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The map label for this gene is focA [H]

Identifier: 117920004

GI number: 117920004

Start: 1814601

End: 1815575

Strand: Direct

Name: focA [H]

Synonym: Shewana3_1556

Alternate gene names: 117920004

Gene position: 1814601-1815575 (Clockwise)

Preceding gene: 117920001

Following gene: 117920005

Centisome position: 36.49

GC content: 48.21

Gene sequence:

>975_bases
ATGAAAGACTCACATGCTAACGCTCCACAAACATCCTCGACTGGCCATGAGCCAATACAGGTTTCTTCACACTTTAGTTG
TTATCACCAAGCCGAAATTTACGGCAAAAGCAAAGTGGTTAAGGCGCCTTGGCAATCCTTTGGTTTAGCGGTATTTGCTG
GCGCTTTTATTGCCTTAGCCTTTGTGTTTTATCTGACAGTCACTACGGGTGCCGGTGATAGCGCCTGGGGTTTAGTGCGC
CTGGTGGGCGGGATTGCCTTCAGCTTAGGTTTAATTTTGGTGGTGATTTGCGGTGGCGAGCTGTTTACCAGTTCGGTGCT
GAGCAGCGTTGCATGGGCGCAAAAACAGGTGACCACCAGTGAATTGCTCAAATGCTGGAGCCGCGTGTATATCGGCAACT
TCGTCGGCGCCATGTTAATGGTTGGCTTGATCATGGCGGCGGGCTTGTATGAGCTCGATGGCGGTAACTGGGGCTTAAAC
GCGCTAAAAGTGTCGCAACATAAGCTGCATCATACTTGGGTGCAGGCATTCAGCTTAGGTATTTTATGTAACATGTTGGT
GTGTCTGGGTATTTGGATGACCTTCGCCAGCCGCGAATCCCTAACCAAAGCGATTTTACTGATCCTCCCTGTAGCCATGT
TTGTCAGCAGTGGCTTTGAGCACAGTATTGCAAACCTTTTTATGGTGCCGCTTGGGATCACTATTCAGTCGGTCGCCAGC
CCTGAGTTTTTTGCTTCTTTAGGCGTGACCCAAGAGCAATTTGCCGATTTAACCGTGGCAAACTTTGTATTTAATAACTT
AATTCCCGTTACCTTAGGGAACATTGTCGGTGGCGGCGTGATCGTTGGCCTTGGCTATTGGTGGATTGAGCAAGGTCAAA
TTGCCTTACCCGATCCGCAAAAATCCCATCAACCTCATTTTTTTGCATCGCCTCTGCATACTGAAAAGGCGAAAAATAGC
CAAGAAAGTAAGTAA

Upstream 100 bases:

>100_bases
TCTTAATGTAAATTTTGATTGGTTTGATCTGCGCCTGGGCGCTGTAGGAGAGACCTATGTTGGAAGTTCGCGTTTGTTTA
TCGGTTTTGGGAGTACAGTT

Downstream 100 bases:

>100_bases
GAGAGTCAGTTACTTAGGTTTGTGATAATTGTTCCTTGAGTGACTGTTTGCCTTAGGCTTATAAAAAATGCCCAGTGTTA
CTGGGCTTTTTTTGCTGTAT

Product: methionine gamma-lyase

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKDSHANAPQTSSTGHEPIQVSSHFSCYHQAEIYGKSKVVKAPWQSFGLAVFAGAFIALAFVFYLTVTTGAGDSAWGLVR
LVGGIAFSLGLILVVICGGELFTSSVLSSVAWAQKQVTTSELLKCWSRVYIGNFVGAMLMVGLIMAAGLYELDGGNWGLN
ALKVSQHKLHHTWVQAFSLGILCNMLVCLGIWMTFASRESLTKAILLILPVAMFVSSGFEHSIANLFMVPLGITIQSVAS
PEFFASLGVTQEQFADLTVANFVFNNLIPVTLGNIVGGGVIVGLGYWWIEQGQIALPDPQKSHQPHFFASPLHTEKAKNS
QESK

Sequences:

>Translated_324_residues
MKDSHANAPQTSSTGHEPIQVSSHFSCYHQAEIYGKSKVVKAPWQSFGLAVFAGAFIALAFVFYLTVTTGAGDSAWGLVR
LVGGIAFSLGLILVVICGGELFTSSVLSSVAWAQKQVTTSELLKCWSRVYIGNFVGAMLMVGLIMAAGLYELDGGNWGLN
ALKVSQHKLHHTWVQAFSLGILCNMLVCLGIWMTFASRESLTKAILLILPVAMFVSSGFEHSIANLFMVPLGITIQSVAS
PEFFASLGVTQEQFADLTVANFVFNNLIPVTLGNIVGGGVIVGLGYWWIEQGQIALPDPQKSHQPHFFASPLHTEKAKNS
QESK
>Mature_324_residues
MKDSHANAPQTSSTGHEPIQVSSHFSCYHQAEIYGKSKVVKAPWQSFGLAVFAGAFIALAFVFYLTVTTGAGDSAWGLVR
LVGGIAFSLGLILVVICGGELFTSSVLSSVAWAQKQVTTSELLKCWSRVYIGNFVGAMLMVGLIMAAGLYELDGGNWGLN
ALKVSQHKLHHTWVQAFSLGILCNMLVCLGIWMTFASRESLTKAILLILPVAMFVSSGFEHSIANLFMVPLGITIQSVAS
PEFFASLGVTQEQFADLTVANFVFNNLIPVTLGNIVGGGVIVGLGYWWIEQGQIALPDPQKSHQPHFFASPLHTEKAKNS
QESK

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=259, Percent_Identity=54.8262548262548, Blast_Score=291, Evalue=3e-80,
Organism=Escherichia coli, GI1788837, Length=263, Percent_Identity=39.9239543726236, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI87082249, Length=246, Percent_Identity=30.8943089430894, Blast_Score=76, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321779, Length=243, Percent_Identity=27.5720164609054, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 34913; Mature: 34913

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS01005 FORMATE_NITRITE_TP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSHANAPQTSSTGHEPIQVSSHFSCYHQAEIYGKSKVVKAPWQSFGLAVFAGAFIALA
CCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHH
FVFYLTVTTGAGDSAWGLVRLVGGIAFSLGLILVVICGGELFTSSVLSSVAWAQKQVTTS
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHH
ELLKCWSRVYIGNFVGAMLMVGLIMAAGLYELDGGNWGLNALKVSQHKLHHTWVQAFSLG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
ILCNMLVCLGIWMTFASRESLTKAILLILPVAMFVSSGFEHSIANLFMVPLGITIQSVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCC
PEFFASLGVTQEQFADLTVANFVFNNLIPVTLGNIVGGGVIVGLGYWWIEQGQIALPDPQ
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHEECHHHEEECCCEECCCCC
KSHQPHFFASPLHTEKAKNSQESK
CCCCCCEECCCCHHHHHCCCCCCC
>Mature Secondary Structure
MKDSHANAPQTSSTGHEPIQVSSHFSCYHQAEIYGKSKVVKAPWQSFGLAVFAGAFIALA
CCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHH
FVFYLTVTTGAGDSAWGLVRLVGGIAFSLGLILVVICGGELFTSSVLSSVAWAQKQVTTS
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHH
ELLKCWSRVYIGNFVGAMLMVGLIMAAGLYELDGGNWGLNALKVSQHKLHHTWVQAFSLG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
ILCNMLVCLGIWMTFASRESLTKAILLILPVAMFVSSGFEHSIANLFMVPLGITIQSVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCC
PEFFASLGVTQEQFADLTVANFVFNNLIPVTLGNIVGGGVIVGLGYWWIEQGQIALPDPQ
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHEECHHHEEECCCEECCCCC
KSHQPHFFASPLHTEKAKNSQESK
CCCCCCEECCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]