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The map label for this gene is psdht [H]

Identifier: 116689967

GI number: 116689967

Start: 2164079

End: 2165098

Strand: Direct

Name: psdht [H]

Synonym: Bcen2424_1946

Alternate gene names: 116689967

Gene position: 2164079-2165098 (Clockwise)

Preceding gene: 116689966

Following gene: 116689968

Centisome position: 62.12

GC content: 68.04

Gene sequence:

>1020_bases
ATGTCGACTGAACAACAGGGCACTGCCCATACGACGATCGACGGCGAGCCGATTCCGACGCTCGACGAAATCGCCGCGCA
GCATTTCGCGCTGTCACCCTGGGTCGCGCGCACGCCCGTGTTCGAGCGCGCCGATCTGCCCTCCCTCGAAGGCACGCACG
TCAACTTCAAGTTCGAGCTGTTGCAGGCGAGCGGCAGCTTCAAGGCGCGCGGCGCGTTCACGCACCTGCTCGCGCTCGAC
GAAGACCGCAAGAACGCGGGCGTCACCTGCGTATCGGCCGGCAATCATGCGGCAGCCGTCGCGTATGCGGCGATGCGGCT
CGGCAGCAGCGCGAAGGTCGTGATGTTCCATACCGCGAGCCCGACGCGCATCGCGCAGTGCAAGCAGTACCGCGCGGAAG
TCGTGCTGGCAGGCAGCGCGTCGCAGGCGTTCGATCTCGTGCGGCGCATCGAGGCCGAGGAAGGCCGCTTCTTCATTCAT
CCGTTCAACGGCTATCACACGATCCTCGGCACCGCGACGCTCGGCTACGAATGGGCGACGCAGACGCCGGACCTCGACGC
GGTGATCGTGCCGATCGGCGGCGGCGGGCTCGCGGCCGGCATGGCGACCGCGCTGCGGCTCGCGAATCCGCGTGTGCACG
TGTACGGCGTCGAGCCGGAAGGCGCCGACGCGATGAGCCGCAGCTTCGCGGCCAATCACACGATCAAGATGAGCGCGATG
CAGACGATCGCCGATTCGCTGATGGCGCCGCATACCGAGGAATACAGCTACCAGCTGTGCCGGCGCCACCTCGACCGCAT
CGTCACCGTCTCCGACGACGCGCTGCGCGCCGCGATGCTGTTCCTCTTCTCGCACCTGAAACTGTCGGTCGAGCCGGCCT
GCGCGGCCGCGCTCGCCGCGCTGCTCGGCCCGCTGCGCGAAACGCTGCAGGGCAAGCGCGTCGGCGTGCTGCTGTCGGGC
ACGAATACCGACCCCGCCACGCTCGGCATGCATCTCGCGCACGCGCAACTGCAGCACTGA

Upstream 100 bases:

>100_bases
GCGCAAGTTCATTCGCAGCATGTCGGTCGACCCGCACTGACTGCCTCCCGCCACGCCGGCCCGCGTGTGCGGGTCGGCCC
ACTCCGCCACCCGCCACGTC

Downstream 100 bases:

>100_bases
TCGCGCAGTGCGCCTGCGCCGGCCATCCTAGGGTTATCCCCAGATTATGTTGACAGCCCTGTTGATAACCCGCCGGACCC
GCACGCAAGTGGTTGAGCGC

Product: pyridoxal-5'-phosphate-dependent enzyme, beta subunit

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MSTEQQGTAHTTIDGEPIPTLDEIAAQHFALSPWVARTPVFERADLPSLEGTHVNFKFELLQASGSFKARGAFTHLLALD
EDRKNAGVTCVSAGNHAAAVAYAAMRLGSSAKVVMFHTASPTRIAQCKQYRAEVVLAGSASQAFDLVRRIEAEEGRFFIH
PFNGYHTILGTATLGYEWATQTPDLDAVIVPIGGGGLAAGMATALRLANPRVHVYGVEPEGADAMSRSFAANHTIKMSAM
QTIADSLMAPHTEEYSYQLCRRHLDRIVTVSDDALRAAMLFLFSHLKLSVEPACAAALAALLGPLRETLQGKRVGVLLSG
TNTDPATLGMHLAHAQLQH

Sequences:

