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The map label for this gene is purA1 [H]

Identifier: 116689822

GI number: 116689822

Start: 2001905

End: 2003251

Strand: Reverse

Name: purA1 [H]

Synonym: Bcen2424_1801

Alternate gene names: 116689822

Gene position: 2003251-2001905 (Counterclockwise)

Preceding gene: 116689823

Following gene: 116689821

Centisome position: 57.5

GC content: 65.78

Gene sequence:

>1347_bases
ATGTCTGCCAGCGCAGTGAACGTGACTCCCGGGCGCAATGTCGTCGTCGTGGGAACGCAATGGGGTGATGAAGGCAAGGG
CAAGATCGTCGACTGGCTGACGGACCACGCTCAGGGCGTCGTGCGTTTCCAGGGCGGTCACAACGCCGGCCACACCCTCA
TCATCGGCGGCAAGAAAACGATCTTGCGCCTCATTCCGTCGGGCATCATGCGTGAAGGCGTCGCCTGCTACATCGGCAAC
GGCGTCGTTCTGTCCCCCGAAGCACTGTTCAAGGAAATCGGCGAGCTCGAAGAAGCCGGCGTGAACGTGCGCGACCGTCT
GTTCATCTCCGAAGCCACGACGCTGATCCTGCCGTACCACATCGCGATCGACCAGGCGCGCGAAGCGCGCAAGGGCGCGG
GCAAGATCGGCACGACGGGCCGCGGCATCGGCCCTGCGTACGAAGACAAGGTCGGCCGCCGCGCGCTGCGCGTGCAGGAC
CTGTTCGACGCGAAGACCTTCGCCGATCGCCTGCGCGAAAATCTCGATTACCACAACTTCGTGCTGACCCAGTATCTGGG
CGGCGCGGCCGTCGACTTCCAGGCCACGCTCGATACGATGCTCGGCTATGCCGACCGCCTGAAGCCGATGGTGGCCGACG
TGTCGCGCCGCCTGTACGACGCGAACAATGCGGGCCAGAACCTGCTGTTCGAAGGTGCGCAAGGCACGCTGCTCGACATC
GACCACGGCACCTATCCGTTCGTCACGTCGAGCAACTGCGTCGCCGGTGCGGCGTCGGCTGGCGCGGGCGTCGGTCCGCA
GAAGCTCAACTACATTCTGGGCATCACGAAGGCGTATTGCACGCGCGTCGGCTCGGGCCCGTTCCCGAGCGAGCTGTACG
ATGCGGACAACCCGCAGCGCCAGGACCAGATCGGCGTCACGCTCGCGAACGTCGGCAAGGAATTCGGCTCGGTCACCGGC
CGTCCGCGCCGCACCGGCTGGCTCGATGCGGCTGCGCTGCGCCGCTCGATCCAGATCAACGGCGTGTCGGGCCTGTGCAT
GACGAAGCTCGACGTGCTCGACGGCCTCGACGAAGTGAAGCTGTGCGTCGGCTACAAGGTCGACGGCAAGGACGTGGACA
TCCTGCCGCGTGGCGCGGTCGATGTCGCGCGTTGCGAACCGGTGTACGAGACGTTCGCCGGCTGGAAGGAAAGCACGGTC
GGGATCAACACGTGGGACGCACTGCCGGCGAACGCGCAGGCCTACCTCGCACGCGTCCAGGAAGTGGCTGGCGTGCCGGT
CGACATGGTGTCGACGGGCCCGGACCGCGACGAAACGATCCTGCTCCGCCATCCGTTCAAGGTGTAA

Upstream 100 bases:

>100_bases
GGCCGTGCTTCGGCGTGCATATCGTTGAAGCGAAACGGAAAAATTCGGAATCGCCCGCGAACATAGGTAGAATACGTTTT
TAACCAGCTAACGAATCAAC

Downstream 100 bases:

>100_bases
TTCCGAGATGACGCAGCAAATCACGATGACGGCGGCAGACTTGATGACCGATCCGCGCAACGACGACAAGAACCTGTGGG
TAGGCTGGGACGAGTATCAT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase 1; AdSS 1; IMP--aspartate ligase 1 [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSASAVNVTPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMREGVACYIGN
GVVLSPEALFKEIGELEEAGVNVRDRLFISEATTLILPYHIAIDQAREARKGAGKIGTTGRGIGPAYEDKVGRRALRVQD
LFDAKTFADRLRENLDYHNFVLTQYLGGAAVDFQATLDTMLGYADRLKPMVADVSRRLYDANNAGQNLLFEGAQGTLLDI
DHGTYPFVTSSNCVAGAASAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNPQRQDQIGVTLANVGKEFGSVTG
RPRRTGWLDAAALRRSIQINGVSGLCMTKLDVLDGLDEVKLCVGYKVDGKDVDILPRGAVDVARCEPVYETFAGWKESTV
GINTWDALPANAQAYLARVQEVAGVPVDMVSTGPDRDETILLRHPFKV

Sequences:

