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The map label for this gene is nfrA1 [H]

Identifier: 116872333

GI number: 116872333

Start: 945681

End: 946421

Strand: Direct

Name: nfrA1 [H]

Synonym: lwe0915

Alternate gene names: 116872333

Gene position: 945681-946421 (Clockwise)

Preceding gene: 116872332

Following gene: 116872339

Centisome position: 33.6

GC content: 36.57

Gene sequence:

>741_bases
ATGAATCAGGCTATTGATGCCATTTTAGGGCATTATTCTGTAAGGAAATTTGAAGATAAATCTCTAACGGAAGAAGAATT
ATCTTTACTTATTAAAAGTGCACAGGCGGCTTCTACGTCAAGTTTTGTTCAAGCATACTCGATTATAGGCATCACTGACA
AAGAAGTGCGCAAACAAATCTCTTTAGTTGCAGGAAATCAACCATATACTGTACAAACAGGTCAATTATTTATATTTGTA
GCTGACTTAGCTAGACATCATGCTATCTTAGAAGAGTTCCAAGTAGATACAGAAGCATTAGAAACCTCAGAAAAATGGCT
GGTTTCAGTAATTGATGCTGCACTTGCCGCACAAAATATGGCCATCGCAGCAGAATCATTAGGTTTTGGAATTTGTTACA
TTGGCGGAATTCGCAATAATGTCGAACAAATCTCAAAAATTCTGGATTTGCCACCTTATACTATGCCGCTCTTTGGCTTA
ACAGTAGGGCATCCTGTAGTAGACAAAGAAAAAGCCAAACCAAGGTTACCCCAAAGTTTGGTATATCACGAAAATACTTA
CCAAAAAACCAACCCAACGATTCTAGCCGATTATGATGAGCAAATAAAAATGTATTATAACGAGAGAACAGCAGGCAAAC
GAATAGAAGGTTGGTCAGAACAAATGGCACGCGGATTAGGACAAAAAAATCGTCTAGACTTAAAAGCATTTTTAGAAAAG
CAACATTTAAATCAAAAATAA

Upstream 100 bases:

>100_bases
CAGTTTTAGCTTATGAAGCGTTACGTCAACAACAATTCGGCACACTATTACAACATCCAAATTATGATCGGAAGATTTTC
CTAGACTAGGAGGAGTTAGA

Downstream 100 bases:

>100_bases
AAAGAACGGTTACGCGCATTCATTAAAAGCGCGCAACCGTTCTTTTTTTAGTTATTTACGTTTATTCCTAGCCATTTGAA
TTAAGTTCCAAATAACGAGT

Product: nitroreductase family protein

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MNQAIDAILGHYSVRKFEDKSLTEEELSLLIKSAQAASTSSFVQAYSIIGITDKEVRKQISLVAGNQPYTVQTGQLFIFV
ADLARHHAILEEFQVDTEALETSEKWLVSVIDAALAAQNMAIAAESLGFGICYIGGIRNNVEQISKILDLPPYTMPLFGL
TVGHPVVDKEKAKPRLPQSLVYHENTYQKTNPTILADYDEQIKMYYNERTAGKRIEGWSEQMARGLGQKNRLDLKAFLEK
QHLNQK

Sequences:

>Translated_246_residues
MNQAIDAILGHYSVRKFEDKSLTEEELSLLIKSAQAASTSSFVQAYSIIGITDKEVRKQISLVAGNQPYTVQTGQLFIFV
ADLARHHAILEEFQVDTEALETSEKWLVSVIDAALAAQNMAIAAESLGFGICYIGGIRNNVEQISKILDLPPYTMPLFGL
TVGHPVVDKEKAKPRLPQSLVYHENTYQKTNPTILADYDEQIKMYYNERTAGKRIEGWSEQMARGLGQKNRLDLKAFLEK
QHLNQK
>Mature_246_residues
MNQAIDAILGHYSVRKFEDKSLTEEELSLLIKSAQAASTSSFVQAYSIIGITDKEVRKQISLVAGNQPYTVQTGQLFIFV
ADLARHHAILEEFQVDTEALETSEKWLVSVIDAALAAQNMAIAAESLGFGICYIGGIRNNVEQISKILDLPPYTMPLFGL
TVGHPVVDKEKAKPRLPQSLVYHENTYQKTNPTILADYDEQIKMYYNERTAGKRIEGWSEQMARGLGQKNRLDLKAFLEK
QHLNQK

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=241, Percent_Identity=42.3236514522822, Blast_Score=187, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 27603; Mature: 27603

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQAIDAILGHYSVRKFEDKSLTEEELSLLIKSAQAASTSSFVQAYSIIGITDKEVRKQI
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
SLVAGNQPYTVQTGQLFIFVADLARHHAILEEFQVDTEALETSEKWLVSVIDAALAAQNM
HHHCCCCCEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIAAESLGFGICYIGGIRNNVEQISKILDLPPYTMPLFGLTVGHPVVDKEKAKPRLPQSL
HHHHHHCCCEEEEECCHHCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHH
VYHENTYQKTNPTILADYDEQIKMYYNERTAGKRIEGWSEQMARGLGQKNRLDLKAFLEK
HHCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHH
QHLNQK
HHCCCC
>Mature Secondary Structure
MNQAIDAILGHYSVRKFEDKSLTEEELSLLIKSAQAASTSSFVQAYSIIGITDKEVRKQI
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
SLVAGNQPYTVQTGQLFIFVADLARHHAILEEFQVDTEALETSEKWLVSVIDAALAAQNM
HHHCCCCCEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIAAESLGFGICYIGGIRNNVEQISKILDLPPYTMPLFGLTVGHPVVDKEKAKPRLPQSL
HHHHHHCCCEEEEECCHHCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHH
VYHENTYQKTNPTILADYDEQIKMYYNERTAGKRIEGWSEQMARGLGQKNRLDLKAFLEK
HHCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHH
QHLNQK
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]