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The map label for this gene is rfbB [H]

Identifier: 117919827

GI number: 117919827

Start: 1594422

End: 1595501

Strand: Direct

Name: rfbB [H]

Synonym: Shewana3_1379

Alternate gene names: 117919827

Gene position: 1594422-1595501 (Clockwise)

Preceding gene: 117919826

Following gene: 117919828

Centisome position: 32.07

GC content: 41.76

Gene sequence:

>1080_bases
ATGAAAATTTTAGTAACAGGTGGTGCTGGTTTTATTGGTTCGGCTGTGATTCGACACATAATAATGAACACCAATGACAG
CGTAATTAACGTAGATAAACTGACGTACGCTGGAAATTTAGAATCCTTAAAATTGGTTAGCACTAATCCACGGTATAACT
TCGAGCAAGTCGATATTTGTGATCGTGCAACACTCGAGAGAGTTTTTTCACAATACCAACCCGATGCTGTTATGCATCTA
GCTGCAGAATCTCATGTAGATCGCTCAATCACTGGCCCTAGCGATTTTATCCAAACTAATATTGTGGGGACTTACATATT
ACTCGAAGCCGCTCGTCAATATTGGACACAACTCGATGCTGAACGTAAAGCTGCATTCCGTTTTCACCATATTTCAACTG
ATGAAGTCTATGGTGATTTGCCACATCCGGATGAGCAAGAAGGACAAGCAGTAAATCAATACCTGCCTTTATTTACCGAG
ACGACGCCTTATGCTCCGAGCAGCCCATATTCAGCCTCTAAAGCTTCAAGTGACCATCTGGTACGAGCTTGGCTTCGTAC
CTACGGATTTCCAACCATAGTGACAAACTGCTCAAATAACTATGGTCCTTATCATTTTCCTGAAAAGTTGATCCCTCTGG
TAATTCTCAATGCACTTGAAGGTAAGTCATTACCTATTTACGGTAAAGGTGATCAAATCCGTGATTGGTTATATGTTGAA
GACCATGCTCGTGCGTTATACAAAGTCGTTACCGAAGGTAAAGTTGGTGAAACCTACAATATTGGTGGTCATAATGAGAA
GCAGAACATAGAGGTGGTGAAAACCATTTGTAGTATCTTAGATTCATTAGTGCCCAAAGCTACGCCTTATGCCGAGCAAA
TTACTTTTGTGACCGATAGGCCTGGCCATGATCGCCGTTATGCCATTGATGCAAGTAAAATGAGTGCAGAACTAAATTGG
CAGCCCCAAGAAACTTTCGAAACTGGCTTACGTAAAACCATCGAATGGTATTTAGCTAACCAAGAATGGTGCCAGCATGT
ACAAGACGGCACCTATCAGCGTGAGCGTTTAGGTATTTAA

Upstream 100 bases:

>100_bases
TCGAGATGTACTTGGTTTGAACTTTTTTAAAACGATTAAATGTTTTATTTTATATGCCTATAAAGGCTTCTTGAAAAGTA
GAATATGAAAGAGTAATTAA

Downstream 100 bases:

>100_bases
TCACTTTAACAACTTTATTTTAAAGTCCTAGTTTTCACCTAGGGCCTATTTACCTAGGAACTTGATTTAAATGAAAGGAA
TTATTTTAGCTGGCGGTTCT

Product: dTDP-glucose 4,6 dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MKILVTGGAGFIGSAVIRHIIMNTNDSVINVDKLTYAGNLESLKLVSTNPRYNFEQVDICDRATLERVFSQYQPDAVMHL
AAESHVDRSITGPSDFIQTNIVGTYILLEAARQYWTQLDAERKAAFRFHHISTDEVYGDLPHPDEQEGQAVNQYLPLFTE
TTPYAPSSPYSASKASSDHLVRAWLRTYGFPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKSLPIYGKGDQIRDWLYVE
DHARALYKVVTEGKVGETYNIGGHNEKQNIEVVKTICSILDSLVPKATPYAEQITFVTDRPGHDRRYAIDASKMSAELNW
QPQETFETGLRKTIEWYLANQEWCQHVQDGTYQRERLGI

Sequences:

>Translated_359_residues
MKILVTGGAGFIGSAVIRHIIMNTNDSVINVDKLTYAGNLESLKLVSTNPRYNFEQVDICDRATLERVFSQYQPDAVMHL
AAESHVDRSITGPSDFIQTNIVGTYILLEAARQYWTQLDAERKAAFRFHHISTDEVYGDLPHPDEQEGQAVNQYLPLFTE
TTPYAPSSPYSASKASSDHLVRAWLRTYGFPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKSLPIYGKGDQIRDWLYVE
DHARALYKVVTEGKVGETYNIGGHNEKQNIEVVKTICSILDSLVPKATPYAEQITFVTDRPGHDRRYAIDASKMSAELNW
QPQETFETGLRKTIEWYLANQEWCQHVQDGTYQRERLGI
>Mature_359_residues
MKILVTGGAGFIGSAVIRHIIMNTNDSVINVDKLTYAGNLESLKLVSTNPRYNFEQVDICDRATLERVFSQYQPDAVMHL
AAESHVDRSITGPSDFIQTNIVGTYILLEAARQYWTQLDAERKAAFRFHHISTDEVYGDLPHPDEQEGQAVNQYLPLFTE
TTPYAPSSPYSASKASSDHLVRAWLRTYGFPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKSLPIYGKGDQIRDWLYVE
DHARALYKVVTEGKVGETYNIGGHNEKQNIEVVKTICSILDSLVPKATPYAEQITFVTDRPGHDRRYAIDASKMSAELNW
QPQETFETGLRKTIEWYLANQEWCQHVQDGTYQRERLGI

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=340, Percent_Identity=34.4117647058824, Blast_Score=215, Evalue=7e-56,
Organism=Homo sapiens, GI42516563, Length=345, Percent_Identity=23.768115942029, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI56237023, Length=363, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI56118217, Length=363, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI189083684, Length=363, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI1788353, Length=353, Percent_Identity=77.3371104815864, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI48994969, Length=362, Percent_Identity=70.7182320441989, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1786974, Length=364, Percent_Identity=27.1978021978022, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788366, Length=370, Percent_Identity=24.5945945945946, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17568069, Length=345, Percent_Identity=37.3913043478261, Blast_Score=211, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI115532424, Length=338, Percent_Identity=31.3609467455621, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71982035, Length=381, Percent_Identity=28.0839895013123, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71982038, Length=383, Percent_Identity=27.9373368146214, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17539532, Length=343, Percent_Identity=25.0728862973761, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319493, Length=373, Percent_Identity=27.8820375335121, Blast_Score=96, Evalue=9e-21,
Organism=Drosophila melanogaster, GI19923002, Length=368, Percent_Identity=26.3586956521739, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21356223, Length=341, Percent_Identity=24.9266862170088, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40667; Mature: 40667

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAVIRHIIMNTNDSVINVDKLTYAGNLESLKLVSTNPRYNFEQVDIC
CEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEECCCCCCCCCCCHH
DRATLERVFSQYQPDAVMHLAAESHVDRSITGPSDFIQTNIVGTYILLEAARQYWTQLDA
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCH
ERKAAFRFHHISTDEVYGDLPHPDEQEGQAVNQYLPLFTETTPYAPSSPYSASKASSDHL
HHHHHEEEEECCHHHHCCCCCCCCCHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCHHH
VRAWLRTYGFPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKSLPIYGKGDQIRDWLYVE
HHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEH
DHARALYKVVTEGKVGETYNIGGHNEKQNIEVVKTICSILDSLVPKATPYAEQITFVTDR
HHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEECC
PGHDRRYAIDASKMSAELNWQPQETFETGLRKTIEWYLANQEWCQHVQDGTYQRERLGI
CCCCCEEEEEHHHHCEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MKILVTGGAGFIGSAVIRHIIMNTNDSVINVDKLTYAGNLESLKLVSTNPRYNFEQVDIC
CEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEECCCCCCCCCCCHH
DRATLERVFSQYQPDAVMHLAAESHVDRSITGPSDFIQTNIVGTYILLEAARQYWTQLDA
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCH
ERKAAFRFHHISTDEVYGDLPHPDEQEGQAVNQYLPLFTETTPYAPSSPYSASKASSDHL
HHHHHEEEEECCHHHHCCCCCCCCCHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCHHH
VRAWLRTYGFPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKSLPIYGKGDQIRDWLYVE
HHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEH
DHARALYKVVTEGKVGETYNIGGHNEKQNIEVVKTICSILDSLVPKATPYAEQITFVTDR
HHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEECC
PGHDRRYAIDASKMSAELNWQPQETFETGLRKTIEWYLANQEWCQHVQDGTYQRERLGI
CCCCCEEEEEHHHHCEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]