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The map label for this gene is acrA [C]

Identifier: 117919533

GI number: 117919533

Start: 1274203

End: 1275315

Strand: Reverse

Name: acrA [C]

Synonym: Shewana3_1084

Alternate gene names: 117919533

Gene position: 1275315-1274203 (Counterclockwise)

Preceding gene: 117919534

Following gene: 117919532

Centisome position: 25.65

GC content: 51.48

Gene sequence:

>1113_bases
ATGAAAAAAACCATAATAGCCATTGCCTTAATTGCTGCGGTTGCCACCGCTGTGAGTTTTAGTGATGTACTGCAGGCGGC
CAAACCCGAAAGCGCGCCCCATTTAATGCGCACTGTACCTGTGGTCACAGGAGAAGTGGTTGAACATCCACTGGCACAAT
CGATTTCACTCATAGGTAAACTCGCCGCCGATCGCGCCGTGGTTATCGCGCCGCAAGTCACAGGCAAAATTAAGCAAATC
GCCGTGACATCGAATCAGGCGGTCAAAAAAGGCCAACTGCTTATCGAACTCGATGATATGAAAGCCCAGGCCGCAGTAGC
CGAGGCCAATGCCTTTTTAAATGATGAAACCCGTAAACTCAGGGAATTTGAAAAACTCATCAGCCGCAACGCCATCACCC
AAACCGAAATCGATGCGCAAAAGGCCAGCGTCGATATCGCCAGAGCCCGACTCGCCTCGGCGCAGGCAGATTTACATTAC
CATTCGCTAATCGCTCCCTTTGCTGGCAAAACCGGCCTGATTAACTTCAGTGAAGGAAAAATGGTCAGCATTGGCACAGA
GCTGATGACCTTGGATGATTTATCCAGCATGCGACTCGACCTACAAGTGCCCGAACATTTTCTGTCACAGCTCAGCATTG
GCATGCCCGTCTCGGCGACCAGCCGCGCCTGGCCGGGAGAAACCTTTATCGGCAAAGTAGTCGCCATCGATCCCCGCGTC
AATGAAGAAACCTTAAACTTGAAGATCCGCGTACAATTTGAAAACGCGAAAAACCGTTTAAAGCCGGGCATGATGATGTC
ATCGACCCTCACCTTCCCGCCGATTTCCGCCCCGATTGTGCCAGTGCAAGCCTTAGAATATTCAGGCACTAAGCGCTATG
TGTATGTGGTGGGTGAGGACCATATCGCCCATCGCACCGAAGTAATTTTAGGTGCCCGCGTGGGTGACCAAGTGCTTATT
GATTCGGGCCTCAAGATTGGCGACAAAGTGGTGGTGCAAGGGCTAGTGAACATGCGCGATGGCTTAAAGATCAATGAAGT
GGTGCTCGAAGCCAAAAACGATGCTAACCGTCCAGCGGCCAAGTCTGACGTCAACACCGCGGAGACGAAATAA

Upstream 100 bases:

>100_bases
AACTTTACAAAGTTTTACTCACCTGATGACGGTTATTCACTAGAATAGCGCCATTAGTGCGATCGCCTCTTAACCGTCTT
TGTGCGAGATAATTATTCCA

Downstream 100 bases:

>100_bases
TGTGGCTGTCCGATGTATCCGTTAAGCGCCCCGTCGTCGCGATCGTATTAAGCTTATTGCTGTGCGTCTTTGGTTTAGTA
TCCTTCACTAAACTGTCGGT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKKTIIAIALIAAVATAVSFSDVLQAAKPESAPHLMRTVPVVTGEVVEHPLAQSISLIGKLAADRAVVIAPQVTGKIKQI
AVTSNQAVKKGQLLIELDDMKAQAAVAEANAFLNDETRKLREFEKLISRNAITQTEIDAQKASVDIARARLASAQADLHY
HSLIAPFAGKTGLINFSEGKMVSIGTELMTLDDLSSMRLDLQVPEHFLSQLSIGMPVSATSRAWPGETFIGKVVAIDPRV
NEETLNLKIRVQFENAKNRLKPGMMMSSTLTFPPISAPIVPVQALEYSGTKRYVYVVGEDHIAHRTEVILGARVGDQVLI
DSGLKIGDKVVVQGLVNMRDGLKINEVVLEAKNDANRPAAKSDVNTAETK

Sequences:

