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The map label for this gene is fixL [H]

Identifier: 117919088

GI number: 117919088

Start: 749138

End: 751126

Strand: Direct

Name: fixL [H]

Synonym: Shewana3_0636

Alternate gene names: 117919088

Gene position: 749138-751126 (Clockwise)

Preceding gene: 117919083

Following gene: 117919089

Centisome position: 15.07

GC content: 50.43

Gene sequence:

>1989_bases
ATGAGCGTTGGCTTTTGTGCTGCGCGATGGCCTAGTAATACGCTTATCATCTTATTGATATTATTAGTGTGTAGCCCTGC
TGCCATGTCAAGCACGGGCGTGAATCCTGCCGCAGTTAACGTGCTTGGGGGGCAAGAGTCCACCACAGAATCATCAACAT
CCCTTACCTTAGCCGCCCTCGATGCAAATAACGCTTTGGTTAGCGACAGTCTTTCGACTTTGTCTGCAGAGGCGGGTTAT
CAAGTGGTCGATGTAGGCGTATTGGCCATTCGGGGCTATAAGAGCACAGTCAATCGTTGGCAACCTTTGATGGGCTGGCT
AGAGTCGCAAATACCTAATAGTTACTTTAGATTGCATCCTTTAAGCATTGAAGAATTAGCCAAAGGCGTTGAAACCGAAG
CGTTGGACTTTGTTATCACTAACCCTGGGCAGTCGGTGATGTTGGCGCGGCAATTTTCCCTGTCTTGGCTCGCTACCCTT
CGAAGCCCAATCAATAATGGCGCCGCGATGCAAGTGGGGTCTGCGCTGGTGGTCAGAGCCGATTCACCCTATCAGACCTT
ACAGGATCTTAAGGGCAAGCGCCTTGGAATTGTCTCCAAAAATGCCTTTGGTGGTTATCTTACTTTAGTTTATGAGGCAC
AGCTTAAGGGCATAGAATTACCTGGTTTTGTGGGGGAGATTATTCCCTTAGGTTTTCCACTCGATAACCTTATTTATCAG
CTCGGCGCGACAGATAAGCCAAGCGATAAATCCCCAAGTTTAGATGCAGCGGTCGTGCCTGTATGTCAGCTTGAGCAAAT
GCAGGCTGAAGGTTTGATCAAGCTGCAAAATTATCGGGTGCTCGATAATCAGGCGCCAGCGGGGTTTCATTGCCAAGTCT
CGAGTCGGTTATACCCCAACTGGTCTATGGCTAAAACCAACCGTGCGACTCAATCGCTCGCGAAACAGGTGACTCAAGCC
CTGTTTGCCCTACCCGAAGACAGTCCCGCTGCTAAAGCCGCCGAATCTGCGGGTTGGACCACGGCTGTGAGTCAATTGGC
GATAGATCAACTCCTTAAAGATCTCGATATGCATCCCCTGCAAACGCCTTGGTGGCAGCGGGCGTGGCAATGGGTAAAAC
TGCATCAACATTGGGCATGGTTTGTGTTAACCATATTGGTATTGCTCAATGTGTACCATTTCTGGCTCGAATATCGTTTT
AGTCGCCGCGGCCATGAGTTGATAAACACCCAGAGGCAACTCAGTGAAAACCGCGTTCTATTGGAGCACGCCCAGCGGAT
TGCGATTGCGGGGGAGTTAGGGGCGAGCCTCTCCCATGAACTCAATCAACCTTTGGCGGCGATTGGCCATTATTGCCATG
GCGCCGAGGTACGTTTACAACGAGGGACGAGTCCTGAAGAATTACAATCCGTGCTGACTTTAATCCAGCAAGAAGTCAGC
CGCGCCGACAGTATTATCAGCCGTTTACGTAATTTATTGAAAAAGCGGCCTGTGTCTAAGCAGCTTCTGTATTTACATCA
GTTAGTGAACGATACTGCGCCGCTGCTCGCCTATGAACTGGAGCAGCACCATATCCAGCTTTCGACCAATGTGAGCGGCG
AAGCCTATCAATTGCCTTTAGATGAAGTGGGCATGCAGCAACTATTGCTCAACCTTTTGAAAAACGCCGCCGATGCCTGT
GTGCAACGCCAGCAGAGCGAGGTCGATGCGTCGAAACCCTATCAGCCCACGATTGATATTGACCTGCGTTACCAAGAACA
CAAGCTACTGCTGACGGTCACAGACAATGGCACAGGTTTAACTGAGGATGCCAATCTGCTGATGCAGGCCTTTTATTCGA
CTAAGTCAGAGGGCTTAGGGCTAGGGCTGGTGATTTGCCGTGATATCGCCGAGAGTCATGGCGGCCGCTTTAGCCTAGAA
ACCGCTTTAAGTGGCGGCTGCCAAGCGCAGGTATCCTTACCCCGTAAGTTTGATGGAGCACCAGCATGA

Upstream 100 bases:

>100_bases
CAAATATTCGCTACAATAGCGCATTCTGCTGTGATTGTTTTTTGTAAGTTATGGGGACGAAGTATTGTACAGGACGTTAT
TTTGCATACTCCAAACAGCG

Downstream 100 bases:

>100_bases
GCCACGCATTGCCCGTGTATTTAATCGATGATGATGAGTCGGTGCGCCGCTCGCTGCGGTTTATGCTCGAAAGTTATGGC
TTGAATATCCGCGACTTTGA

Product: ATPase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MSVGFCAARWPSNTLIILLILLVCSPAAMSSTGVNPAAVNVLGGQESTTESSTSLTLAALDANNALVSDSLSTLSAEAGY
QVVDVGVLAIRGYKSTVNRWQPLMGWLESQIPNSYFRLHPLSIEELAKGVETEALDFVITNPGQSVMLARQFSLSWLATL
RSPINNGAAMQVGSALVVRADSPYQTLQDLKGKRLGIVSKNAFGGYLTLVYEAQLKGIELPGFVGEIIPLGFPLDNLIYQ
LGATDKPSDKSPSLDAAVVPVCQLEQMQAEGLIKLQNYRVLDNQAPAGFHCQVSSRLYPNWSMAKTNRATQSLAKQVTQA
LFALPEDSPAAKAAESAGWTTAVSQLAIDQLLKDLDMHPLQTPWWQRAWQWVKLHQHWAWFVLTILVLLNVYHFWLEYRF
SRRGHELINTQRQLSENRVLLEHAQRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQRGTSPEELQSVLTLIQQEVS
RADSIISRLRNLLKKRPVSKQLLYLHQLVNDTAPLLAYELEQHHIQLSTNVSGEAYQLPLDEVGMQQLLLNLLKNAADAC
VQRQQSEVDASKPYQPTIDIDLRYQEHKLLLTVTDNGTGLTEDANLLMQAFYSTKSEGLGLGLVICRDIAESHGGRFSLE
TALSGGCQAQVSLPRKFDGAPA

Sequences:

>Translated_662_residues
MSVGFCAARWPSNTLIILLILLVCSPAAMSSTGVNPAAVNVLGGQESTTESSTSLTLAALDANNALVSDSLSTLSAEAGY
QVVDVGVLAIRGYKSTVNRWQPLMGWLESQIPNSYFRLHPLSIEELAKGVETEALDFVITNPGQSVMLARQFSLSWLATL
RSPINNGAAMQVGSALVVRADSPYQTLQDLKGKRLGIVSKNAFGGYLTLVYEAQLKGIELPGFVGEIIPLGFPLDNLIYQ
LGATDKPSDKSPSLDAAVVPVCQLEQMQAEGLIKLQNYRVLDNQAPAGFHCQVSSRLYPNWSMAKTNRATQSLAKQVTQA
LFALPEDSPAAKAAESAGWTTAVSQLAIDQLLKDLDMHPLQTPWWQRAWQWVKLHQHWAWFVLTILVLLNVYHFWLEYRF
SRRGHELINTQRQLSENRVLLEHAQRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQRGTSPEELQSVLTLIQQEVS
RADSIISRLRNLLKKRPVSKQLLYLHQLVNDTAPLLAYELEQHHIQLSTNVSGEAYQLPLDEVGMQQLLLNLLKNAADAC
VQRQQSEVDASKPYQPTIDIDLRYQEHKLLLTVTDNGTGLTEDANLLMQAFYSTKSEGLGLGLVICRDIAESHGGRFSLE
TALSGGCQAQVSLPRKFDGAPA
>Mature_661_residues
SVGFCAARWPSNTLIILLILLVCSPAAMSSTGVNPAAVNVLGGQESTTESSTSLTLAALDANNALVSDSLSTLSAEAGYQ
VVDVGVLAIRGYKSTVNRWQPLMGWLESQIPNSYFRLHPLSIEELAKGVETEALDFVITNPGQSVMLARQFSLSWLATLR
SPINNGAAMQVGSALVVRADSPYQTLQDLKGKRLGIVSKNAFGGYLTLVYEAQLKGIELPGFVGEIIPLGFPLDNLIYQL
GATDKPSDKSPSLDAAVVPVCQLEQMQAEGLIKLQNYRVLDNQAPAGFHCQVSSRLYPNWSMAKTNRATQSLAKQVTQAL
FALPEDSPAAKAAESAGWTTAVSQLAIDQLLKDLDMHPLQTPWWQRAWQWVKLHQHWAWFVLTILVLLNVYHFWLEYRFS
RRGHELINTQRQLSENRVLLEHAQRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQRGTSPEELQSVLTLIQQEVSR
ADSIISRLRNLLKKRPVSKQLLYLHQLVNDTAPLLAYELEQHHIQLSTNVSGEAYQLPLDEVGMQQLLLNLLKNAADACV
QRQQSEVDASKPYQPTIDIDLRYQEHKLLLTVTDNGTGLTEDANLLMQAFYSTKSEGLGLGLVICRDIAESHGGRFSLET
ALSGGCQAQVSLPRKFDGAPA

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=275, Percent_Identity=25.8181818181818, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788549, Length=248, Percent_Identity=26.2096774193548, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 72724; Mature: 72593

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVGFCAARWPSNTLIILLILLVCSPAAMSSTGVNPAAVNVLGGQESTTESSTSLTLAAL
CCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHEEEEECCCCCCCCCCCEEEEEEE
DANNALVSDSLSTLSAEAGYQVVDVGVLAIRGYKSTVNRWQPLMGWLESQIPNSYFRLHP
CCCCCCHHHHHHHHHHHCCCEEEEEEEHEEECCHHHHHHHHHHHHHHHHHCCCCEEEECC
LSIEELAKGVETEALDFVITNPGQSVMLARQFSLSWLATLRSPINNGAAMQVGSALVVRA
CCHHHHHCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEC
DSPYQTLQDLKGKRLGIVSKNAFGGYLTLVYEAQLKGIELPGFVGEIIPLGFPLDNLIYQ
CCHHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCCHHHHHHH
LGATDKPSDKSPSLDAAVVPVCQLEQMQAEGLIKLQNYRVLDNQAPAGFHCQVSSRLYPN
HCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCEEEECEEEECCCCCCCEEEEECCCCCCC
WSMAKTNRATQSLAKQVTQALFALPEDSPAAKAAESAGWTTAVSQLAIDQLLKDLDMHPL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
QTPWWQRAWQWVKLHQHWAWFVLTILVLLNVYHFWLEYRFSRRGHELINTQRQLSENRVL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHH
LEHAQRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQRGTSPEELQSVLTLIQQEVS
HHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHH
RADSIISRLRNLLKKRPVSKQLLYLHQLVNDTAPLLAYELEQHHIQLSTNVSGEAYQLPL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCEEEEEECCCCCEEECCH
DEVGMQQLLLNLLKNAADACVQRQQSEVDASKPYQPTIDIDLRYQEHKLLLTVTDNGTGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCEEEEEEECCCCCC
TEDANLLMQAFYSTKSEGLGLGLVICRDIAESHGGRFSLETALSGGCQAQVSLPRKFDGA
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHCCCCEEEEECCCCCCCC
PA
CC
>Mature Secondary Structure 
SVGFCAARWPSNTLIILLILLVCSPAAMSSTGVNPAAVNVLGGQESTTESSTSLTLAAL
CCCCEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHEEEEECCCCCCCCCCCEEEEEEE
DANNALVSDSLSTLSAEAGYQVVDVGVLAIRGYKSTVNRWQPLMGWLESQIPNSYFRLHP
CCCCCCHHHHHHHHHHHCCCEEEEEEEHEEECCHHHHHHHHHHHHHHHHHCCCCEEEECC
LSIEELAKGVETEALDFVITNPGQSVMLARQFSLSWLATLRSPINNGAAMQVGSALVVRA
CCHHHHHCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEC
DSPYQTLQDLKGKRLGIVSKNAFGGYLTLVYEAQLKGIELPGFVGEIIPLGFPLDNLIYQ
CCHHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCCHHHHHHH
LGATDKPSDKSPSLDAAVVPVCQLEQMQAEGLIKLQNYRVLDNQAPAGFHCQVSSRLYPN
HCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCEEEECEEEECCCCCCCEEEEECCCCCCC
WSMAKTNRATQSLAKQVTQALFALPEDSPAAKAAESAGWTTAVSQLAIDQLLKDLDMHPL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
QTPWWQRAWQWVKLHQHWAWFVLTILVLLNVYHFWLEYRFSRRGHELINTQRQLSENRVL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHH
LEHAQRIAIAGELGASLSHELNQPLAAIGHYCHGAEVRLQRGTSPEELQSVLTLIQQEVS
HHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHH
RADSIISRLRNLLKKRPVSKQLLYLHQLVNDTAPLLAYELEQHHIQLSTNVSGEAYQLPL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCEEEEEECCCCCEEECCH
DEVGMQQLLLNLLKNAADACVQRQQSEVDASKPYQPTIDIDLRYQEHKLLLTVTDNGTGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCEEEEEEECCCCCC
TEDANLLMQAFYSTKSEGLGLGLVICRDIAESHGGRFSLETALSGGCQAQVSLPRKFDGA
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHCCCCEEEEECCCCCCCC
PA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]