>Translated_448_residues
MSASAVNVTPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMREGVACYIGN
GVVLSPEALFKEIGELEEAGVNVRDRLFISEATTLILPYHIAIDQAREARKGAGKIGTTGRGIGPAYEDKVGRRALRVQD
LFDAKTFADRLRENLDYHNFVLTQYLGGAAVDFQATLDTMLGYADRLKPMVADVSRRLYDANNAGQNLLFEGAQGTLLDI
DHGTYPFVTSSNCVAGAASAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNPQRQDQIGVTLANVGKEFGSVTG
RPRRTGWLDAAALRRSIQINGVSGLCMTKLDVLDGLDEVKLCVGYKVDGKDVDILPRGAVDVARCEPVYETFAGWKESTV
GINTWDALPANAQAYLARVQEVAGVPVDMVSTGPDRDETILLRHPFKV
>Mature_447_residues
SASAVNVTPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMREGVACYIGNG
VVLSPEALFKEIGELEEAGVNVRDRLFISEATTLILPYHIAIDQAREARKGAGKIGTTGRGIGPAYEDKVGRRALRVQDL
FDAKTFADRLRENLDYHNFVLTQYLGGAAVDFQATLDTMLGYADRLKPMVADVSRRLYDANNAGQNLLFEGAQGTLLDID
HGTYPFVTSSNCVAGAASAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNPQRQDQIGVTLANVGKEFGSVTGR
PRRTGWLDAAALRRSIQINGVSGLCMTKLDVLDGLDEVKLCVGYKVDGKDVDILPRGAVDVARCEPVYETFAGWKESTVG
INTWDALPANAQAYLARVQEVAGVPVDMVSTGPDRDETILLRHPFKV

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=440, Percent_Identity=42.2727272727273, Blast_Score=325, Evalue=6e-89,
Organism=Homo sapiens, GI34577063, Length=443, Percent_Identity=39.2776523702032, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI40316944, Length=405, Percent_Identity=40.9876543209877, Blast_Score=289, Evalue=4e-78,
Organism=Escherichia coli, GI1790620, Length=437, Percent_Identity=60.1830663615561, Blast_Score=539, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI25146553, Length=436, Percent_Identity=39.6788990825688, Blast_Score=307, Evalue=6e-84,
Organism=Caenorhabditis elegans, GI25146555, Length=436, Percent_Identity=39.6788990825688, Blast_Score=307, Evalue=7e-84,
Organism=Saccharomyces cerevisiae, GI6324109, Length=437, Percent_Identity=40.045766590389, Blast_Score=317, Evalue=2e-87,
Organism=Drosophila melanogaster, GI21356033, Length=456, Percent_Identity=39.0350877192982, Blast_Score=313, Evalue=2e-85,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 48147; Mature: 48016

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASAVNVTPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKT
CCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHH
ILRLIPSGIMREGVACYIGNGVVLSPEALFKEIGELEEAGVNVRDRLFISEATTLILPYH
HHHHHHHHHHHCCCEEEECCCEEECHHHHHHHHHHHHHCCCCHHHEEEEECCCEEEEEEH
IAIDQAREARKGAGKIGTTGRGIGPAYEDKVGRRALRVQDLFDAKTFADRLRENLDYHNF
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VLTQYLGGAAVDFQATLDTMLGYADRLKPMVADVSRRLYDANNAGQNLLFEGAQGTLLDI
HHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEC
DHGTYPFVTSSNCVAGAASAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNPQR
CCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCC
QDQIGVTLANVGKEFGSVTGRPRRTGWLDAAALRRSIQINGVSGLCMTKLDVLDGLDEVK
HHHHCEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCEE
LCVGYKVDGKDVDILPRGAVDVARCEPVYETFAGWKESTVGINTWDALPANAQAYLARVQ
EEEEEEECCCCCEECCCCCCCHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
EVAGVPVDMVSTGPDRDETILLRHPFKV
HHHCCCEEEECCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
SASAVNVTPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKT
CCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHH
ILRLIPSGIMREGVACYIGNGVVLSPEALFKEIGELEEAGVNVRDRLFISEATTLILPYH
HHHHHHHHHHHCCCEEEECCCEEECHHHHHHHHHHHHHCCCCHHHEEEEECCCEEEEEEH
IAIDQAREARKGAGKIGTTGRGIGPAYEDKVGRRALRVQDLFDAKTFADRLRENLDYHNF
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VLTQYLGGAAVDFQATLDTMLGYADRLKPMVADVSRRLYDANNAGQNLLFEGAQGTLLDI
HHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEC
DHGTYPFVTSSNCVAGAASAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNPQR
CCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCC
QDQIGVTLANVGKEFGSVTGRPRRTGWLDAAALRRSIQINGVSGLCMTKLDVLDGLDEVK
HHHHCEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCEE
LCVGYKVDGKDVDILPRGAVDVARCEPVYETFAGWKESTVGINTWDALPANAQAYLARVQ
EEEEEEECCCCCEECCCCCCCHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
EVAGVPVDMVSTGPDRDETILLRHPFKV
HHHCCCEEEECCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA