Click here to switch to the map view.

The map label for this gene is dsbA [H]

Identifier: 117920021

GI number: 117920021

Start: 1841013

End: 1841666

Strand: Direct

Name: dsbA [H]

Synonym: Shewana3_1573

Alternate gene names: 117920021

Gene position: 1841013-1841666 (Clockwise)

Preceding gene: 117920020

Following gene: 117920022

Centisome position: 37.03

GC content: 48.01

Gene sequence:

>654_bases
ATGATGCAGTTACTAATAAGAAGAGAAAATATGAAAAGCCTAACTAATTTTGTTCGACCTTTGGCGGCGCTGGCCTGTGG
ATTGGTGTTAAGTGCGAGCTTTGCACTGCAGGCGGCGAGTTTTGAAGAAGGGAAGGATTATGTCACTGTCGCTGGGATCA
ACGAAGCACAAAAGCCTGTGCTGCGTGAGTTCTTCTCCTATAACTGCCCGCATTGCTACAAGCAGGAACCCTTTGTCGCT
TCCACGGTAAAACTCTTAGGCAAGGATGTGGTCTTCGAGCGCACGCCGGTGGGCGCGGGACGTCCGGCGTGGGAGTTGAG
TCAATTAGCCTATTTTGTTGCACAAAAGCTGAAAATGACCAAGCAAGTGCACGAAGCGATTTTTAAACAAATCCATGAAA
AGGGTGAACAATTTACTCGCCCTGAACAGGTAAAAGCTTTCTTTGTGGCTCAGGGCGCGAAGGCCGATGATGTCGATGCG
GCGATGAACTCTGTCGATGCGAAGTTTACTATGATGAATTACGACTCGCAGGCGGAATTATCGGGTATTAAGGGCGTGCC
ATCATTATTAGTCAATGGCCGCTATTTAGTGACGTCTAAGGTGCATACACCTGAAGAATTGGCCGAGCTGGTCAAATTCT
TAGCCGCCAAATAA

Upstream 100 bases:

>100_bases
TGGGTGATTTAGCACCTATTGTTAACAAATGTTTAAGAACCTTAGCCATATTTTGAGGTATCTTAATGGCCTGTGCGTGT
CGCAAACGACTTACGATTAA

Downstream 100 bases:

>100_bases
AAGGCTAATCACTGTGTTTATGGCTGCGTAAACTTATACCAATCACATTAAATATCTAATCAGTTCAGGGCCTCACAGGC
ATCTCAATCCAAGGCGCATT

Product: DSBA oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MMQLLIRRENMKSLTNFVRPLAALACGLVLSASFALQAASFEEGKDYVTVAGINEAQKPVLREFFSYNCPHCYKQEPFVA
STVKLLGKDVVFERTPVGAGRPAWELSQLAYFVAQKLKMTKQVHEAIFKQIHEKGEQFTRPEQVKAFFVAQGAKADDVDA
AMNSVDAKFTMMNYDSQAELSGIKGVPSLLVNGRYLVTSKVHTPEELAELVKFLAAK

Sequences:

>Translated_217_residues
MMQLLIRRENMKSLTNFVRPLAALACGLVLSASFALQAASFEEGKDYVTVAGINEAQKPVLREFFSYNCPHCYKQEPFVA
STVKLLGKDVVFERTPVGAGRPAWELSQLAYFVAQKLKMTKQVHEAIFKQIHEKGEQFTRPEQVKAFFVAQGAKADDVDA
AMNSVDAKFTMMNYDSQAELSGIKGVPSLLVNGRYLVTSKVHTPEELAELVKFLAAK
>Mature_217_residues
MMQLLIRRENMKSLTNFVRPLAALACGLVLSASFALQAASFEEGKDYVTVAGINEAQKPVLREFFSYNCPHCYKQEPFVA
STVKLLGKDVVFERTPVGAGRPAWELSQLAYFVAQKLKMTKQVHEAIFKQIHEKGEQFTRPEQVKAFFVAQGAKADDVDA
AMNSVDAKFTMMNYDSQAELSGIKGVPSLLVNGRYLVTSKVHTPEELAELVKFLAAK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=214, Percent_Identity=29.9065420560748, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 24113; Mature: 24113

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQLLIRRENMKSLTNFVRPLAALACGLVLSASFALQAASFEEGKDYVTVAGINEAQKPV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHH
LREFFSYNCPHCYKQEPFVASTVKLLGKDVVFERTPVGAGRPAWELSQLAYFVAQKLKMT
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHH
KQVHEAIFKQIHEKGEQFTRPEQVKAFFVAQGAKADDVDAAMNSVDAKFTMMNYDSQAEL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCEEEEECCCCHHHH
SGIKGVPSLLVNGRYLVTSKVHTPEELAELVKFLAAK
HCCCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MMQLLIRRENMKSLTNFVRPLAALACGLVLSASFALQAASFEEGKDYVTVAGINEAQKPV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHH
LREFFSYNCPHCYKQEPFVASTVKLLGKDVVFERTPVGAGRPAWELSQLAYFVAQKLKMT
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHH
KQVHEAIFKQIHEKGEQFTRPEQVKAFFVAQGAKADDVDAAMNSVDAKFTMMNYDSQAEL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCEEEEECCCCHHHH
SGIKGVPSLLVNGRYLVTSKVHTPEELAELVKFLAAK
HCCCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA