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The map label for this gene is purB [H]

Identifier: 118476041

GI number: 118476041

Start: 326559

End: 327902

Strand: Direct

Name: purB [H]

Synonym: BALH_0284

Alternate gene names: 118476041

Gene position: 326559-327902 (Clockwise)

Preceding gene: 118476040

Following gene: 118476042

Centisome position: 6.21

GC content: 37.87

Gene sequence:

>1344_bases
TTGGGACCATCAAGAACAACTGTTGGAGGGAAAAAGATGATTAGTCGTTATACACGCCCAGAAATGGGTGCGATTTGGAC
GGAAGAGAACAAATTTAAAGCGTGGTTAGAAGTTGAGATTTTAGCTTGTGAAGCATGGGCTGAGCTTGGCGATATTCCTA
AAGAAGATGTTAAGAAAATTCGTGAGCATGCATCATTTGATATTGATCGTATTTATGAAATTGAAAAAGAGACACGTCAT
GACGTAGTTGCATTCACTCGTGCTGTATCAGAAACACCAGCATTAGGTGAGGAACGTAAATGGGTTCATTACGGTTTAAC
ATCTACAGACGTAGTAGATACAGCGTTATCTTACATCTTAAAACAAGCGAATGAAATCATATTAAAAGACTTAGAAAACT
TCGTAAGCATTTTAGCTAACAAAGCGAAAGAGCATAAATACACGATTATGATGGGAAGAACACACGGTGTCCATGCAGAA
CCAACAACATTTGGTCTAAAACTTGGTCTTTGGTATGAAGAAATGAAACGTAACGTAGAGCGTTTCAAACAAGCTGCTAA
TACAGTTCGTGTTGGTAAACTATCTGGTGCGGTTGGTACATATGCAAATATTGATCCATTCGTAGAAAAATATGTTTGTG
AAAATTTAGGATTAGAAGCAGCACCAATTTCAACACAAACATTGCAACGTGATCGCCATGCTCATTACATGTCAACACTT
GCATTAATCGCAACATCTATCGAAAAGATGGCAGTTGAGATTCGTGGTTTACAAAAGAGTGAAACACGTGAAGTTGAAGA
AGCTTTCGCAAAAGGTCAAAAAGGTTCTTCTGCAATGCCACATAAGCGTAATCCAATTGGATCTGAAAATATGACTGGTT
TAGCTCGTGTTATCCGCGGTTATATGATGACAGCTTATGAGAATGTTCCATTATGGCATGAGCGTGATATTTCTCACTCT
TCAGCAGAACGCGTAATTTTACCAGATGCTACAATCGCGTTAAATTACATGCTAAATCGCTTTGGTAATATCGTTAAAAA
CTTAACTGTATACCCAGAGAATATGAAACGCAATATGACAAGAACATACGGCTTAATTTATTCTCAGCGCGTAATGCTTA
CATTAATCGATAAAGGTATGGTGCGTGAAGAAGCTTACGATATCGTACAACCTAAAGCGATGGAAGCTTGGGAAACACAA
GTACAATTTAAAGAGCTTGTAGAAGCTGATGAGCGTATTACGAGCAAGTTAACACAAGAAGAAATTAATGAATGCTTTAA
CTATGAGCATCATATGCAACACGTTGATACAATCTTTGAACGCCTAGGATTAAACGAAGCGTAA

Upstream 100 bases:

>100_bases
ATGGAAAAGAAGAAGCAAAAGCGCAGCGAAAAATGGGGCATGTTAATATTTTAAATGATAATATTGAAGTCGCTCTAGAA
AAAGCAAAGAGTTTGCATAT

Downstream 100 bases:

>100_bases
AATTTCATATGGCACAGAATAGAGTCATTCTGTGCCATTCTTTATAAAAACATTATTCACATTTTTCAAACTACAGGGGG
CTTGCGAAATGCAAAAGCTA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MGPSRTTVGGKKMISRYTRPEMGAIWTEENKFKAWLEVEILACEAWAELGDIPKEDVKKIREHASFDIDRIYEIEKETRH
DVVAFTRAVSETPALGEERKWVHYGLTSTDVVDTALSYILKQANEIILKDLENFVSILANKAKEHKYTIMMGRTHGVHAE
PTTFGLKLGLWYEEMKRNVERFKQAANTVRVGKLSGAVGTYANIDPFVEKYVCENLGLEAAPISTQTLQRDRHAHYMSTL
ALIATSIEKMAVEIRGLQKSETREVEEAFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGYMMTAYENVPLWHERDISHS
SAERVILPDATIALNYMLNRFGNIVKNLTVYPENMKRNMTRTYGLIYSQRVMLTLIDKGMVREEAYDIVQPKAMEAWETQ
VQFKELVEADERITSKLTQEEINECFNYEHHMQHVDTIFERLGLNEA

Sequences:

