Click here to switch to the map view.

The map label for this gene is ygfZ [C]

Identifier: 116689916

GI number: 116689916

Start: 2099611

End: 2100645

Strand: Direct

Name: ygfZ [C]

Synonym: Bcen2424_1895

Alternate gene names: 116689916

Gene position: 2099611-2100645 (Clockwise)

Preceding gene: 116689906

Following gene: 116689917

Centisome position: 60.27

GC content: 71.69

Gene sequence:

>1035_bases
ATGAGCACACCGTTCGCTTCACCGGCTGCCCAGGCCGCATCTGCCTCGCTCCCCGTTTTCCCCCGCCCGTCCGCCGCCGA
TTTCGATGCGCCGGCCGCCTGCATGCCGCTGCCGCAGTTCGGCGTGATCGACGTGGCCGGCGACGATGCCGCGACGTTCC
TGCACAGCCAGCTCACCAACGACATCGAGCATCTCGATGCCGGCAGCGCCCGGCTGTCCGGGTACTGCTCGCCGAAGGGC
CGCCTGCTCGCGTCGTTCCTCACGTGGCGCGCCGGCCACGATGTGCGCCTGCTCGTGTCGAAGGACGTGCAGCCGGCCGT
GCAGAAGCGGCTGTCGATGTTCGTGCTGCGCGCGAAGGCGAAGCTGACGGACGCGAGCGACACGCTCGCGGTGGCCGGCT
TCGCGGGCGACGTGCGCGACGCGCTGTCCGGCATCTTCGATGCGCTGCCCGACGGCGTGCACGTGAAGGTCGACGGCCCG
GCCGGCGCGCTGATCCGCGTGCCCGATGCGGCTGGCCGCAAGCGCTATCTGTGGATCGGCCCGCGCGCCGAGGTCGACGC
GCGCCTCGCCGCGCTGGCCGGCACGCTGCCGGTCGTGTCGCCCGCCGTGTGGGACTGGCTCGACGTTCGCGCGGGCGAGC
CGCGCATCACGCAGCCGGCCGTCGAACAGTTCGTACCGCAAATGGTCAACTTCGACGTGATCGGTGCCGTTAACTTCAGG
AAAGGGTGCTACCCGGGCCAGGAAGTGGTGGCGCGCAGCCAATACCGCGGCACGATCAAGCGCCGCACCGCGCTCGCGCA
CGTCGCGGGCGAAACCGATACCGTGCATGCCGGCGTCGAGTTGTTCCACAGCGACGACCCGGGCCAGCCGTGCGGGATGA
TCGTCAACGCGGCGGCCGCGCCGGCGGGCGGCGTCGACGCGCTCGTCGAGATCAAGCTCGCCGCGCTCGACGGCGGCACG
GTGCACCTCGGTTCGGCCGACGGCCCGGCGCTCGCGTTCGACGCGCTGCCGTACGCGTGGCCGACCGAAGTCTGA

Upstream 100 bases:

>100_bases
GCGCATTTCTTCAGTAGGGACATGGAAACGTGACTCAGGTAAGACCCATATAATACTTGCTCGCCTCCGTCAAAGTCAGG
GTTGACTGTTCCCTCATTCC

Downstream 100 bases:

>100_bases
TGCACTGACAACCCGTTCGGGCGCCGGCTCGCGAGTCGCAGCCGGCGCCCGACGTTTCCTGCGAGAGATTCGCCCGATGT
GTCTGATTGCCTTCGACTGG

Product: glycine cleavage T protein (aminomethyl transferase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSTPFASPAAQAASASLPVFPRPSAADFDAPAACMPLPQFGVIDVAGDDAATFLHSQLTNDIEHLDAGSARLSGYCSPKG
RLLASFLTWRAGHDVRLLVSKDVQPAVQKRLSMFVLRAKAKLTDASDTLAVAGFAGDVRDALSGIFDALPDGVHVKVDGP
AGALIRVPDAAGRKRYLWIGPRAEVDARLAALAGTLPVVSPAVWDWLDVRAGEPRITQPAVEQFVPQMVNFDVIGAVNFR
KGCYPGQEVVARSQYRGTIKRRTALAHVAGETDTVHAGVELFHSDDPGQPCGMIVNAAAAPAGGVDALVEIKLAALDGGT
VHLGSADGPALAFDALPYAWPTEV

