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The map label for this gene is fixJ [H]

Identifier: 117919089

GI number: 117919089

Start: 751123

End: 751734

Strand: Direct

Name: fixJ [H]

Synonym: Shewana3_0637

Alternate gene names: 117919089

Gene position: 751123-751734 (Clockwise)

Preceding gene: 117919088

Following gene: 117919090

Centisome position: 15.11

GC content: 53.1

Gene sequence:

>612_bases
ATGAGCCACGCATTGCCCGTGTATTTAATCGATGATGATGAGTCGGTGCGCCGCTCGCTGCGGTTTATGCTCGAAAGTTA
TGGCTTGAATATCCGCGACTTTGATAGCGCCGAGGCGTTTTTTGCCGCCATCGACTTAAGCCAGCCGGGTTGCGCCTTAG
TCGATGTGCGTATGCCGGGCTTAAGTGGGCCGCAATTACATGCGCAATTAGTGCAGCACAATAGTCCGTTGGCGGTGATT
TATTTGACTGGCCATGGCGATGTGCCCATGGCGGTGGAGGCGCTAAAACTCGGGGCGGTGGATTTTTTCCAAAAGCCCGC
CGATGGTGCCAAGTTGGCCGACGCAGTCGTTAAAGCGCTAGAGCATGCCAAGGCGCATTATCAAGATAATCAATATCTTC
AGACTTATCAGACTCTCACACCGCGGGAGCGGGAAATCCTTACCCTTATCGCCAAAGGCTATAAGAATCAGGAGATCGCC
GATACGCTGTGCATCGCCATGCGTACGGTCGAAATTCACCGTGCGAACTTAATGAAAGGCATGCAGGTCGGCTCGCTCGC
CGAGATGATATTAATCTACGGCCGGATTGTCGATCGCCTTGCCCCCACATAA

Upstream 100 bases:

>100_bases
TCGCCGAGAGTCATGGCGGCCGCTTTAGCCTAGAAACCGCTTTAAGTGGCGGCTGCCAAGCGCAGGTATCCTTACCCCGT
AAGTTTGATGGAGCACCAGC

Downstream 100 bases:

>100_bases
AACCGCCGTTGTTCGCGCGAATTGGCGTATCCTTGGCCGTTTGATGTGAGCTAACGCATTCAGCCCTAGGTGCTTAATTG
CCAAGGCTCGACGATTGCCT

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MSHALPVYLIDDDESVRRSLRFMLESYGLNIRDFDSAEAFFAAIDLSQPGCALVDVRMPGLSGPQLHAQLVQHNSPLAVI
YLTGHGDVPMAVEALKLGAVDFFQKPADGAKLADAVVKALEHAKAHYQDNQYLQTYQTLTPREREILTLIAKGYKNQEIA
DTLCIAMRTVEIHRANLMKGMQVGSLAEMILIYGRIVDRLAPT

Sequences:

>Translated_203_residues
MSHALPVYLIDDDESVRRSLRFMLESYGLNIRDFDSAEAFFAAIDLSQPGCALVDVRMPGLSGPQLHAQLVQHNSPLAVI
YLTGHGDVPMAVEALKLGAVDFFQKPADGAKLADAVVKALEHAKAHYQDNQYLQTYQTLTPREREILTLIAKGYKNQEIA
DTLCIAMRTVEIHRANLMKGMQVGSLAEMILIYGRIVDRLAPT
>Mature_202_residues
SHALPVYLIDDDESVRRSLRFMLESYGLNIRDFDSAEAFFAAIDLSQPGCALVDVRMPGLSGPQLHAQLVQHNSPLAVIY
LTGHGDVPMAVEALKLGAVDFFQKPADGAKLADAVVKALEHAKAHYQDNQYLQTYQTLTPREREILTLIAKGYKNQEIAD
TLCIAMRTVEIHRANLMKGMQVGSLAEMILIYGRIVDRLAPT

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=135, Percent_Identity=35.5555555555556, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1788222, Length=194, Percent_Identity=23.1958762886598, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22480; Mature: 22349

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHALPVYLIDDDESVRRSLRFMLESYGLNIRDFDSAEAFFAAIDLSQPGCALVDVRMPG
CCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEEECCCCCCEEEEEECCC
LSGPQLHAQLVQHNSPLAVIYLTGHGDVPMAVEALKLGAVDFFQKPADGAKLADAVVKAL
CCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
EHAKAHYQDNQYLQTYQTLTPREREILTLIAKGYKNQEIADTLCIAMRTVEIHRANLMKG
HHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
MQVGSLAEMILIYGRIVDRLAPT
CCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SHALPVYLIDDDESVRRSLRFMLESYGLNIRDFDSAEAFFAAIDLSQPGCALVDVRMPG
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEEECCCCCCEEEEEECCC
LSGPQLHAQLVQHNSPLAVIYLTGHGDVPMAVEALKLGAVDFFQKPADGAKLADAVVKAL
CCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
EHAKAHYQDNQYLQTYQTLTPREREILTLIAKGYKNQEIADTLCIAMRTVEIHRANLMKG
HHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
MQVGSLAEMILIYGRIVDRLAPT
CCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]