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The map label for this gene is 118477510

Identifier: 118477510

GI number: 118477510

Start: 1987672

End: 1989216

Strand: Direct

Name: 118477510

Synonym: BALH_1835

Alternate gene names: NA

Gene position: 1987672-1989216 (Clockwise)

Preceding gene: 118477509

Following gene: 118477511

Centisome position: 37.81

GC content: 37.22

Gene sequence:

>1545_bases
TTGAAAATTCAGTCATCTAGTGAAATTTCTAAACTTTTTAGTATATTATTATTGGAAATTGGAGGGGAAAGAATGGCAAT
TAGAACAGGGGAACAGTATATTGAAGGGTTGCGAAAAAGAAATCCTGAAATTTGGATTGGAGGAAGAAAAGTAACGGATG
TAGTAAATGAAGATGTTTTTCGTGAACCAATTATACAAATTGCGAACTTATATGATATGCAGCACAATCCAGAATATCAA
GATAAGATTACTCATATATGCGAAGAGACAGGAGAAAGGGTATCAAATGCATTTTTAGTACCGAGAAATTATGAGGATTT
AAAAGTACGCCGCGAATTATTTGAAGTGTGGGCAAAAGCCACTTATGGTTTAATGGGTAGAACACCTGATTTTTTAAATG
TAACGGTTACTTCAATGGCAAGTAATTCGTGGTTTTTTGAGAAGTTTAATCCAGAATGGGGAGCGAATATAAAAAATTAT
TATAAACATATAAGGGATAATGATTTGTTTTTAACACACGCAATTATTAATCCGCAAAATGATCGGAGTAAGGCCTCCCA
TCAACAAGAAGATGAAACAATGCATTTAGGGGTAGTAAGTGAAACAGCGGAAGGAATGTATGTTAGTGGTGCTAAAATGT
TAGCGACACTGGCGCCGATTACGGATGAGGTAATAATATATTCCTATCCAAGTTTTAAGCCAGGTGATGAACGTCATGCA
ATTTCTTTTGCAGTTCCAATTGACGCACCAGGTCTAAGAATTCTATGTCGTGAGCCAATGCAAGATGGAAAACGATCTTT
ATTTGATCACCCACTTGCATCAAGGTTTGAGGAAATGGATGCATTACTAGTATTTCATAATGTGTTTATCCCGTGGGATA
AAGTATTTATTTATCAAAGTGTGGAAGCTGGGAATCAATTGTATCCTAAAACCGGAATTGGTCATCAACCAGCACATCAA
TCTGGGGTAAGAGGGTTAGTAAAACTATCTTTTGCAGCTGAAGTAGCTATGAAATTAGCAGATTCGATTGGAGTAGATGG
TTTTTTAAATGTACAAAACCAGTTAGCAGAGCTTATGCAAGATGTCGAAACGATACGTGCTCTCCTGCAAGTAGCTGAAT
ATGAGTATGAAACGAATTCATATGGAGAAGTTATTCCTGCGAGGTTACCGCTTGATACGATACGCAGTTTATTACCTAAG
ATGTATCCGCGGGCTATTGAGATTATTCAAACTATAGGTGCGGGTGGATTATTAATGTCACCGACAGGTGCTGATTTTAT
GAACCCCGAGATTCATGATGATATGCAAAAGTATTATATCGGGCGTGAAGGAGTATCCTCTGAGGAACGGGTTCGTTTGT
TTAAATTAGCATGGGACTTATGTGGTGAGGCTTTTGGACAACGGTTATTACAATATGAGCGTTATTATTCTGGAGATCCG
GTGCGTAAAATGGGAATGTTTTATAATGCGTACAAAAAACAACAAACTTTTTCACTTGTGAACAATGCGTTGCAATCGAG
TTCATCTGAAGGGGCCTTAAGTTAA

Upstream 100 bases:

>100_bases
GGACGGGGGACCGTTCCTTACTCATTTACACTAAAAAATTTTTAAGATTTCATAATATGAAATAAAGGAGAATCTGTAAG
AAATAGAATTGATATAATAA

Downstream 100 bases:

>100_bases
ATAAAAATGTTTGCGCTTACATTTTTTATTTTGAAAAGGAAGGAGCAATAGATGATAGAAAGAATTTTACTGTTAGGATG
TAATTCTTCTTATATTTTTA

Product: 4-hydroxyphenylacetate-3-hydroxylase

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-hydroxylase; 4-HPA 3-monooxygenase large component [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKIQSSSEISKLFSILLLEIGGERMAIRTGEQYIEGLRKRNPEIWIGGRKVTDVVNEDVFREPIIQIANLYDMQHNPEYQ
DKITHICEETGERVSNAFLVPRNYEDLKVRRELFEVWAKATYGLMGRTPDFLNVTVTSMASNSWFFEKFNPEWGANIKNY
YKHIRDNDLFLTHAIINPQNDRSKASHQQEDETMHLGVVSETAEGMYVSGAKMLATLAPITDEVIIYSYPSFKPGDERHA
ISFAVPIDAPGLRILCREPMQDGKRSLFDHPLASRFEEMDALLVFHNVFIPWDKVFIYQSVEAGNQLYPKTGIGHQPAHQ
SGVRGLVKLSFAAEVAMKLADSIGVDGFLNVQNQLAELMQDVETIRALLQVAEYEYETNSYGEVIPARLPLDTIRSLLPK
MYPRAIEIIQTIGAGGLLMSPTGADFMNPEIHDDMQKYYIGREGVSSEERVRLFKLAWDLCGEAFGQRLLQYERYYSGDP
VRKMGMFYNAYKKQQTFSLVNNALQSSSSEGALS

Sequences:

