Click here to switch to the map view.

The map label for this gene is dmlR [H]

Identifier: 116689798

GI number: 116689798

Start: 1975033

End: 1975983

Strand: Direct

Name: dmlR [H]

Synonym: Bcen2424_1777

Alternate gene names: 116689798

Gene position: 1975033-1975983 (Clockwise)

Preceding gene: 116689795

Following gene: 116689799

Centisome position: 56.69

GC content: 72.03

Gene sequence:

>951_bases
ATGACGTCCGATCCCGGGTCCGATCCGATTCCCGCGCCCGACAAGCCGCTCGACCTGCTCGACGTCGCGCTGTTCGTGCG
CGCGGCGCTGCTCGCGAACGTCACCGCGGCCGGGCGCGAATTCGGCGTGTCGGCCGCGGTCGCGAGCGCGCGCATCGCCC
AGCTCGAACGGCAGCTCGGCGCGCGGCTGCTGCACCGCACCACGCGCCGCATCAGCCTCACGCAGGACGGCGAAGTCTTC
ATGGCGCGCGCCGACGCGCTGCTGGCGGCCGCCGACGCCGCGCGCGCGTCGGTCGGCCGCGGCCGCAGCGAGCCGTACGG
GCGGCTGAAGGTGTCGATGTCGTCGTCGTTCGGCCGCCAGCACGTCGCGCCGGTGATTCCGGCGTTCCTGCGCCGCTACC
CGTCCGTGTCGCTCGATCTGCGGCTGTCCGACGAGATCGTCGATCTGGTCGACGACGGCTACGACGTCGCGATCCGGCTC
GGCGCGCTGAAGGATTCGACGCTCGTCGCCCGCAAGCTCGCCACCAACCGCCGCGTGCTGTGCTGCTCGCCCGCCTATCT
CGCCGAACGCGGCACGCCGCGCCATCCGGCCGACTTGACGCAGCACGAATGCGTGATCCTCGGCGACCAGCGCGACTGGT
CGTTCGCGACGCCGCAGGGCCCGCTCACGGTGCGGGTCGGCGGCCGGCTCGTCGCGAGCAACGGCGAGGCGATCCGCGAA
GCGCTCGTCGACGGCTTCGGGATCGCGATCAAGTCGACCTGGGACGTCGGCCCGCTGCTCGCGAGCGGCGCGCTCGTCAG
CGTGCTCGACGACTACCCGTTCGCGGACGACGTCGCGATCTGGGCCGTCTATCCGAGCCGCGCGCACGTGCCGCTGAAAA
CGCTCGCGTTCATTGCGTTCCTGGCCGAGCACTTCGGCGATCCGCCGTACTGGGATCGTGCAAACGGCTGA

Upstream 100 bases:

>100_bases
GGCAGGCTGCGGCGCAACCTCCATGCGTGCCATCGTAGGCAACCCGATCCTTCGAGAAAACCGCTATATTTCCTGAAACA
TTTTCAGGAATTTCAGAATA

Downstream 100 bases:

>100_bases
CCACTACCGCCGCGCGCCCGGTTTCGCGCCGGCGCCGGCGCCCTGCGCGGCCAACCGGCTACAATGGCGCGTTCGCGACG
CAACGGCCCGCAGCCGCACG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTSDPGSDPIPAPDKPLDLLDVALFVRAALLANVTAAGREFGVSAAVASARIAQLERQLGARLLHRTTRRISLTQDGEVF
MARADALLAAADAARASVGRGRSEPYGRLKVSMSSSFGRQHVAPVIPAFLRRYPSVSLDLRLSDEIVDLVDDGYDVAIRL
GALKDSTLVARKLATNRRVLCCSPAYLAERGTPRHPADLTQHECVILGDQRDWSFATPQGPLTVRVGGRLVASNGEAIRE
ALVDGFGIAIKSTWDVGPLLASGALVSVLDDYPFADDVAIWAVYPSRAHVPLKTLAFIAFLAEHFGDPPYWDRANG

