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The map label for this gene is folC [H]

Identifier: 117919928

GI number: 117919928

Start: 1723007

End: 1724305

Strand: Direct

Name: folC [H]

Synonym: Shewana3_1480

Alternate gene names: 117919928

Gene position: 1723007-1724305 (Clockwise)

Preceding gene: 117919927

Following gene: 117919929

Centisome position: 34.65

GC content: 52.66

Gene sequence:

>1299_bases
ATGACCACAGCCCCGACAGAGCAGACATCTTTAACTGTGCCGAGTGCCAATGCGCCACTGACACAATGGTTAGATTATTT
ATTATCGATTCACCCAACCGAAATCGATATGGGATTAACCCGGGTTTCAAAGGTTGCCGCGCGCCTGCAATTATTGGATC
TCGGGCCTAGCAAAGTGGTCACTGTAGGGGGCACCAATGGTAAAGGCACCACCTGCGCCATGCTCGAGAATATCCTGCGT
TTATCTGGTCTGACGGTGGGCGTCTACAGCTCGCCCCATATGCTCAAGTACAACGAAAGGGTGCGCATTAACGGTCAGGA
CGCCAGCGATGAAGCCTTTGTTGCTGCCTTTGAACAAATTGAGGCAGCGCGCGGCGAGATTTCGCTGACCTTCTTCGAAT
ATGCCACCTTGGCGGGCTTAATTCTGTTTAAAGCGGCCAAGTTGGATGTGGTGATCTTAGAAGTCGGTCTTGGCGGCCGT
TTGGATGCCACCAACATGATTGATGCCGATATCAGCGTGGTGACGTCCATCGATTTAGACCACGAGGCCTATTTGGGCAA
TACCCGCGAGCTGGTCGGCCGTGAAAAGGCGGGCATTTTTCGCCAAGGTCGCCCTGCGATTGTCGGCGAGCCAAACTTAC
CCCATACGGTCAATGATGTCGCCCAAGATGTTGGCGCCAAGCTCTATCGTGTGGGCGAGGAGTTTAGTTATCAGCAAGAT
GGCCAGACGTGGGATTACCAAGGCCAAGTATTCAACCTTAAGCAGCTGAGTTTGCCGACACTGCCATTGCCGAACGCCGC
GACTGTGATTGCGATTGTTGAGCATGGCTGGCCGCATATTACCGCCGATATCATTGACAAAGGCATCAGCAGTGCGCGCT
TAACCGGGCGTTTAGAGCAAGTTAACGAGCAGCCGCTGATTTTACTCGATGTGGCCCACAATCCCCATGCGGCGCGCTTT
TTAGTCAAGCAGCTCACGCCAATGGTGACGGGGAAGCGAGTGTTTGCCCTGTGCGGCATGCTAAAGGATAAGGACATTGC
TGGCGTATTGCCTGTGATTTCGCCCTTAATCGATACTTGGTACTTAGTGAGTTTACAGGGGGAGCGCGGCGCGAATGCAA
GCCTGCTGCGCCAAACCTTAGCGCAAGACACTAAGGCCCATGAGTTTGGCGATATCGCCGCGGCATGGACGGCATTAAAA
GCGCAAATTCAGCCCGATGATGTGGTAATTGTGTTTGGCTCCTTTTACACTGTGGCAGGTTTTAAGTCTCTCTTTAAGCA
GGATAACCGTTTTGTCTAG

Upstream 100 bases:

>100_bases
GTATCAACTGCCTAAGTTGGCTTTAGGGCCATTGTTTATGCTGGATTAATCGCCGCTTTGCTCGTTAAGTGAAGCGCGTA
TCGAAGGAAGGAAAGAACAG

Downstream 100 bases:

>100_bases
CCAGTTTCATAATCGATTAGTCGGCACTGTGGTGCTGGTCGCCCTCGGGGTGATTTTTCTGCCGGATATTTTAGATGGCA
AGAAGGAGCGTCAAGAGGAA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTTAPTEQTSLTVPSANAPLTQWLDYLLSIHPTEIDMGLTRVSKVAARLQLLDLGPSKVVTVGGTNGKGTTCAMLENILR
LSGLTVGVYSSPHMLKYNERVRINGQDASDEAFVAAFEQIEAARGEISLTFFEYATLAGLILFKAAKLDVVILEVGLGGR
LDATNMIDADISVVTSIDLDHEAYLGNTRELVGREKAGIFRQGRPAIVGEPNLPHTVNDVAQDVGAKLYRVGEEFSYQQD
GQTWDYQGQVFNLKQLSLPTLPLPNAATVIAIVEHGWPHITADIIDKGISSARLTGRLEQVNEQPLILLDVAHNPHAARF
LVKQLTPMVTGKRVFALCGMLKDKDIAGVLPVISPLIDTWYLVSLQGERGANASLLRQTLAQDTKAHEFGDIAAAWTALK
AQIQPDDVVIVFGSFYTVAGFKSLFKQDNRFV

Sequences:

>Translated_432_residues
MTTAPTEQTSLTVPSANAPLTQWLDYLLSIHPTEIDMGLTRVSKVAARLQLLDLGPSKVVTVGGTNGKGTTCAMLENILR
LSGLTVGVYSSPHMLKYNERVRINGQDASDEAFVAAFEQIEAARGEISLTFFEYATLAGLILFKAAKLDVVILEVGLGGR
LDATNMIDADISVVTSIDLDHEAYLGNTRELVGREKAGIFRQGRPAIVGEPNLPHTVNDVAQDVGAKLYRVGEEFSYQQD
GQTWDYQGQVFNLKQLSLPTLPLPNAATVIAIVEHGWPHITADIIDKGISSARLTGRLEQVNEQPLILLDVAHNPHAARF
LVKQLTPMVTGKRVFALCGMLKDKDIAGVLPVISPLIDTWYLVSLQGERGANASLLRQTLAQDTKAHEFGDIAAAWTALK
AQIQPDDVVIVFGSFYTVAGFKSLFKQDNRFV
>Mature_431_residues
TTAPTEQTSLTVPSANAPLTQWLDYLLSIHPTEIDMGLTRVSKVAARLQLLDLGPSKVVTVGGTNGKGTTCAMLENILRL
SGLTVGVYSSPHMLKYNERVRINGQDASDEAFVAAFEQIEAARGEISLTFFEYATLAGLILFKAAKLDVVILEVGLGGRL
DATNMIDADISVVTSIDLDHEAYLGNTRELVGREKAGIFRQGRPAIVGEPNLPHTVNDVAQDVGAKLYRVGEEFSYQQDG
QTWDYQGQVFNLKQLSLPTLPLPNAATVIAIVEHGWPHITADIIDKGISSARLTGRLEQVNEQPLILLDVAHNPHAARFL
VKQLTPMVTGKRVFALCGMLKDKDIAGVLPVISPLIDTWYLVSLQGERGANASLLRQTLAQDTKAHEFGDIAAAWTALKA
QIQPDDVVIVFGSFYTVAGFKSLFKQDNRFV

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=191, Percent_Identity=39.7905759162304, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI66932984, Length=191, Percent_Identity=39.7905759162304, Blast_Score=133, Evalue=4e-31,
Organism=Escherichia coli, GI1788654, Length=406, Percent_Identity=51.9704433497537, Blast_Score=402, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71984923, Length=160, Percent_Identity=37.5, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17553150, Length=160, Percent_Identity=37.5, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17553148, Length=160, Percent_Identity=37.5, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323760, Length=349, Percent_Identity=27.7936962750716, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324815, Length=181, Percent_Identity=32.0441988950276, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24641571, Length=181, Percent_Identity=35.9116022099448, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24581568, Length=187, Percent_Identity=33.1550802139037, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 46989; Mature: 46858

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00178 AA_TRNA_LIGASE_I ; PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAPTEQTSLTVPSANAPLTQWLDYLLSIHPTEIDMGLTRVSKVAARLQLLDLGPSKVV
CCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCCCEE
TVGGTNGKGTTCAMLENILRLSGLTVGVYSSPHMLKYNERVRINGQDASDEAFVAAFEQI
EECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEECCCCCCCHHHHHHHHHH
EAARGEISLTFFEYATLAGLILFKAAKLDVVILEVGLGGRLDATNMIDADISVVTSIDLD
HHCCCCEEEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCEEEEEEECCC
HEAYLGNTRELVGREKAGIFRQGRPAIVGEPNLPHTVNDVAQDVGAKLYRVGEEFSYQQD
CHHHCCCHHHHHCCHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCHHCCCCCC
GQTWDYQGQVFNLKQLSLPTLPLPNAATVIAIVEHGWPHITADIIDKGISSARLTGRLEQ
CCCCCCCCCEEEEHCCCCCCCCCCCCCEEEEEEECCCCEEEHHHHHCCCCHHHHHHHHHH
VNEQPLILLDVAHNPHAARFLVKQLTPMVTGKRVFALCGMLKDKDIAGVLPVISPLIDTW
CCCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCE
YLVSLQGERGANASLLRQTLAQDTKAHEFGDIAAAWTALKAQIQPDDVVIVFGSFYTVAG
EEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHH
FKSLFKQDNRFV
HHHHHHCCCCCC
>Mature Secondary Structure 
TTAPTEQTSLTVPSANAPLTQWLDYLLSIHPTEIDMGLTRVSKVAARLQLLDLGPSKVV
CCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCCCEE
TVGGTNGKGTTCAMLENILRLSGLTVGVYSSPHMLKYNERVRINGQDASDEAFVAAFEQI
EECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEECCCCCCCHHHHHHHHHH
EAARGEISLTFFEYATLAGLILFKAAKLDVVILEVGLGGRLDATNMIDADISVVTSIDLD
HHCCCCEEEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCEEEEEEECCC
HEAYLGNTRELVGREKAGIFRQGRPAIVGEPNLPHTVNDVAQDVGAKLYRVGEEFSYQQD
CHHHCCCHHHHHCCHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCHHCCCCCC
GQTWDYQGQVFNLKQLSLPTLPLPNAATVIAIVEHGWPHITADIIDKGISSARLTGRLEQ
CCCCCCCCCEEEEHCCCCCCCCCCCCCEEEEEEECCCCEEEHHHHHCCCCHHHHHHHHHH
VNEQPLILLDVAHNPHAARFLVKQLTPMVTGKRVFALCGMLKDKDIAGVLPVISPLIDTW
CCCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCE
YLVSLQGERGANASLLRQTLAQDTKAHEFGDIAAAWTALKAQIQPDDVVIVFGSFYTVAG
EEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHH
FKSLFKQDNRFV
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]