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The map label for this gene is silS [H]

Identifier: 117676234

GI number: 117676234

Start: 178084

End: 179559

Strand: Reverse

Name: silS [H]

Synonym: Shewana3_4295

Alternate gene names: 117676234

Gene position: 179559-178084 (Counterclockwise)

Preceding gene: 117676235

Following gene: 117676231

Centisome position: 64.37

GC content: 49.93

Gene sequence:

>1476_bases
ATGCATAGCAAACCTTCCAGGCACCCTTTCTCACTCTCCCTGCGGCTGACCTTTTTTATCAGCCTGTCCACGATACTGGC
TTTTATCGCCTTCACCTGGTTTATGCTGCATTCTGTTGAAAATCATTTTGCCGAGCAGGATGTCAGCGATCTTCAACAAA
TCAGCACCACACTGAACCGTATACTCCAGTCCCCGGAAGATCCAGATGAAAAAAAAGTAAGCAAAATAAAGGAATCGATT
GCCAGCTACCGCAACGTTGCCCTTTTGCTCCTCAATCCCAGGGGTGAGGTGCTCTTTAGTTCAGCGCAGGGGGCAGCACT
ACGCCCGGCCGTGAATTCAGCAGATTTTAGCGAGCACAGCCACGCACGGGATGTCTTTCTCTGGACGGTGGAGGATCCTG
CGAGACCGATGGATACCGGGTCTGAAATGAAGATGGAAACATACCGGATTATCGCCTCCTCTGGCCAGGCGATATTTCAG
GGCAAACAGCAGAACTATGTCATGCTGACTGGCCTCTCCATTAATTTCCATCTCCATTACCTCGATGCGCTGAAAAAAAA
TCTGATTGCGATTGCCGTCGTGATAAGCCTGCTGATTGTTCTGATCATTCGCATCGCTGTCCGTCAGGGGCACCTGCCCC
TTCGTAATGTCAGCAATGCCATTAAAAGCATCACCTCCGAGAATCTTGATACGCGGCTGGAACCGACACGCGTTCCCATT
GAGCTGGAACAGCTGGTTATCTCGTTCAATCATATGATTGGAAAGATTGAGGATGTCTTTACCCGCCAGGCCAATTTCTC
TGCCGATATCGCGCACGAGATCAGAACGCCCATCACCAATCTGGTGACGCAGACTGAAATCGCACTGAGTCAGGATCGAA
CCCAGAAGGAACTTGAGGATGTCCTCTACTCCAGTCTTGAAGAGTATAACCGGATGACCAAAATGGTCAGCGACATGCTG
TTCCTGGCGCAGGCGGACAATAATCAGCTTATACCTGACAGGGTCCGGTTTGACCTCAGAGCAGAAGTCATGAAAGTCTT
CGAGTTTTTCGAAGCCTGGGCCGAAGAACGCAATATCACGCTCAAATTTAACGGGATGCCCTGCCTGGTTGAGGGAGATC
CACAAATGTTCAGAAGAGCGATCAATAATCTGTTATCCAATGCCCTGCGTTATACCCCGGAGGGACAGGCAATCACCGTC
TCAATAAGAGAGCAAGAGAGCTTTTTTGACCTGGTGATTGAAAATCCGGGGAAACCAATCCCTGAAGAGCATTTATCAAG
GCTTTTTGACCGTTTTTATCGGGTGGATCCGTCCAGACAACGAAAAGGAGAAGGCAGCGGCATCGGCCTTGCAATTGTGA
AGTCAATCGTGGAAGCACATCACGGAAGAGTGCAGGTGGAATCGGACGTACGCTCAACTCGTTTTATCTTATCCGTGCCC
AGACTGGAGCAAAGGATTCCGGAGCCCCAACACTGA

Upstream 100 bases:

>100_bases
GATGTCGCCGTAAAGCGACTCCGCGCTAAAATAGACAACGATTACGAGACAAAGCTGATCCAGACAGTCCGGGGCGTGGG
CTATATGCTGGAGGTCCCGG

Downstream 100 bases:

>100_bases
GAATAAAGATTTAAATGACAAAGATGTCATTAGCCTGTCATGCAGCAAACAGAAGCCATTCGATACTTATCAGGAAGGCT
GGATTGCTTCATCAATACCC

Product: sensor kinase CusS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MHSKPSRHPFSLSLRLTFFISLSTILAFIAFTWFMLHSVENHFAEQDVSDLQQISTTLNRILQSPEDPDEKKVSKIKESI
ASYRNVALLLLNPRGEVLFSSAQGAALRPAVNSADFSEHSHARDVFLWTVEDPARPMDTGSEMKMETYRIIASSGQAIFQ
GKQQNYVMLTGLSINFHLHYLDALKKNLIAIAVVISLLIVLIIRIAVRQGHLPLRNVSNAIKSITSENLDTRLEPTRVPI
ELEQLVISFNHMIGKIEDVFTRQANFSADIAHEIRTPITNLVTQTEIALSQDRTQKELEDVLYSSLEEYNRMTKMVSDML
FLAQADNNQLIPDRVRFDLRAEVMKVFEFFEAWAEERNITLKFNGMPCLVEGDPQMFRRAINNLLSNALRYTPEGQAITV
SIREQESFFDLVIENPGKPIPEEHLSRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVEAHHGRVQVESDVRSTRFILSVP
RLEQRIPEPQH

Sequences:

>Translated_491_residues
MHSKPSRHPFSLSLRLTFFISLSTILAFIAFTWFMLHSVENHFAEQDVSDLQQISTTLNRILQSPEDPDEKKVSKIKESI
ASYRNVALLLLNPRGEVLFSSAQGAALRPAVNSADFSEHSHARDVFLWTVEDPARPMDTGSEMKMETYRIIASSGQAIFQ
GKQQNYVMLTGLSINFHLHYLDALKKNLIAIAVVISLLIVLIIRIAVRQGHLPLRNVSNAIKSITSENLDTRLEPTRVPI
ELEQLVISFNHMIGKIEDVFTRQANFSADIAHEIRTPITNLVTQTEIALSQDRTQKELEDVLYSSLEEYNRMTKMVSDML
FLAQADNNQLIPDRVRFDLRAEVMKVFEFFEAWAEERNITLKFNGMPCLVEGDPQMFRRAINNLLSNALRYTPEGQAITV
SIREQESFFDLVIENPGKPIPEEHLSRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVEAHHGRVQVESDVRSTRFILSVP
RLEQRIPEPQH
>Mature_491_residues
MHSKPSRHPFSLSLRLTFFISLSTILAFIAFTWFMLHSVENHFAEQDVSDLQQISTTLNRILQSPEDPDEKKVSKIKESI
ASYRNVALLLLNPRGEVLFSSAQGAALRPAVNSADFSEHSHARDVFLWTVEDPARPMDTGSEMKMETYRIIASSGQAIFQ
GKQQNYVMLTGLSINFHLHYLDALKKNLIAIAVVISLLIVLIIRIAVRQGHLPLRNVSNAIKSITSENLDTRLEPTRVPI
ELEQLVISFNHMIGKIEDVFTRQANFSADIAHEIRTPITNLVTQTEIALSQDRTQKELEDVLYSSLEEYNRMTKMVSDML
FLAQADNNQLIPDRVRFDLRAEVMKVFEFFEAWAEERNITLKFNGMPCLVEGDPQMFRRAINNLLSNALRYTPEGQAITV
SIREQESFFDLVIENPGKPIPEEHLSRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVEAHHGRVQVESDVRSTRFILSVP
RLEQRIPEPQH

Specific function: Component of the sil cation-efflux system that confers resistance to silver. Probable member of a two-component regulatory system silS/silR. May activate silR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=480, Percent_Identity=56.25, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI1788279, Length=310, Percent_Identity=29.6774193548387, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1788393, Length=254, Percent_Identity=31.496062992126, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786600, Length=222, Percent_Identity=35.5855855855856, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1790346, Length=271, Percent_Identity=29.520295202952, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1789403, Length=342, Percent_Identity=26.0233918128655, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1787894, Length=362, Percent_Identity=25.9668508287293, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790551, Length=282, Percent_Identity=27.6595744680851, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI145693157, Length=252, Percent_Identity=23.8095238095238, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788713, Length=228, Percent_Identity=28.0701754385965, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789149, Length=213, Percent_Identity=27.6995305164319, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081816, Length=258, Percent_Identity=27.5193798449612, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=25.4545454545455, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789808, Length=243, Percent_Identity=27.1604938271605, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790861, Length=225, Percent_Identity=28, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI48994928, Length=228, Percent_Identity=25.8771929824561, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1787374, Length=274, Percent_Identity=22.992700729927, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082128, Length=213, Percent_Identity=26.2910798122066, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56006; Mature: 56006

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSKPSRHPFSLSLRLTFFISLSTILAFIAFTWFMLHSVENHFAEQDVSDLQQISTTLNR
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ILQSPEDPDEKKVSKIKESIASYRNVALLLLNPRGEVLFSSAQGAALRPAVNSADFSEHS
HHCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCEEEECCCCCEECCCCCCCCCCCCC
HARDVFLWTVEDPARPMDTGSEMKMETYRIIASSGQAIFQGKQQNYVMLTGLSINFHLHY
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCEEEEEEEEEEEEHHH
LDALKKNLIAIAVVISLLIVLIIRIAVRQGHLPLRNVSNAIKSITSENLDTRLEPTRVPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCH
ELEQLVISFNHMIGKIEDVFTRQANFSADIAHEIRTPITNLVTQTEIALSQDRTQKELED
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLYSSLEEYNRMTKMVSDMLFLAQADNNQLIPDRVRFDLRAEVMKVFEFFEAWAEERNIT
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LKFNGMPCLVEGDPQMFRRAINNLLSNALRYTPEGQAITVSIREQESFFDLVIENPGKPI
EEECCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHEECCCCCCC
PEEHLSRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVEAHHGRVQVESDVRSTRFILSVP
CHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHHEEECC
RLEQRIPEPQH
HHHHCCCCCCC
>Mature Secondary Structure
MHSKPSRHPFSLSLRLTFFISLSTILAFIAFTWFMLHSVENHFAEQDVSDLQQISTTLNR
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ILQSPEDPDEKKVSKIKESIASYRNVALLLLNPRGEVLFSSAQGAALRPAVNSADFSEHS
HHCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCEEEECCCCCEECCCCCCCCCCCCC
HARDVFLWTVEDPARPMDTGSEMKMETYRIIASSGQAIFQGKQQNYVMLTGLSINFHLHY
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCEEEEEEEEEEEEHHH
LDALKKNLIAIAVVISLLIVLIIRIAVRQGHLPLRNVSNAIKSITSENLDTRLEPTRVPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCH
ELEQLVISFNHMIGKIEDVFTRQANFSADIAHEIRTPITNLVTQTEIALSQDRTQKELED
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLYSSLEEYNRMTKMVSDMLFLAQADNNQLIPDRVRFDLRAEVMKVFEFFEAWAEERNIT
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LKFNGMPCLVEGDPQMFRRAINNLLSNALRYTPEGQAITVSIREQESFFDLVIENPGKPI
EEECCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHEECCCCCCC
PEEHLSRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVEAHHGRVQVESDVRSTRFILSVP
CHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHHEEECC
RLEQRIPEPQH
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9930866 [H]