>Translated_514_residues
MKIQSSSEISKLFSILLLEIGGERMAIRTGEQYIEGLRKRNPEIWIGGRKVTDVVNEDVFREPIIQIANLYDMQHNPEYQ
DKITHICEETGERVSNAFLVPRNYEDLKVRRELFEVWAKATYGLMGRTPDFLNVTVTSMASNSWFFEKFNPEWGANIKNY
YKHIRDNDLFLTHAIINPQNDRSKASHQQEDETMHLGVVSETAEGMYVSGAKMLATLAPITDEVIIYSYPSFKPGDERHA
ISFAVPIDAPGLRILCREPMQDGKRSLFDHPLASRFEEMDALLVFHNVFIPWDKVFIYQSVEAGNQLYPKTGIGHQPAHQ
SGVRGLVKLSFAAEVAMKLADSIGVDGFLNVQNQLAELMQDVETIRALLQVAEYEYETNSYGEVIPARLPLDTIRSLLPK
MYPRAIEIIQTIGAGGLLMSPTGADFMNPEIHDDMQKYYIGREGVSSEERVRLFKLAWDLCGEAFGQRLLQYERYYSGDP
VRKMGMFYNAYKKQQTFSLVNNALQSSSSEGALS
>Mature_514_residues
MKIQSSSEISKLFSILLLEIGGERMAIRTGEQYIEGLRKRNPEIWIGGRKVTDVVNEDVFREPIIQIANLYDMQHNPEYQ
DKITHICEETGERVSNAFLVPRNYEDLKVRRELFEVWAKATYGLMGRTPDFLNVTVTSMASNSWFFEKFNPEWGANIKNY
YKHIRDNDLFLTHAIINPQNDRSKASHQQEDETMHLGVVSETAEGMYVSGAKMLATLAPITDEVIIYSYPSFKPGDERHA
ISFAVPIDAPGLRILCREPMQDGKRSLFDHPLASRFEEMDALLVFHNVFIPWDKVFIYQSVEAGNQLYPKTGIGHQPAHQ
SGVRGLVKLSFAAEVAMKLADSIGVDGFLNVQNQLAELMQDVETIRALLQVAEYEYETNSYGEVIPARLPLDTIRSLLPK
MYPRAIEIIQTIGAGGLLMSPTGADFMNPEIHDDMQKYYIGREGVSSEERVRLFKLAWDLCGEAFGQRLLQYERYYSGDP
VRKMGMFYNAYKKQQTFSLVNNALQSSSSEGALS

Specific function: Utilizes FADH(2) supplied by hpaC, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA) [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012687 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: 1.14.13.3

Molecular weight: Translated: 58419; Mature: 58419

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQSSSEISKLFSILLLEIGGERMAIRTGEQYIEGLRKRNPEIWIGGRKVTDVVNEDVF
CCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
REPIIQIANLYDMQHNPEYQDKITHICEETGERVSNAFLVPRNYEDLKVRRELFEVWAKA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCEEECCCCCHHHHHHHHHHHHHHHH
TYGLMGRTPDFLNVTVTSMASNSWFFEKFNPEWGANIKNYYKHIRDNDLFLTHAIINPQN
HHHHCCCCCCHHEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEECCCC
DRSKASHQQEDETMHLGVVSETAEGMYVSGAKMLATLAPITDEVIIYSYPSFKPGDERHA
CHHHHHCCCCCCEEEEEEEHHCCCCEEECCHHHHHHHHCCCCCEEEEECCCCCCCCCCCE
ISFAVPIDAPGLRILCREPMQDGKRSLFDHPLASRFEEMDALLVFHNVFIPWDKVFIYQS
EEEEEECCCCCCCCEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
VEAGNQLYPKTGIGHQPAHQSGVRGLVKLSFAAEVAMKLADSIGVDGFLNVQNQLAELMQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHH
DVETIRALLQVAEYEYETNSYGEVIPARLPLDTIRSLLPKMYPRAIEIIQTIGAGGLLMS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
PTGADFMNPEIHDDMQKYYIGREGVSSEERVRLFKLAWDLCGEAFGQRLLQYERYYSGDP
CCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VRKMGMFYNAYKKQQTFSLVNNALQSSSSEGALS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKIQSSSEISKLFSILLLEIGGERMAIRTGEQYIEGLRKRNPEIWIGGRKVTDVVNEDVF
CCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
REPIIQIANLYDMQHNPEYQDKITHICEETGERVSNAFLVPRNYEDLKVRRELFEVWAKA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCEEECCCCCHHHHHHHHHHHHHHHH
TYGLMGRTPDFLNVTVTSMASNSWFFEKFNPEWGANIKNYYKHIRDNDLFLTHAIINPQN
HHHHCCCCCCHHEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEECCCC
DRSKASHQQEDETMHLGVVSETAEGMYVSGAKMLATLAPITDEVIIYSYPSFKPGDERHA
CHHHHHCCCCCCEEEEEEEHHCCCCEEECCHHHHHHHHCCCCCEEEEECCCCCCCCCCCE
ISFAVPIDAPGLRILCREPMQDGKRSLFDHPLASRFEEMDALLVFHNVFIPWDKVFIYQS
EEEEEECCCCCCCCEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
VEAGNQLYPKTGIGHQPAHQSGVRGLVKLSFAAEVAMKLADSIGVDGFLNVQNQLAELMQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHH
DVETIRALLQVAEYEYETNSYGEVIPARLPLDTIRSLLPKMYPRAIEIIQTIGAGGLLMS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
PTGADFMNPEIHDDMQKYYIGREGVSSEERVRLFKLAWDLCGEAFGQRLLQYERYYSGDP
CCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VRKMGMFYNAYKKQQTFSLVNNALQSSSSEGALS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA