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The map label for this gene is proA [H]

Identifier: 117919404

GI number: 117919404

Start: 1120622

End: 1121899

Strand: Direct

Name: proA [H]

Synonym: Shewana3_0955

Alternate gene names: 117919404

Gene position: 1120622-1121899 (Clockwise)

Preceding gene: 117919403

Following gene: 117919408

Centisome position: 22.54

GC content: 53.21

Gene sequence:

>1278_bases
TTGAGTCAAACTAATCAAGCGCAATATTTACAACAACTGGGCAGTCAGGCTAAGCAAGCCAGCTATGCCTTGGCCAATCT
AAGCGCCGTACAGAAAGCGGATTTACTCGAGGCGATTGCCGAGGCCCTGACGCAGAACACTCAGGCGATTCTTGCGGCTA
ACGCCAAAGATGTTGCCGCTGCTAAGGCTGAAGGCTTGAATGATGCGATGATCGATAGATTGTTGCTTAACGAGTCGCGT
CTGGCGGGGATTATCGGTGATATCGGCGATGTGGTGCGTCTTGCCGATCCCGTTGGCGAAGAGTTTGGCAGCCGCGTGTT
GGACAATGGGTTAAGGCTCACGCGCCGCCGCGTACCGCTGGGGGTAATTGGGGTTATTTATGAGGCGCGGCCGAATGTGA
CGGTCGATATTGCGGTATTGGCACTGAAAACCGGTAACGCAGTGATTTTGCGCGGCGGAAAAGAGACGCTCGAATCTAAC
AAACTGATTAGTGAAGTGATCCGTGGCGCGATTGCGAGCCAAGGTCTGCCAGTCGATGCGGTGCAGCTGATTGATTCTCC
CGATAGAGCCTTAGTCACAGGTCTACTCAAGCTCGACCAATATGTCGATATGATAGTCCCCCGTGGCGGACAAGCACTGC
AGCGTCTTTGCGCCGAGCAAGCGACGATTCCGGTGATTTTAGGCGGCATAGGGATTTGCCATCTGTATGTCGATAAACAT
GCCGATCTTGAGCGCGCACTCGAGGTGATTGCTAACGCAAAAGTGCAGCGCCCCACGGTGTGTAATGCGTTAGACACCTT
GCTGGTGGATACCGCGGTTGCCGAGCGCTTTGTGCCGCAAATTGCCGAGTACTTACACCGCTTAGGTGTGCGTTTTTCGG
TGTGTGAACAAAGCTATGCGCTGCTCGATGGGCTGGGTTTTGATATTTCCCCAGCAACAGAGCAAAGCTTTGCGACCGAA
TGGCTGTCTTTGACCTTAGGCATTAAAGTGGTGAGCGATATAGATACAGCCATTGCCCATATCCGTACCTATTCCAGTGG
GCACTCTGAGGCGATTCTCACCGATGATATCCACGTGGCAACGCATTTTATGAACGAAGTGAATTCGGCTGCTGTGTATG
TGAATGCCAGTACCCGCTTTACCGATGGCGGCCAATTTGGCTTGGGTGCGGAGGTGGCCGTGAGCACACAAAAACTCCAT
GCCCGTGGTCCTATGGGGCTTGAGGCGCTGACCACCTATAAGTGGTTAGCATGGGGTGATTACACTAGCCGCGCTTAG

Upstream 100 bases:

>100_bases
GGGTAAACATTCCGACGAAATCGAGTCAGTGCTCGGTTACGATTATGGTGACGCAATAGTGCACCGCAACGACATGGTCG
TCTTATAAGCGAGGTCTGTT

Downstream 100 bases:

>100_bases
TCCTTATCGTTTTATCAAATCGTTCCATTAAAAAAGCCGCAATAGCGGCTTTTTTTGTTGGCAAATATCTCGGTTAATGG
ATAAGCGGGGTGTAAGTGGC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSQTNQAQYLQQLGSQAKQASYALANLSAVQKADLLEAIAEALTQNTQAILAANAKDVAAAKAEGLNDAMIDRLLLNESR
LAGIIGDIGDVVRLADPVGEEFGSRVLDNGLRLTRRRVPLGVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLESN
KLISEVIRGAIASQGLPVDAVQLIDSPDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKH
ADLERALEVIANAKVQRPTVCNALDTLLVDTAVAERFVPQIAEYLHRLGVRFSVCEQSYALLDGLGFDISPATEQSFATE
WLSLTLGIKVVSDIDTAIAHIRTYSSGHSEAILTDDIHVATHFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
ARGPMGLEALTTYKWLAWGDYTSRA

Sequences:

>Translated_425_residues
MSQTNQAQYLQQLGSQAKQASYALANLSAVQKADLLEAIAEALTQNTQAILAANAKDVAAAKAEGLNDAMIDRLLLNESR
LAGIIGDIGDVVRLADPVGEEFGSRVLDNGLRLTRRRVPLGVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLESN
KLISEVIRGAIASQGLPVDAVQLIDSPDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKH
ADLERALEVIANAKVQRPTVCNALDTLLVDTAVAERFVPQIAEYLHRLGVRFSVCEQSYALLDGLGFDISPATEQSFATE
WLSLTLGIKVVSDIDTAIAHIRTYSSGHSEAILTDDIHVATHFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
ARGPMGLEALTTYKWLAWGDYTSRA
>Mature_424_residues
SQTNQAQYLQQLGSQAKQASYALANLSAVQKADLLEAIAEALTQNTQAILAANAKDVAAAKAEGLNDAMIDRLLLNESRL
AGIIGDIGDVVRLADPVGEEFGSRVLDNGLRLTRRRVPLGVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLESNK
LISEVIRGAIASQGLPVDAVQLIDSPDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKHA
DLERALEVIANAKVQRPTVCNALDTLLVDTAVAERFVPQIAEYLHRLGVRFSVCEQSYALLDGLGFDISPATEQSFATEW
LSLTLGIKVVSDIDTAIAHIRTYSSGHSEAILTDDIHVATHFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHA
RGPMGLEALTTYKWLAWGDYTSRA

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=413, Percent_Identity=37.046004842615, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI21361368, Length=413, Percent_Identity=37.046004842615, Blast_Score=253, Evalue=2e-67,
Organism=Escherichia coli, GI1786438, Length=416, Percent_Identity=58.8942307692308, Blast_Score=490, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17569969, Length=416, Percent_Identity=36.0576923076923, Blast_Score=261, Evalue=5e-70,
Organism=Caenorhabditis elegans, GI17569967, Length=416, Percent_Identity=36.0576923076923, Blast_Score=261, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6324899, Length=431, Percent_Identity=38.7470997679814, Blast_Score=288, Evalue=1e-78,
Organism=Drosophila melanogaster, GI21357643, Length=421, Percent_Identity=35.6294536817102, Blast_Score=256, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 45520; Mature: 45388

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTNQAQYLQQLGSQAKQASYALANLSAVQKADLLEAIAEALTQNTQAILAANAKDVAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHH
AKAEGLNDAMIDRLLLNESRLAGIIGDIGDVVRLADPVGEEFGSRVLDNGLRLTRRRVPL
HHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCE
GVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLESNKLISEVIRGAIASQGLPVDA
EEEEEEEECCCCCEEEEEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHH
VQLIDSPDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKH
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCEEEEEEECCC
ADLERALEVIANAKVQRPTVCNALDTLLVDTAVAERFVPQIAEYLHRLGVRFSVCEQSYA
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LLDGLGFDISPATEQSFATEWLSLTLGIKVVSDIDTAIAHIRTYSSGHSEAILTDDIHVA
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHH
THFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKWLAWGD
HHHHHHCCCEEEEEECCCEECCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHHHHHEEECC
YTSRA
CCCCC
>Mature Secondary Structure 
SQTNQAQYLQQLGSQAKQASYALANLSAVQKADLLEAIAEALTQNTQAILAANAKDVAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHH
AKAEGLNDAMIDRLLLNESRLAGIIGDIGDVVRLADPVGEEFGSRVLDNGLRLTRRRVPL
HHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCE
GVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLESNKLISEVIRGAIASQGLPVDA
EEEEEEEECCCCCEEEEEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHH
VQLIDSPDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKH
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCEEEEEEECCC
ADLERALEVIANAKVQRPTVCNALDTLLVDTAVAERFVPQIAEYLHRLGVRFSVCEQSYA
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LLDGLGFDISPATEQSFATEWLSLTLGIKVVSDIDTAIAHIRTYSSGHSEAILTDDIHVA
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHH
THFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKWLAWGD
HHHHHHCCCEEEEEECCCEECCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHHHHHEEECC
YTSRA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]