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The map label for this gene is dnaN [H]

Identifier: 118478062

GI number: 118478062

Start: 2587436

End: 2588566

Strand: Direct

Name: dnaN [H]

Synonym: BALH_2417

Alternate gene names: 118478062

Gene position: 2587436-2588566 (Clockwise)

Preceding gene: 118478061

Following gene: 118478063

Centisome position: 49.22

GC content: 31.83

Gene sequence:

>1131_bases
ATGGAGTTTATCGTTAATCACAAACATTTTACACAAGCACTTTCAGATGTAAGTAAAGCCATATCAACGAAAGCTATAAT
TCCTATATTATCTGGCATAAAAATAACAGCAGATCAATCTGGGATTACTTTAATCGCAAGTAATTCGAATATTTTCATCG
AAAAATTTATACCTAGTGCTATAGATGACGAACAAATTACAACTATCTTACAAGCAGGAACAATTGTTGTACCTGCAAAA
TACTTCATTGAAATTATAAAGAAAATGCCAAGCGATATAGTAATAAAAAGTAAGAATGAGCAAACAATTACCATACAATC
AGGAGAAATCACATTAAACTTAAATGGCTTTCCAGCGAATGAATTTCCTAACGTACCACAAATAGACGATCATACAGAAA
TACAAATAGAAACAAAACAATTGATTGATGCGTTTAAACAAACAGTCTTTGCAGTAGCGAAAAATGAATCTAGACCCGTT
CTTACTGGCGTACATATCGAGTTAGACCATAACAAATTAATATGCGCTGCAACTGACTCTCATAGATTAGCTATACGTGA
AACACTAATTTCTACTAATATGAAAGCGAATTGTATTGTACCAAGTGCAACCATTAATGAGCTTTTAAAATTAATAAACA
GTAATTTAGAATTCGTTTCTATTTATCTATCAGAAAGTCACATTATTTTTACATTCGGCACAACTACGTTATATTCACGA
CTAATTGAAGGTAAATATCCTAATATTTCTACTCTCATTCCAAATGAATTTCAAACCGTCATTAACATAGATAGACAAAG
AATGTTACAAGGGGTAGATCGATCAAGCTTATTAGCGAGTGAATGGGCAAATAACAATGTTAACTTAGAAATTGTAAACG
AATCTACAATTCAAATTTCTTCTAACGCTTCTCAGATCGGAAAAATATCTGAGACGCAACAGATAGATGTAATTCAAGGT
AAAAAACAATTAAATATATCTTTCGATGGACGATTTATGTTGGATGCTTTAAGGGCAATAAAAGAAGAAACGGTTACTTT
AAGTTTCAGCGGTTCTATGAGACCAATATTAATTGAAGCAGGGACACAATCTGCAGCAATCCATCTTATATCTCCAGTAA
GAGCTTATTAA

Upstream 100 bases:

>100_bases
AATATGTTAAAAAAGAAAGAAACGGTACCTTACTTTTTTAACGGAACTATCGTTCATCAATCTTCATAAAAATATTATTT
CATTTTAGGCGGGGGAAAAG

Downstream 100 bases:

>100_bases
AAACGGAGGAAGGAATCAAATTATGTACAAGCACACTTTATGTTTCATAAAAAGAAATGAAGAGATACTTATGCTAAATA
GAAAATATGACCCTGTAAAA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MEFIVNHKHFTQALSDVSKAISTKAIIPILSGIKITADQSGITLIASNSNIFIEKFIPSAIDDEQITTILQAGTIVVPAK
YFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPANEFPNVPQIDDHTEIQIETKQLIDAFKQTVFAVAKNESRPV
LTGVHIELDHNKLICAATDSHRLAIRETLISTNMKANCIVPSATINELLKLINSNLEFVSIYLSESHIIFTFGTTTLYSR
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASEWANNNVNLEIVNESTIQISSNASQIGKISETQQIDVIQG
KKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEAGTQSAAIHLISPVRAY

Sequences:

>Translated_376_residues
MEFIVNHKHFTQALSDVSKAISTKAIIPILSGIKITADQSGITLIASNSNIFIEKFIPSAIDDEQITTILQAGTIVVPAK
YFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPANEFPNVPQIDDHTEIQIETKQLIDAFKQTVFAVAKNESRPV
LTGVHIELDHNKLICAATDSHRLAIRETLISTNMKANCIVPSATINELLKLINSNLEFVSIYLSESHIIFTFGTTTLYSR
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASEWANNNVNLEIVNESTIQISSNASQIGKISETQQIDVIQG
KKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEAGTQSAAIHLISPVRAY
>Mature_376_residues
MEFIVNHKHFTQALSDVSKAISTKAIIPILSGIKITADQSGITLIASNSNIFIEKFIPSAIDDEQITTILQAGTIVVPAK
YFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPANEFPNVPQIDDHTEIQIETKQLIDAFKQTVFAVAKNESRPV
LTGVHIELDHNKLICAATDSHRLAIRETLISTNMKANCIVPSATINELLKLINSNLEFVSIYLSESHIIFTFGTTTLYSR
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASEWANNNVNLEIVNESTIQISSNASQIGKISETQQIDVIQG
KKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEAGTQSAAIHLISPVRAY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=380, Percent_Identity=24.2105263157895, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41620; Mature: 41620

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFIVNHKHFTQALSDVSKAISTKAIIPILSGIKITADQSGITLIASNSNIFIEKFIPSA
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCEEEEECCCCC
IDDEQITTILQAGTIVVPAKYFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPAN
CCHHHHHHHHHCCCEEECHHHHHHHHHHCCCCEEEECCCCCEEEEECCEEEEEECCCCCC
EFPNVPQIDDHTEIQIETKQLIDAFKQTVFAVAKNESRPVLTGVHIELDHNKLICAATDS
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHEECCCCCCEEEEEEEEECCCEEEEEECCC
HRLAIRETLISTNMKANCIVPSATINELLKLINSNLEFVSIYLSESHIIFTFGTTTLYSR
CCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEEECCEEEEEECHHHHHHH
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASEWANNNVNLEIVNESTIQIS
HHCCCCCCCCEECCCHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCEEEEE
SNASQIGKISETQQIDVIQGKKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEA
CCHHHHCCCCCCCEEEEECCCEEEEEEECCHHHHHHHHHHHHCEEEEEECCCCCEEEEEC
GTQSAAIHLISPVRAY
CCCCEEEEEECCCCCC
>Mature Secondary Structure
MEFIVNHKHFTQALSDVSKAISTKAIIPILSGIKITADQSGITLIASNSNIFIEKFIPSA
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCEEEEECCCCC
IDDEQITTILQAGTIVVPAKYFIEIIKKMPSDIVIKSKNEQTITIQSGEITLNLNGFPAN
CCHHHHHHHHHCCCEEECHHHHHHHHHHCCCCEEEECCCCCEEEEECCEEEEEECCCCCC
EFPNVPQIDDHTEIQIETKQLIDAFKQTVFAVAKNESRPVLTGVHIELDHNKLICAATDS
CCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHEECCCCCCEEEEEEEEECCCEEEEEECCC
HRLAIRETLISTNMKANCIVPSATINELLKLINSNLEFVSIYLSESHIIFTFGTTTLYSR
CCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEEECCEEEEEECHHHHHHH
LIEGKYPNISTLIPNEFQTVINIDRQRMLQGVDRSSLLASEWANNNVNLEIVNESTIQIS
HHCCCCCCCCEECCCHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCEEEEE
SNASQIGKISETQQIDVIQGKKQLNISFDGRFMLDALRAIKEETVTLSFSGSMRPILIEA
CCHHHHCCCCCCCEEEEECCCEEEEEEECCHHHHHHHHHHHHCEEEEEECCCCCEEEEEC
GTQSAAIHLISPVRAY
CCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]