Sequences:

>Translated_344_residues
MSTPFASPAAQAASASLPVFPRPSAADFDAPAACMPLPQFGVIDVAGDDAATFLHSQLTNDIEHLDAGSARLSGYCSPKG
RLLASFLTWRAGHDVRLLVSKDVQPAVQKRLSMFVLRAKAKLTDASDTLAVAGFAGDVRDALSGIFDALPDGVHVKVDGP
AGALIRVPDAAGRKRYLWIGPRAEVDARLAALAGTLPVVSPAVWDWLDVRAGEPRITQPAVEQFVPQMVNFDVIGAVNFR
KGCYPGQEVVARSQYRGTIKRRTALAHVAGETDTVHAGVELFHSDDPGQPCGMIVNAAAAPAGGVDALVEIKLAALDGGT
VHLGSADGPALAFDALPYAWPTEV
>Mature_343_residues
STPFASPAAQAASASLPVFPRPSAADFDAPAACMPLPQFGVIDVAGDDAATFLHSQLTNDIEHLDAGSARLSGYCSPKGR
LLASFLTWRAGHDVRLLVSKDVQPAVQKRLSMFVLRAKAKLTDASDTLAVAGFAGDVRDALSGIFDALPDGVHVKVDGPA
GALIRVPDAAGRKRYLWIGPRAEVDARLAALAGTLPVVSPAVWDWLDVRAGEPRITQPAVEQFVPQMVNFDVIGAVNFRK
GCYPGQEVVARSQYRGTIKRRTALAHVAGETDTVHAGVELFHSDDPGQPCGMIVNAAAAPAGGVDALVEIKLAALDGGTV
HLGSADGPALAFDALPYAWPTEV

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=255, Percent_Identity=28.6274509803922, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 35999; Mature: 35868

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPFASPAAQAASASLPVFPRPSAADFDAPAACMPLPQFGVIDVAGDDAATFLHSQLTN
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEEECCCHHHHHHHHHHHH
DIEHLDAGSARLSGYCSPKGRLLASFLTWRAGHDVRLLVSKDVQPAVQKRLSMFVLRAKA
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
KLTDASDTLAVAGFAGDVRDALSGIFDALPDGVHVKVDGPAGALIRVPDAAGRKRYLWIG
HCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCCCCCEEEEEEC
PRAEVDARLAALAGTLPVVSPAVWDWLDVRAGEPRITQPAVEQFVPQMVNFDVIGAVNFR
CCCCHHHHHHHHHCCCHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCEEEEEEECCC
KGCYPGQEVVARSQYRGTIKRRTALAHVAGETDTVHAGVELFHSDDPGQPCGMIVNAAAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECEEEEECCCCCCCCCCEEECCCC
PAGGVDALVEIKLAALDGGTVHLGSADGPALAFDALPYAWPTEV
CCCCCCEEEEEEEEEECCCEEEECCCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
STPFASPAAQAASASLPVFPRPSAADFDAPAACMPLPQFGVIDVAGDDAATFLHSQLTN
CCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEEECCCHHHHHHHHHHHH
DIEHLDAGSARLSGYCSPKGRLLASFLTWRAGHDVRLLVSKDVQPAVQKRLSMFVLRAKA
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
KLTDASDTLAVAGFAGDVRDALSGIFDALPDGVHVKVDGPAGALIRVPDAAGRKRYLWIG
HCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCCCCCEEEEEEC
PRAEVDARLAALAGTLPVVSPAVWDWLDVRAGEPRITQPAVEQFVPQMVNFDVIGAVNFR
CCCCHHHHHHHHHCCCHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCEEEEEEECCC
KGCYPGQEVVARSQYRGTIKRRTALAHVAGETDTVHAGVELFHSDDPGQPCGMIVNAAAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECEEEEECCCCCCCCCCEEECCCC
PAGGVDALVEIKLAALDGGTVHLGSADGPALAFDALPYAWPTEV
CCCCCCEEEEEEEEEECCCEEEECCCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA