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The map label for this gene is rbfA [H]

Identifier: 117919480

GI number: 117919480

Start: 1204946

End: 1205386

Strand: Direct

Name: rbfA [H]

Synonym: Shewana3_1031

Alternate gene names: 117919480

Gene position: 1204946-1205386 (Clockwise)

Preceding gene: 117919479

Following gene: 117919481

Centisome position: 24.23

GC content: 46.71

Gene sequence:

>441_bases
ATGGCAAAAGAATTCAGTCGTACACGTCGCATAGCCCAGCAGTTGCAGCAAGAGCTGGCTGTTGTGTTACAACGCGACAT
GAAAGATCCCCGTATTGGTTTTGTAACCGTTAATGATGTCGATGTATCCCGCGATTTAAGTTACGCCAAAGTGTTTGTGA
CTTTCTTCGAAGAAGACAAAGAGCTGGTGCAAGAAAAGCTCAATGCCTTGATTACAGCTGCGCCTTATATCCGTACTTTA
GTTGCGGGCCGCATGAAGCTGAGAGTCATGCCTGAAATCCGTTTCGTGTATGACAGTTCATTGGTTGAAGGTATGCGTAT
GTCTAACCTCGTCAGCCAAGTGATCAACAGTGATAAAGCCAAGCAGCAACAGTTTGGTAGCGCCGAAGATGTGACGTCGA
ATGACATCGACGAAGCCGATGACACCGAAGGTAAAGCGTAA

Upstream 100 bases:

>100_bases
AAGTATTCGAAACCGTCGAAGTGGCACGTACACTGTAACGATAAAGAGGGCGGCATTAGCCGCCCTTTCAATTTAATACA
TTAGCTATGGTAACTCTATT

Downstream 100 bases:

>100_bases
TGGCGAGACGTTCGAAGGGCCGCTTTATCGACGGTATTGTGTTGTTGGACAAAGCCACTGGCATGAGTTCCAACTTTGCA
TTACAACGGGTAAAGCGCTT

Product: ribosome-binding factor A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 145

Protein sequence:

>146_residues
MAKEFSRTRRIAQQLQQELAVVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVFVTFFEEDKELVQEKLNALITAAPYIRTL
VAGRMKLRVMPEIRFVYDSSLVEGMRMSNLVSQVINSDKAKQQQFGSAEDVTSNDIDEADDTEGKA

Sequences:

>Translated_146_residues
MAKEFSRTRRIAQQLQQELAVVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVFVTFFEEDKELVQEKLNALITAAPYIRTL
VAGRMKLRVMPEIRFVYDSSLVEGMRMSNLVSQVINSDKAKQQQFGSAEDVTSNDIDEADDTEGKA
>Mature_145_residues
AKEFSRTRRIAQQLQQELAVVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVFVTFFEEDKELVQEKLNALITAAPYIRTLV
AGRMKLRVMPEIRFVYDSSLVEGMRMSNLVSQVINSDKAKQQQFGSAEDVTSNDIDEADDTEGKA

Specific function: Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Essential for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA [H]

COG id: COG0858

COG function: function code J; Ribosome-binding factor A

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rbfA family [H]

Homologues:

Organism=Escherichia coli, GI1789558, Length=124, Percent_Identity=51.6129032258064, Blast_Score=136, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015946
- InterPro:   IPR000238
- InterPro:   IPR020053
- ProDom:   PD007327 [H]

Pfam domain/function: PF02033 RBFA [H]

EC number: NA

Molecular weight: Translated: 16596; Mature: 16465

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS01319 RBFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEFSRTRRIAQQLQQELAVVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVFVTFFEEDK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
ELVQEKLNALITAAPYIRTLVAGRMKLRVMPEIRFVYDSSLVEGMRMSNLVSQVINSDKA
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
KQQQFGSAEDVTSNDIDEADDTEGKA
HHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKEFSRTRRIAQQLQQELAVVLQRDMKDPRIGFVTVNDVDVSRDLSYAKVFVTFFEEDK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
ELVQEKLNALITAAPYIRTLVAGRMKLRVMPEIRFVYDSSLVEGMRMSNLVSQVINSDKA
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
KQQQFGSAEDVTSNDIDEADDTEGKA
HHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]