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The map label for this gene is topB [H]

Identifier: 118477367

GI number: 118477367

Start: 1837803

End: 1839947

Strand: Direct

Name: topB [H]

Synonym: BALH_1686

Alternate gene names: 118477367

Gene position: 1837803-1839947 (Clockwise)

Preceding gene: 118477365

Following gene: 118477368

Centisome position: 34.96

GC content: 37.3

Gene sequence:

>2145_bases
ATGAAATTAATTATTGCCGAGAAACCAGATCAAGGTTTGGCTCTTGTTTCACAATTTAAATATCGCCGGAAAGATGGATA
TTTAGAAGTAGAAGCGAATGAGTTATTTCCAAATGGAGCGTACTGTACATGGGCAATTGGTCATTTGACGCAGTTATGTA
ATCCAGAGCATTATCACGCAGAGTGGAAAAAATGGTCACTTAATACGTTACCAATGATTCCAGAGCGTTTTCAATTTGAA
GTAACAAAGTCAAAGTATAAGCAATTTAACGTTGTGAAACAGCTGTTACATAATCCGCAAGTAACAGAAATTATTCACGC
GGGCGATGCCGGGCGTGAAGGGGAATTGATTGTACGAAATATTATTAACCTTTGCAACGTGCAAAAGCCGATGAAACGTC
TATGGATTTCATCTTTAACGAAGCAAGCTATTTATCAAGGTTTTAAAAATTTACTGGATGAATCAGATACAATCAATACG
TATTACGAAGCATATACAAGATCGTGCGCTGACTGGGTCGTTGGTATGAATGCATCGCGTGTCTTTAGTATTTTGCTAAA
GAAAAAAGGAATGAACGATGTATTTTCAGCGGGTCGTGTACAAACGCCGACACTCGCATTAATCGTAAAGCGTGAAAAAG
AAATAGAAAACTTTAAGTCTGAGCCGTTTTGGGAAGTGTTCGCAACCTTTAATATAGAAGGAAAGAAGTATGAGGGGAAA
TGGGAGAAGGACAATGAATCTCGCCTAAAAGACCCTGATATGGCAAATAAAATTGCGGCATTTTGCCAAGGGAAACCAGC
TGAAGTAAAGGAAATGAAAACGGAGCGTAAAGAGTTTCAGCCACCGCTTTTATTTAACTTATCTTCACTGCAAGCTACGG
CAAATAAAGCATTCAAATTTTCACCGAAAAAGACACTTGATATAACGCAAGCATTATATCAAAAAGGGATTGTCTCTTAT
CCTCGTTCAGATTCCAACTATGTGACACAAGGAGAGGCAGCAACGTTCCCTGATATTTTACAGAAGTTAAGTCAGTTCGA
TGAATATAAAGGGTTATTACCAGCACCAGTTGAATCCATTATGAACAATAAGCGTTATGTGAATGAAAAGAAAGTAACAG
ATCACTACGCTATTATTCCGACAGAGCAAGTTACAAACCCAAGCAGATTATCAGGTGATGAAAAGAAAATTTACGATATG
ATTGTAAGGAGACTTATTGCGGCTCACTATGAAGTTGCAATCTTTGATTATACGACGATTATAACGCTTGTAGATGAACG
TGCTGAATTCATTTCAAAAGGAAAACAGCAAATTCAAGAAGGTTGGCGTAAAGTTATTTTCCAAGATGATAAAGATGATG
AAACAATTCTTCCGATTGTAGTTGAAGGTGAACAAGGGAAAGTTGTAAAGGTGAAAGTGAAAGAAGGAAAAACACAGCCA
CCGAAGCGTTATACAGAAGGACAACTTATTACGTTAATGAAAACGGCAGGTAAGTATTTGGAGAACGAAGAACTTGAGAA
AGTATTAAAGAAAACAGAAGGTTTAGGTACAGAAGCGACTCGCGCTGGAATTATTACGATGCTGAAAGACCGTAAATATA
TAGATGTGAAGAAGAACCAAGTGTATGCAACCGATAAAGGGAAAGTATTAATTACCGCAATCGGAGACAAAATACTAGCT
TCACCAGAAATGACAGCGAAATGGGAGCAGCGTCTTGCAGAAATTGGCGAAGGCACAGCATCACCGGCTACATTTATGGA
ACAAACGAAAAAGCTATCAGCAAAAATTATTGAAGATGCAGTGGAAATGTCGGAAAAATGGGATTTCACTGGATTACATG
TTGAGTCGATAGAGCGAAAAGGATCGAAATTTACAACGGGTAAAAAGGTTGGTAGCTGTAAAAAATGTGATGGCGATGTA
ATTGATAAGTCAACGTTTTACGGCTGTTCTAATTACAACACGACACAATGTGACTTTACCATCTCGAAGAAGATATTAAG
TAAAACAATTTCGCAAAAGAATATGACAAAGCTCTTAAAAGGTGAAAAGACCGATTTAATTAAAGGCTTTAAAAAGGGCG
AGAAAACATTTGATGCGAAGTTAGAGTGGAAAGATAATAAGATTAATTTTGTATTTGAGAATTAA

Upstream 100 bases:

>100_bases
GGGATAACCCGCGAATGATAAATAAGATTGTTGTTTTATCATCGCTTCTAAACATATAGACAACGAAAGTAACACTGGAA
TACAGATAGGGAGTTATACA

Downstream 100 bases:

