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The map label for this gene is dacA [H]

Identifier: 118477901

GI number: 118477901

Start: 2403828

End: 2405180

Strand: Direct

Name: dacA [H]

Synonym: BALH_2246

Alternate gene names: 118477901

Gene position: 2403828-2405180 (Clockwise)

Preceding gene: 118477900

Following gene: 118477902

Centisome position: 45.73

GC content: 33.11

Gene sequence:

>1353_bases
TTGATAACGTTGTCTATTTTTGAAGGATTATGTCAGAATAACGCTTTTTTGATTACAAAGATATTGGAGGTAACAGAAGT
GAAAGGTATGTTTTGCAAGAGATTTATTGCAATAATAACAGTGCTTACACTATTTTGTAGCATAACTGTTATATCTGGAA
GTGCATCAGCGGAAACAGTTTCTGCTATAGAAGTAGAAGCAGGATCAGCAGTTTTAGTAGAAGCAAATTCCGGGAAAATT
TTATATGAAAAAAATGCAGATGAATTATTAGCAATTGCTAGTATGACAAAAATGATGAGTGAATATTTAGTTCATGAAGC
GGTGGAGAACGGAAAACTTAAATGGAATCAAAAAGTTAAAATCTCTGAATATGCATATAAGATTTCGCAAGATCGTTCCT
TATCAAACGTTCCATTAGAAAATGGAGGCTCTTATACGGTCAAAGAGTTATATGAGGCAATGGTAATTTATTCTGCAAAC
GGTGCAACAATTGCTTTAGCTGAAGAGATTGCTGGAAAAGAAGTTAATTTCGTAAAAATGATGAACGATAAGTCAAAAGA
GTTTGGAATGAAAAATTATAAATTTGTAAACTCAACAGGTTTAACAAACCATGATTTAAAAGGACATCACCCAGAAGGGA
CAACTCCAGACGAGAAAAATAAAATGTCCGCAAGAGATTGTGCGATTTTAGCACAACGTCTTATTCAAGATTTTCCGAAA
ATATTAGATACTGCAAAAATCCCTAAAAAAACATTTCAACAAGGTGGTAAGTATCCAATTGATATGACGAACTTTAACTG
GATGTTAAAAGGTTTAATTAAGCAATACGAAGGTGTAGATGGGTTGAAAACAGGAACTACCCCTGAAGCTGGCGATTGTT
TCACTGGTACAGCAGAAAGAAACGGTATGCGTTTAATCTCTGTCGTAATAAAAGCAAATTCTCATGCAGCACGTTTTGAT
GAAACGAAGAAATTATATGATTATGGATTTGCGAATTTTGAAGTGAGGAAAGTTAATGAAAAGGATTCAGTAGTAAACGG
ACATGAAACAGTGCGAATAGCAAATGCGAAAGACAAAGATGTAGTTGTTCAAACGAAGCAAGCTGTTTCACTTCCAATAC
TAAAGAGCAACAAAGACGTATATAAAAAAGAATTTAAAGTATTGAATAAAGAACAAGAAGCACCTATTAAAAAAGGTGTA
ACAATTAGTAAAATGATTATCTCGCCTAAAGATAGTAAAGATCCTGGGTTTTTATTAGGTAAATCATTACAAGTAGACCT
TGTAACAAAATCTGATATAGAACAAGCGAATTGGTTAACAAGTTTTATGCGTAACATCGGATCTTTTTTTTAG

Upstream 100 bases:

>100_bases
TTTATTTTTTGTGAATATGGAACAAATGTATTATTTAGATTGTCTATTAAGTGTAATCCTAAAAAATATGAAAGTGGTCC
TAATGTCTTATATTTAGAAT

Downstream 100 bases:

>100_bases
CGGTATGTGGGATAGTGCGTTTGATATAGTAAAAAGCTAATTTTATGTGGATAGCTTGGAATTGAAGAGGCATTAATTTT
CGAATTTTTCCCGTCGGAAC

Product: D-alanyl-D-alanine carboxypeptidase A

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MITLSIFEGLCQNNAFLITKILEVTEVKGMFCKRFIAIITVLTLFCSITVISGSASAETVSAIEVEAGSAVLVEANSGKI
LYEKNADELLAIASMTKMMSEYLVHEAVENGKLKWNQKVKISEYAYKISQDRSLSNVPLENGGSYTVKELYEAMVIYSAN
GATIALAEEIAGKEVNFVKMMNDKSKEFGMKNYKFVNSTGLTNHDLKGHHPEGTTPDEKNKMSARDCAILAQRLIQDFPK
ILDTAKIPKKTFQQGGKYPIDMTNFNWMLKGLIKQYEGVDGLKTGTTPEAGDCFTGTAERNGMRLISVVIKANSHAARFD
ETKKLYDYGFANFEVRKVNEKDSVVNGHETVRIANAKDKDVVVQTKQAVSLPILKSNKDVYKKEFKVLNKEQEAPIKKGV
TISKMIISPKDSKDPGFLLGKSLQVDLVTKSDIEQANWLTSFMRNIGSFF

Sequences:

