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The map label for this gene is rsuA [H]

Identifier: 117919212

GI number: 117919212

Start: 882233

End: 882940

Strand: Direct

Name: rsuA [H]

Synonym: Shewana3_0760

Alternate gene names: 117919212

Gene position: 882233-882940 (Clockwise)

Preceding gene: 117919207

Following gene: 117919216

Centisome position: 17.74

GC content: 48.45

Gene sequence:

>708_bases
TTGAAGTTGAGTCGTCCCGTGCGTTTAGACAAATTTATCTGTGAAAGTACCTCGCTTACCCGTTCTTTAGCGAAAAAAGC
CCTGCACCGTGGTGACATCACCTGTGATGGGGCTGTGATTAAAGATGCTGGTTTTAAAGTGCTCGAGGGGATGCAAGTAT
GTCTCGAAGGTGAGTTTATCTCCCTCGTTGGTGAGCGCTACCTGATGCTAAATAAGCCTGTGGATACGATTTGCTCGACC
ATAGATGAAGTCTACCCTTCGGTATTGAGCTTGCTGGATATTGAAAAGCCCGAAACCTTACACATTGCCGGCCGTTTAGA
TGCCGATACTACTGGCCTTGTGCTGATCACGAGCGATGGTCAATGGTCGCATCGGATCACCTCGCCTAAAAAAGACTGTG
GCAAGCGTTACTTAGTGACCTTAGCCGAGCCTGTGGATGAGAGTCTGATTGGTGTCTTTGCTGCTGGAGTGGAGCTGAGA
AACGAAGACTGCCTGACAAAACCCGCCTTGCTTGAAATCATCGAGCCGCAGCTGGTGCGTTTAACCATTACCGAAGGTAA
GTATCACCAAGTGAAGCGGATGTTTGCCGCCGTAGGTAATCATGTGGTTGGCTTACACCGTGAAAGCATTGGCAAAATTG
AATTAGACGCAGAACTTGCCCTAGGTGAATGGCGTTATCTGACGAGCGAAGAAATCGCCTCGATTTAA

Upstream 100 bases:

>100_bases
TAGGTTTGTCTGGCGTAACTAAGGGGGCATTTGGGCAATTTGATTGGCTAGGCTTTGCCCTTTCGTTCGTTGGGCCTCAT
AATACGGCGGTTTTCATTTA

Downstream 100 bases:

>100_bases
CACCGTTTGGCTAAAAAAATGCCGCTCCTGATGAGCGGCATTTTTATTGCCTTTTTATCGCTGATTCGTGTTTTCTGATA
AGGCTATTGTTTTTTCAGGA

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKLSRPVRLDKFICESTSLTRSLAKKALHRGDITCDGAVIKDAGFKVLEGMQVCLEGEFISLVGERYLMLNKPVDTICST
IDEVYPSVLSLLDIEKPETLHIAGRLDADTTGLVLITSDGQWSHRITSPKKDCGKRYLVTLAEPVDESLIGVFAAGVELR
NEDCLTKPALLEIIEPQLVRLTITEGKYHQVKRMFAAVGNHVVGLHRESIGKIELDAELALGEWRYLTSEEIASI

Sequences:

>Translated_235_residues
MKLSRPVRLDKFICESTSLTRSLAKKALHRGDITCDGAVIKDAGFKVLEGMQVCLEGEFISLVGERYLMLNKPVDTICST
IDEVYPSVLSLLDIEKPETLHIAGRLDADTTGLVLITSDGQWSHRITSPKKDCGKRYLVTLAEPVDESLIGVFAAGVELR
NEDCLTKPALLEIIEPQLVRLTITEGKYHQVKRMFAAVGNHVVGLHRESIGKIELDAELALGEWRYLTSEEIASI
>Mature_235_residues
MKLSRPVRLDKFICESTSLTRSLAKKALHRGDITCDGAVIKDAGFKVLEGMQVCLEGEFISLVGERYLMLNKPVDTICST
IDEVYPSVLSLLDIEKPETLHIAGRLDADTTGLVLITSDGQWSHRITSPKKDCGKRYLVTLAEPVDESLIGVFAAGVELR
NEDCLTKPALLEIIEPQLVRLTITEGKYHQVKRMFAAVGNHVVGLHRESIGKIELDAELALGEWRYLTSEEIASI

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=56.5217391304348, Blast_Score=257, Evalue=4e-70,
Organism=Escherichia coli, GI1790453, Length=231, Percent_Identity=31.1688311688312, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1787524, Length=231, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI87081838, Length=180, Percent_Identity=32.2222222222222, Blast_Score=77, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26004; Mature: 26004

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSRPVRLDKFICESTSLTRSLAKKALHRGDITCDGAVIKDAGFKVLEGMQVCLEGEFI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCEEECCCCHHHHHHHHHHHCCHHH
SLVGERYLMLNKPVDTICSTIDEVYPSVLSLLDIEKPETLHIAGRLDADTTGLVLITSDG
HHHCCHHEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEECCC
QWSHRITSPKKDCGKRYLVTLAEPVDESLIGVFAAGVELRNEDCLTKPALLEIIEPQLVR
CCCCCCCCCHHHHCCEEEEEECCHHHHHHHHHHHHCCEECCCCCCCCHHHHHHCCCEEEE
LTITEGKYHQVKRMFAAVGNHVVGLHRESIGKIELDAELALGEWRYLTSEEIASI
EEECCCHHHHHHHHHHHHCCCEEEEECCCCCCEEECCEEECCCCEECCHHHHHCC
>Mature Secondary Structure
MKLSRPVRLDKFICESTSLTRSLAKKALHRGDITCDGAVIKDAGFKVLEGMQVCLEGEFI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCEEECCCCHHHHHHHHHHHCCHHH
SLVGERYLMLNKPVDTICSTIDEVYPSVLSLLDIEKPETLHIAGRLDADTTGLVLITSDG
HHHCCHHEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEECCC
QWSHRITSPKKDCGKRYLVTLAEPVDESLIGVFAAGVELRNEDCLTKPALLEIIEPQLVR
CCCCCCCCCHHHHCCEEEEEECCHHHHHHHHHHHHCCEECCCCCCCCHHHHHHCCCEEEE
LTITEGKYHQVKRMFAAVGNHVVGLHRESIGKIELDAELALGEWRYLTSEEIASI
EEECCCHHHHHHHHHHHHCCCEEEEECCCCCCEEECCEEECCCCEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]