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The map label for this gene is rluB [H]

Identifier: 117919973

GI number: 117919973

Start: 1775428

End: 1776303

Strand: Direct

Name: rluB [H]

Synonym: Shewana3_1525

Alternate gene names: 117919973

Gene position: 1775428-1776303 (Clockwise)

Preceding gene: 117919972

Following gene: 117919974

Centisome position: 35.71

GC content: 53.08

Gene sequence:

>876_bases
ATGAGTGAAAAATTGCAGAAAGTCTTGGCCCGTGCAGGCCATGGCTCCCGTCGTGAGATGGAGGCATGGATTGCTGCAGG
CCGAGTTAGTATTGATGGCGTGATTGCCAATCTTGGCGACCGTATCGAAGCCGATGCGAAGGTTCGTATCGATGGTAGAG
CCGTATCGCTTAAGTCCGCTGACGATGTGATTTGCCGCGTACTCGCCTATCACAAACCCGAAGGTGAGATTTGTAGCCGT
AAAGACCCTGAAGGTCGTCCAACCGTGTTTGACCGTTTGCCGAAAGTGCGTGACTCCCGTTGGGTTGCGGTCGGCCGTCT
GGATATCAACACTTCAGGCTTATTGCTGTTTACCTCAGATGGTGAATTAGCAAACCGTTTGATGCACCCTTCTAACGAAG
TTGAACGTGAATACGCAGTACGTACCTTTGGTGAGGTGCCTGAGGCTGCGGTACAACGTCTGCGTACCGGCGTAATGTTA
GAAGACGGCATGGCGCAGTTCGATGCCATCAAAGCCGCAGGCGGCGAGGGCATGAACCAATGGTGGCACGTATGTTTACG
TGAAGGCCGCAACCGCGAAGTGCGTCGTTTATGGGAATCCCAAGAGGTGCAAGTTAGCCGTCTGATCCGTGTGCGTTACG
GTATGGTGGAATTGCCTAAATCTCTGCCTCGTGGTGGCTGGGTTGAATTGCCTATTGAGCAAGTTAACTACCTGCGCCAA
TTAGCGGGTCTTGAACCTGAAACTCGCACTATGCTGGCCGCCGATAAACACAGCGTGGCCCGTGCTCAGGTAAAGAGTGC
TAAAATTCGCCGCGCCGTACGCAAGCATAAAGTGACTGCGACGCCTAAGGCTAAGCCAACACGTCAGCGTAGTTAA

Upstream 100 bases:

>100_bases
ACCCTAGCGGATTTACCTGCGCTTACGGATACTGAGTCATTGCAGGCGGTATTTTCAGGTTTGAAAGTAACGCCTGAACA
GTCAGAAGAGCAAGATACTA

Downstream 100 bases:

>100_bases
TATCTTTCGAGTGATGTTTAGTCTCTCAGTAAAAAGGCCGCATGCGGCCTTTTTTGCTTTCTGCGGCCAGCGGTTATGCT
ATAACAAACCTAAAGATGAC

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSEKLQKVLARAGHGSRREMEAWIAAGRVSIDGVIANLGDRIEADAKVRIDGRAVSLKSADDVICRVLAYHKPEGEICSR
KDPEGRPTVFDRLPKVRDSRWVAVGRLDINTSGLLLFTSDGELANRLMHPSNEVEREYAVRTFGEVPEAAVQRLRTGVML
EDGMAQFDAIKAAGGEGMNQWWHVCLREGRNREVRRLWESQEVQVSRLIRVRYGMVELPKSLPRGGWVELPIEQVNYLRQ
LAGLEPETRTMLAADKHSVARAQVKSAKIRRAVRKHKVTATPKAKPTRQRS

Sequences:

>Translated_291_residues
MSEKLQKVLARAGHGSRREMEAWIAAGRVSIDGVIANLGDRIEADAKVRIDGRAVSLKSADDVICRVLAYHKPEGEICSR
KDPEGRPTVFDRLPKVRDSRWVAVGRLDINTSGLLLFTSDGELANRLMHPSNEVEREYAVRTFGEVPEAAVQRLRTGVML
EDGMAQFDAIKAAGGEGMNQWWHVCLREGRNREVRRLWESQEVQVSRLIRVRYGMVELPKSLPRGGWVELPIEQVNYLRQ
LAGLEPETRTMLAADKHSVARAQVKSAKIRRAVRKHKVTATPKAKPTRQRS
>Mature_290_residues
SEKLQKVLARAGHGSRREMEAWIAAGRVSIDGVIANLGDRIEADAKVRIDGRAVSLKSADDVICRVLAYHKPEGEICSRK
DPEGRPTVFDRLPKVRDSRWVAVGRLDINTSGLLLFTSDGELANRLMHPSNEVEREYAVRTFGEVPEAAVQRLRTGVMLE
DGMAQFDAIKAAGGEGMNQWWHVCLREGRNREVRRLWESQEVQVSRLIRVRYGMVELPKSLPRGGWVELPIEQVNYLRQL
AGLEPETRTMLAADKHSVARAQVKSAKIRRAVRKHKVTATPKAKPTRQRS

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=278, Percent_Identity=66.9064748201439, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1790453, Length=285, Percent_Identity=27.0175438596491, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=28.6324786324786, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081838, Length=202, Percent_Identity=28.7128712871287, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 32758; Mature: 32627

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLQKVLARAGHGSRREMEAWIAAGRVSIDGVIANLGDRIEADAKVRIDGRAVSLKSA
CCHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCEEEECCEEEEECCH
DDVICRVLAYHKPEGEICSRKDPEGRPTVFDRLPKVRDSRWVAVGRLDINTSGLLLFTSD
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEEEECCCEEEEEECC
GELANRLMHPSNEVEREYAVRTFGEVPEAAVQRLRTGVMLEDGMAQFDAIKAAGGEGMNQ
CHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCHHHHHHHHHCCCCCHHH
WWHVCLREGRNREVRRLWESQEVQVSRLIRVRYGMVELPKSLPRGGWVELPIEQVNYLRQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHH
LAGLEPETRTMLAADKHSVARAQVKSAKIRRAVRKHKVTATPKAKPTRQRS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEKLQKVLARAGHGSRREMEAWIAAGRVSIDGVIANLGDRIEADAKVRIDGRAVSLKSA
CHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCEEEECCEEEEECCH
DDVICRVLAYHKPEGEICSRKDPEGRPTVFDRLPKVRDSRWVAVGRLDINTSGLLLFTSD
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEEEECCCEEEEEECC
GELANRLMHPSNEVEREYAVRTFGEVPEAAVQRLRTGVMLEDGMAQFDAIKAAGGEGMNQ
CHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCHHHHHHHHHCCCCCHHH
WWHVCLREGRNREVRRLWESQEVQVSRLIRVRYGMVELPKSLPRGGWVELPIEQVNYLRQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHH
LAGLEPETRTMLAADKHSVARAQVKSAKIRRAVRKHKVTATPKAKPTRQRS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]