>100_bases
GTGGTGTCAACAAATAAAAAAATATGAAAATTAAACTGTATCCTATAGAATAGACACTTAAAAAAGTCTATTCTATAGGA
TATTTTTTTGAATAAGTAGT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHAEWKKWSLNTLPMIPERFQFE
VTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRNIINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINT
YYEAYTRSCADWVVGMNASRVFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYEGK
WEKDNESRLKDPDMANKIAAFCQGKPAEVKEMKTERKEFQPPLLFNLSSLQATANKAFKFSPKKTLDITQALYQKGIVSY
PRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESIMNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDM
IVRRLIAAHYEVAIFDYTTIITLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVVEGEQGKVVKVKVKEGKTQP
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQVYATDKGKVLITAIGDKILA
SPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDAVEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDV
IDKSTFYGCSNYNTTQCDFTISKKILSKTISQKNMTKLLKGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN

Sequences:

>Translated_714_residues
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHAEWKKWSLNTLPMIPERFQFE
VTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRNIINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINT
YYEAYTRSCADWVVGMNASRVFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYEGK
WEKDNESRLKDPDMANKIAAFCQGKPAEVKEMKTERKEFQPPLLFNLSSLQATANKAFKFSPKKTLDITQALYQKGIVSY
PRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESIMNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDM
IVRRLIAAHYEVAIFDYTTIITLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVVEGEQGKVVKVKVKEGKTQP
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQVYATDKGKVLITAIGDKILA
SPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDAVEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDV
IDKSTFYGCSNYNTTQCDFTISKKILSKTISQKNMTKLLKGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN
>Mature_714_residues
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHAEWKKWSLNTLPMIPERFQFE
VTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRNIINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINT
YYEAYTRSCADWVVGMNASRVFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYEGK
WEKDNESRLKDPDMANKIAAFCQGKPAEVKEMKTERKEFQPPLLFNLSSLQATANKAFKFSPKKTLDITQALYQKGIVSY
PRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESIMNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDM
IVRRLIAAHYEVAIFDYTTIITLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVVEGEQGKVVKVKVKEGKTQP
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQVYATDKGKVLITAIGDKILA
SPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDAVEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDV
IDKSTFYGCSNYNTTQCDFTISKKILSKTISQKNMTKLLKGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=674, Percent_Identity=24.7774480712166, Blast_Score=143, Evalue=5e-34,
Organism=Homo sapiens, GI4507635, Length=458, Percent_Identity=25.764192139738, Blast_Score=107, Evalue=4e-23,
Organism=Escherichia coli, GI1788061, Length=619, Percent_Identity=33.6025848142165, Blast_Score=357, Evalue=2e-99,
Organism=Escherichia coli, GI1787529, Length=518, Percent_Identity=28.1853281853282, Blast_Score=135, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=626, Percent_Identity=27.7955271565495, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI32563869, Length=500, Percent_Identity=25.6, Blast_Score=119, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=629, Percent_Identity=25.7551669316375, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24585251, Length=702, Percent_Identity=24.7863247863248, Blast_Score=140, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24640096, Length=493, Percent_Identity=24.3407707910751, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 81588; Mature: 81588

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHA
CEEEEECCCCCCHHHHHHHHHHHCCCCEEEEHHHCCCCCCEEEHHHHHHHHHCCCCHHCC
EWKKWSLNTLPMIPERFQFEVTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRN
CHHCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCHHHHH
IINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINTYYEAYTRSCADWVVGMNASR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHH
VFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYEGK
HHHHHHHHCCCHHHHHCCCCCCCEEEEEEECHHHHHHCCCCCCEEEEEEEEECCCEECCC
WEKDNESRLKDPDMANKIAAFCQGKPAEVKEMKTERKEFQPPLLFNLSSLQATANKAFKF
CCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCEEC
SPKKTLDITQALYQKGIVSYPRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESI
CCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
MNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDMIVRRLIAAHYEVAIFDYTTI
HCCCHHCCCCCCCCCEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEHHHH
ITLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVVEGEQGKVVKVKVKEGKTQP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCC
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQ
CCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHEECCCCEEEEECCE
VYATDKGKVLITAIGDKILASPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDA
EEEECCCCEEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDVIDKSTFYGCSNYNTTQCDFT
HHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEECHH
ISKKILSKTISQKNMTKLLKGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEECCEEEEEECC
>Mature Secondary Structure
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHA
CEEEEECCCCCCHHHHHHHHHHHCCCCEEEEHHHCCCCCCEEEHHHHHHHHHCCCCHHCC
EWKKWSLNTLPMIPERFQFEVTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRN
CHHCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCHHHHH
IINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINTYYEAYTRSCADWVVGMNASR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHH
VFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYEGK
HHHHHHHHCCCHHHHHCCCCCCCEEEEEEECHHHHHHCCCCCCEEEEEEEEECCCEECCC
WEKDNESRLKDPDMANKIAAFCQGKPAEVKEMKTERKEFQPPLLFNLSSLQATANKAFKF
CCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCEEC
SPKKTLDITQALYQKGIVSYPRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESI
CCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
MNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDMIVRRLIAAHYEVAIFDYTTI
HCCCHHCCCCCCCCCEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEHHHH
ITLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVVEGEQGKVVKVKVKEGKTQP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCC
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQ
CCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHEECCCCEEEEECCE
VYATDKGKVLITAIGDKILASPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDA
EEEECCCCEEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDVIDKSTFYGCSNYNTTQCDFT
HHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEECHH
ISKKILSKTISQKNMTKLLKGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1329032 [H]