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The map label for this gene is yibK [C]

Identifier: 116872332

GI number: 116872332

Start: 945160

End: 945669

Strand: Direct

Name: yibK [C]

Synonym: lwe0914

Alternate gene names: 116872332

Gene position: 945160-945669 (Clockwise)

Preceding gene: 116872331

Following gene: 116872333

Centisome position: 33.59

GC content: 35.49

Gene sequence:

>510_bases
ATGCCAAATCATATCGTACTATATCAACCAGAAATCCCTGCAAATACCGGAAATATCTCCAGAACTTGTGCTGGAACAGA
TACTTATTTACATTTAATTCGTCCACTTGGTTTTTCAACAGACGATAAAATGTTAAAACGTGCTGGACTGGACTACTGGG
ATAATGTCAAACTTTCTTATTACGATTCTCTAGATGAGTTTTTCGAGAAAAATGCAGGTGGGGAATTTTACTATATTACC
AAATTTGGTCGCCATGTTTATAGTGACATTGATTATAGTGATCCAAATAAAGATTATTTCTTTGTTTTTGGGAAAGAAAC
GACGGGGTTACCAGATGAACTTTTACAAGAAAATGAAGAAAATTGTCTACGAATTCCGATGACAGATCATATTCGCTCGC
TGAATCTATCTAATACTGCTGCAGTTTTAGCTTATGAAGCGTTACGTCAACAACAATTCGGCACACTATTACAACATCCA
AATTATGATCGGAAGATTTTCCTAGACTAG

Upstream 100 bases:

>100_bases
CAGAAGAAGCAGTTAGAGAGGCCCTTCAAACCGAACAAGACGAACAAGTTTTACAAGAATTAACAGCTATACCAAATTAA
TAGAAACCAGGTGAAAAGAA

Downstream 100 bases:

>100_bases
GAGGAGTTAGAATGAATCAGGCTATTGATGCCATTTTAGGGCATTATTCTGTAAGGAAATTTGAAGATAAATCTCTAACG
GAAGAAGAATTATCTTTACT

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MPNHIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFEKNAGGEFYYIT
KFGRHVYSDIDYSDPNKDYFFVFGKETTGLPDELLQENEENCLRIPMTDHIRSLNLSNTAAVLAYEALRQQQFGTLLQHP
NYDRKIFLD

Sequences:

>Translated_169_residues
MPNHIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFEKNAGGEFYYIT
KFGRHVYSDIDYSDPNKDYFFVFGKETTGLPDELLQENEENCLRIPMTDHIRSLNLSNTAAVLAYEALRQQQFGTLLQHP
NYDRKIFLD
>Mature_168_residues
PNHIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFEKNAGGEFYYITK
FGRHVYSDIDYSDPNKDYFFVFGKETTGLPDELLQENEENCLRIPMTDHIRSLNLSNTAAVLAYEALRQQQFGTLLQHPN
YDRKIFLD

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=157, Percent_Identity=42.6751592356688, Blast_Score=123, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 19625; Mature: 19494

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNHIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSY
CCCCEEEECCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEE
YDSLDEFFEKNAGGEFYYITKFGRHVYSDIDYSDPNKDYFFVFGKETTGLPDELLQENEE
HHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHHHCCCC
NCLRIPMTDHIRSLNLSNTAAVLAYEALRQQQFGTLLQHPNYDRKIFLD
CEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
>Mature Secondary Structure 
PNHIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSY
CCCEEEECCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEE
YDSLDEFFEKNAGGEFYYITKFGRHVYSDIDYSDPNKDYFFVFGKETTGLPDELLQENEE
HHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHHHCCCC
NCLRIPMTDHIRSLNLSNTAAVLAYEALRQQQFGTLLQHPNYDRKIFLD
CEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]