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The map label for this gene is yhfE [H]

Identifier: 116872383

GI number: 116872383

Start: 988572

End: 989624

Strand: Direct

Name: yhfE [H]

Synonym: lwe0965

Alternate gene names: 116872383

Gene position: 988572-989624 (Clockwise)

Preceding gene: 116872381

Following gene: 116872384

Centisome position: 35.13

GC content: 36.47

Gene sequence:

>1053_bases
TTGTCATACGAACCAAATACAAAACAAACAATGCAAAAGATTAAAGAATTAACATCTATCCCAAGCCCAACTGGTAACAC
TGGGAAAATTATTGAAACAATTTCCAAGGAGTTAGACAGTGCCAACATTCCTTATAGCTTAAATAATAAAGGTGGTTTAA
TCGTTACGCTTCCGGGCAAAGATGATACAAAACATCGTATGCTAACGGCGCATGTGGATACACTTGGGGCCATGGTTAAA
GAAATTAAATCTGATGGAAGATTACTTTTAACTTTAATTGGTGGCTACCGTTTTAACGCAATTGAAGGTGAATATTGTAC
GATTGAAACAAGTGCTGGAGATTTGTATAGCGGAACTATTCTTATGCACCAAACTTCTGTCCATGTGTATAAAGATGCTG
GAACTGCTGAACGTAATGATAAAAATATGGAAGTTCGTCTAGATGTGAGAGCGCTAGATGCAGATAAAGTACGTGCGCTT
GGAATTGAAGTAGGGGACTTTGTTTCCTTTGATCCACGTGTCCAAATTATTAATGATGAGTATATTAAATCTCGCCATTT
AGATGATAAAGCAAGTGTCGCGATTTTATTACAACTAATTAATTACATTCACGAACAAAAACTAGAGTTGCCACACACAA
CCCACTTCCTTATCTCTAACAATGAAGAAATCGGTTATGGTGGAAATTCTAATATTCCAGTCGAAACAGTGGAGTATCTG
GCTGTTGATATGGGAGCGCTTGGTGATGGACAAACATCGGATGAATACACGGTTTCCATTTGTGCAAAAGATGGTAGTGG
TCCTTATCACCTTGGTCTGCGTAAACATTTAGTGGAACTTGCTAAAAAGAATCATATCGACTATAAAATAGATATTTATC
CGTTTTACGCATCGGATGCAAGTGCGGCGATTAATGCTGGTAATGATATTATTCACGGTTTAATTGGACCAGGAATTGAT
GCTAGTCATGCTTATGAGCGTACACATCGAGATTCACTTTATCATACAGAAAAATTAGTTTATGCTTATTTATTTTCAAA
TATAATAAAATAG

Upstream 100 bases:

>100_bases
AAAACGAAAGAAATGCGAAAAAGTTAATCCTAAGAAATGCGCTCCATAAAGATAAGCGCTATAATATAATAGTAGAAAGT
GAACTAAAGGAGGAATACGC

Downstream 100 bases:

>100_bases
GAGGAATTCCATATGAATGTCCATGATTTTTCTGAAAAAGCAATGAATGGTAAAGAAATATCCTTAAGTGATTATAAAGG
TAAAGTTTTATTAATCGTTA

Product: metallopeptidase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSYEPNTKQTMQKIKELTSIPSPTGNTGKIIETISKELDSANIPYSLNNKGGLIVTLPGKDDTKHRMLTAHVDTLGAMVK
EIKSDGRLLLTLIGGYRFNAIEGEYCTIETSAGDLYSGTILMHQTSVHVYKDAGTAERNDKNMEVRLDVRALDADKVRAL
GIEVGDFVSFDPRVQIINDEYIKSRHLDDKASVAILLQLINYIHEQKLELPHTTHFLISNNEEIGYGGNSNIPVETVEYL
AVDMGALGDGQTSDEYTVSICAKDGSGPYHLGLRKHLVELAKKNHIDYKIDIYPFYASDASAAINAGNDIIHGLIGPGID
ASHAYERTHRDSLYHTEKLVYAYLFSNIIK

Sequences:

>Translated_350_residues
MSYEPNTKQTMQKIKELTSIPSPTGNTGKIIETISKELDSANIPYSLNNKGGLIVTLPGKDDTKHRMLTAHVDTLGAMVK
EIKSDGRLLLTLIGGYRFNAIEGEYCTIETSAGDLYSGTILMHQTSVHVYKDAGTAERNDKNMEVRLDVRALDADKVRAL
GIEVGDFVSFDPRVQIINDEYIKSRHLDDKASVAILLQLINYIHEQKLELPHTTHFLISNNEEIGYGGNSNIPVETVEYL
AVDMGALGDGQTSDEYTVSICAKDGSGPYHLGLRKHLVELAKKNHIDYKIDIYPFYASDASAAINAGNDIIHGLIGPGID
ASHAYERTHRDSLYHTEKLVYAYLFSNIIK
>Mature_349_residues
SYEPNTKQTMQKIKELTSIPSPTGNTGKIIETISKELDSANIPYSLNNKGGLIVTLPGKDDTKHRMLTAHVDTLGAMVKE
IKSDGRLLLTLIGGYRFNAIEGEYCTIETSAGDLYSGTILMHQTSVHVYKDAGTAERNDKNMEVRLDVRALDADKVRALG
IEVGDFVSFDPRVQIINDEYIKSRHLDDKASVAILLQLINYIHEQKLELPHTTHFLISNNEEIGYGGNSNIPVETVEYLA
VDMGALGDGQTSDEYTVSICAKDGSGPYHLGLRKHLVELAKKNHIDYKIDIYPFYASDASAAINAGNDIIHGLIGPGIDA
SHAYERTHRDSLYHTEKLVYAYLFSNIIK

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1788727, Length=165, Percent_Identity=26.6666666666667, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: NA

Molecular weight: Translated: 38742; Mature: 38611

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYEPNTKQTMQKIKELTSIPSPTGNTGKIIETISKELDSANIPYSLNNKGGLIVTLPGK
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCC
DDTKHRMLTAHVDTLGAMVKEIKSDGRLLLTLIGGYRFNAIEGEYCTIETSAGDLYSGTI
CCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCCEEEEECCCCCEEECEE
LMHQTSVHVYKDAGTAERNDKNMEVRLDVRALDADKVRALGIEVGDFVSFDPRVQIINDE
EEEECEEEEEECCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCCEEEEECCH
YIKSRHLDDKASVAILLQLINYIHEQKLELPHTTHFLISNNEEIGYGGNSNIPVETVEYL
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEECCCCCCCCCHHHHHHH
AVDMGALGDGQTSDEYTVSICAKDGSGPYHLGLRKHLVELAKKNHIDYKIDIYPFYASDA
HHHHCCCCCCCCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
SAAINAGNDIIHGLIGPGIDASHAYERTHRDSLYHTEKLVYAYLFSNIIK
CCEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SYEPNTKQTMQKIKELTSIPSPTGNTGKIIETISKELDSANIPYSLNNKGGLIVTLPGK
CCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCC
DDTKHRMLTAHVDTLGAMVKEIKSDGRLLLTLIGGYRFNAIEGEYCTIETSAGDLYSGTI
CCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCCEEEEECCCCCEEECEE
LMHQTSVHVYKDAGTAERNDKNMEVRLDVRALDADKVRALGIEVGDFVSFDPRVQIINDE
EEEECEEEEEECCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCCEEEEECCH
YIKSRHLDDKASVAILLQLINYIHEQKLELPHTTHFLISNNEEIGYGGNSNIPVETVEYL
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEECCCCCCCCCHHHHHHH
AVDMGALGDGQTSDEYTVSICAKDGSGPYHLGLRKHLVELAKKNHIDYKIDIYPFYASDA
HHHHCCCCCCCCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
SAAINAGNDIIHGLIGPGIDASHAYERTHRDSLYHTEKLVYAYLFSNIIK
CCEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]