>Translated_447_residues
MGPSRTTVGGKKMISRYTRPEMGAIWTEENKFKAWLEVEILACEAWAELGDIPKEDVKKIREHASFDIDRIYEIEKETRH
DVVAFTRAVSETPALGEERKWVHYGLTSTDVVDTALSYILKQANEIILKDLENFVSILANKAKEHKYTIMMGRTHGVHAE
PTTFGLKLGLWYEEMKRNVERFKQAANTVRVGKLSGAVGTYANIDPFVEKYVCENLGLEAAPISTQTLQRDRHAHYMSTL
ALIATSIEKMAVEIRGLQKSETREVEEAFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGYMMTAYENVPLWHERDISHS
SAERVILPDATIALNYMLNRFGNIVKNLTVYPENMKRNMTRTYGLIYSQRVMLTLIDKGMVREEAYDIVQPKAMEAWETQ
VQFKELVEADERITSKLTQEEINECFNYEHHMQHVDTIFERLGLNEA
>Mature_446_residues
GPSRTTVGGKKMISRYTRPEMGAIWTEENKFKAWLEVEILACEAWAELGDIPKEDVKKIREHASFDIDRIYEIEKETRHD
VVAFTRAVSETPALGEERKWVHYGLTSTDVVDTALSYILKQANEIILKDLENFVSILANKAKEHKYTIMMGRTHGVHAEP
TTFGLKLGLWYEEMKRNVERFKQAANTVRVGKLSGAVGTYANIDPFVEKYVCENLGLEAAPISTQTLQRDRHAHYMSTLA
LIATSIEKMAVEIRGLQKSETREVEEAFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGYMMTAYENVPLWHERDISHSS
AERVILPDATIALNYMLNRFGNIVKNLTVYPENMKRNMTRTYGLIYSQRVMLTLIDKGMVREEAYDIVQPKAMEAWETQV
QFKELVEADERITSKLTQEEINECFNYEHHMQHVDTIFERLGLNEA

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI183227688, Length=400, Percent_Identity=29.75, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI4557269, Length=398, Percent_Identity=29.1457286432161, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1787376, Length=305, Percent_Identity=26.8852459016393, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17508577, Length=408, Percent_Identity=26.4705882352941, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI32564234, Length=367, Percent_Identity=26.4305177111717, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323391, Length=391, Percent_Identity=29.923273657289, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24647570, Length=397, Percent_Identity=26.448362720403, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51189; Mature: 51058

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPSRTTVGGKKMISRYTRPEMGAIWTEENKFKAWLEVEILACEAWAELGDIPKEDVKKI
CCCCCCCCCHHHHHHHHCCCCCCCEECCCCCEEEEEEEEEEEHHHHHHHCCCCHHHHHHH
REHASFDIDRIYEIEKETRHDVVAFTRAVSETPALGEERKWVHYGLTSTDVVDTALSYIL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHH
KQANEIILKDLENFVSILANKAKEHKYTIMMGRTHGVHAEPTTFGLKLGLWYEEMKRNVE
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHEEEHHHHHHHHHHHH
RFKQAANTVRVGKLSGAVGTYANIDPFVEKYVCENLGLEAAPISTQTLQRDRHAHYMSTL
HHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
ALIATSIEKMAVEIRGLQKSETREVEEAFAKGQKGSSAMPHKRNPIGSENMTGLARVIRG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
YMMTAYENVPLWHERDISHSSAERVILPDATIALNYMLNRFGNIVKNLTVYPENMKRNMT
HHHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RTYGLIYSQRVMLTLIDKGMVREEAYDIVQPKAMEAWETQVQFKELVEADERITSKLTQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EINECFNYEHHMQHVDTIFERLGLNEA
HHHHHHCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GPSRTTVGGKKMISRYTRPEMGAIWTEENKFKAWLEVEILACEAWAELGDIPKEDVKKI
CCCCCCCCHHHHHHHHCCCCCCCEECCCCCEEEEEEEEEEEHHHHHHHCCCCHHHHHHH
REHASFDIDRIYEIEKETRHDVVAFTRAVSETPALGEERKWVHYGLTSTDVVDTALSYIL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHH
KQANEIILKDLENFVSILANKAKEHKYTIMMGRTHGVHAEPTTFGLKLGLWYEEMKRNVE
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHEEEHHHHHHHHHHHH
RFKQAANTVRVGKLSGAVGTYANIDPFVEKYVCENLGLEAAPISTQTLQRDRHAHYMSTL
HHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
ALIATSIEKMAVEIRGLQKSETREVEEAFAKGQKGSSAMPHKRNPIGSENMTGLARVIRG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
YMMTAYENVPLWHERDISHSSAERVILPDATIALNYMLNRFGNIVKNLTVYPENMKRNMT
HHHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RTYGLIYSQRVMLTLIDKGMVREEAYDIVQPKAMEAWETQVQFKELVEADERITSKLTQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EINECFNYEHHMQHVDTIFERLGLNEA
HHHHHHCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]