>Translated_450_residues
MITLSIFEGLCQNNAFLITKILEVTEVKGMFCKRFIAIITVLTLFCSITVISGSASAETVSAIEVEAGSAVLVEANSGKI
LYEKNADELLAIASMTKMMSEYLVHEAVENGKLKWNQKVKISEYAYKISQDRSLSNVPLENGGSYTVKELYEAMVIYSAN
GATIALAEEIAGKEVNFVKMMNDKSKEFGMKNYKFVNSTGLTNHDLKGHHPEGTTPDEKNKMSARDCAILAQRLIQDFPK
ILDTAKIPKKTFQQGGKYPIDMTNFNWMLKGLIKQYEGVDGLKTGTTPEAGDCFTGTAERNGMRLISVVIKANSHAARFD
ETKKLYDYGFANFEVRKVNEKDSVVNGHETVRIANAKDKDVVVQTKQAVSLPILKSNKDVYKKEFKVLNKEQEAPIKKGV
TISKMIISPKDSKDPGFLLGKSLQVDLVTKSDIEQANWLTSFMRNIGSFF
>Mature_450_residues
MITLSIFEGLCQNNAFLITKILEVTEVKGMFCKRFIAIITVLTLFCSITVISGSASAETVSAIEVEAGSAVLVEANSGKI
LYEKNADELLAIASMTKMMSEYLVHEAVENGKLKWNQKVKISEYAYKISQDRSLSNVPLENGGSYTVKELYEAMVIYSAN
GATIALAEEIAGKEVNFVKMMNDKSKEFGMKNYKFVNSTGLTNHDLKGHHPEGTTPDEKNKMSARDCAILAQRLIQDFPK
ILDTAKIPKKTFQQGGKYPIDMTNFNWMLKGLIKQYEGVDGLKTGTTPEAGDCFTGTAERNGMRLISVVIKANSHAARFD
ETKKLYDYGFANFEVRKVNEKDSVVNGHETVRIANAKDKDVVVQTKQAVSLPILKSNKDVYKKEFKVLNKEQEAPIKKGV
TISKMIISPKDSKDPGFLLGKSLQVDLVTKSDIEQANWLTSFMRNIGSFF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=407, Percent_Identity=30.7125307125307, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1786851, Length=382, Percent_Identity=29.0575916230367, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI87082022, Length=389, Percent_Identity=27.5064267352185, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87082054, Length=274, Percent_Identity=27.007299270073, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 49965; Mature: 49965

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLSIFEGLCQNNAFLITKILEVTEVKGMFCKRFIAIITVLTLFCSITVISGSASAETV
CEEHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEE
SAIEVEAGSAVLVEANSGKILYEKNADELLAIASMTKMMSEYLVHEAVENGKLKWNQKVK
EEEEEECCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEE
ISEYAYKISQDRSLSNVPLENGGSYTVKELYEAMVIYSANGATIALAEEIAGKEVNFVKM
HHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHEEEEECCCCCEEEEEHHHCCCCEEEEEE
MNDKSKEFGMKNYKFVNSTGLTNHDLKGHHPEGTTPDEKNKMSARDCAILAQRLIQDFPK
CCCCHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ILDTAKIPKKTFQQGGKYPIDMTNFNWMLKGLIKQYEGVDGLKTGTTPEAGDCFTGTAER
HHHHHCCCHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
NGMRLISVVIKANSHAARFDETKKLYDYGFANFEVRKVNEKDSVVNGHETVRIANAKDKD
CCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCC
VVVQTKQAVSLPILKSNKDVYKKEFKVLNKEQEAPIKKGVTISKMIISPKDSKDPGFLLG
EEEEEHHHHCCCEEECCCHHHHHHHHHHCCHHCCHHHHCCEEEEEEECCCCCCCCCEEEC
KSLQVDLVTKSDIEQANWLTSFMRNIGSFF
CCEEEEEEECCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MITLSIFEGLCQNNAFLITKILEVTEVKGMFCKRFIAIITVLTLFCSITVISGSASAETV
CEEHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEE
SAIEVEAGSAVLVEANSGKILYEKNADELLAIASMTKMMSEYLVHEAVENGKLKWNQKVK
EEEEEECCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEE
ISEYAYKISQDRSLSNVPLENGGSYTVKELYEAMVIYSANGATIALAEEIAGKEVNFVKM
HHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHEEEEECCCCCEEEEEHHHCCCCEEEEEE
MNDKSKEFGMKNYKFVNSTGLTNHDLKGHHPEGTTPDEKNKMSARDCAILAQRLIQDFPK
CCCCHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ILDTAKIPKKTFQQGGKYPIDMTNFNWMLKGLIKQYEGVDGLKTGTTPEAGDCFTGTAER
HHHHHCCCHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
NGMRLISVVIKANSHAARFDETKKLYDYGFANFEVRKVNEKDSVVNGHETVRIANAKDKD
CCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCC
VVVQTKQAVSLPILKSNKDVYKKEFKVLNKEQEAPIKKGVTISKMIISPKDSKDPGFLLG
EEEEEHHHHCCCEEECCCHHHHHHHHHHCCHHCCHHHHCCEEEEEEECCCCCCCCCEEEC
KSLQVDLVTKSDIEQANWLTSFMRNIGSFF
CCEEEEEEECCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]