>Translated_339_residues
MSTEQQGTAHTTIDGEPIPTLDEIAAQHFALSPWVARTPVFERADLPSLEGTHVNFKFELLQASGSFKARGAFTHLLALD
EDRKNAGVTCVSAGNHAAAVAYAAMRLGSSAKVVMFHTASPTRIAQCKQYRAEVVLAGSASQAFDLVRRIEAEEGRFFIH
PFNGYHTILGTATLGYEWATQTPDLDAVIVPIGGGGLAAGMATALRLANPRVHVYGVEPEGADAMSRSFAANHTIKMSAM
QTIADSLMAPHTEEYSYQLCRRHLDRIVTVSDDALRAAMLFLFSHLKLSVEPACAAALAALLGPLRETLQGKRVGVLLSG
TNTDPATLGMHLAHAQLQH
>Mature_338_residues
STEQQGTAHTTIDGEPIPTLDEIAAQHFALSPWVARTPVFERADLPSLEGTHVNFKFELLQASGSFKARGAFTHLLALDE
DRKNAGVTCVSAGNHAAAVAYAAMRLGSSAKVVMFHTASPTRIAQCKQYRAEVVLAGSASQAFDLVRRIEAEEGRFFIHP
FNGYHTILGTATLGYEWATQTPDLDAVIVPIGGGGLAAGMATALRLANPRVHVYGVEPEGADAMSRSFAANHTIKMSAMQ
TIADSLMAPHTEEYSYQLCRRHLDRIVTVSDDALRAAMLFLFSHLKLSVEPACAAALAALLGPLRETLQGKRVGVLLSGT
NTDPATLGMHLAHAQLQH

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=309, Percent_Identity=31.0679611650485, Blast_Score=147, Evalue=1e-35,
Organism=Escherichia coli, GI1789505, Length=271, Percent_Identity=38.0073800738007, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1790207, Length=304, Percent_Identity=32.2368421052632, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71991565, Length=308, Percent_Identity=35.7142857142857, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17508781, Length=317, Percent_Identity=28.391167192429, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17537387, Length=308, Percent_Identity=32.1428571428571, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322631, Length=309, Percent_Identity=34.6278317152104, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6320930, Length=274, Percent_Identity=30.6569343065693, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6319788, Length=284, Percent_Identity=23.943661971831, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355833, Length=311, Percent_Identity=35.6913183279743, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24645328, Length=219, Percent_Identity=35.1598173515982, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 36338; Mature: 36207

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEQQGTAHTTIDGEPIPTLDEIAAQHFALSPWVARTPVFERADLPSLEGTHVNFKFEL
CCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCEEEEEEEE
LQASGSFKARGAFTHLLALDEDRKNAGVTCVSAGNHAAAVAYAAMRLGSSAKVVMFHTAS
EECCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCC
PTRIAQCKQYRAEVVLAGSASQAFDLVRRIEAEEGRFFIHPFNGYHTILGTATLGYEWAT
CHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEECEEECC
QTPDLDAVIVPIGGGGLAAGMATALRLANPRVHVYGVEPEGADAMSRSFAANHTIKMSAM
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCEEEHHHH
QTIADSLMAPHTEEYSYQLCRRHLDRIVTVSDDALRAAMLFLFSHLKLSVEPACAAALAA
HHHHHHHHCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHCEEECHHHHHHHHH
LLGPLRETLQGKRVGVLLSGTNTDPATLGMHLAHAQLQH
HHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STEQQGTAHTTIDGEPIPTLDEIAAQHFALSPWVARTPVFERADLPSLEGTHVNFKFEL
CCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCEEEEEEEE
LQASGSFKARGAFTHLLALDEDRKNAGVTCVSAGNHAAAVAYAAMRLGSSAKVVMFHTAS
EECCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCC
PTRIAQCKQYRAEVVLAGSASQAFDLVRRIEAEEGRFFIHPFNGYHTILGTATLGYEWAT
CHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEECEEECC
QTPDLDAVIVPIGGGGLAAGMATALRLANPRVHVYGVEPEGADAMSRSFAANHTIKMSAM
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCEEEHHHH
QTIADSLMAPHTEEYSYQLCRRHLDRIVTVSDDALRAAMLFLFSHLKLSVEPACAAALAA
HHHHHHHHCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHCEEECHHHHHHHHH
LLGPLRETLQGKRVGVLLSGTNTDPATLGMHLAHAQLQH
HHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]