Sequences:

>Translated_316_residues
MTSDPGSDPIPAPDKPLDLLDVALFVRAALLANVTAAGREFGVSAAVASARIAQLERQLGARLLHRTTRRISLTQDGEVF
MARADALLAAADAARASVGRGRSEPYGRLKVSMSSSFGRQHVAPVIPAFLRRYPSVSLDLRLSDEIVDLVDDGYDVAIRL
GALKDSTLVARKLATNRRVLCCSPAYLAERGTPRHPADLTQHECVILGDQRDWSFATPQGPLTVRVGGRLVASNGEAIRE
ALVDGFGIAIKSTWDVGPLLASGALVSVLDDYPFADDVAIWAVYPSRAHVPLKTLAFIAFLAEHFGDPPYWDRANG
>Mature_315_residues
TSDPGSDPIPAPDKPLDLLDVALFVRAALLANVTAAGREFGVSAAVASARIAQLERQLGARLLHRTTRRISLTQDGEVFM
ARADALLAAADAARASVGRGRSEPYGRLKVSMSSSFGRQHVAPVIPAFLRRYPSVSLDLRLSDEIVDLVDDGYDVAIRLG
ALKDSTLVARKLATNRRVLCCSPAYLAERGTPRHPADLTQHECVILGDQRDWSFATPQGPLTVRVGGRLVASNGEAIREA
LVDGFGIAIKSTWDVGPLLASGALVSVLDDYPFADDVAIWAVYPSRAHVPLKTLAFIAFLAEHFGDPPYWDRANG

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=294, Percent_Identity=38.7755102040816, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1789440, Length=297, Percent_Identity=31.6498316498317, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=33.1058020477816, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=28.8659793814433, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=29.5138888888889, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI145693193, Length=286, Percent_Identity=31.1188811188811, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1787589, Length=282, Percent_Identity=25.531914893617, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1789173, Length=277, Percent_Identity=26.7148014440433, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786448, Length=281, Percent_Identity=28.8256227758007, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788706, Length=192, Percent_Identity=28.6458333333333, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33961; Mature: 33830

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDPGSDPIPAPDKPLDLLDVALFVRAALLANVTAAGREFGVSAAVASARIAQLERQLG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHH
ARLLHRTTRRISLTQDGEVFMARADALLAAADAARASVGRGRSEPYGRLKVSMSSSFGRQ
HHHHHHHHHEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHH
HVAPVIPAFLRRYPSVSLDLRLSDEIVDLVDDGYDVAIRLGALKDSTLVARKLATNRRVL
HHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCEEE
CCSPAYLAERGTPRHPADLTQHECVILGDQRDWSFATPQGPLTVRVGGRLVASNGEAIRE
EECCHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCEEEECCCHHHHH
ALVDGFGIAIKSTWDVGPLLASGALVSVLDDYPFADDVAIWAVYPSRAHVPLKTLAFIAF
HHHCCCCEEEECCCCCHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHH
LAEHFGDPPYWDRANG
HHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TSDPGSDPIPAPDKPLDLLDVALFVRAALLANVTAAGREFGVSAAVASARIAQLERQLG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHH
ARLLHRTTRRISLTQDGEVFMARADALLAAADAARASVGRGRSEPYGRLKVSMSSSFGRQ
HHHHHHHHHEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHH
HVAPVIPAFLRRYPSVSLDLRLSDEIVDLVDDGYDVAIRLGALKDSTLVARKLATNRRVL
HHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCEEE
CCSPAYLAERGTPRHPADLTQHECVILGDQRDWSFATPQGPLTVRVGGRLVASNGEAIRE
EECCHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCEEEECCCHHHHH
ALVDGFGIAIKSTWDVGPLLASGALVSVLDDYPFADDVAIWAVYPSRAHVPLKTLAFIAF
HHHCCCCEEEECCCCCHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHH
LAEHFGDPPYWDRANG
HHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]