>Translated_370_residues
MKKTIIAIALIAAVATAVSFSDVLQAAKPESAPHLMRTVPVVTGEVVEHPLAQSISLIGKLAADRAVVIAPQVTGKIKQI
AVTSNQAVKKGQLLIELDDMKAQAAVAEANAFLNDETRKLREFEKLISRNAITQTEIDAQKASVDIARARLASAQADLHY
HSLIAPFAGKTGLINFSEGKMVSIGTELMTLDDLSSMRLDLQVPEHFLSQLSIGMPVSATSRAWPGETFIGKVVAIDPRV
NEETLNLKIRVQFENAKNRLKPGMMMSSTLTFPPISAPIVPVQALEYSGTKRYVYVVGEDHIAHRTEVILGARVGDQVLI
DSGLKIGDKVVVQGLVNMRDGLKINEVVLEAKNDANRPAAKSDVNTAETK
>Mature_370_residues
MKKTIIAIALIAAVATAVSFSDVLQAAKPESAPHLMRTVPVVTGEVVEHPLAQSISLIGKLAADRAVVIAPQVTGKIKQI
AVTSNQAVKKGQLLIELDDMKAQAAVAEANAFLNDETRKLREFEKLISRNAITQTEIDAQKASVDIARARLASAQADLHY
HSLIAPFAGKTGLINFSEGKMVSIGTELMTLDDLSSMRLDLQVPEHFLSQLSIGMPVSATSRAWPGETFIGKVVAIDPRV
NEETLNLKIRVQFENAKNRLKPGMMMSSTLTFPPISAPIVPVQALEYSGTKRYVYVVGEDHIAHRTEVILGARVGDQVLI
DSGLKIGDKVVVQGLVNMRDGLKINEVVLEAKNDANRPAAKSDVNTAETK

Specific function: Acrab Is A Drug Efflux Protein With A Broad Substrate Specificity. [C]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=387, Percent_Identity=26.3565891472868, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081796, Length=362, Percent_Identity=27.0718232044199, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1789665, Length=344, Percent_Identity=27.3255813953488, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789929, Length=305, Percent_Identity=23.9344262295082, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87081951, Length=200, Percent_Identity=30.5, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39910; Mature: 39910

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTIIAIALIAAVATAVSFSDVLQAAKPESAPHLMRTVPVVTGEVVEHPLAQSISLIGK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHH
LAADRAVVIAPQVTGKIKQIAVTSNQAVKKGQLLIELDDMKAQAAVAEANAFLNDETRKL
HHCCCEEEEECCCCCCEEEEEEECCCHHCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHH
REFEKLISRNAITQTEIDAQKASVDIARARLASAQADLHYHSLIAPFAGKTGLINFSEGK
HHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCEEECCCCC
MVSIGTELMTLDDLSSMRLDLQVPEHFLSQLSIGMPVSATSRAWPGETFIGKVVAIDPRV
EEEECCCEEEHHCCCCCEEEEECHHHHHHHHCCCCCCCCCCCCCCCCHHEEEEEEECCCC
NEETLNLKIRVQFENAKNRLKPGMMMSSTLTFPPISAPIVPVQALEYSGTKRYVYVVGED
CCCEEEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCEEEEEEECCC
HIAHRTEVILGARVGDQVLIDSGLKIGDKVVVQGLVNMRDGLKINEVVLEAKNDANRPAA
CHHHHHEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEHEEEECCCCCCCCCC
KSDVNTAETK
CCCCCCCCCC
>Mature Secondary Structure
MKKTIIAIALIAAVATAVSFSDVLQAAKPESAPHLMRTVPVVTGEVVEHPLAQSISLIGK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHH
LAADRAVVIAPQVTGKIKQIAVTSNQAVKKGQLLIELDDMKAQAAVAEANAFLNDETRKL
HHCCCEEEEECCCCCCEEEEEEECCCHHCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHH
REFEKLISRNAITQTEIDAQKASVDIARARLASAQADLHYHSLIAPFAGKTGLINFSEGK
HHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCEEECCCCC
MVSIGTELMTLDDLSSMRLDLQVPEHFLSQLSIGMPVSATSRAWPGETFIGKVVAIDPRV
EEEECCCEEEHHCCCCCEEEEECHHHHHHHHCCCCCCCCCCCCCCCCHHEEEEEEECCCC
NEETLNLKIRVQFENAKNRLKPGMMMSSTLTFPPISAPIVPVQALEYSGTKRYVYVVGED
CCCEEEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCEEEEEEECCC
HIAHRTEVILGARVGDQVLIDSGLKIGDKVVVQGLVNMRDGLKINEVVLEAKNDANRPAA
CHHHHHEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEHEEEECCCCCCCCCC
KSDVNTAETK
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]