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The map label for this gene is pdxB [H]

Identifier: 117919924

GI number: 117919924

Start: 1716390

End: 1717520

Strand: Direct

Name: pdxB [H]

Synonym: Shewana3_1476

Alternate gene names: 117919924

Gene position: 1716390-1717520 (Clockwise)

Preceding gene: 117919923

Following gene: 117919925

Centisome position: 34.52

GC content: 50.4

Gene sequence:

>1131_bases
ATGAAGATTGTTGTCGATGAAAATATGCCCTATGTCGAACCCTTATTTGGGGCGTTAGGCGAGATAATCCCAGTCAATGG
TCGCACGCTGACGCCTGAGCAGGTGCAAGATGCCGATGTACTGCTGGTGCGCTCTGTCACCCGAGTCAATGCTGCATTGT
TAGATGCCAATTCGAAACTTAAGTTTGTCGGCAGTGCCACCATTGGGACGGACCATGTGGATCTGGCTTACCTTGCCGGG
CGTGGCATCCCCTTTTCGAATGCGCCCGGCTGTAATGCGACCGCTGTCGGGGAGTTTGCCTTTATTGCGATGTTAGAACT
GGCGGCGCGGTTTAATTCGCCGCTCAAGGGCAAGGTCGTGGGAATTGTCGGTGCAGGCAATACTGGTAGCGCGACGGCCA
AATGCCTCGAAGCCTACGGCATTAAGGTATTACTCAATGACCCCATCAAAGCGGCCGAAGGCGATCCCCGTCATTTTGTG
AGCCTTGAGACCCTATTACATGAAGCGGATATCATCAGCTTACATGTGCCCATCACTCGCACTGGCGAGCATAAAACGCT
GCATTTATTCGATGAAGCGCGCATGATGAGTCTTAAACCCAATACCTGGCTGCTGAACTGTTGTCGTGGTGATGTGATTG
ATAACCAAGCCTTAATTAAGGTCAAAGAGCAAAGGGATGACCTTAAATTGGTGCTCGATGTATGGGAAGGGGAGCCAAAT
CCCATGCCGGAGCTGGTGCCTTTTGCGGAGTTTGCGACCCCGCATATCGCCGGTTACAGCTTAGAAGGTAAGGCACGTGG
CACTTTTATGCTGTACCAAAAGTTGTGTGAGTTGCTAGCCATTCCGGCCACTAAACGCTTATCCGAGCTATTGCCTCCAT
TTCATTTTAAGGCGGTTGAACTCGAACAAGCCCCCGATGAAAAAGCCTTATTACAGCTGGCGAGATTCGTCTACGACCTG
CGTGATGACGATGCCGTATTCCGAAATGGCTTTGCAAGGTCGAATGGTTTCGACACTATGAGAAAAAATCACAAACATAG
GCGCGAATTTAGTGCATTGGCGCTGGCTTATCACGGCCAATCTGAGGTAGATTGGTTGTCCAATTTAGGTTTTTCAGGAG
TCGGTCGGTAA

Upstream 100 bases:

>100_bases
CGATTGGTATAATGCGACTCAAGGATTGGGTTTATGATCAGAGTCTTGCTAGAATCCTTGGCTACTCACTCTCTAGCCCA
TTTTTATCAGAAAGGTCCCC

Downstream 100 bases:

>100_bases
TTATGTCGCAGGAATTTAATGTTGTCGTTTTAGGTGCATCGGGTGCAGTGGGTCAAACAATGATTGAGATCCTCGAAGAG
CGTAACTTCCCGGTTGCGAA

Product: D-isomer specific 2-hydroxyacid dehydrogenase, catalytic region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKIVVDENMPYVEPLFGALGEIIPVNGRTLTPEQVQDADVLLVRSVTRVNAALLDANSKLKFVGSATIGTDHVDLAYLAG
RGIPFSNAPGCNATAVGEFAFIAMLELAARFNSPLKGKVVGIVGAGNTGSATAKCLEAYGIKVLLNDPIKAAEGDPRHFV
SLETLLHEADIISLHVPITRTGEHKTLHLFDEARMMSLKPNTWLLNCCRGDVIDNQALIKVKEQRDDLKLVLDVWEGEPN
PMPELVPFAEFATPHIAGYSLEGKARGTFMLYQKLCELLAIPATKRLSELLPPFHFKAVELEQAPDEKALLQLARFVYDL
RDDDAVFRNGFARSNGFDTMRKNHKHRREFSALALAYHGQSEVDWLSNLGFSGVGR

Sequences:

>Translated_376_residues
MKIVVDENMPYVEPLFGALGEIIPVNGRTLTPEQVQDADVLLVRSVTRVNAALLDANSKLKFVGSATIGTDHVDLAYLAG
RGIPFSNAPGCNATAVGEFAFIAMLELAARFNSPLKGKVVGIVGAGNTGSATAKCLEAYGIKVLLNDPIKAAEGDPRHFV
SLETLLHEADIISLHVPITRTGEHKTLHLFDEARMMSLKPNTWLLNCCRGDVIDNQALIKVKEQRDDLKLVLDVWEGEPN
PMPELVPFAEFATPHIAGYSLEGKARGTFMLYQKLCELLAIPATKRLSELLPPFHFKAVELEQAPDEKALLQLARFVYDL
RDDDAVFRNGFARSNGFDTMRKNHKHRREFSALALAYHGQSEVDWLSNLGFSGVGR
>Mature_376_residues
MKIVVDENMPYVEPLFGALGEIIPVNGRTLTPEQVQDADVLLVRSVTRVNAALLDANSKLKFVGSATIGTDHVDLAYLAG
RGIPFSNAPGCNATAVGEFAFIAMLELAARFNSPLKGKVVGIVGAGNTGSATAKCLEAYGIKVLLNDPIKAAEGDPRHFV
SLETLLHEADIISLHVPITRTGEHKTLHLFDEARMMSLKPNTWLLNCCRGDVIDNQALIKVKEQRDDLKLVLDVWEGEPN
PMPELVPFAEFATPHIAGYSLEGKARGTFMLYQKLCELLAIPATKRLSELLPPFHFKAVELEQAPDEKALLQLARFVYDL
RDDDAVFRNGFARSNGFDTMRKNHKHRREFSALALAYHGQSEVDWLSNLGFSGVGR

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=255, Percent_Identity=27.843137254902, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI145580578, Length=159, Percent_Identity=30.8176100628931, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4557499, Length=159, Percent_Identity=30.8176100628931, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI145580575, Length=159, Percent_Identity=30.8176100628931, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788660, Length=374, Percent_Identity=47.3262032085562, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI1789279, Length=257, Percent_Identity=29.9610894941634, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1787645, Length=199, Percent_Identity=28.643216080402, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082289, Length=268, Percent_Identity=27.6119402985075, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=252, Percent_Identity=28.1746031746032, Blast_Score=98, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6320925, Length=266, Percent_Identity=28.1954887218045, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322116, Length=266, Percent_Identity=28.1954887218045, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324055, Length=225, Percent_Identity=24.8888888888889, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921140, Length=265, Percent_Identity=26.0377358490566, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24646446, Length=245, Percent_Identity=27.3469387755102, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24646448, Length=245, Percent_Identity=27.3469387755102, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24646452, Length=245, Percent_Identity=27.3469387755102, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24646450, Length=245, Percent_Identity=27.3469387755102, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI62472511, Length=245, Percent_Identity=27.3469387755102, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28574286, Length=266, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI28574284, Length=272, Percent_Identity=25, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585514, Length=272, Percent_Identity=25, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28574282, Length=272, Percent_Identity=25, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45552429, Length=272, Percent_Identity=25, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45551003, Length=272, Percent_Identity=25, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.290 [H]

Molecular weight: Translated: 41330; Mature: 41330

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVVDENMPYVEPLFGALGEIIPVNGRTLTPEQVQDADVLLVRSVTRVNAALLDANSKL
CEEEEECCCCHHHHHHHHHCCEEECCCCEECHHHHCCCCEEEHHHHHHHHHHEECCCCCE
KFVGSATIGTDHVDLAYLAGRGIPFSNAPGCNATAVGEFAFIAMLELAARFNSPLKGKVV
EEEEECCCCCCCCEEHHECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
GIVGAGNTGSATAKCLEAYGIKVLLNDPIKAAEGDPRHFVSLETLLHEADIISLHVPITR
EEEECCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECEEE
TGEHKTLHLFDEARMMSLKPNTWLLNCCRGDVIDNQALIKVKEQRDDLKLVLDVWEGEPN
CCCCCEEEEECCCEEEEECCCCEEEEECCCCCCCCCEEEEEECCCCCEEEEEEEECCCCC
PMPELVPFAEFATPHIAGYSLEGKARGTFMLYQKLCELLAIPATKRLSELLPPFHFKAVE
CCCCCCCHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEE
LEQAPDEKALLQLARFVYDLRDDDAVFRNGFARSNGFDTMRKNHKHRREFSALALAYHGQ
CCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCC
SEVDWLSNLGFSGVGR
CHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKIVVDENMPYVEPLFGALGEIIPVNGRTLTPEQVQDADVLLVRSVTRVNAALLDANSKL
CEEEEECCCCHHHHHHHHHCCEEECCCCEECHHHHCCCCEEEHHHHHHHHHHEECCCCCE
KFVGSATIGTDHVDLAYLAGRGIPFSNAPGCNATAVGEFAFIAMLELAARFNSPLKGKVV
EEEEECCCCCCCCEEHHECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
GIVGAGNTGSATAKCLEAYGIKVLLNDPIKAAEGDPRHFVSLETLLHEADIISLHVPITR
EEEECCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECEEE
TGEHKTLHLFDEARMMSLKPNTWLLNCCRGDVIDNQALIKVKEQRDDLKLVLDVWEGEPN
CCCCCEEEEECCCEEEEECCCCEEEEECCCCCCCCCEEEEEECCCCCEEEEEEEECCCCC
PMPELVPFAEFATPHIAGYSLEGKARGTFMLYQKLCELLAIPATKRLSELLPPFHFKAVE
CCCCCCCHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEE
LEQAPDEKALLQLARFVYDLRDDDAVFRNGFARSNGFDTMRKNHKHRREFSALALAYHGQ
CCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCC
SEVDWLSNLGFSGVGR
CHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]