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The map label for this gene is fliA [H]

Identifier: 117919807

GI number: 117919807

Start: 1572224

End: 1572937

Strand: Direct

Name: fliA [H]

Synonym: Shewana3_1359

Alternate gene names: 117919807

Gene position: 1572224-1572937 (Clockwise)

Preceding gene: 117919806

Following gene: 117919808

Centisome position: 31.62

GC content: 44.54

Gene sequence:

>714_bases
GTGAATAAAGCCGCAGCGTATACTTGTTTAGACAGTAAAACGTCAATCGTTGAACAGTATGCACCGTTGGTTAAAAGAAT
TGCACATCACTTGCTCGCCAGATTACCTGCCTCAGTGCAGCTCGATGACTTGCTTCAAGCGGGGATGATGGGGTTATTAG
AAGCATCATCCAAGTTTGATGGTGGCAAAGGTGCAAAGTTTGAAACCTTCGCCGGCATACGGATCCGTGGCGCCATGTTG
GATGAGATCCGTCGTGGCGACTGGGTTCCCCGTTCTGTTCATCGAAATAATCGTCGAGTAGCTCAAGCCATTGATGAGTT
AGAGCAAGTGTTGGGTCGTGATGCACGCGACACAGAAATTGCAGAAAAACTTGATATGACGCTCGATGAGTACCATCATA
TTCTTAACGATGTTTCTGTTGGTAAAATCATAGGGATAGAAGACTTAGGCGTATCGCAGGATATACTCACTTCGAGCGAT
AATGTCAGTGATGGTACATATGAAGCGTTAGCAGAAACCCAGTTTCATTCAGCACTAGTTGAGGCGATAAAATTATTGCC
CGAAAGAGATGCGCTAGTGCTATCGTTATACTACGATGAAGCATTGAATTTAAAAGAAATTGGCGCCATTCTTGAGGTAA
GTGAATCGCGAGTTAGCCAGATATTAAGTCAGGCAATGTTGCGGCTCAAAGGCAAACTCAAGCATTGGACATAA

Upstream 100 bases:

>100_bases
AAAATCATGAGCTGGCCAGTACCACAGCAACCTGGTGGGCATTTGGAGTTTTTCGTTGAGCGTTTAGTTCAGCGACCAGA
TTTTCAAGAGGAAAAAACGA

Downstream 100 bases:

>100_bases
GTATAATTATTTATTGAGCACAGAATGTCGGCTCACCGGAGGAAACCTTGGACAAGAATATGAAGATTCTCATCGTTGAT
GACTTTTCGACAATGAGACG

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MNKAAAYTCLDSKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSKFDGGKGAKFETFAGIRIRGAML
DEIRRGDWVPRSVHRNNRRVAQAIDELEQVLGRDARDTEIAEKLDMTLDEYHHILNDVSVGKIIGIEDLGVSQDILTSSD
NVSDGTYEALAETQFHSALVEAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQILSQAMLRLKGKLKHWT

Sequences:

>Translated_237_residues
MNKAAAYTCLDSKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSKFDGGKGAKFETFAGIRIRGAML
DEIRRGDWVPRSVHRNNRRVAQAIDELEQVLGRDARDTEIAEKLDMTLDEYHHILNDVSVGKIIGIEDLGVSQDILTSSD
NVSDGTYEALAETQFHSALVEAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQILSQAMLRLKGKLKHWT
>Mature_237_residues
MNKAAAYTCLDSKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSKFDGGKGAKFETFAGIRIRGAML
DEIRRGDWVPRSVHRNNRRVAQAIDELEQVLGRDARDTEIAEKLDMTLDEYHHILNDVSVGKIIGIEDLGVSQDILTSSD
NVSDGTYEALAETQFHSALVEAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQILSQAMLRLKGKLKHWT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=237, Percent_Identity=49.3670886075949, Blast_Score=210, Evalue=5e-56,
Organism=Escherichia coli, GI1789448, Length=239, Percent_Identity=27.1966527196653, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 26310; Mature: 26310

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAAAYTCLDSKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSKFD
CCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
GGKGAKFETFAGIRIRGAMLDEIRRGDWVPRSVHRNNRRVAQAIDELEQVLGRDARDTEI
CCCCCCEEHCCCCEEHHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCCHHHH
AEKLDMTLDEYHHILNDVSVGKIIGIEDLGVSQDILTSSDNVSDGTYEALAETQFHSALV
HHHHHHHHHHHHHHHHCCCCCCEECCHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHH
EAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQILSQAMLRLKGKLKHWT
HHHHHCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNKAAAYTCLDSKTSIVEQYAPLVKRIAHHLLARLPASVQLDDLLQAGMMGLLEASSKFD
CCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
GGKGAKFETFAGIRIRGAMLDEIRRGDWVPRSVHRNNRRVAQAIDELEQVLGRDARDTEI
CCCCCCEEHCCCCEEHHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCCHHHH
AEKLDMTLDEYHHILNDVSVGKIIGIEDLGVSQDILTSSDNVSDGTYEALAETQFHSALV
HHHHHHHHHHHHHHHHCCCCCCEECCHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHH
EAIKLLPERDALVLSLYYDEALNLKEIGAILEVSESRVSQILSQAMLRLKGKLKHWT
HHHHHCCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]