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The map label for this gene is mlaE [H]

Identifier: 117919131

GI number: 117919131

Start: 798274

End: 799059

Strand: Direct

Name: mlaE [H]

Synonym: Shewana3_0679

Alternate gene names: 117919131

Gene position: 798274-799059 (Clockwise)

Preceding gene: 117919130

Following gene: 117919132

Centisome position: 16.05

GC content: 53.82

Gene sequence:

>786_bases
GTGAAGTTATTCGACAGTATTGCCGACCTCGGCCGCAGCGCTATCGATTTAGTGCTCGGTTTTGGCCGTGCGGGCCTGAT
GCTCTGGGGCGCGATTTTTCGCTGGCCTCGGGTGCGTAAAGGTTTTCCATTATTAGTGCGCCAAATCTATGTGGTCGGCG
TACAATCTATGGTCATTATTGTGGTCTCGGGCCTGTTTATCGGCATGGTGTTGGCGCTGCAGGGCTACAACATCTTAGTT
GGTTTCGGTACTGAGGAGAGCTTAGGCCCCATGGTGGCCTTAAGCTTACTACGTGAACTCGGTCCTGTGGTCACAGCATT
ATTATTCGCTGGCCGCGCTGGTTCGGCGCTGACCGCCGAAATCGGTCTGATGAAGAGTACAGAGCAGTTATCCAGCTTAG
AGATGATGGCGATTGACCCACTTAGGCAAATCATTGCGCCACGCTTTTGGGCGGGGGTGATCAGCATGCCGTTATTGGCG
CTGATGTTTACTGCGGTAGGCATTTATGGCGGCCATTTAGTGGGTGTGGAATGGAAAGGCATCGATGGCGGGGCCTTCTG
GTCGATTCTGCAAGCCTCGGTCGAATGGCGCCAAGACATAGTGAACTGCCTGATTAAGAGCGGCGTGTTTGCCGTGGTGG
TGACTTGGATTGCCTTATACCGTGGTTATCAAGTGGTGCCGAATCCGGAAGGGATCAGCCGTGCAACCACCTCAACCGTG
GTGCAATCGAGCCTCGCCGTACTGGCATTGGACTTTTTACTAACAGCCCTGATGTTTGGCCGCTAA

Upstream 100 bases:

>100_bases
AGCTCAAACAGTTTATTCAAGGCGCGCCCGACGGCCCTGTGCCTTTCCACTATCCGGCGAAGGATTATCAGCAAGAATTA
ATGGCTAAAGGAGCTGGCAA

Downstream 100 bases:

>100_bases
CGGATTGCAGCGCCAAGGATACAGATAGACGGATTAACAGAGTGGTAACTGAGCATGTTGACACGAAAAATTGAATTATT
AGTGGGTATGTTTCTCTTAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKLFDSIADLGRSAIDLVLGFGRAGLMLWGAIFRWPRVRKGFPLLVRQIYVVGVQSMVIIVVSGLFIGMVLALQGYNILV
GFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLA
LMFTAVGIYGGHLVGVEWKGIDGGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVVPNPEGISRATTSTV
VQSSLAVLALDFLLTALMFGR

Sequences:

>Translated_261_residues
MKLFDSIADLGRSAIDLVLGFGRAGLMLWGAIFRWPRVRKGFPLLVRQIYVVGVQSMVIIVVSGLFIGMVLALQGYNILV
GFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLA
LMFTAVGIYGGHLVGVEWKGIDGGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVVPNPEGISRATTSTV
VQSSLAVLALDFLLTALMFGR
>Mature_261_residues
MKLFDSIADLGRSAIDLVLGFGRAGLMLWGAIFRWPRVRKGFPLLVRQIYVVGVQSMVIIVVSGLFIGMVLALQGYNILV
GFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLA
LMFTAVGIYGGHLVGVEWKGIDGGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVVPNPEGISRATTSTV
VQSSLAVLALDFLLTALMFGR

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=259, Percent_Identity=68.7258687258687, Blast_Score=353, Evalue=7e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 28163; Mature: 28163

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFDSIADLGRSAIDLVLGFGRAGLMLWGAIFRWPRVRKGFPLLVRQIYVVGVQSMVII
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
VVSGLFIGMVLALQGYNILVGFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAE
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLALMFTAVGIYGGHLVGVEWKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEC
IDGGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVVPNPEGISRATTSTV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHH
VQSSLAVLALDFLLTALMFGR
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLFDSIADLGRSAIDLVLGFGRAGLMLWGAIFRWPRVRKGFPLLVRQIYVVGVQSMVII
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
VVSGLFIGMVLALQGYNILVGFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAE
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLALMFTAVGIYGGHLVGVEWKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEC
IDGGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVVPNPEGISRATTSTV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHH
VQSSLAVLALDFLLTALMFGR
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]