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The map label for this gene is mscS [H]

Identifier: 117919655

GI number: 117919655

Start: 1410316

End: 1411143

Strand: Reverse

Name: mscS [H]

Synonym: Shewana3_1206

Alternate gene names: 117919655

Gene position: 1411143-1410316 (Counterclockwise)

Preceding gene: 117919656

Following gene: 117919654

Centisome position: 28.38

GC content: 42.87

Gene sequence:

>828_bases
ATGGAAAACCTCGAAGGTTTATTAAAGCAGGCGCCTGATTTGGTCATGACCTATGGCTTAAAAATCTTATTTGCCCTCAT
TATTTTCTTCGTCGGTAAATATTTTTCTGGCGTCGCACAAAAGTTAGTACGTAAGGTACTCAACAGCCGCAAGATTGACC
CAACAGTGGTGTCTTTTGTGGCTAACTTAGCTTGGGCAGTGGTGTTTGTGTTCACCATTATCGCCACCTTAGGCCAAATT
GGCGTACAAACCGCCTCTTTAGTCGCCGTTATCGGTGCCGCGGGTTTAGCCGTTGGTTTAGCACTACAAGGTTCTCTGTC
TAACTTTGCCTCAGGCGTATTAATGGTGCTATTCCGTCCATGCCGCGTAGGTGATTATATTGAAGCGGCGGGTATTGCCG
GTACTGTTGATGAAATCACCATTTTCTCAACCAAATTACGCACCCCAGATAATAAAGTGATTGTGGCGCCAAACTCTTCA
ATCATGAATGGCACTATCACAAACTACTCTGCCTCTGAAAATCGTCGTATCGATTTAGTGATTGGTGTGTCTTATTCTGC
GGATATTGCGCAAACCAAAAAAGTATTAACAGAGATTTTAGATAACAACCAATACGTGCTGAAAGAGCCTGGTTACACCA
TAGGTCTTTCTGAATTGGCAAACTCTTCAATCAACTTTGTGGTTCGTCCTTGGGTTAAAACCGCCGATTACTGGACTGCG
CGTTTCCAAATTTTAGAACAAATCAAAAATGCACTCGATGCAGCGAACATTGAAATTCCATTCCCACAAATGGATATTCA
TGTGAAGCAATTGCCTGAGAGCAAATAA

Upstream 100 bases:

>100_bases
GCGGATAATCATTCCAGCGAAGGTCGCAGATTAAATCGACGAGTACAATTTATCGTCAATAATAACTAGTTTTGTTAGCG
TATTTTTAGGGAGTAATTTA

Downstream 100 bases:

>100_bases
TCAGCAAGCATATTAAAAGGCCGGTGAATACCGGCCTTTTTTATGGTGATAGTTTAGGTTTTGTCGTACCTTAAGCCAAT
GTGCACAACATACCTGAGTA

Product: mechanosensitive ion channel protein MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MENLEGLLKQAPDLVMTYGLKILFALIIFFVGKYFSGVAQKLVRKVLNSRKIDPTVVSFVANLAWAVVFVFTIIATLGQI
GVQTASLVAVIGAAGLAVGLALQGSLSNFASGVLMVLFRPCRVGDYIEAAGIAGTVDEITIFSTKLRTPDNKVIVAPNSS
IMNGTITNYSASENRRIDLVIGVSYSADIAQTKKVLTEILDNNQYVLKEPGYTIGLSELANSSINFVVRPWVKTADYWTA
RFQILEQIKNALDAANIEIPFPQMDIHVKQLPESK

Sequences:

>Translated_275_residues
MENLEGLLKQAPDLVMTYGLKILFALIIFFVGKYFSGVAQKLVRKVLNSRKIDPTVVSFVANLAWAVVFVFTIIATLGQI
GVQTASLVAVIGAAGLAVGLALQGSLSNFASGVLMVLFRPCRVGDYIEAAGIAGTVDEITIFSTKLRTPDNKVIVAPNSS
IMNGTITNYSASENRRIDLVIGVSYSADIAQTKKVLTEILDNNQYVLKEPGYTIGLSELANSSINFVVRPWVKTADYWTA
RFQILEQIKNALDAANIEIPFPQMDIHVKQLPESK
>Mature_275_residues
MENLEGLLKQAPDLVMTYGLKILFALIIFFVGKYFSGVAQKLVRKVLNSRKIDPTVVSFVANLAWAVVFVFTIIATLGQI
GVQTASLVAVIGAAGLAVGLALQGSLSNFASGVLMVLFRPCRVGDYIEAAGIAGTVDEITIFSTKLRTPDNKVIVAPNSS
IMNGTITNYSASENRRIDLVIGVSYSADIAQTKKVLTEILDNNQYVLKEPGYTIGLSELANSSINFVVRPWVKTADYWTA
RFQILEQIKNALDAANIEIPFPQMDIHVKQLPESK

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=262, Percent_Identity=48.4732824427481, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1786670, Length=257, Percent_Identity=24.124513618677, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787591, Length=204, Percent_Identity=29.4117647058824, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI2367355, Length=194, Percent_Identity=24.7422680412371, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 29915; Mature: 29915

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLEGLLKQAPDLVMTYGLKILFALIIFFVGKYFSGVAQKLVRKVLNSRKIDPTVVSFV
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ANLAWAVVFVFTIIATLGQIGVQTASLVAVIGAAGLAVGLALQGSLSNFASGVLMVLFRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
CRVGDYIEAAGIAGTVDEITIFSTKLRTPDNKVIVAPNSSIMNGTITNYSASENRRIDLV
CCCCCHHHHCCCCCCHHHEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEE
IGVSYSADIAQTKKVLTEILDNNQYVLKEPGYTIGLSELANSSINFVVRPWVKTADYWTA
EECCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEHHHHCCCCCCEEEECCHHHHHHHHH
RFQILEQIKNALDAANIEIPFPQMDIHVKQLPESK
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCC
>Mature Secondary Structure
MENLEGLLKQAPDLVMTYGLKILFALIIFFVGKYFSGVAQKLVRKVLNSRKIDPTVVSFV
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ANLAWAVVFVFTIIATLGQIGVQTASLVAVIGAAGLAVGLALQGSLSNFASGVLMVLFRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
CRVGDYIEAAGIAGTVDEITIFSTKLRTPDNKVIVAPNSSIMNGTITNYSASENRRIDLV
CCCCCHHHHCCCCCCHHHEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEE
IGVSYSADIAQTKKVLTEILDNNQYVLKEPGYTIGLSELANSSINFVVRPWVKTADYWTA
EECCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEHHHHCCCCCCEEEECCHHHHHHHHH
RFQILEQIKNALDAANIEIPFPQMDIHVKQLPESK
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]