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The map label for this gene is truB

Identifier: 117919481

GI number: 117919481

Start: 1205386

End: 1206339

Strand: Direct

Name: truB

Synonym: Shewana3_1032

Alternate gene names: 117919481

Gene position: 1205386-1206339 (Clockwise)

Preceding gene: 117919480

Following gene: 117919482

Centisome position: 24.24

GC content: 51.05

Gene sequence:

>954_bases
ATGGCGAGACGTTCGAAGGGCCGCTTTATCGACGGTATTGTGTTGTTGGACAAAGCCACTGGCATGAGTTCCAACTTTGC
ATTACAACGGGTAAAGCGCTTTTTTAATGCCAATAAAGCGGGGCACACGGGGGCGCTCGATCCCTTGGCAACGGGTATGC
TACCTGTGTGTTTAGGCGAAGCGACTAAGTTTTCGCAGCATTTGCTGGATTCGGATAAGCGTTATCTGGTTACCGCAAAA
CTGGGACAACGCACAGATACTAGCGACTCCGACGGTGAAGTGGTGCAGACTCGCCCGCTCGAATTCACTGAGGCGCAGTT
AATGTCCGCCTTAGAGCATTTTCGTGGCGATACCCAGCAAGTACCCTCCATGTATTCGGCGCTGAAATACCAAGGCCAGC
CTTTGTATAAGTATGCCCGCGAGGGGATTGAAGTGCCGCGCGAGGCGCGTCCAATTACGGTCTTTGAACTGAACTTCATC
GGTCTAGAAGGCGATGAGCTGACCTTAGACATTCATTGTTCAAAGGGCACTTATATTCGCACTATCATCGATGATTTAGG
TGAGATGCTTGGTTGTGGCGCCCATGTGATTATGCTGCGTCGTACTCAAGTCGCGCATTATCCCTACGACAAAATGGTCA
CCTTGGAGCAACTCGAAGCCTTAGTCGCTAAGGCGCAGGAAGAGCAGCTTGACCCTAGCAGTTTGCTTGACTCGTTGCTG
CTACCTATGGATACCGCTGTTGCTGACTTTCCTGAGGTCAATGTGCCAGATGCAAGCGCAGCTTACTTAATGCAAGGTCA
GGCGGTACGTGTCTCTGGACTCGTTGCAGATACATTGGTGCGCATTACGTTGGGCACTGAGCGTCGTTTCGTTGGAATTG
GCGAGATGAACCAAGATGGCTTACTGGCACCAAAGCGCTTAGTTGTGCTCCACGACCAAGCTAAAGCATCTTAA

Upstream 100 bases:

>100_bases
TGATCAACAGTGATAAAGCCAAGCAGCAACAGTTTGGTAGCGCCGAAGATGTGACGTCGAATGACATCGACGAAGCCGAT
GACACCGAAGGTAAAGCGTA

Downstream 100 bases:

>100_bases
TTGATTGTCTTGGGTTATTAATCCATCGCATTTATTGCGCGCGCCAAGGATTATGGGTAGAATAGCGCGCCCTCTGGCTG
AGTTAGTGATCGGCTAGAGA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MARRSKGRFIDGIVLLDKATGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPVCLGEATKFSQHLLDSDKRYLVTAK
LGQRTDTSDSDGEVVQTRPLEFTEAQLMSALEHFRGDTQQVPSMYSALKYQGQPLYKYAREGIEVPREARPITVFELNFI
GLEGDELTLDIHCSKGTYIRTIIDDLGEMLGCGAHVIMLRRTQVAHYPYDKMVTLEQLEALVAKAQEEQLDPSSLLDSLL
LPMDTAVADFPEVNVPDASAAYLMQGQAVRVSGLVADTLVRITLGTERRFVGIGEMNQDGLLAPKRLVVLHDQAKAS

Sequences:

>Translated_317_residues
MARRSKGRFIDGIVLLDKATGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPVCLGEATKFSQHLLDSDKRYLVTAK
LGQRTDTSDSDGEVVQTRPLEFTEAQLMSALEHFRGDTQQVPSMYSALKYQGQPLYKYAREGIEVPREARPITVFELNFI
GLEGDELTLDIHCSKGTYIRTIIDDLGEMLGCGAHVIMLRRTQVAHYPYDKMVTLEQLEALVAKAQEEQLDPSSLLDSLL
LPMDTAVADFPEVNVPDASAAYLMQGQAVRVSGLVADTLVRITLGTERRFVGIGEMNQDGLLAPKRLVVLHDQAKAS
>Mature_316_residues
ARRSKGRFIDGIVLLDKATGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPVCLGEATKFSQHLLDSDKRYLVTAKL
GQRTDTSDSDGEVVQTRPLEFTEAQLMSALEHFRGDTQQVPSMYSALKYQGQPLYKYAREGIEVPREARPITVFELNFIG
LEGDELTLDIHCSKGTYIRTIIDDLGEMLGCGAHVIMLRRTQVAHYPYDKMVTLEQLEALVAKAQEEQLDPSSLLDSLLL
PMDTAVADFPEVNVPDASAAYLMQGQAVRVSGLVADTLVRITLGTERRFVGIGEMNQDGLLAPKRLVVLHDQAKAS

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=185, Percent_Identity=37.8378378378378, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4503337, Length=275, Percent_Identity=25.8181818181818, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI215599015, Length=275, Percent_Identity=25.8181818181818, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI2367200, Length=310, Percent_Identity=64.5161290322581, Blast_Score=422, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17553978, Length=328, Percent_Identity=25, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=309, Percent_Identity=27.1844660194175, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324037, Length=147, Percent_Identity=37.4149659863946, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281364189, Length=265, Percent_Identity=26.0377358490566, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364187, Length=265, Percent_Identity=26.0377358490566, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364185, Length=265, Percent_Identity=26.0377358490566, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364183, Length=265, Percent_Identity=26.0377358490566, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI62471759, Length=265, Percent_Identity=26.0377358490566, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17975520, Length=265, Percent_Identity=26.0377358490566, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_SHESA (A0KTZ8)

Other databases:

- EMBL:   CP000469
- RefSeq:   YP_868673.1
- ProteinModelPortal:   A0KTZ8
- SMR:   A0KTZ8
- STRING:   A0KTZ8
- GeneID:   4479377
- GenomeReviews:   CP000469_GR
- KEGG:   shn:Shewana3_1032
- NMPDR:   fig|94122.5.peg.1155
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   A0KTZ8
- ProtClustDB:   PRK05033
- BioCyc:   SSP94122:SHEWANA3_1032-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 34945; Mature: 34813

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: ACT_SITE 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRSKGRFIDGIVLLDKATGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPVCLGE
CCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHCH
ATKFSQHLLDSDKRYLVTAKLGQRTDTSDSDGEVVQTRPLEFTEAQLMSALEHFRGDTQQ
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCHHH
VPSMYSALKYQGQPLYKYAREGIEVPREARPITVFELNFIGLEGDELTLDIHCSKGTYIR
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCEEEEEEEECCCCCHH
TIIDDLGEMLGCGAHVIMLRRTQVAHYPYDKMVTLEQLEALVAKAQEEQLDPSSLLDSLL
HHHHHHHHHHCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LPMDTAVADFPEVNVPDASAAYLMQGQAVRVSGLVADTLVRITLGTERRFVGIGEMNQDG
HCHHHHHHCCCCCCCCCCCHHHHHCCCEEEEHHHHHHHEEEEEECCCEEEEECCCCCCCC
LLAPKRLVVLHDQAKAS
CCCCCEEEEEECCCCCC
>Mature Secondary Structure 
ARRSKGRFIDGIVLLDKATGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPVCLGE
CCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHCH
ATKFSQHLLDSDKRYLVTAKLGQRTDTSDSDGEVVQTRPLEFTEAQLMSALEHFRGDTQQ
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCHHH
VPSMYSALKYQGQPLYKYAREGIEVPREARPITVFELNFIGLEGDELTLDIHCSKGTYIR
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCEEEEEEEECCCCCHH
TIIDDLGEMLGCGAHVIMLRRTQVAHYPYDKMVTLEQLEALVAKAQEEQLDPSSLLDSLL
HHHHHHHHHHCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LPMDTAVADFPEVNVPDASAAYLMQGQAVRVSGLVADTLVRITLGTERRFVGIGEMNQDG
HCHHHHHHCCCCCCCCCCCHHHHHCCCEEEEHHHHHHHEEEEEECCCEEEEECCCCCCCC
LLAPKRLVVLHDQAKAS
CCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA