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The map label for this gene is sdhA [C]

Identifier: 116689993

GI number: 116689993

Start: 2190453

End: 2192192

Strand: Direct

Name: sdhA [C]

Synonym: Bcen2424_1972

Alternate gene names: 116689993

Gene position: 2190453-2192192 (Clockwise)

Preceding gene: 116689992

Following gene: 116689994

Centisome position: 62.87

GC content: 66.03

Gene sequence:

>1740_bases
ATGAATACCCATGTGCATGAATACGACGTCGTCGTGGTCGGCGGCGGCACGGCCGGCCCGATGGCGGCCATCAAGGCGAA
GGAGCGCGATCCGTCGCTGCGCGTGCTGCTGCTCGAGAAGGCCAACGTGAAACGCAGCGGCGCGATCTCGATGGGGATGG
ACGGCCTGAACAACGCGGTGATCCCCGGTCATGCGACGCCCGAGCAATACACGCGCGAGATCACGATCGCCAACGACGGG
ATCGTCGACCAGGAAGCCGTGTACGCGTATGCGACGCACAGCTTCACGACGATCGAGCAGCTCGACCGCTGGGGCGTGAA
GTTCGAGAAGGACGGCACCGGCGACTACGCGGTGAAGAAGGTCCATCACATGGGCTCGTACGTGCTGCCGATGCCGGAGG
GACACGACATCAAGAAGGTGCTGTACCGGCAACTGAAGCGCGCACGCATCGCGATCACGAACCGGATCGTCGCGACGCGC
GTGCTGACCGACGCACAAGGCCGCGCGAGCGGCGTGCTCGGTTTCGACTGCCGCACCGCCGACTTCCACGTGATCCGCGC
GAAGGCCGTGATCCTCTGCTGCGGCGCGGCCGGCCGCCTCGGCCTGCCGGCATCGGGCTACCTGATGGGCACGTACGAGA
ACCCGACCAACGCGGGCGACGGCTACGCGATGGCCTATCACGCGGGCGCCGCGCTCGCGAACCTCGAATGCTTCCAGATC
AATCCGCTGATCAAAGATTACAACGGCCCGGCGTGCGCGTACGTGACCGGCCCGCTCGGCGGCTTCACCGCGAACGGCAA
GGGCGAACGCTTCATCGAATGCGACTACTGGAGCGGCCAGATGATGTGGGAGTTCTACCAGGAACTGCAGAGCGGCAACG
GCCCCGTGTTCCTGAAGCTCGACCATCTCGCGGAAGAGACGATCAGGACCATCGAGCAGATCCTGCATACCAACGAACGG
CCCAGCCGCGGCCGCTTCCATGCGGGGCGCGGCACCGACTACCGCAGCCAGATGGTCGAGATGCACATCTCCGAGATCGG
CTTCTGCAGCGGCCACAGCGCGTCGGGCGTGTACGTGAACGCACGTGCCGAGACGACCGTGCCGGGGCTTTACGCGGCCG
GCGACATGGCGGCCGTCCCGCACAACTACATGCTCGGGGCATTCACGTACGGCTGGTTCGCCGGACAAAACGCAGCTGAC
TACGTGGCCGGCCGCGACCACGCGCCGGTGGACGACCAACAGGTCAACGCCGAGCGCACCCGCGTGCTCGCACCGCTGCT
GCGCGAACGCGGGCTGGCGCCGGCGCAGGTCGAGTACAAGCTGCGCCGGATGGTGAACGACTACCTGCAACCGCCGAAGG
TGACGCGCAAGATGGAGATCGGGCTACAACGTTTTGACGGAATTATTGAGGATATCGAAGAAATCAAGGCCGACAATCCG
CACGAACTGATGCGTGCGGCCGAAGTGCGCGCGATCCGCGATTGCGCCGAAATGGCCGCGCGCGCGTCGCTGTTCCGCAC
CGAAAGCCGCTGGGGCCTGTACCACCATCGCGTCGATTTTCCGCACCGCAACGACGCCGAATGGTTCTGCCACACGTGGT
TGCGCAAGGATGCCGAGGGCGCGATGCGCAGCGAGAAGCGGCCGGTCGAACCGTACGTCGTGCCGCTCGCCGACGACGAA
CGCGGCGCCTACGCGCAGTTGCGCATCCGCGAACCGGCCGCGCTCGCGGCCGCCCTTTGA

Upstream 100 bases:

>100_bases
CGCCGATCGACTACGAATACCTCTACTTCCGCGGCGACGCCTTCCAGTACCGGTTGCGCGTCGACCGGCAAAAGGCGCGC
AAACCTGCAAGGAGCCCGCG

Downstream 100 bases:

>100_bases
GGAACCTTGCCATGTCCCATACCCCGCACGACATCTTCCAGCGCAGCAGCGCGCCCGTGACGATCGACGAGTCGCTGTGC
ATCGCCGACAAGGGCTGTAC

Product: putative oxidoreductase

Products: FADH2; Fumarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MNTHVHEYDVVVVGGGTAGPMAAIKAKERDPSLRVLLLEKANVKRSGAISMGMDGLNNAVIPGHATPEQYTREITIANDG
IVDQEAVYAYATHSFTTIEQLDRWGVKFEKDGTGDYAVKKVHHMGSYVLPMPEGHDIKKVLYRQLKRARIAITNRIVATR
VLTDAQGRASGVLGFDCRTADFHVIRAKAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
NPLIKDYNGPACAYVTGPLGGFTANGKGERFIECDYWSGQMMWEFYQELQSGNGPVFLKLDHLAEETIRTIEQILHTNER
PSRGRFHAGRGTDYRSQMVEMHISEIGFCSGHSASGVYVNARAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGQNAAD
YVAGRDHAPVDDQQVNAERTRVLAPLLRERGLAPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFDGIIEDIEEIKADNP
HELMRAAEVRAIRDCAEMAARASLFRTESRWGLYHHRVDFPHRNDAEWFCHTWLRKDAEGAMRSEKRPVEPYVVPLADDE
RGAYAQLRIREPAALAAAL

Sequences:

>Translated_579_residues
MNTHVHEYDVVVVGGGTAGPMAAIKAKERDPSLRVLLLEKANVKRSGAISMGMDGLNNAVIPGHATPEQYTREITIANDG
IVDQEAVYAYATHSFTTIEQLDRWGVKFEKDGTGDYAVKKVHHMGSYVLPMPEGHDIKKVLYRQLKRARIAITNRIVATR
VLTDAQGRASGVLGFDCRTADFHVIRAKAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
NPLIKDYNGPACAYVTGPLGGFTANGKGERFIECDYWSGQMMWEFYQELQSGNGPVFLKLDHLAEETIRTIEQILHTNER
PSRGRFHAGRGTDYRSQMVEMHISEIGFCSGHSASGVYVNARAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGQNAAD
YVAGRDHAPVDDQQVNAERTRVLAPLLRERGLAPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFDGIIEDIEEIKADNP
HELMRAAEVRAIRDCAEMAARASLFRTESRWGLYHHRVDFPHRNDAEWFCHTWLRKDAEGAMRSEKRPVEPYVVPLADDE
RGAYAQLRIREPAALAAAL
>Mature_579_residues
MNTHVHEYDVVVVGGGTAGPMAAIKAKERDPSLRVLLLEKANVKRSGAISMGMDGLNNAVIPGHATPEQYTREITIANDG
IVDQEAVYAYATHSFTTIEQLDRWGVKFEKDGTGDYAVKKVHHMGSYVLPMPEGHDIKKVLYRQLKRARIAITNRIVATR
VLTDAQGRASGVLGFDCRTADFHVIRAKAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
NPLIKDYNGPACAYVTGPLGGFTANGKGERFIECDYWSGQMMWEFYQELQSGNGPVFLKLDHLAEETIRTIEQILHTNER
PSRGRFHAGRGTDYRSQMVEMHISEIGFCSGHSASGVYVNARAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGQNAAD
YVAGRDHAPVDDQQVNAERTRVLAPLLRERGLAPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFDGIIEDIEEIKADNP
HELMRAAEVRAIRDCAEMAARASLFRTESRWGLYHHRVDFPHRNDAEWFCHTWLRKDAEGAMRSEKRPVEPYVVPLADDE
RGAYAQLRIREPAALAAAL

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786942, Length=604, Percent_Identity=24.6688741721854, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1788928, Length=565, Percent_Identity=23.1858407079646, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790597, Length=600, Percent_Identity=21.8333333333333, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1 [C]

Molecular weight: Translated: 64191; Mature: 64191

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTHVHEYDVVVVGGGTAGPMAAIKAKERDPSLRVLLLEKANVKRSGAISMGMDGLNNAV
CCCCEEEEEEEEECCCCCCCHHHEECCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCC
IPGHATPEQYTREITIANDGIVDQEAVYAYATHSFTTIEQLDRWGVKFEKDGTGDYAVKK
CCCCCCHHHHCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCEEECCCCCHHHHHH
VHHMGSYVLPMPEGHDIKKVLYRQLKRARIAITNRIVATRVLTDAQGRASGVLGFDCRTA
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
DFHVIRAKAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
CEEEEEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCHHHCEEEEEE
NPLIKDYNGPACAYVTGPLGGFTANGKGERFIECDYWSGQMMWEFYQELQSGNGPVFLKL
CCHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
DHLAEETIRTIEQILHTNERPSRGRFHAGRGTDYRSQMVEMHISEIGFCSGHSASGVYVN
HHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEE
ARAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGQNAADYVAGRDHAPVDDQQVNAERT
CCCCCCCCCEEECCCCCCCCCCCEEEEEEEEEECCCCCHHHCCCCCCCCCCCCCCCHHHH
RVLAPLLRERGLAPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFDGIIEDIEEIKADNP
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
HELMRAAEVRAIRDCAEMAARASLFRTESRWGLYHHRVDFPHRNDAEWFCHTWLRKDAEG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
AMRSEKRPVEPYVVPLADDERGAYAQLRIREPAALAAAL
HHHCCCCCCCCEEEEECCCCCCCEEEEEECCCHHHHHCC
>Mature Secondary Structure
MNTHVHEYDVVVVGGGTAGPMAAIKAKERDPSLRVLLLEKANVKRSGAISMGMDGLNNAV
CCCCEEEEEEEEECCCCCCCHHHEECCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCC
IPGHATPEQYTREITIANDGIVDQEAVYAYATHSFTTIEQLDRWGVKFEKDGTGDYAVKK
CCCCCCHHHHCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCEEECCCCCHHHHHH
VHHMGSYVLPMPEGHDIKKVLYRQLKRARIAITNRIVATRVLTDAQGRASGVLGFDCRTA
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
DFHVIRAKAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
CEEEEEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCHHHCEEEEEE
NPLIKDYNGPACAYVTGPLGGFTANGKGERFIECDYWSGQMMWEFYQELQSGNGPVFLKL
CCHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
DHLAEETIRTIEQILHTNERPSRGRFHAGRGTDYRSQMVEMHISEIGFCSGHSASGVYVN
HHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEE
ARAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGQNAADYVAGRDHAPVDDQQVNAERT
CCCCCCCCCEEECCCCCCCCCCCEEEEEEEEEECCCCCHHHCCCCCCCCCCCCCCCHHHH
RVLAPLLRERGLAPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFDGIIEDIEEIKADNP
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
HELMRAAEVRAIRDCAEMAARASLFRTESRWGLYHHRVDFPHRNDAEWFCHTWLRKDAEG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
AMRSEKRPVEPYVVPLADDERGAYAQLRIREPAALAAAL
HHHCCCCCCCCEEEEECCCCCCCEEEEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.3 {ferricyanide}} 0.4 {fumarate}} [C]

Substrates: FAD; Succinate [C]

Specific reaction: FAD + Succinate --> FADH2 + Fumarate [C]

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]