Click here to switch to the map view.

The map label for this gene is pcm [H]

Identifier: 116689843

GI number: 116689843

Start: 2024167

End: 2025099

Strand: Reverse

Name: pcm [H]

Synonym: Bcen2424_1822

Alternate gene names: 116689843

Gene position: 2025099-2024167 (Counterclockwise)

Preceding gene: 116689844

Following gene: 116689842

Centisome position: 58.13

GC content: 70.53

Gene sequence:

>933_bases
ATGAGCGGCGAGCGCGCGAAGCGGTTCCCGCTCGCGCTCGAAGATCTCAAGCGAGCGCCACGCAAGTCGGAAGGTCGGCC
CGGTGAACGCCAGACGGCGGGGGCGGTGCCGAAGGCTGCCGACAAACCCGCGGCCGTGCTGAAGCCGGTTGCGGTGAAGC
CGGCCGCCGTGCGGGCGCCGCTGCCGGGTATCGCCGCCGCGAAGCCGGCGACCGCACCGAAGCCCACCGCGCTGAAGCCT
GCGCTGCCGAAGCCGGCCGCGCCGAGCATCGCGCCGGCCGGCGCGTTCGCCCTCACGTCGGAACGTGTGCGCGAGCGGAT
GGTCGAACGCCTGCGCGCGAACGGCGTGACCGATGCGCGCGTGCTGGACGCAATGGCCGCGGTGCCGCGCCACCTGTTCG
TGGATCCCGGGCTCGCGACGCAGGCCTACGAGGATTCGGCATTGCCGATCGGCCATCAGCAGACCATTTCAAAGCCGTCG
GTCGTCGCGCGCATGATCGAGCTCGCGATGGCCGGCCGCACCCTCGAGCGCGTGCTCGAGATCGGCACGGGTTGCGGCTA
TCAGGCCGCCGTGCTGAGTCACGTGGCACGCGACGTGTATTCGATTGAACGCATCAAGCCGCTTTACGAGCGCGCGAAGC
TGAACCTGCGGCCGCTGCGCGTGCCGAACATCCGTCTGCACTACGGCGACGGGCGTGTCGGCTTGCCGTCCGCGGCCCCG
TTCGACGCGATCGTGATCGCGGCGGCGGGGCTCGACGTGCCGCAGGCGCTGCTCGAGCAGCTCGCGATCGGCGGGCGGCT
CGTCGCGCCGGTCGGCGCGCAGAGCGGGCAGCACCAGGTGCTCACGCTCGTCGAGCGCGTCGCGCACGCGCAATGGCGAG
AGTCCCGGCTTGATCGCGTTTTCTTTGTCCCTTTAAAATCCGGAGTGATTTGA

Upstream 100 bases:

>100_bases
CAAACGGTTTCGTCTCGATCACACCGCTGCAACTCGATCTCACGCATACGCAGATGCTGCCCGCGATGCGCGAATGGGCG
CGCGCCGGAGGGCGGGCTTC

Downstream 100 bases:

>100_bases
AACCGATGAGTATGTTGCGCGCGATGCAAAACAACCGATCCAGGGAACCGCTCACGTTCGCCCAGCGCGCGATCTGTGTG
GCTGCGTTCTCCACGCTACT

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSGERAKRFPLALEDLKRAPRKSEGRPGERQTAGAVPKAADKPAAVLKPVAVKPAAVRAPLPGIAAAKPATAPKPTALKP
ALPKPAAPSIAPAGAFALTSERVRERMVERLRANGVTDARVLDAMAAVPRHLFVDPGLATQAYEDSALPIGHQQTISKPS
VVARMIELAMAGRTLERVLEIGTGCGYQAAVLSHVARDVYSIERIKPLYERAKLNLRPLRVPNIRLHYGDGRVGLPSAAP
FDAIVIAAAGLDVPQALLEQLAIGGRLVAPVGAQSGQHQVLTLVERVAHAQWRESRLDRVFFVPLKSGVI

Sequences:

>Translated_310_residues
MSGERAKRFPLALEDLKRAPRKSEGRPGERQTAGAVPKAADKPAAVLKPVAVKPAAVRAPLPGIAAAKPATAPKPTALKP
ALPKPAAPSIAPAGAFALTSERVRERMVERLRANGVTDARVLDAMAAVPRHLFVDPGLATQAYEDSALPIGHQQTISKPS
VVARMIELAMAGRTLERVLEIGTGCGYQAAVLSHVARDVYSIERIKPLYERAKLNLRPLRVPNIRLHYGDGRVGLPSAAP
FDAIVIAAAGLDVPQALLEQLAIGGRLVAPVGAQSGQHQVLTLVERVAHAQWRESRLDRVFFVPLKSGVI
>Mature_309_residues
SGERAKRFPLALEDLKRAPRKSEGRPGERQTAGAVPKAADKPAAVLKPVAVKPAAVRAPLPGIAAAKPATAPKPTALKPA
LPKPAAPSIAPAGAFALTSERVRERMVERLRANGVTDARVLDAMAAVPRHLFVDPGLATQAYEDSALPIGHQQTISKPSV
VARMIELAMAGRTLERVLEIGTGCGYQAAVLSHVARDVYSIERIKPLYERAKLNLRPLRVPNIRLHYGDGRVGLPSAAPF
DAIVIAAAGLDVPQALLEQLAIGGRLVAPVGAQSGQHQVLTLVERVAHAQWRESRLDRVFFVPLKSGVI

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=214, Percent_Identity=33.6448598130841, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1789100, Length=211, Percent_Identity=45.4976303317536, Blast_Score=161, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI71983477, Length=223, Percent_Identity=32.2869955156951, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI193207222, Length=219, Percent_Identity=31.5068493150685, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17981723, Length=218, Percent_Identity=32.5688073394495, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 32970; Mature: 32839

Theoretical pI: Translated: 11.04; Mature: 11.04

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGERAKRFPLALEDLKRAPRKSEGRPGERQTAGAVPKAADKPAAVLKPVAVKPAAVRAP
CCCCCCHHCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHCCCCCCCHHHCCC
LPGIAAAKPATAPKPTALKPALPKPAAPSIAPAGAFALTSERVRERMVERLRANGVTDAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
VLDAMAAVPRHLFVDPGLATQAYEDSALPIGHQQTISKPSVVARMIELAMAGRTLERVLE
HHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IGTGCGYQAAVLSHVARDVYSIERIKPLYERAKLNLRPLRVPNIRLHYGDGRVGLPSAAP
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEECCCCCCCCCCCC
FDAIVIAAAGLDVPQALLEQLAIGGRLVAPVGAQSGQHQVLTLVERVAHAQWRESRLDRV
HHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCEE
FFVPLKSGVI
EEEECCCCCC
>Mature Secondary Structure 
SGERAKRFPLALEDLKRAPRKSEGRPGERQTAGAVPKAADKPAAVLKPVAVKPAAVRAP
CCCCCHHCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHCCCCCCCHHHCCC
LPGIAAAKPATAPKPTALKPALPKPAAPSIAPAGAFALTSERVRERMVERLRANGVTDAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
VLDAMAAVPRHLFVDPGLATQAYEDSALPIGHQQTISKPSVVARMIELAMAGRTLERVLE
HHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IGTGCGYQAAVLSHVARDVYSIERIKPLYERAKLNLRPLRVPNIRLHYGDGRVGLPSAAP
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEECCCCCCCCCCCC
FDAIVIAAAGLDVPQALLEQLAIGGRLVAPVGAQSGQHQVLTLVERVAHAQWRESRLDRV
HHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCEE
FFVPLKSGVI
EEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA