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The map label for this gene is pepA [H]

Identifier: 117919400

GI number: 117919400

Start: 1112972

End: 1114510

Strand: Direct

Name: pepA [H]

Synonym: Shewana3_0951

Alternate gene names: 117919400

Gene position: 1112972-1114510 (Clockwise)

Preceding gene: 117919398

Following gene: 117919401

Centisome position: 22.38

GC content: 53.02

Gene sequence:

>1539_bases
ATGTTTCAAGTTAACTTTGTTGATGTACAAGCAGAAAATGCCATTTTTGATGGCGAAGGTTGGGATGCGGTTGTGGTGGT
CACTCCGGATCTCGTTTCTATCGGAATTGATGAGATCAGTTTATTGGCCGAGCATGGCGCTAAGGTCGATAAGCGTGTCG
GTAAGAGCCCGACGTTACTGTTTGCCCCCGGTTTAGCCGGTGGCCGTTTAATCATAGCGCCCGTCACTCAAGTGGCTGAT
GATTATGCCGATGTGCGTATTTATGGCGATGCGGCAAGAGCCGCGATTGCCATCGCTAAAGACGCGGGGGCTAAGCGCCC
CCTACTGTATGTGGTGCCTTCTGCCACGCCTAAGTTTGGTTTTGCGAGTGAAGTTGCGGCGCTCGCCTGCGGCCAAGAAT
TATGGCAAACCTTAGAATTGCGCGAGGCAACGGGCTTAACGCCAGCCTTTGAAGCCATAGGGTTGTTGTCTTTATCATCT
GAAAAGAGCCACTTGCTCAACGCATTAGAAGCTGGCCGTGTATTGGCTCGGGATTTATGTGGCACTGAACCTGAGCGCAT
GTCGGCTAAGGCTTTTGCCGAATATTGTTTGCAAGCCTTTAAAGGCAGCGTGATTAAAACCGCAGTGGTGGAAGATAGGG
ATATTCTCGAGCGAGATTATCCGCTGTTAAGTGCGGTGGCGCGTTCTTCCTTTGCCGTTGGCCGGCATCAACCGCGGGTG
GTGAAACTCGAATATCAGCCCGAAGGTGAGATTACTCGTACCTTCTTATTTGCTGGTAAAGGTGTGGTCTACGATACGGG
CGGTGCCGATCTGAAAGTCGGCGGCGCAATGGCCGGCATGAGCCGCGATAAAGGCGGCGCCTCTGCCGTGGCGGGGTTAT
TTAAAACCTTGTCTATGCTCAAGCCTAAAGGGATCCGAGTGATCGCCGAACTGGGCTTAGTGCGTAATAGCATTGGCAGC
GAAGCCTTTGTCACCGATGAAATTATCACTAGCCATGCGGGCGTGCGGGTACGCATTGGTAATACGGATGCGGAAGGGCG
CTTAGTCTTGGCAGACCTGTTAAGTCATCTGCGCATTAAAGCGGTCTCGGCGGTGCAACCAGAGCTGTTTTCGGTCGCGA
CGCTGACGGGTCACGTGGTGCGTTGTTACGGGTCGTATCCTGCTGCGGTTGAAAATGCGGTCGCCAGTGGCGCCCAGTGT
GCGCAGACACTCGCGTTACAGGCGAGCCAATGGGGCGAACCCTTTGAGGTTTCTACATTACGTCGCGATGACTTTGCCAA
GATCCGCGAAGTGTCGGGGGCGGCCGATATTTTATCTTCTAACAATGCGCCATCTTCGGTGACGGCCCGTGGGCATCAAT
ATCCTGCGGCGTTTCTGCTCAAGGCTTCGGGATTGTTTGATCACGGTATTAACGCCGCAATTCCTTTAGCTTACACACAC
TTAGATATCGCAGGCAGTGCCACTGAGGGCGATCCGCTCTATGGTAAACCCACTGCGAGTCCCTTAGTCGGTCTGTATCA
ATATCTGATCAATCGTTAA

Upstream 100 bases:

>100_bases
CCAGCCTCAGTCTTTTCGATGGGGACAAAGGGATTTTTTGATTGGTAATTAAGCGTTATAGCGGGAATAATGCAGGCACA
ATAATGAAAAAGGATGGGTC

Downstream 100 bases:

>100_bases
CAATTTAACGTAATAAATAGAAATAATTCTGTAATATCTTACTTATTAGGTGGTTACAGGGTTATTTCTGTTTTTATGTG
ACGCAGGTCGCCAAGATTTT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Leucine aminopeptidase; LAP; Leucyl aminopeptidase [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MFQVNFVDVQAENAIFDGEGWDAVVVVTPDLVSIGIDEISLLAEHGAKVDKRVGKSPTLLFAPGLAGGRLIIAPVTQVAD
DYADVRIYGDAARAAIAIAKDAGAKRPLLYVVPSATPKFGFASEVAALACGQELWQTLELREATGLTPAFEAIGLLSLSS
EKSHLLNALEAGRVLARDLCGTEPERMSAKAFAEYCLQAFKGSVIKTAVVEDRDILERDYPLLSAVARSSFAVGRHQPRV
VKLEYQPEGEITRTFLFAGKGVVYDTGGADLKVGGAMAGMSRDKGGASAVAGLFKTLSMLKPKGIRVIAELGLVRNSIGS
EAFVTDEIITSHAGVRVRIGNTDAEGRLVLADLLSHLRIKAVSAVQPELFSVATLTGHVVRCYGSYPAAVENAVASGAQC
AQTLALQASQWGEPFEVSTLRRDDFAKIREVSGAADILSSNNAPSSVTARGHQYPAAFLLKASGLFDHGINAAIPLAYTH
LDIAGSATEGDPLYGKPTASPLVGLYQYLINR

Sequences:

>Translated_512_residues
MFQVNFVDVQAENAIFDGEGWDAVVVVTPDLVSIGIDEISLLAEHGAKVDKRVGKSPTLLFAPGLAGGRLIIAPVTQVAD
DYADVRIYGDAARAAIAIAKDAGAKRPLLYVVPSATPKFGFASEVAALACGQELWQTLELREATGLTPAFEAIGLLSLSS
EKSHLLNALEAGRVLARDLCGTEPERMSAKAFAEYCLQAFKGSVIKTAVVEDRDILERDYPLLSAVARSSFAVGRHQPRV
VKLEYQPEGEITRTFLFAGKGVVYDTGGADLKVGGAMAGMSRDKGGASAVAGLFKTLSMLKPKGIRVIAELGLVRNSIGS
EAFVTDEIITSHAGVRVRIGNTDAEGRLVLADLLSHLRIKAVSAVQPELFSVATLTGHVVRCYGSYPAAVENAVASGAQC
AQTLALQASQWGEPFEVSTLRRDDFAKIREVSGAADILSSNNAPSSVTARGHQYPAAFLLKASGLFDHGINAAIPLAYTH
LDIAGSATEGDPLYGKPTASPLVGLYQYLINR
>Mature_512_residues
MFQVNFVDVQAENAIFDGEGWDAVVVVTPDLVSIGIDEISLLAEHGAKVDKRVGKSPTLLFAPGLAGGRLIIAPVTQVAD
DYADVRIYGDAARAAIAIAKDAGAKRPLLYVVPSATPKFGFASEVAALACGQELWQTLELREATGLTPAFEAIGLLSLSS
EKSHLLNALEAGRVLARDLCGTEPERMSAKAFAEYCLQAFKGSVIKTAVVEDRDILERDYPLLSAVARSSFAVGRHQPRV
VKLEYQPEGEITRTFLFAGKGVVYDTGGADLKVGGAMAGMSRDKGGASAVAGLFKTLSMLKPKGIRVIAELGLVRNSIGS
EAFVTDEIITSHAGVRVRIGNTDAEGRLVLADLLSHLRIKAVSAVQPELFSVATLTGHVVRCYGSYPAAVENAVASGAQC
AQTLALQASQWGEPFEVSTLRRDDFAKIREVSGAADILSSNNAPSSVTARGHQYPAAFLLKASGLFDHGINAAIPLAYTH
LDIAGSATEGDPLYGKPTASPLVGLYQYLINR

Specific function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI47155554, Length=364, Percent_Identity=30.2197802197802, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI41393561, Length=360, Percent_Identity=26.9444444444444, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1790710, Length=272, Percent_Identity=30.5147058823529, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082123, Length=264, Percent_Identity=32.1969696969697, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17565172, Length=449, Percent_Identity=42.5389755011136, Blast_Score=333, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17556903, Length=363, Percent_Identity=30.0275482093664, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24646701, Length=507, Percent_Identity=39.2504930966469, Blast_Score=335, Evalue=6e-92,
Organism=Drosophila melanogaster, GI24646703, Length=507, Percent_Identity=39.2504930966469, Blast_Score=335, Evalue=6e-92,
Organism=Drosophila melanogaster, GI21358201, Length=507, Percent_Identity=39.2504930966469, Blast_Score=335, Evalue=6e-92,
Organism=Drosophila melanogaster, GI221379063, Length=189, Percent_Identity=34.9206349206349, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI221379062, Length=189, Percent_Identity=34.9206349206349, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357381, Length=189, Percent_Identity=34.9206349206349, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011356
- InterPro:   IPR000819
- InterPro:   IPR023042
- InterPro:   IPR008283 [H]

Pfam domain/function: PF00883 Peptidase_M17; PF02789 Peptidase_M17_N [H]

EC number: =3.4.11.1; =3.4.11.10 [H]

Molecular weight: Translated: 54109; Mature: 54109

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQVNFVDVQAENAIFDGEGWDAVVVVTPDLVSIGIDEISLLAEHGAKVDKRVGKSPTLL
CEEEEEEEEEECCEEECCCCCCEEEEECCCHHHHCHHHHHHHHHCCCHHHHHCCCCCCEE
FAPGLAGGRLIIAPVTQVADDYADVRIYGDAARAAIAIAKDAGAKRPLLYVVPSATPKFG
EECCCCCCEEEEEEHHHHHCCCCCEEEECCHHHEEEEEECCCCCCCCEEEEECCCCCCCC
FASEVAALACGQELWQTLELREATGLTPAFEAIGLLSLSSEKSHLLNALEAGRVLARDLC
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHC
GTEPERMSAKAFAEYCLQAFKGSVIKTAVVEDRDILERDYPLLSAVARSSFAVGRHQPRV
CCCHHHHHHHHHHHHHHHHHCCCCEEEEHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCEE
VKLEYQPEGEITRTFLFAGKGVVYDTGGADLKVGGAMAGMSRDKGGASAVAGLFKTLSML
EEEEECCCCCEEEEEEEECCCEEEECCCCCEEECCHHCCCCCCCCCHHHHHHHHHHHHHH
KPKGIRVIAELGLVRNSIGSEAFVTDEIITSHAGVRVRIGNTDAEGRLVLADLLSHLRIK
CCCCHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHHHH
AVSAVQPELFSVATLTGHVVRCYGSYPAAVENAVASGAQCAQTLALQASQWGEPFEVSTL
HHHHCCCHHHHHHHHHCEEEEEECCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEECC
RRDDFAKIREVSGAADILSSNNAPSSVTARGHQYPAAFLLKASGLFDHGINAAIPLAYTH
CCHHHHHHHHHCCHHHHHCCCCCCCCEECCCCCCCCEEEEEECCCHHCCCCCEEEEEEEE
LDIAGSATEGDPLYGKPTASPLVGLYQYLINR
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MFQVNFVDVQAENAIFDGEGWDAVVVVTPDLVSIGIDEISLLAEHGAKVDKRVGKSPTLL
CEEEEEEEEEECCEEECCCCCCEEEEECCCHHHHCHHHHHHHHHCCCHHHHHCCCCCCEE
FAPGLAGGRLIIAPVTQVADDYADVRIYGDAARAAIAIAKDAGAKRPLLYVVPSATPKFG
EECCCCCCEEEEEEHHHHHCCCCCEEEECCHHHEEEEEECCCCCCCCEEEEECCCCCCCC
FASEVAALACGQELWQTLELREATGLTPAFEAIGLLSLSSEKSHLLNALEAGRVLARDLC
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHC
GTEPERMSAKAFAEYCLQAFKGSVIKTAVVEDRDILERDYPLLSAVARSSFAVGRHQPRV
CCCHHHHHHHHHHHHHHHHHCCCCEEEEHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCEE
VKLEYQPEGEITRTFLFAGKGVVYDTGGADLKVGGAMAGMSRDKGGASAVAGLFKTLSML
EEEEECCCCCEEEEEEEECCCEEEECCCCCEEECCHHCCCCCCCCCHHHHHHHHHHHHHH
KPKGIRVIAELGLVRNSIGSEAFVTDEIITSHAGVRVRIGNTDAEGRLVLADLLSHLRIK
CCCCHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHHHH
AVSAVQPELFSVATLTGHVVRCYGSYPAAVENAVASGAQCAQTLALQASQWGEPFEVSTL
HHHHCCCHHHHHHHHHCEEEEEECCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEECC
RRDDFAKIREVSGAADILSSNNAPSSVTARGHQYPAAFLLKASGLFDHGINAAIPLAYTH
CCHHHHHHHHHCCHHHHHCCCCCCCCEECCCCCCCCEEEEEECCCHHCCCCCEEEEEEEE
LDIAGSATEGDPLYGKPTASPLVGLYQYLINR
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]