAAAAATCGGGAAACATATTTTCTGTAAATAG

Upstream 100 bases:

>100_bases
CAACGATATGAGCTTGGATTTTTTTGTCTTCTTTACATAAAAGCGAGCCTTTTACAAAACATAACCAAGAAATCATTTCT
GTAATTAAGGAGGGAAATTT

Downstream 100 bases:

>100_bases
AGCGGAGGTGGAACTATGTTACGAGGTATTATAATCGTATTATTAACAATCGGTGTAGTCGGCACGGGGTACTGGGGCTA
TAAAGAGCATCAAGAGAAGA

Product: spore cortex-lytic enzyme

Products: NA

Alternate protein names: SCLE; Germination-specific amidase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MRQKAIFKIAVLLAFIGLSLMVSSIQLKNVEAFSNQVIQRGASGEDVIELQSRLKYNGFYTGKVDGVFGWGTYWALRNFQ
EKFGLPVDGLAGAKTKQMLVKATKYDKSTANKGSSGGTAQENKPSQNKGTNVPNGYSQNDIQLMANAVYGESRGEPYLGQ
VAVAAVILNRVTSASFPNTVSGVIFEPRAFTAVADGQIYLTPNETAKKAVLDAINGWDPTGNALYYFNPDTATSKWIWTR
PQIKKIGKHIFCK

Sequences:

>Translated_253_residues
MRQKAIFKIAVLLAFIGLSLMVSSIQLKNVEAFSNQVIQRGASGEDVIELQSRLKYNGFYTGKVDGVFGWGTYWALRNFQ
EKFGLPVDGLAGAKTKQMLVKATKYDKSTANKGSSGGTAQENKPSQNKGTNVPNGYSQNDIQLMANAVYGESRGEPYLGQ
VAVAAVILNRVTSASFPNTVSGVIFEPRAFTAVADGQIYLTPNETAKKAVLDAINGWDPTGNALYYFNPDTATSKWIWTR
PQIKKIGKHIFCK
>Mature_253_residues
MRQKAIFKIAVLLAFIGLSLMVSSIQLKNVEAFSNQVIQRGASGEDVIELQSRLKYNGFYTGKVDGVFGWGTYWALRNFQ
EKFGLPVDGLAGAKTKQMLVKATKYDKSTANKGSSGGTAQENKPSQNKGTNVPNGYSQNDIQLMANAVYGESRGEPYLGQ
VAVAAVILNRVTSASFPNTVSGVIFEPRAFTAVADGQIYLTPNETAKKAVLDAINGWDPTGNALYYFNPDTATSKWIWTR
PQIKKIGKHIFCK

Specific function: Probable N-acetylmuramyl-L-alanine amidase. Required for spore cortex hydrolysis during germination. May form a complex with some hydrophobic spore component, leading to a stabilization of the enzyme in a spore-bound form [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Forespore. Note=Expressed in the forespore and then transported across the inner forespore membrane and deposited on the outside of the cortex [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224 [H]

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 27618; Mature: 27618

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQKAIFKIAVLLAFIGLSLMVSSIQLKNVEAFSNQVIQRGASGEDVIELQSRLKYNGFY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEE
TGKVDGVFGWGTYWALRNFQEKFGLPVDGLAGAKTKQMLVKATKYDKSTANKGSSGGTAQ
ECCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
ENKPSQNKGTNVPNGYSQNDIQLMANAVYGESRGEPYLGQVAVAAVILNRVTSASFPNTV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
SGVIFEPRAFTAVADGQIYLTPNETAKKAVLDAINGWDPTGNALYYFNPDTATSKWIWTR
CCEEECCCEEEEEECCEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEECC
PQIKKIGKHIFCK
HHHHHHHHHHCCC
>Mature Secondary Structure
MRQKAIFKIAVLLAFIGLSLMVSSIQLKNVEAFSNQVIQRGASGEDVIELQSRLKYNGFY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEE
TGKVDGVFGWGTYWALRNFQEKFGLPVDGLAGAKTKQMLVKATKYDKSTANKGSSGGTAQ
ECCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
ENKPSQNKGTNVPNGYSQNDIQLMANAVYGESRGEPYLGQVAVAAVILNRVTSASFPNTV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
SGVIFEPRAFTAVADGQIYLTPNETAKKAVLDAINGWDPTGNALYYFNPDTATSKWIWTR
CCEEECCCEEEEEECCEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEECC
PQIKKIGKHIFCK
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8752358; 8081503; 10197998 [H]